Ping′ou hybrid hazelnut is produced by cross cultivation and is widely cultivated in northern China with good development prospects.Based on a field experiment of fertilizer efficiency,the leaf spectral reflectance a...Ping′ou hybrid hazelnut is produced by cross cultivation and is widely cultivated in northern China with good development prospects.Based on a field experiment of fertilizer efficiency,the leaf spectral reflectance and leaf potassium(K)concentration were measured with different quantities of K fertilizer applied at four fruit growth stages(fruit setting stage,fruit rapid growth stage,fruit fat-change stage,and fruit near-maturity stage)of Ping′ou hybrid hazelnut in 2019.Spectral parameters that were significantly correlated with leaf K concentration were selected using Pearson correlation analysis,and spectral parameter estimation models of leaf K concentration were established by employing six different modelling methods(exponential function,power function,logarithmic function,linear function,quadratic function,and cubic function).The results indicated that at the fruit setting period,leaf K concentration was significantly correlated with Dy(spectra slope of yellow edge),Rg(reflectance of the green peak position),λo(red valley position),SDb(blue edge area),SDr/SDb(where SDr represents red edge area),and(SDr–SDb)/(SDr+SDb)(P<0.01).There were significant correlations of leaf K concentration with Dy,Rg,SDb,Rg/Ro(where Ro is the reflectance of the red valley position),and(Rg–Ro)/(Rg+Ro)at the fruit rapid growth stage(P<0.01).Further,significant correlations of leaf K concentration with Rg,Ro,RNIR/Green,and RNIR/Blue were obtained at the fruit fat-change period(P<0.01).Finally,leaf K concentration showed significant correlations with Dr,Rg,Ro,SDy(yellow edge area),and SDr at the fruit near-maturity stage(P<0.01).Through a cubic function analysis,regression estimation model of leaf K concentration with highest fitting degree(R2)values at the four fruit growth stages was established.The findings in this study demonstrated that it is feasible to estimate leaf K concentration of Ping′ou hybrid hazelnut at the various phenological stages of fruit development by establishing regression models between leaf K concentration and spectral parameters.展开更多
【目的】建立平欧杂种榛FISH-AFLP技术体系,并应用该技术分析平欧杂种榛主栽品种的遗传关系。【方法】以达维等10个主栽品种为试材,经DNA提取、PstⅠ/MseⅠ双酶切,进行FISH-AFLP反应,数据转换为"0—1"矩阵后,使用NTSYS pc 2....【目的】建立平欧杂种榛FISH-AFLP技术体系,并应用该技术分析平欧杂种榛主栽品种的遗传关系。【方法】以达维等10个主栽品种为试材,经DNA提取、PstⅠ/MseⅠ双酶切,进行FISH-AFLP反应,数据转换为"0—1"矩阵后,使用NTSYS pc 2.11F和Popgene 1.32软件进行数据分析并作图。【结果】从64对引物中,筛选出15对多态性强的PstⅠ/MseⅠ引物,共获得1 739条谱带,引物平均多态带比率97.94%;平欧杂种榛主栽品种的相似性系数为0.7556—0.8543,当阈值为0.8398时,可分成4个AFLP群,其中,玉坠(84-310)、辽榛4号(85-41)和平欧69号(84-69)单独为1群,其他品种为1群;平欧杂种榛主栽品种的有效等位基因数、基因多样度、Shannon信息指数分别为1.3921、0.2482和0.3957,具有较高的遗传多样性;研究获得的特征条带可用于平欧杂种榛主栽品种的快速鉴别。【结论】由于平欧杂种榛的实生选优群体是多亲本组合的混合后代,因此,该群体是一个具有高度遗传多样性的群体,主栽品种间存在复杂的亲缘关系;研究构建的FISH-AFLP技术流程、筛选得到的引物以及分析得到的各项遗传参数,可为其他榛属植物的相关研究提供参考。展开更多
基金the National Natural Science Foundation of China(31960324)。
文摘Ping′ou hybrid hazelnut is produced by cross cultivation and is widely cultivated in northern China with good development prospects.Based on a field experiment of fertilizer efficiency,the leaf spectral reflectance and leaf potassium(K)concentration were measured with different quantities of K fertilizer applied at four fruit growth stages(fruit setting stage,fruit rapid growth stage,fruit fat-change stage,and fruit near-maturity stage)of Ping′ou hybrid hazelnut in 2019.Spectral parameters that were significantly correlated with leaf K concentration were selected using Pearson correlation analysis,and spectral parameter estimation models of leaf K concentration were established by employing six different modelling methods(exponential function,power function,logarithmic function,linear function,quadratic function,and cubic function).The results indicated that at the fruit setting period,leaf K concentration was significantly correlated with Dy(spectra slope of yellow edge),Rg(reflectance of the green peak position),λo(red valley position),SDb(blue edge area),SDr/SDb(where SDr represents red edge area),and(SDr–SDb)/(SDr+SDb)(P<0.01).There were significant correlations of leaf K concentration with Dy,Rg,SDb,Rg/Ro(where Ro is the reflectance of the red valley position),and(Rg–Ro)/(Rg+Ro)at the fruit rapid growth stage(P<0.01).Further,significant correlations of leaf K concentration with Rg,Ro,RNIR/Green,and RNIR/Blue were obtained at the fruit fat-change period(P<0.01).Finally,leaf K concentration showed significant correlations with Dr,Rg,Ro,SDy(yellow edge area),and SDr at the fruit near-maturity stage(P<0.01).Through a cubic function analysis,regression estimation model of leaf K concentration with highest fitting degree(R2)values at the four fruit growth stages was established.The findings in this study demonstrated that it is feasible to estimate leaf K concentration of Ping′ou hybrid hazelnut at the various phenological stages of fruit development by establishing regression models between leaf K concentration and spectral parameters.
文摘【目的】建立平欧杂种榛FISH-AFLP技术体系,并应用该技术分析平欧杂种榛主栽品种的遗传关系。【方法】以达维等10个主栽品种为试材,经DNA提取、PstⅠ/MseⅠ双酶切,进行FISH-AFLP反应,数据转换为"0—1"矩阵后,使用NTSYS pc 2.11F和Popgene 1.32软件进行数据分析并作图。【结果】从64对引物中,筛选出15对多态性强的PstⅠ/MseⅠ引物,共获得1 739条谱带,引物平均多态带比率97.94%;平欧杂种榛主栽品种的相似性系数为0.7556—0.8543,当阈值为0.8398时,可分成4个AFLP群,其中,玉坠(84-310)、辽榛4号(85-41)和平欧69号(84-69)单独为1群,其他品种为1群;平欧杂种榛主栽品种的有效等位基因数、基因多样度、Shannon信息指数分别为1.3921、0.2482和0.3957,具有较高的遗传多样性;研究获得的特征条带可用于平欧杂种榛主栽品种的快速鉴别。【结论】由于平欧杂种榛的实生选优群体是多亲本组合的混合后代,因此,该群体是一个具有高度遗传多样性的群体,主栽品种间存在复杂的亲缘关系;研究构建的FISH-AFLP技术流程、筛选得到的引物以及分析得到的各项遗传参数,可为其他榛属植物的相关研究提供参考。
