Effects of urea enhanced with different weathered coal-derived humic acid components on maize yield and fate of fertilizer nitrogen

Humic acid(HA) is a readily available and low-cost material that is used to enhance crop production and reduce nitrogen(N) loss. However, there is little consensus on the efficacy of different HA components. In the current study, a soil column experiment was conducted using the ^(15)N tracer technique in Dezhou City, Shandong Province, China, to compare the effects of urea with and without the addition of weathered coal-derived HA components on maize yield and the fate of fertilizerderived N(fertilizer N). The HA components were incorporated into urea by blending different HA components into molten urea to obtain the three different types of HA-enhanced urea(HAU). At harvest, the aboveground dry biomass of plants grown with HAU was enhanced by 11.50–21.33% when compared to that of plants grown with U. More significantly, the grain yields under the HAU treatments were 5.58–18.67% higher than the yield under the urea treatment. These higher yields were due to an increase in the number of kernels per plant rather than the weight of individual kernels. The uptake of fertilizer N under the HAU treatments was also higher than that under the urea treatment by 11.49–29.46%, while the unaccounted N loss decreased by 12.37–30.05%. More fertilizer-derived N was retained in the 0–30 cm soil layer under the HAU treatments than that under the urea treatment, while less N was retained in the 30–90 cm soil layer. The total residual amount of fertilizer N in the soil column, however, did not differ significantly between the treatments. Of the three HAU treatments investigated, the one with an HA fraction derived from extraction with pH values ranging from 6 to 7, resulted in the best improvement in all assessment targets. This is likely due to the abundance of the COO/C–N=O group in this HA component.

Effects of Plant-derived Humic Acid Organic Fertilizer on Selenium Content of Rice in Northern Guangxi

This study was to find out the suitable application amount of plant-derived humic acid organic fertilizer for selenium-rich rice production in northern Guilin area by applying the fertilizer at different amounts.The results showed that plant-derived humic acid organic fertilizer could effectively activate selenium in selenium-rich soil,and then promote the uptake of selenium by rice.With costs,selenium enrichment and rice yield as the main evaluation indicators,A4 was chosen as the best treatment,that is,the application of 4 500 kg/hm^2 of plant-derived humic acid organic fertilizer as base fertilizer could achieve the best benefit.

Effects of Nano-carbon Humic Acid Water-retaining Fertilizer on Citrus Growth and the Soil Bacterial Community in Citrus Field

[Objective] In order to reveal the effects of reducing the amount of novel nano-carbon humic acid water-retaining fertilizer(CSF) on soil microbial community structure and citrus growth. [Method]In this study,conventional fertilization was as the control(KC1) in Wanzhou citrus orchard of Three Gorges Reservoir area. CSF reductions by 0%(KC2),10%(KC3),20%(KC4),30%(KC5) and 40%(KC6) were used to analyze the changes of soil bacterial community structure,citrus yield and quality. [Result]The results showed that the observed species,Shannon index,Chao1 index and PD_whole_tree of KC6 were higher than those of KC1,and were the same as KC2. The abundance of Xanthomonadaceae was the highest in KC5. Compared with KC1,the Xanthomonadaceae in KC3,KC4 and KC6 was significantly decreased,and the levels of Nitrosomonadaceae and Pseudomonasaceae were higher than that of KC1 after the treatment of KC6. Sphingomonas in different reduction treatments was lower than that of KC1,but Burkholderia and Pseudomonas were significantly higher than those of KC1. It was found that the similarity among treatments was small after bacterial community similarity clustering analysis,and citrus yield increased somewhat after CSF fertilization reduction.When CSF fertilization reduced by 30%,citrus yield increased by 4. 50%. When CSF fertilization reduced by 40%,citrus yield decreased by4. 14%. After CSF fertilization,citrus quality did not change significantly in CSF conventional fertilization and reduction of 10% and 40%,while significantly decreased in 20% and 30% of fertilization reduction. [Conclusion] CSF fertilization reduction changed the diversity of soil bacterial community structure and the yield and quality of citrus.

Experiment of Water Soluble Fertilizers Containing Humic Acid in Tomato Planting

As a natural macromolecular organic compound,humic acid can promote plant growth.Water soluble humic acid fertilizers refer to liquid or solid water soluble fertilizers made by adding appropriate amounts of nitrogen,phosphorus,potassium macroelements or zinc,boron,iron,molybdenum,manganese,copper and other trace elements or organic matter,based on humic acid in the required proportions for plant growth.In order to verify the application effect of humic acid containing water soluble fertilizer produced by Tai’an Gold Land Chemical Co.,Ltd.,in tomato production in Daiyue District,Tai’an City,a fertilizer effect application experiment of humic acid containing water soluble fertilizer on tomato planting was specially carried out.The results showed that the water soluble fertilizer containing humic acid could obviously improve the agronomic characters of tomato;the yield was significantly increased;the effect of increasing the income of tomato growers was obvious.

Carbon Sequestration in Soil Humic Substances Under Long-Term Fertilization in a Wheat-Maize System from North China

The changes in humic substances （HS） is fundamental in detecting soil carbon sequestration mechanisms in natural and cultivated environments. Based on a long-term trial, the amounts of water dissolved substances （WSS）, humic acid （HA）, fulvic acid （FA） and humin （HU） were determined to explore the impact of long-term fertilization on HS. Increases in the amounts of WSS, HA, FA and HU were significant different among the treatments with manure. A significant correlation was found between the increased soil organic carbon （SOC） and HS （R^2=0.98, P〈0.01）. The change in the E4/E6 ratio was significantly correlated with the increased SOC （R2=0.88, P〈0.01）, HA （R^2=0.91, P〈0.01）, FA （R^2=0.91, P〈0.01） and HU （R^2=0.88, P〈0.01）. The cluster was mainly divided into two parts as manure fertilization and inorganic fertilization, based on the increases in HA, FA and HU. These results suggest that long term fertilization with manure favours carbon sequestration in HS and is mainly stabilized as HU, while the HA becomes more aliphatic. We conclude that increases in SOC can be linked to changes in the molecular characteristics of HS fractions under long term fertilization.

微咸水-腐植酸肥耦合滴灌条件下钙镁离子质量浓度对灌水器堵塞的影响

【目的】探明腐植酸肥施用条件下不同钙镁离子质量浓度对灌水器堵塞物质形成的影响效应与作用机制。【方法】以微咸水中钙镁离子耦合腐植酸肥滴灌为研究对象,选取4种不同额定流量(1.6、1.1、1.4、1.75 L/h)的非压力补偿内镶贴片式灌水器(FE1—FE4),其中设置3组钙离子质量浓度微咸水处理,离子质量浓度分别为100、150、200 mg/L(G1、G2、G3),3组镁离子质量浓度微咸水处理,离子质量浓度分别为100、150、200 mg/L(M1、M2、M3),以地下微咸水灌溉为对照(CK),研究不同离子质量浓度的灌水器平均流量(Dra)、滴灌系统灌水器的堵塞率分布、灌水器堵塞物质干质量(DW)动态变化规律,并分析了灌水器内部堵塞物质矿物组分。【结果】与CK相比,G1、G2、G3、M1、M2、M3处理的Dra分别降低了21.58%~29.68%、35.02%~39.71%、45.62%~55.68%、14.25%~20.41%、24.89%~45.69%、35.22%~56.75%,堵塞物质干质量分别增加了124.62%~178.49%、174.23%~230.33%、235.59%~270.09%、67.14%~120.28%、136.96%~191.18%、203.54%~213.35%。与G1、G2、G3处理相比,质量浓度相同镁离子处理M1、M2、M3处理的堵塞物质干质量分别降低了16.41%~27.26%、4.77%~13.60%、6.82%~15.34%。【结论】钙镁离子质量浓度的增加均显著加剧了灌水器的堵塞;在相同离子质量浓度条件下,镁离子处理显著减少了灌水器内堵塞物质的总量,因此镁离子在一定程度上能够降低灌水器堵塞风险。

水解-氧化法制备甘蔗叶生物质腐植酸

本研究以甘蔗叶为原料,通过设计模拟矿源腐植酸形成过程中的化学反应,研究将甘蔗叶转化为腐植酸的技术,并将实验室制得的腐植酸与矿源腐植酸和木质素进行比较研究。制备过程:首先进行酸水解反应,将其中的纤维素和半纤维素水解为可溶解的水解糖,将木质素转化为不溶的腐植酸前体,固液分离;然后用氧化剂对腐植酸前体进行氧化反应,重新生成亲水性的羧基基团,完成腐植酸的制备。实验结果表明,水解反应步骤时,在固定20∶50(g∶mL)固液比的条件下,最佳的反应条件为:10%的硫酸和1%的硝酸组成的水解液,在150℃的温度下,水解3 h;氧化步骤中,腐植酸前体以1∶13(g∶mL)的固液比与3.5%的硝酸在80℃的温度下反应2 h。经元素分析、酸性基团分析、紫外和红外光谱以及核磁共振波谱等一系列分析手段,证实制备的蔗叶生物质腐植酸有较高的腐殖化和芳香化程度,其各项指标均接近于矿源腐植酸。该方法能成功制备腐植酸,同时利用产生的水解糖还具备与多种现有生物质产业相结合的潜在可能,以解决其水解残渣的问题。

噻苯隆作用下叶面肥养分组成变化对小麦生理代谢和产品要素的影响

为探究噻苯隆作用下叶面肥养分组成变化孕穗期喷施对小麦生理代谢、氮素吸收利用以及籽粒产量和品质的影响,以小麦“烟农19号”为试验材料,设置8个盆栽试验处理:A0(3.3mg·L^(-1)噻苯隆)、A1(3.3mg·L^(-1)噻苯隆+腐殖酸叶面肥)、A2(3.3 mg·L^(-1)噻苯隆+缺氮腐殖酸叶面肥)、A3(3.3 mg·L^(-1)噻苯隆+缺磷腐殖酸叶面肥)、A4(3.3 mg·L^(-1)噻苯隆+缺钾腐殖酸叶面肥)、A5(3.3 mg·L^(-1)噻苯隆+缺氨基酸腐殖酸叶面肥)、A6(3.3 mg·L^(-1)噻苯隆+缺硼腐殖酸叶面肥)和A7(3.3 mg·L^(-1)噻苯隆+缺腐殖酸叶面肥),每个处理重复3次。于2022年4月在小麦孕穗期后的1 d、5 d和9 d开始喷施,每次喷施量为150 mL。结果表明:叶面施肥能够提高叶片中叶绿素含量,但对类胡萝卜素含量影响不显著,同时促进可溶性糖的合成和抗氧化酶活性提高,其中氮、磷养分缺失会抑制过氧化氢酶活性,较A1处理降低了26.40%~31.12%;对于氮代谢,叶面施肥能够促进小麦对氮素的吸收和转化,其中氮、磷和硼缺失会降低叶片可溶性蛋白含量,抑制谷氨酸合成酶的活性,缺钾则会抑制叶片谷氨酰胺合成酶活性,相比于A1处理活性降低了20.38%。叶面施肥能够不同程度地提高小麦籽粒产量,增加穗粒数和千粒重,但对总穗数影响不显著,而钾和氨基酸缺失则会降低小麦穗粒数;同时能够改善籽粒品质,增加籽粒中粗蛋白及蛋白组分含量,但影响不显著,而钾、氨基酸和硼缺失影响籽粒中清蛋白和球蛋白的合成。在噻苯隆的作用下,叶面肥中氮、磷、钾元素组成对小麦生理效应影响显著。研究结果为叶面肥养分配比的选择提供基础理论支持。

腐植酸复合肥对玉米生长及养分吸收的影响

为了解不含腐植酸尿素和添加了不同剂量腐植酸的复合肥对玉米生长及养分吸收的影响,研究设定不同的肥料梯度和肥料配比,以探明腐植酸肥料的增产效果和养分利用率.以玉米为试验材料,设无氮处理、复合肥处理、1‰腐植酸+复合肥处理、2‰腐植酸+复合肥处理、3‰腐植酸+复合肥处理、4‰腐植酸+复合肥处理、5‰腐植酸+复合肥处理、6‰腐植酸+复合肥处理共8个施肥处理.试验结果表明,4‰腐植酸复合肥较无氮处理增产率达90%,百粒质量最高,含淀粉量达59.30%,在处理中最具有优势.从综合情况来看,在夏玉米栽培中,加入4‰腐植酸复合肥更有利于玉米增产增效以及贮藏,能获得更大的生物产量和经济效益.

施用不同品种氮肥对麦套花生生长和产量的影响

为研究砂质土壤上追施不同类型氮肥对麦套花生生长发育和产量的影响,在河南新乡市开展田间试验,分别设置不施肥(对照)、施用氯化铵、硫酸铵、硝酸铵钙、普通尿素、腐植酸尿素、控释尿素共7个处理,分析不同处理对花生株高、分枝数、复叶数、干物质和产量的影响。结果表明,与对照相比,施用不同类型氮肥均提高了花生的株高,其中尿素处理的株高在各个时期均较高;腐植酸尿素和控释尿素处理的株高在生育后期较高。施用氮肥能促进花生分枝,下针期以前以氯化铵、尿素和控释尿素处理的增幅较大。氮肥类型显著影响花生复叶数,其中硫酸铵和硝酸铵钙处理花生的复叶数在生育前期较少而后期较多;腐植酸尿素和控释尿素处理在前期较多但成熟期较少;氯化铵和尿素处理在整个生育期则较为平稳。施用各类型氮肥均显著提高了花生膨果期和成熟期的干物质量。与对照相比,追施不同类型氮肥可增产25.0%~61.0%,其中硝酸铵钙和腐植酸尿素增产幅度最大;尿素和控释尿素的增产效果高于硫酸铵和氯化铵;且施用硝酸铵钙、尿素和控释尿素处理花生的坏果率低于其他处理。综上所述,本研究条件下,麦套花生追施氮肥可促进花生生长并提高产量,其中硝酸铵钙的增产效果最佳;腐植酸尿素和控释尿素也具有较好效果。

腐植酸水溶肥在小麦田上的应用效果研究

[目的]通过田间小区试验,研究叶面喷施腐植酸水溶肥对冬小麦生长、产量构成因素及经济效益的影响。[方法]以徐麦35为材料,采用叶面喷施水溶肥的方式,在小麦返青期、拔节期和孕穗期各喷施1次腐植酸类叶面肥,得出施用效果。[结果]腐植酸水溶肥能增加小麦的穗数、穗粒数和千粒重,增产率达10.58%,增产效果显著,产投比为5.76∶1,经济效益明显。[结论]安徽华润公司生产的含腐植酸叶面肥可以在凤阳县大面积推广应用。

腐植酸水溶肥对枇杷幼苗生长的影响

为研究叶面喷施腐植酸水溶肥对枇杷[Eriobotrya japonica(Thunb.)Lindl.]幼苗生长的影响,以大五星枇杷幼苗为材料,叶面喷施不同稀释浓度(700、1 000、1 300、1 600倍)的腐植酸水溶肥,以喷施等量清水为对照(CK),测定腐植酸水溶肥对枇杷幼苗生物量、光合色素含量和抗氧化酶活性的影响。结果表明,叶面喷施不同浓度的腐植酸水溶肥提高了枇杷幼苗茎杆和叶片生物量、光合色素含量,且均以稀释1 000倍效果最好。稀释1 000、1 300倍腐植酸水溶肥处理提高了枇杷幼苗过氧化氢酶(CAT)、过氧化物酶(POD)和超氧化物歧化酶(SOD)活性。枇杷幼苗茎杆、叶片生物量与叶绿素a、叶绿素b、类胡萝卜素含量、CAT活性、POD活性和SOD活性呈正相关。因此,叶面喷施腐植酸水溶肥能够促进枇杷幼苗生长,且以稀释1 000倍最佳。

新型腐殖酸肥对厚皮甜瓜生长、产量及品质的影响

为探究4种新型腐殖酸肥料的施用效果,以厚皮甜瓜品种农大甜8号为供试材料,开展田间试验,研究其对甜瓜生长、产量以及品质的影响。试验结果表明,与清水对照(CK)相比,各新型肥料均在不同程度上提升了甜瓜产量,其中以HK08肥料应用效果最为显著,较CK增产22.22%。同时,该肥料可显著提高甜瓜内在营养品质并促进甜瓜植株地上部生长。综上,HK08肥料应用可有效促进甜瓜提质增收,建议进行大面积推广应用。

不同施肥组合对科尔沁沙地南缘沙地土壤健康指标的影响

为研究不同施肥组合对沙地土壤改良的效果,以辽宁省阜新市彰武县的沙地土壤为考察对象,开展了试验研究。试验以不施肥为对照(CK)处理,设置生物有机肥分别与含腐殖酸肥料(OH)、含聚谷氨酸肥料(OP)和中微量元素肥(OM)混施3个施肥处理,测定施肥后6、12、18个月的土壤中微生物数量和土壤理化性状的变化。结果表明:不同施肥处理的土壤中微生物数量均呈现细菌>放线菌>真菌的变化趋势,其中OH处理的细菌数量最多,3个取样时间的细菌数量平均值为1.510×10^(7)cfu/g,是CK处理的5.2倍;施肥处理的土壤中全磷、全钾和有机质含量均得到提高,OP处理的全钾含量平均值最高,质量分数为5.96 g/kg;施肥处理的土壤pH均呈下降趋势;施肥处理的地上部植物生物量提高。施用不同组合的肥料可以有效提高土壤微生物的数量,改善土壤理化性状,提高地上部植物生物量,是一种合理的沙地改良措施。

阳光玫瑰葡萄病毒病解决方案研究

针对阳光玫瑰葡萄病毒病常发对树体和造成果实危害严重的问题,在葡萄展叶期进行根部追肥、根部追肥+叶面喷肥处理,探究不同追肥处理及处理组合对改善病毒病病症和提升葡萄产量和品质的效果。结果表明,每666.7 m~2根部追施高氮水溶肥10 kg+腐殖酸有机水溶肥5 kg+叶喷5%氨基寡糖素水剂500倍+0.01%28高芸苔素内酯1 500倍+海藻有机水溶叶面肥1 000倍液,共3次,间隔7 d 1次的方案对新叶病毒病的控制效果最佳。与对照根施常规有机水溶肥每666.7 m~2 5 kg+平衡型水溶肥5 kg相比,新生叶片发育一直处较低水平,新生叶面积增长速度处于较高水平,显著提升葡萄的品质和产量。

复合肥配施黄腐酸微生物菌剂对小麦产量、土壤理化及微生物性质的影响

为了明确复合肥配施黄腐酸微生物菌剂对小麦产量、土壤理化性质与微生物群落的影响,于2021—2022、2022—2023年在山东东营地区开展不同施肥试验,设置不施肥(CK)、施氮磷钾复合肥(DFH)、施黄腐酸菌剂(DHF)、氮磷钾复合肥与黄腐酸菌剂配施(FHHF)4个处理,测定2年小麦产量及第二年土壤理化与微生物等指标。结果表明,连续2年FHHF处理产量均为最高,其次为DHF、DFH和CK,且不同施肥处理产量显著高于CK;不同施肥处理可有效提升土壤养分含量,其中FHHF、DFH提升效果最为明显,DHF次之;FHHF、DHF处理下土壤细菌和真菌数量、平均吸光度值和微生物呼吸强度均显著高于CK,而DFH最低,微生物多样性指标也呈现相同变化趋势。综上所述,施加黄腐酸肥料能够提高作物产量,且与氮磷钾复合肥配施时效果更好,这与其改善土壤理化性质,提高土壤微生物数量和多样性有关,在生产中可作为绿色生产技术推广。

城市污泥腐殖酸有机肥对油菜生长发育及土壤性质的影响

以漳州东墩生活污水处理厂的城市污泥为原料,利用超声辅助碱破解法提取腐殖酸,添加到鸡粪中制备污泥腐殖酸有机肥,并开展其对油菜生长发育及土壤性质的影响研究。结果表明,城市污泥腐殖酸有机肥作底肥能够提高油菜可溶性糖、VC含量,降低硝酸盐含量,从而提升油菜品质,但不会对油菜产生重金属累积危害。城市污泥腐殖酸有机肥具有较好的培肥效应,可提高土壤有机质、全磷和全氮含量,短期施用之后不会造成土壤重金属污染。本研究结果可为城市污泥腐殖酸有机肥在油菜等蔬菜种植中推广使用提供试验依据。

有机肥减量配施外源碳基肥对设施菜地氨挥发及黄瓜产量的影响

针对我国农业源氨排放过高的问题,本研究通过探究有机肥减量配施外源碳基肥(生物炭及腐植酸)对设施菜地土壤氨挥发及黄瓜产量的影响,并结合高通量qPCR基因芯片技术,研究影响氨挥发的微生物学机制,明晰设施菜地有效的氨减排措施。以天津市武清区设施菜地为研究对象,本研究共设置5个处理:不施氮CK、推荐施肥量N1(37500 kg·hm^(-2)有机肥)、推荐施肥量配施腐植酸N1F(37500kg·hm^(-2)有机肥+2250kg·hm^(-2)腐植酸)、推荐施肥量配施生物炭N1B(37500kg·hm^(-2)有机肥+40t·hm^(-2)生物炭)、农民常规用量N_(2)(60000 kg·hm^(-2)有机肥)。结果表明:不同处理氨挥发动态基本一致,N_(2)处理氨挥发速率处于较高水平。随着施肥量的增加,土壤氨挥发累积量逐渐增加,氨挥发累积量从高到低依次为N_(2)、N1、N1B、N1F、CK,N1F处理和N1B处理较N_(2)处理显著降低了24.5%和21.0%的氨挥发累积量(P<0.05)。N_(2)处理土壤在末果期铵态氮含量显著高于其他处理;N1、N1F、N1B处理在盛果期和末果期土壤硝态氮含量显著高于N_(2)处理。施肥以及配施生物炭和腐植酸均能显著增加AOA-amoA基因丰度,与CK相比,N1、N_(2)的AOA-amoA基因丰度显著提高(P<0.05);与N_(2)、N1相比,N1F和N1B处理AOA-amoA基因丰度显著提高(P<0.05)。推荐施肥量增加了土壤AOB-amoA的基因丰度,配施腐植酸和生物炭无显著影响。Mantel test+结果表明,NH4-N含量、AOA-amoA、AOB-amoA能够显著影响设施菜地土壤氨挥发。减量施肥配施生物炭和腐植酸能够在一定程度上降低设施菜地土壤的氨挥发量,且腐植酸配施效果更好,并能够有效固持土壤的无机氮。N1、N1F、N1B各处理保证了作物产量并较N_(2)处理显著提高了氮素利用率。综合考虑农学和环境效益,有机肥减量配施腐植酸N1F为适宜的施肥方式。

含腐殖酸和氨基酸水溶肥对辣椒根系、产量及果实品质的影响

为考察含腐殖酸和氨基酸水溶肥对茄果蔬菜的施用效果,以辣椒为试验对象,常规水溶肥料处理为对照,开展了大棚小区试验,测定了不同施肥处理对辣椒根系、产量及品质等指标的影响。结果表明:与对照处理相比,含腐殖酸和氨基酸水溶肥处理的辣椒根系活力、主根长、茎粗、根体积、根表面积、根尖数分别提高130.77%、23.40%、25.93%、112.12%、146.72%、97.46%;产量提高10.11%,利润增加4575.80元/hm^(2);辣椒中的维生素C、可溶性糖及辣椒素含量分别提高34.81%、77.40%、42.65%。含腐殖酸和氨基酸水溶肥对辣椒根系发育有明显的促进作用,对辣椒品质有较大的提升,能够增产增收。

含腐殖酸套餐肥对猕猴桃生长特性的影响

为考察含腐殖酸套餐肥对猕猴桃生长的影响,以施用普通套餐肥为对照,开展了田间试验。结果表明,与施用普通套餐肥相比,猕猴桃在施用含腐殖酸套餐肥后,果径、单株果数、单果质量、叶片叶绿素相对含量分别提高1.6%、8.2%、21.0%、9.3%。施用含腐殖酸套餐肥,可以促进猕猴桃的生长,提高产量。