Humate在小葱上施用效果研究

通过盆栽试验,研究了Humate在小葱上的施用效果,试验结果表明:Humate对小葱产量的提高和分蘖能力具有明显的促进作用。各次收获结果的差异显示出Humate的后效,同时显示出Humate与其他营养成份配合的重要性。

The biological potency of benzapyrene in the humates composition

The aim of the paper was to determine benz(a)-pyrene in the preparations containing humates and study the benz(a)pyrene biological potency for the agricultural plants. The research methodology included the determination of the dependencies in the system “substance concentration (dose)—effect on the plant”. Concentrations of benz(a)pyrene in 12 samples of the humates preparations and fertilizers based on their trademarks “Irkutsk humates”, obtained from brown coal, varied in the range from 0.3 to 50 mcg/kg, which creates no soil contamination in conditions of the use of preparations. Between contents of benz(a)pyrene and humates there is a correlation (rxy = 0.95;α = 0.05). It is ascertained that the effects of stimulation and/or inhibition of the growth and yield of agricultural plants depend on the concentration of benz(a)pyrene and the method of plant processing. Optimal concentrations of benz(a)pyrene were 150-200 ng/dm3 for preplant way of processing of potato tubers, 3-10 ng/dm3—for top dressing (spraying) and 0.1-0.3 ng/dm3—for dressing under the roots (hydroponic). The obtained results allowed us to offer one of the possible mechanisms of biological potency of humates as the plant growth stimulants, and also a way testing of the preparations by screening of their benz(a)pyrene content.

Sodium humate accelerates cutaneous wound healing by activating TGF-β/Smads signaling pathway in rats

Sodium humate(HA-Na) has been topically used as a wound healing and anti-inflammatory agent in folk medicine. In the present study, HA-Na was investigated for cutaneous wound healing in Sprague–Dawley rats. HA-Na solution(1.0%, w/v) was topically administered to rats undergoing excision wound models. Healing was assessed with a recombinant bovine basic fibroblast growth factor for external use as positive control. Wound healing rates were calculated on Day 3, 6, 9, 14 and 21 after injury, and tissues were also harvested after the same intervals for histological analysis. In addition, tissue hydroxyproline levels were measured. Furthermore, m RNA levels and protein expressions of transforming growth factor-β1, 2, 3(TGF-β1, 2, 3) were determined by RT-PCR and western blot. Protein expression levels of Smad-2,-3,-4 and-7 were also detected by western blot. Our study demonstrates that HA-Na has the capacity to promote wound healing in rats via accelerated wound contraction and increased hydroxyproline content. More importantly, these wound healing effects of HA-Na might be mediated through the TGF-β/Smad signaling pathway. HA-Na may be an effective agent for enhanced wound healing.

Micropropagation of Curcuma Sp., a Threatened Medicinal Plant

A rapid and improved micropropagation protocol was developed for Curcuma sp., a threatened and high value medicinal plant, using main bud sprout and top of rhizome sprout as explants. Stepwise optimization of different plant is to change the growth regulator, reduce the level of macro-nutrition and add humate. The present study has created multiple shoot and root induction and plantlet in vitro culture, transfers the plantlet to ex vitro. The M2 medium (MS’s macronutrition 1/4, MS’s micronutrition + Morel’s vitamine + coconut milk 10% + sucrose 25 g/l + humate 1.0 ml/l + agar 7.5 g/l + 2,4D 0.5 mg/l + BAP1.0 mg/l + TDZ 1.0 mg/l) is the highest ratio of callus induction. The TA3 medium (MS’s macronutrition 1/4 + MS’s micronutrition + Morel’s vitamin+ coconut milk 10% + sucrose 25g/l + humate 2.0 ml/l + 2,4 D 0.5 mg/l + Kinetin 2.0 mg/l + TDZ 1.0 mg/l + BAP 1.0 mg/l2 + NAA 2.0 mg/l + activated carbon 2.0 g/l) is able to create buds and regeneration multiple bud for Curcuma sp. TA3 medium adding IAA 2 mg/l and IBA 0.5 mg/l has resulted in the highest indices of quantity, healthy shoot and large diameter of roots. A large number of healthy plantlets are induced by the medium of MS’s macronutrients 1/2, MS’s micronutrients full, Morel’s vitamin, humate 3 g/l, coconut milk 150 ml/l, activated carbon 3 g/l, composition phytohormone: IAA 2 mg/l + BAP 2 mg/l + TDZ 0.5 mg/l. Further studies should focus on optimizing the humate concentration for these species of Zingiberaceae. The duration of the research is from 5/2015 to 4/2016 at Faculty of Agriculture and Forestry, Tay Nguyen University, Vietnam.

pH-sensitive KHA/CMC-Fe^(3+)@CS hydrogel loading and the drug release properties of riboflavin

To improve drug utilization,reduce the drug administration frequency,increase the release time,and reduce the drug side effects in the human body,we prepared(KHA/CMC-Fe^(3+))@CS hydrogel spheres using green and natural potassium humate(KHA),carboxymethyl cellulose(CMC),and chitosan(CS)as raw materials and Fe3+as a crosslinking agent,and loaded them with riboflavin for drug sustainedrelease study using the drop ball method.The tests with FTIR,SEM,TG,and X-ray diffractometer showed that the coordination among KHA,CMC,and Fe^(3+)formed a three-dimensional network structure,where cs was encapsulated on the surface of the hydrogel spheres via noncovalent bonding,resulting in good thermal stability.The stability,drug loading,swelling,and in vitro release of the(KHA/CMC-Fe^(3+))@CS hydrogel spheres were investigated.The results showed that the hydrogel spheres were significantly pH-sensitive,with 11.16 g/g higher swelling in an alkaline environment(pH=7.4)than that in an acidic environment(pH=1.2).The swelling and drug release process of the hydrogel spheres were analyzed using mathematical models,concluding that the hydrogel swelling follows Schott second-order swelling kinetics,and the drug release mechanism was Fickian delivery mode.