Hydrogel patch-based stem cell transplantation and microenvironment-regulating drug delivery strategy is promising for the treatment of myocardial infarction(MI).However,the low retention of cells and drugs limits the...Hydrogel patch-based stem cell transplantation and microenvironment-regulating drug delivery strategy is promising for the treatment of myocardial infarction(MI).However,the low retention of cells and drugs limits their therapeutic efficacies.Here,we propose a prefixed sponge carpet strategy,that is,aldehyde-dextran sponge(ODS)loading anti-oxidative/autophagy-regulating molecular capsules of 2-hydroxy-β-cyclodextrin@resveratrol(HP-β-CD@Res)is first bonded to the rat’s heart via capillary removal of interfacial water from the tissue surface,and the subsequent Schiff base reaction between the aldehyde groups on ODS and amino groups on myocardium tissue.Then,an aqueous biocompatible hydrazided hyaluronic acid(HHA)solution encapsulating mesenchymal stem cells(MSCs)is impregnated into the anchored carpet to form HHA@ODS@HP-β-CD@Res hydrogel in situ via click reaction,thus prolonging the in vivo retention time of therapeutic drug and cells.Importantly,the HHA added to outer surface consumes the remaining aldehydes to contribute to nonsticky top surface,avoiding adhesion to other tissues.The embedded HP-β-CD@Res molecular capsules with antioxidant and autophagy regulation bioactivities can considerably improve cardiac microenvironment,reduce cardiomyocyte apoptosis,and enhance the survival of transplanted MSCs,thereby promoting cardiac repair by facilitating angiogenesis and reducing cardiac fibrosis.展开更多
Adhesive hydrogel has drawn great attention for wide applications in wound healing owing to its excellent biocompatibility and lasting adhesiveness.However,traditional adhesive hydrogels only keep the wound moist to p...Adhesive hydrogel has drawn great attention for wide applications in wound healing owing to its excellent biocompatibility and lasting adhesiveness.However,traditional adhesive hydrogels only keep the wound moist to promote wound healing.It is still imperative to fabricate adhesive hydrogels that exhibit efficient antibacterial ability,active driving dynamic wound closure,and reactive oxygen species(ROS)scavenging together with excellent mechanical properties.Here,a novel hydrogel based on poly(N-isopropyl acrylamide)(PNIPAAm),a thermoresponsive polymer,and tannic acid(TA)-Ag nanoparticles(TA-Ag NPs)exhibiting active contraction,tissue adhesion,anti-inflammatory and antibacterial functions was developed.TA-Ag dispersed in the hydrogel not only functioned as the catalyst to polymerize the reaction but also provided additional anti-inflammatory and antibacterial properties.Besides,tannic acid containing catechol groups endowed the hydrogel with adhesive ability.More interestingly,the obtained hydrogel exhibited the thermoresponsive shrinkage ability,which could mechanically drive wound closure due to the presence of PNIPAAm network.In vivo mouse full-thickness skin defect model demonstrated that this actively contractible and antibacterial hydrogel is a promising dressing to improve wound healing process by accelerating tissue regeneration and preventing bacterial infection.Therefore,this multi-functional adhesive hydrogel developed here may provide a new possibility for wound healing.展开更多
基金National Natural Science Foundation of China(Grant No.52233008,51733006)National Key Research and Development Program(Grant No.2018YFA0703100).
文摘Hydrogel patch-based stem cell transplantation and microenvironment-regulating drug delivery strategy is promising for the treatment of myocardial infarction(MI).However,the low retention of cells and drugs limits their therapeutic efficacies.Here,we propose a prefixed sponge carpet strategy,that is,aldehyde-dextran sponge(ODS)loading anti-oxidative/autophagy-regulating molecular capsules of 2-hydroxy-β-cyclodextrin@resveratrol(HP-β-CD@Res)is first bonded to the rat’s heart via capillary removal of interfacial water from the tissue surface,and the subsequent Schiff base reaction between the aldehyde groups on ODS and amino groups on myocardium tissue.Then,an aqueous biocompatible hydrazided hyaluronic acid(HHA)solution encapsulating mesenchymal stem cells(MSCs)is impregnated into the anchored carpet to form HHA@ODS@HP-β-CD@Res hydrogel in situ via click reaction,thus prolonging the in vivo retention time of therapeutic drug and cells.Importantly,the HHA added to outer surface consumes the remaining aldehydes to contribute to nonsticky top surface,avoiding adhesion to other tissues.The embedded HP-β-CD@Res molecular capsules with antioxidant and autophagy regulation bioactivities can considerably improve cardiac microenvironment,reduce cardiomyocyte apoptosis,and enhance the survival of transplanted MSCs,thereby promoting cardiac repair by facilitating angiogenesis and reducing cardiac fibrosis.
基金supported by the National Research Programs of China(Nos.2020YFA0211100,and 2022YFA1206500)the National Natural Science Foundation of China(Nos.52250002,and 52325106)Suzhou Key Laboratory of Nanotechnology and Biomedicine,Collaborative Innovation Center of Suzhou Nano Science and Technology,and the 111 Program from the Ministry of Education of China.
文摘Adhesive hydrogel has drawn great attention for wide applications in wound healing owing to its excellent biocompatibility and lasting adhesiveness.However,traditional adhesive hydrogels only keep the wound moist to promote wound healing.It is still imperative to fabricate adhesive hydrogels that exhibit efficient antibacterial ability,active driving dynamic wound closure,and reactive oxygen species(ROS)scavenging together with excellent mechanical properties.Here,a novel hydrogel based on poly(N-isopropyl acrylamide)(PNIPAAm),a thermoresponsive polymer,and tannic acid(TA)-Ag nanoparticles(TA-Ag NPs)exhibiting active contraction,tissue adhesion,anti-inflammatory and antibacterial functions was developed.TA-Ag dispersed in the hydrogel not only functioned as the catalyst to polymerize the reaction but also provided additional anti-inflammatory and antibacterial properties.Besides,tannic acid containing catechol groups endowed the hydrogel with adhesive ability.More interestingly,the obtained hydrogel exhibited the thermoresponsive shrinkage ability,which could mechanically drive wound closure due to the presence of PNIPAAm network.In vivo mouse full-thickness skin defect model demonstrated that this actively contractible and antibacterial hydrogel is a promising dressing to improve wound healing process by accelerating tissue regeneration and preventing bacterial infection.Therefore,this multi-functional adhesive hydrogel developed here may provide a new possibility for wound healing.
