Objective:Circular RNAs(circRNAs)have been shown to involve in pathological processes of ischemic stroke(IS),including autophagy.This study was designed to explore the effect of circR-ZC3HC1 on neuronal autophagy in I...Objective:Circular RNAs(circRNAs)have been shown to involve in pathological processes of ischemic stroke(IS),including autophagy.This study was designed to explore the effect of circR-ZC3HC1 on neuronal autophagy in IS and the related mechanisms.Methods:Expression of circR-ZC3HC1 in blood samples of IS patients and healthy controls was detected.Hippocampal neurons were treated with oxygen and glucose deprivation(OGD)to establish IS in vitro model.The expression of LC3 and p62 and the number of autophagosomes were examined to evaluate the autophagy level of OGD induced neurons using western blotting and transmission electron microscope.Cell apoptosis rate and the expression of cleaved caspase-3,Bax,and Bcl-2 were assessed byflow cytometry and western blotting.The binding relationships among circR-ZC3HC1,miR-384-5p,and SIRT1 were predicted and verified.Results:Low expression of circR-ZC3HC1 was found in blood samples of IS patients and OGD-treated neurons.Overexpressed circR-ZC3HC1 or inhibited miR-384-5p expression promoted autophagy and inhibited apoptosis of OGD-treated neurons,which could be reversed by further 3-MA treatment.Mechanistically,circR-ZC3HC1 targeted miR-384-5p to mediate SIRT1 expression.miR-384-5p overexpression or SIRT1 knockdown in the presence of circR-ZC3HC1 overexpression in OGD-treated neurons lead to reduced autophagy and enhanced apoptosis.Conclusion:Collectively,circR-ZC3HC1 promoted neuronal autophagy to attenuate IS via miR-384-5p/SIRT1 axis.展开更多
为提升国内某1340 mm HC冷连轧机组的带钢板形质量和生产效率,建立了以带钢板形良好、电机功率平衡和预防打滑为优化目标的四机架HC冷连轧机负荷分配多目标优化模型,采用非支配排序遗传算法(NSGA-Ⅱ)获得Pareto最优解集。按轧制状态将...为提升国内某1340 mm HC冷连轧机组的带钢板形质量和生产效率,建立了以带钢板形良好、电机功率平衡和预防打滑为优化目标的四机架HC冷连轧机负荷分配多目标优化模型,采用非支配排序遗传算法(NSGA-Ⅱ)获得Pareto最优解集。按轧制状态将工作辊辊期划分为4个阶段,基于各阶段工作辊的热凸度、粗糙度以及控轧需求,合理确定3个优化目标的权重系数,解决目标函数间的冲突关系,从而获得能适应辊期动态变化的轧制规程。仿真表明:优化后的轧制规程有助于改善板形质量、提高生产效率并减小打滑概率。展开更多
基金Supported by Ningbo Health Technology Project,Nos.2020Y12 and 2022Y12.
文摘Objective:Circular RNAs(circRNAs)have been shown to involve in pathological processes of ischemic stroke(IS),including autophagy.This study was designed to explore the effect of circR-ZC3HC1 on neuronal autophagy in IS and the related mechanisms.Methods:Expression of circR-ZC3HC1 in blood samples of IS patients and healthy controls was detected.Hippocampal neurons were treated with oxygen and glucose deprivation(OGD)to establish IS in vitro model.The expression of LC3 and p62 and the number of autophagosomes were examined to evaluate the autophagy level of OGD induced neurons using western blotting and transmission electron microscope.Cell apoptosis rate and the expression of cleaved caspase-3,Bax,and Bcl-2 were assessed byflow cytometry and western blotting.The binding relationships among circR-ZC3HC1,miR-384-5p,and SIRT1 were predicted and verified.Results:Low expression of circR-ZC3HC1 was found in blood samples of IS patients and OGD-treated neurons.Overexpressed circR-ZC3HC1 or inhibited miR-384-5p expression promoted autophagy and inhibited apoptosis of OGD-treated neurons,which could be reversed by further 3-MA treatment.Mechanistically,circR-ZC3HC1 targeted miR-384-5p to mediate SIRT1 expression.miR-384-5p overexpression or SIRT1 knockdown in the presence of circR-ZC3HC1 overexpression in OGD-treated neurons lead to reduced autophagy and enhanced apoptosis.Conclusion:Collectively,circR-ZC3HC1 promoted neuronal autophagy to attenuate IS via miR-384-5p/SIRT1 axis.
文摘为提升国内某1340 mm HC冷连轧机组的带钢板形质量和生产效率,建立了以带钢板形良好、电机功率平衡和预防打滑为优化目标的四机架HC冷连轧机负荷分配多目标优化模型,采用非支配排序遗传算法(NSGA-Ⅱ)获得Pareto最优解集。按轧制状态将工作辊辊期划分为4个阶段,基于各阶段工作辊的热凸度、粗糙度以及控轧需求,合理确定3个优化目标的权重系数,解决目标函数间的冲突关系,从而获得能适应辊期动态变化的轧制规程。仿真表明:优化后的轧制规程有助于改善板形质量、提高生产效率并减小打滑概率。
