A synthetic hybrid 45-peptide gene of Plasmodium falciparum (Pf), which encoded two CSP repeated peptides NANP and three merozoite peptides SPf83. 1, SPf55. 1 and SPf35. 1, was cloned in an expression vector pWR450-1,...A synthetic hybrid 45-peptide gene of Plasmodium falciparum (Pf), which encoded two CSP repeated peptides NANP and three merozoite peptides SPf83. 1, SPf55. 1 and SPf35. 1, was cloned in an expression vector pWR450-1, then the recombinant plasmid pWRA was introduced into the attenuated Salmonella typhimurium SL3261. When used as a live vaccine and administered orally (po), intravenously (iv) or intraperitoneally (ip),the recombinant strain was able to live in vivo and elicit specific humoral and cellular immunity in BALB/c mice and rabbits. As oral immunization is safe and effective, it is thought that the live recombinant Salmonella tyPhimurium vaccine may bring the Pf oral live vaccine a step nearer.展开更多
将生长抑素(ss)与乙肝表面抗原(HBsAg)融合基因(ss/HBs)克隆到痘苗病毒表达质粒pGJP-5中,通过与痘苗病毒天坛株体内重组和蚀斑挑选技术获得融合基因的重组病毒,它保持痘苗病毒原有的感染性,并能表达出 ss 和 HBsAg 产物.表达产物呈表面...将生长抑素(ss)与乙肝表面抗原(HBsAg)融合基因(ss/HBs)克隆到痘苗病毒表达质粒pGJP-5中,通过与痘苗病毒天坛株体内重组和蚀斑挑选技术获得融合基因的重组病毒,它保持痘苗病毒原有的感染性,并能表达出 ss 和 HBsAg 产物.表达产物呈表面带 ss 决定簇的 HBsAg 杂合颗粒,并分泌到感染细胞之培养液中.杂合颗粒的大小和密度与报道的 HBsAg 颗粒相似。本文还对该重组痘苗病毒可能作为 ss 活载体苗的前景进行了讨论。展开更多
文摘A synthetic hybrid 45-peptide gene of Plasmodium falciparum (Pf), which encoded two CSP repeated peptides NANP and three merozoite peptides SPf83. 1, SPf55. 1 and SPf35. 1, was cloned in an expression vector pWR450-1, then the recombinant plasmid pWRA was introduced into the attenuated Salmonella typhimurium SL3261. When used as a live vaccine and administered orally (po), intravenously (iv) or intraperitoneally (ip),the recombinant strain was able to live in vivo and elicit specific humoral and cellular immunity in BALB/c mice and rabbits. As oral immunization is safe and effective, it is thought that the live recombinant Salmonella tyPhimurium vaccine may bring the Pf oral live vaccine a step nearer.
文摘将生长抑素(ss)与乙肝表面抗原(HBsAg)融合基因(ss/HBs)克隆到痘苗病毒表达质粒pGJP-5中,通过与痘苗病毒天坛株体内重组和蚀斑挑选技术获得融合基因的重组病毒,它保持痘苗病毒原有的感染性,并能表达出 ss 和 HBsAg 产物.表达产物呈表面带 ss 决定簇的 HBsAg 杂合颗粒,并分泌到感染细胞之培养液中.杂合颗粒的大小和密度与报道的 HBsAg 颗粒相似。本文还对该重组痘苗病毒可能作为 ss 活载体苗的前景进行了讨论。
