Microalgae are photosynthetic microorganisms that function as primary producers in aquatic ecosystems. Some species of microalgae undergo rapid growth and cause harmful blooms in marine ecosystems. Heterocapsa triquet...Microalgae are photosynthetic microorganisms that function as primary producers in aquatic ecosystems. Some species of microalgae undergo rapid growth and cause harmful blooms in marine ecosystems. Heterocapsa triquetra is one of the most common bloom-forming species in estuarine and coastal waters worldwide. Although this species does not produce toxins, unlike some other Heterocapsa species, the high density of its blooms can cause significant ecological damage. We developed a H. triquetra species-specific nuclease protection assay sandwich hybridization（NPA-SH） probe that targets the large subunit of ribosomal RNA（LSU r RNA）. We tested probe specificity and sensitivity with five other dinoflagellates that also cause red tides. Our assay detected H.triquetra at a concentration of 1.5×10^4 cells/m L, more sensitive than required for a red-tide guidance warning by the Korea Ministry of Oceans and Fisheries in 2015（3.0×10^4 cells/m L）. We also used the NPA-SH assay to monitor H. triquetra in the Tongyeong region of the southern sea area of Korea during 2014. This method could detect H.triquetra cells within 3 h. Our assay is useful for monitoring H. triquetra under field conditions.展开更多
We report a fiber Bragg grating（FBG）-based sensor for the simultaneous measurement of a train bearing’s vibration and temperature. A pre-stretched optical fiber with an FBG and a mass is designed for axial vibratio...We report a fiber Bragg grating（FBG）-based sensor for the simultaneous measurement of a train bearing’s vibration and temperature. A pre-stretched optical fiber with an FBG and a mass is designed for axial vibration sensing. Another multiplexed FBG is embedded in a selected copper-based alloy with a high thermal expansion to detect temperature. Experiments show that the sensor possesses a high resonant frequency of 970 Hz, an acceleration sensitivity of 27.28 pm/g, and a high temperature sensitivity of 35.165 pm/℃. A resonant excitation test is also carried out that demonstrates the robustness and reliability of the sensor.展开更多
基金The Public Welfare&Safety Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Science,ICT&Future Planning under contract No.NRF-2013M3A2A1067529
文摘Microalgae are photosynthetic microorganisms that function as primary producers in aquatic ecosystems. Some species of microalgae undergo rapid growth and cause harmful blooms in marine ecosystems. Heterocapsa triquetra is one of the most common bloom-forming species in estuarine and coastal waters worldwide. Although this species does not produce toxins, unlike some other Heterocapsa species, the high density of its blooms can cause significant ecological damage. We developed a H. triquetra species-specific nuclease protection assay sandwich hybridization（NPA-SH） probe that targets the large subunit of ribosomal RNA（LSU r RNA）. We tested probe specificity and sensitivity with five other dinoflagellates that also cause red tides. Our assay detected H.triquetra at a concentration of 1.5×10^4 cells/m L, more sensitive than required for a red-tide guidance warning by the Korea Ministry of Oceans and Fisheries in 2015（3.0×10^4 cells/m L）. We also used the NPA-SH assay to monitor H. triquetra in the Tongyeong region of the southern sea area of Korea during 2014. This method could detect H.triquetra cells within 3 h. Our assay is useful for monitoring H. triquetra under field conditions.
基金supported in part by the National Natural Science Foundation of China(Nos.51605348 and 51605344)in part by the Natural Science Foundation of the Hubei Province(No.2016CFB116)in part by the Open Research Fund of the Hubei Digital Manufacturing Key Laboratory(No.SZ1801)
文摘We report a fiber Bragg grating（FBG）-based sensor for the simultaneous measurement of a train bearing’s vibration and temperature. A pre-stretched optical fiber with an FBG and a mass is designed for axial vibration sensing. Another multiplexed FBG is embedded in a selected copper-based alloy with a high thermal expansion to detect temperature. Experiments show that the sensor possesses a high resonant frequency of 970 Hz, an acceleration sensitivity of 27.28 pm/g, and a high temperature sensitivity of 35.165 pm/℃. A resonant excitation test is also carried out that demonstrates the robustness and reliability of the sensor.
