A mathematical model for extraction of red pepper seed oil with supercritical CO2was proposed.Some factors influencing the process were investigated,including operation pressure,temperature and extraction yield Xe(%)....A mathematical model for extraction of red pepper seed oil with supercritical CO2was proposed.Some factors influencing the process were investigated,including operation pressure,temperature and extraction yield Xe(%).The model was solved by the method of weighted residuals and used to simulate the process numerically.The kinetic equation is expressed as Xe16.8606exp(t/9.98177)16.95457 and the simulation results are in excellent agreement with the experimental data.The optimal operating parameters are 30 MPa,318 K and 60 min.展开更多
Chili pepper (Capsicum spp.) fruit color is an important agronomical trait. It has been known that a large deletion in the 5' upstream region of the Ccs gene generates non-red fruit color in pepper, but the accura...Chili pepper (Capsicum spp.) fruit color is an important agronomical trait. It has been known that a large deletion in the 5' upstream region of the Ccs gene generates non-red fruit color in pepper, but the accurate size and position of the deletion and whether all the non-red cultivars had the same large deletion or not were unclarified. In this study, to identify the Ccs upstream large deletion, we carried out diagnostic PCR using six forward primers at 300 - 900 bp intervals in the 5' untranslated region of Ccs with a fixed reverse primer for a yellow fruit pepper “Sonia Gold”. Then it was revealed that 4430 bp from -3234 bp position in upstream region to 1196 bp position in exon was deleted in Ccs of “Sonia Gold”. The allele having this deletion was named ccs-del. Probably this allele is substantially the same as ccs-p1 having 4879 bp deletion reported previously. Based on the sequence determined, we developed a PCR marker to distinguish ccs-del. Genotyping of 16 cultivars of C. annuum showed that 14 had ccs-del and the remaining two had another mutant allele ccs-3. This result indicates that ccs-del is the most common allele and widely shared in non-red fruit cultivars in C. annuum. Genotyping of 16 cultivars of C. chinense clarified that one cultivar each possessed ccs-del and ccs-3. These results indicate that major alleles responsible for non-red fruit color in C. annuum were shared across species throughout interspecific introgression.展开更多
该研究基于Illumina Mi Seq高通量测序技术探讨小红尖椒对红酸汤微生物群落结构的影响,并对pH、总酸、氨基酸态氮、菌落总数和乳酸菌数进行比较分析。结果表明,与未添加小红尖椒的红酸汤(F组)相比,加小红尖椒的红酸汤(FL组)有较快的产...该研究基于Illumina Mi Seq高通量测序技术探讨小红尖椒对红酸汤微生物群落结构的影响,并对pH、总酸、氨基酸态氮、菌落总数和乳酸菌数进行比较分析。结果表明,与未添加小红尖椒的红酸汤(F组)相比,加小红尖椒的红酸汤(FL组)有较快的产酸效率和更高的氨基酸态氮含量。通过传统培养法可知,FL组菌落总数和乳酸菌数量均高于F组,FL组的乳酸菌显著高于对照组(P<0.05)。高通量测序结果表明,F组的优势菌属为乳酸杆菌属(Lactobacillus),而FL组的优势菌属为乳酸杆菌属、明串珠菌属(Leuconostoc)、魏斯氏菌属(Weissella)。Spearman相关性分析表明,F组的生物标志物与总酸呈负相关,而FL组中大部分生物标志物与总酸呈显著性正相关(P<0.05)。综上可知,添加小红尖椒的红酸汤具有更好的发酵优势,为红酸汤的产业化生产和工艺优化提供理论基础。展开更多
基金Supported by the Opening Foundation of Key Laboratory of Resource Biology and Biotechnology in Western China(ZS12015)the Ministry of Education and Natural Science Foundation of Education Committee of Shaanxi Province(11JK0598)the 2012 Xi’an Modern Agricultural Promotion Project(NC1207)
文摘A mathematical model for extraction of red pepper seed oil with supercritical CO2was proposed.Some factors influencing the process were investigated,including operation pressure,temperature and extraction yield Xe(%).The model was solved by the method of weighted residuals and used to simulate the process numerically.The kinetic equation is expressed as Xe16.8606exp(t/9.98177)16.95457 and the simulation results are in excellent agreement with the experimental data.The optimal operating parameters are 30 MPa,318 K and 60 min.
文摘Chili pepper (Capsicum spp.) fruit color is an important agronomical trait. It has been known that a large deletion in the 5' upstream region of the Ccs gene generates non-red fruit color in pepper, but the accurate size and position of the deletion and whether all the non-red cultivars had the same large deletion or not were unclarified. In this study, to identify the Ccs upstream large deletion, we carried out diagnostic PCR using six forward primers at 300 - 900 bp intervals in the 5' untranslated region of Ccs with a fixed reverse primer for a yellow fruit pepper “Sonia Gold”. Then it was revealed that 4430 bp from -3234 bp position in upstream region to 1196 bp position in exon was deleted in Ccs of “Sonia Gold”. The allele having this deletion was named ccs-del. Probably this allele is substantially the same as ccs-p1 having 4879 bp deletion reported previously. Based on the sequence determined, we developed a PCR marker to distinguish ccs-del. Genotyping of 16 cultivars of C. annuum showed that 14 had ccs-del and the remaining two had another mutant allele ccs-3. This result indicates that ccs-del is the most common allele and widely shared in non-red fruit cultivars in C. annuum. Genotyping of 16 cultivars of C. chinense clarified that one cultivar each possessed ccs-del and ccs-3. These results indicate that major alleles responsible for non-red fruit color in C. annuum were shared across species throughout interspecific introgression.
文摘该研究基于Illumina Mi Seq高通量测序技术探讨小红尖椒对红酸汤微生物群落结构的影响,并对pH、总酸、氨基酸态氮、菌落总数和乳酸菌数进行比较分析。结果表明,与未添加小红尖椒的红酸汤(F组)相比,加小红尖椒的红酸汤(FL组)有较快的产酸效率和更高的氨基酸态氮含量。通过传统培养法可知,FL组菌落总数和乳酸菌数量均高于F组,FL组的乳酸菌显著高于对照组(P<0.05)。高通量测序结果表明,F组的优势菌属为乳酸杆菌属(Lactobacillus),而FL组的优势菌属为乳酸杆菌属、明串珠菌属(Leuconostoc)、魏斯氏菌属(Weissella)。Spearman相关性分析表明,F组的生物标志物与总酸呈负相关,而FL组中大部分生物标志物与总酸呈显著性正相关(P<0.05)。综上可知,添加小红尖椒的红酸汤具有更好的发酵优势,为红酸汤的产业化生产和工艺优化提供理论基础。
