Optimization of Hybrid Combination and an Application Example for Strawberry Breeding

This study was conducted to select new strawberry cultivars with early maturity, disease resistance and high quality, by optimizing the hybrid combinations of Benihoppe, a Japanese strawberry cultivar, which is extensively planted but not resistant to anthrax. The reciprocal crosses were performed using Benihoppe, as the female parent, four disease-resistant European cultivars Albion, Camino Real, Ven- tana and Virginia, and one Japanese cultivar Meiho as the male parent. Then, the seedling propagation coefficient, disease resistance, fruit quality and yield of F1 gen- eration of the combinations were detected and compared. Besides, the breeding and traits of a new cultivar F1-40 developed from the cross between Benihoppe and Mei- ho were introduced as an instance to prove the feasibility of the combinations/lines.

Comparative Study on Growing-finishing Performance and Meat Quality of Different Hybrid Combinations in Pigs

[Objective] The aim of this study was to investigate the production performance of three pig cultivars through their fattening performance, carcass traits and meat quality for facilitating its application in practical production. [ Method] Three pig cultivars were selected including Yesong （ F1 hybrid of pure-bred wild boar cross with songliao black sow as female parent） hybrid pigs, D × L × Y pigs from three-way cross and L x Y pigs from two-way cross. The test pigs in early period （before 60 kg） and late period （after 60 kg） were all fed with diets of the same nutritional level by free feeding and drinking water. The pre-test period was 3 d, while the test period was 120 d. Every 30 days, pigs with an empty stomach were weighed on morning, and when fattening to 120 d, five pigs with good growth and development in each cultivar were selected for slaughter to determine carcass traits and meat quality. [ Result] Under the same diets and feeding conditions, average daily gain and weight gain in the whole period of pigs from three-way cross were all significantly higher than those of pigs from two-way cross, and also extremely higher than those of Yesong pigs. There was no difference in dressed carcass percentage, backfat thickness and proportion of hind leg among three hybrid combinations. Lean meat percentage and eye muscle area of pigs from three-way cross and pigs from two-way cross were all significantly higher than those of Yesong pigs. Yesong pigs had relatively darker carnation, but marble vein （P〈0.05） and water loss rate （P〈0. 01 ） of Yesong pigs were relatively fewer than those of pigs from two-way cross and pigs from three-way cross. Shear force of Yesong pigs was significantly higher than that of pigs from two-way cross and pigs from three-way cross, [ Conclusion] Yesong hybrid pigs make full use of heterosis between wild pigs and Songliao black pigs for improvement of its meat quality, but some values of major economic traits in Yesong hybrid pigs is still lower than those in pigs from thrae-way cross and pigs from two-way cress.

Screening of Bitter Gourd Hybrid Combinations with High Yield and Disease Resistance

[Objectives]This study was conducted to breed and promote high-yield and disease-resistant varieties of bitter gourd.[Methods]The comparison experiments of six new hybrid combinations(Z1705,Z1706,Z1717,Z1721,Z1732,Z1745)were successively carried out at two experimental sites of Danzhou and Tunchang.[Results]Comparing the agronomic traits and disease resistance of various combinations,it was concluded that Z1717 and Z1721 have high yields,green and glossy fruit surface and good comprehensive disease resistance,so they can be popularized and planted.[Conclusions]This study is of great significance for improving the yield of bitter gourd and increasing economic benefits.

Advances in Breeding of High-amylose Indica Hybrid Rice Combinations in Sichuan Province

During 2000-2013, totally ten high-amylose specific hybrid rice combinations approved and five combinations for production test were bred in Sichuan Province. In the paper, rice yield, economic traits, quality indicators and rice blast-resistance identification results of these 15 hybrid combinations were described and analyzed. Results show that Chuanxiangyou37 and Tianlongyou540 have relatively high amylose content, good yielding ability, high resistance and appropriate growth period, which are new hybrid rice varieties with organic combination of high amylase content, high gel consistency, high yield, stable yield and wide adaptability. Therefore, Chuanxiangyou37 and Tianlongyou540 are recommended as the main combination for rice production.

Prediction of the Best Three-Breed Hybridized Combination of Imported Meat Sheep Using Genetic Polymorphism of Microsatellite DNA

The allelic frequency, the polymorphic information contents （PIC）, the number of effective alleles, the heterozygosity, and the genetic distances were studied in three imported meat sheep （Suffolk, Dorset, Texel） and their F1 crossbred obtained from those crossed with indigenous Small Tail Hun Sheep （Suffolk♂× Small Tail Hun Sheep, SH; Dorset ♂× Small Tail Han Sheep♂, DH; Texel♂× Small Tail Hart Sheep ♀, TH） using six microsatellite DNA loci. The perpormences of three-breed crossbred （Suffolk ♂× DH ♀, Suffolk ♂× TH ♀, Texel ♂× SH ♀, Texel ♂× DH ♀, Dorset ♂× TH ♀, and Dorset ♂× SH ♀ ） were tested. The results indicated that there were genetic polymorphisms at six microsatellite loci in six sheep populations. Six microsatellite loci could be used for genetic diversity evaluation in sheep populations. The order of three-breed heterosis by the analysis of genetic relationship from large to small was Texel ♂× DH ♀, Suffolk ♂× DH ♀, Suffolki ♂× TH ♀, Texel ♂× SH ♀, Dorset ♂×TH ♀, and Dorset ♂× SH ♀, which was in accordance with the testing results on actual heterosis. These results showed that prediction of the best three-breed hybridized combination among sheep breeds by microsatellite DNA polymorphism was feasible, which will have an important value on the reasonable utilization of introduced meat sheep and sheep breeding in our country in the future.

New hybrid rice combination—Xieyou 46

Hybrid rice combination Xieyou 46 was first bred by combining CMS line Xieqingzao A with restorer line Milyang 46 at CNRRI in 1983. It can be used as single season or late season new indica hybrid rice combination. Having passed provincial level yield trials in the following years, it was released in 1990 with the permission of Zhejiang Provincial Government, later on recommended as one of the top commercial hybrid rice combination by China State Science and Technology Commission. By the end of 1992, Xieyou 46 has been planted as a late season or single season hybrid ricc combination along the middle and lower reaches of the Yangtze River, and the planting area has reached 3.35 million ha. It has become one of the most important late season hybrid rice combinations in Zhejiang Province. Characteristics of Xieyou 46: Xieyou 46 has a suitable growth duration of 125-130 d as late season rice or 127-134 d as single season rice, 2-3 d shorter than those of Shanyou 6. It bas a compact plant type, high tilleri

Shan-You 10-A New Combination of Hybrid Rice

Hybrid rice Shan-You 10 was first madein 1982 by combining Zhen-Shan 97A,a wildcytoplasmic male sterile line used widely inChina,with Milyang 46 introduced from IRRIin 1981.In the following years,this combi-nation was tested and passed through differentformal tests including yield potential tests,

Identification and Cloning of Differentially Expressed Genes Involved in the Interaction Between Potato and Phytophthora infestans using a Subtractive Hybridization and cDNA-AFLP Combinational Approach

Using a subtractive hybridization （SH）/cDNA-AFLP combinational approach, differentially expressed genes involved in the potato-Phytophthora infestans interaction were identified. These included genes potentially controlling pathogenesis or avr genes in P. infestans as well as those potentially involved in potato resistance or susceptibility to this pathogen. Forty-one differentially expressed transcript, derived fragments （TDFs）, resulting from the interaction, were cloned and sequenced. Two TDFs, suggested as potential pathogenicity factors, have sequence similarity to N-succinyl diaminopimelate aminotransferase and a transcriptional regulator, TetR family gene, respectively. Two other TDFs, suggested as potential avr genes, have sequence similarity to an EST sequence from Avr41Cf.41Avr91Cf- 9 and a P. infestans avirulence-associated gene, respectively. Genes＇ expression and origin were confirmed using Southern blots, Northern blots and qRT-PCR, he., potential resistance gene DL81 was induced at 12 hpi in the moderately resistant cultivar, whereas it was down-regulated as early as 6 hpi in the susceptible cultivar. On the other hand, DL21 was induced at 6 hpi （3.38-fold） in response to the highly aggressive isolate （US8） and strongly up-regulated thereafter （25.13-fold at 120 hpi.）, whereas it was only slightly up-regulated in response to the weakly aggressive isolate US11 （3.82-fold at 96 hpi）, suggesting its potential involvement as a susceptibility gene.

A COMBINED HYBRID FINITE ELEMENT METHOD FOR PLATE BENDING PROBLEMS

In this paper, a combined hybrid method is applied to finite element discretization of plate bending problems. It is shown that the resultant schemes are stabilized, i.e., the convergence of the schemes is independent of inf-sup conditions and any other patch test. Based on this, two new series of plate elements are proposed.

Multiderivative Combined Dissipative Compact Scheme Satisfying Geometric Conservation Law III: Characteristic-Wise Hybrid Method

Based on newly developed weight-based smoothness detectors and non-linear interpolations designed to capture discontinuities for the multiderivative com-bined dissipative compact scheme(MDCS),hybrid linear and nonlinear interpolations are proposed to form hybrid MDCS.These detectors are derived from the weights used for the nonlinear interpolations and can provide suitable switches between the linear and the nonlinear schemes to realize the characteristics for the hybrid MDCS of capturing discontinuities and maintaining high resolution in the region without large discontinuities.To save computational cost,the nonlinear scheme with characteris-tic decomposition is only applied in the detected discontinuities region by specially designed hybrid strategy.Typical tests show that the hybrid MDCS is capable of cap-turing discontinuities and maintaining high resolution power for the smooth region at the same time.With the satisfaction of the geometric conservative law(GCL),the MDCS is further applied on curvilinear mesh to present its promising capability of handling pragmatic simulations.

Hybrid optical wavelength demultiplexer and power combiner for TWDM PON

In this paper,we have proposed a hybrid optical wavelength demultiplexer and power combiner for a hybrid timeand wavelength-division multiplexing(TWDM)passive optical network(PON),i.e.,a single passive optical device that functions as a 1×N wavelength demultiplexer for distributing the downstream signal in multiple wavelengths from the optical line terminal(OLT)to the N optical network units(ONUs),and simultaneously as an N×1 power combiner for collecting the upstream signal in the same wavelength from the N ONUs to the OLT.Through a design example of a 32 channel hybrid optical wavelength demultiplexer and power combiner on the silicon-on-insulator platform,our numerical simulation result shows that the insertion loss and adjacent channel crosstalk of the downstream wavelength demultiplexer are as low as 4.6 and-16.3 dB,respectively,while the insertion loss and channel non-uniformity of the upstream power combiner can reach 3.5 and 2.1 dB,respectively.The proposed structure can readily be extended to other material platforms such as the silica-based planar lightwave circuit.Its fabrication process is fully compatible with standard clean-room technologies such as photolithography and etching,without any complicated and/or costly approach involved.