The distribution of rDNA was visualized in interphase nuclei of tumor promoter treated human lymphocytes in comparison with the mltogen Phytoheamagglutinin (PHA) effects by using an in situ hybridization fluorescent m...The distribution of rDNA was visualized in interphase nuclei of tumor promoter treated human lymphocytes in comparison with the mltogen Phytoheamagglutinin (PHA) effects by using an in situ hybridization fluorescent method. The procedure involves biotinylated rDNA as the probe and FITC-avidin as detection system. Silver (Ag) staining was used to visualize nucleoli. In the interphase nuclei of most of the nonstimulated control lymphocytes, only one small Ag-stained nucleolus could be seen. The in situ hybridization, however, revealed one to several agglomerations of rDNA fluorescent spots. With tumor promoting herb extract WCE (40 μg/ml) or TPA (60 ng/ ml) treatment, the Interphase nucleoli increased slightly in number with the morphology alteration into larger, reticular or compact granular types. Ag-stained particles also increased in number. The number of the in situ hybridization rDNA fluorescent spots and dots increased markedly and largereticulate formations of numerous rDNA spots were seen. This phenomenum resembles to the changes in PHA stimulated lymphocytes. Statistic analysized data showed signifcant difference between control and drug- treated cells. These results indicate that transcriptionally activated rDNA and amplification of total rDNA was induced by the used tumor promoters.展开更多
Nonlinear silicon photonics has shown an ability to generate, compact chip at a potential low cost. There are still barriers limitations. In this review, hybrid structures with some manipulate, and detect optical sign...Nonlinear silicon photonics has shown an ability to generate, compact chip at a potential low cost. There are still barriers limitations. In this review, hybrid structures with some manipulate, and detect optical signals on an ultrahindering its development due to essential material specific materials developed for nonlinear silicon photonics are discussed. The combination of silicon and the nonlinear materials takes advantage of both materials, which shows great potential to improve the performance and expand the applications for nonlinear silicon photonics.展开更多
THE physiological function of tPA and PAI-1 in rat and monkey ovulation has been previously documented. In order to elucidate the function of tPA and PAI-1 in spermatogenesis, we have studied expression of tPA and PAI...THE physiological function of tPA and PAI-1 in rat and monkey ovulation has been previously documented. In order to elucidate the function of tPA and PAI-1 in spermatogenesis, we have studied expression of tPA and PAI-1mRNA in rat testis by in situ hybridization,展开更多
文摘The distribution of rDNA was visualized in interphase nuclei of tumor promoter treated human lymphocytes in comparison with the mltogen Phytoheamagglutinin (PHA) effects by using an in situ hybridization fluorescent method. The procedure involves biotinylated rDNA as the probe and FITC-avidin as detection system. Silver (Ag) staining was used to visualize nucleoli. In the interphase nuclei of most of the nonstimulated control lymphocytes, only one small Ag-stained nucleolus could be seen. The in situ hybridization, however, revealed one to several agglomerations of rDNA fluorescent spots. With tumor promoting herb extract WCE (40 μg/ml) or TPA (60 ng/ ml) treatment, the Interphase nucleoli increased slightly in number with the morphology alteration into larger, reticular or compact granular types. Ag-stained particles also increased in number. The number of the in situ hybridization rDNA fluorescent spots and dots increased markedly and largereticulate formations of numerous rDNA spots were seen. This phenomenum resembles to the changes in PHA stimulated lymphocytes. Statistic analysized data showed signifcant difference between control and drug- treated cells. These results indicate that transcriptionally activated rDNA and amplification of total rDNA was induced by the used tumor promoters.
基金National Natural Science Foundation of China(NSFC)(11374263,61422510,61431166001,61725503)Natural Science Foundation of Zhejiang Province(Z18F050002)National Major Research and Development Program(2016YFB0402502)
文摘Nonlinear silicon photonics has shown an ability to generate, compact chip at a potential low cost. There are still barriers limitations. In this review, hybrid structures with some manipulate, and detect optical signals on an ultrahindering its development due to essential material specific materials developed for nonlinear silicon photonics are discussed. The combination of silicon and the nonlinear materials takes advantage of both materials, which shows great potential to improve the performance and expand the applications for nonlinear silicon photonics.
文摘THE physiological function of tPA and PAI-1 in rat and monkey ovulation has been previously documented. In order to elucidate the function of tPA and PAI-1 in spermatogenesis, we have studied expression of tPA and PAI-1mRNA in rat testis by in situ hybridization,
