The functional recovery of peripheral nerve injury(PNI)is unsatisfactory,whereas diabetes mellitus(DM)and its related complications further attenuate the restoration of diabetic PNI(DPNI).Adipose-derived stem cells(AD...The functional recovery of peripheral nerve injury(PNI)is unsatisfactory,whereas diabetes mellitus(DM)and its related complications further attenuate the restoration of diabetic PNI(DPNI).Adipose-derived stem cells(ADSCs)are promising candidates for treatment of DPNI due to their abundant source,excellent differentiation and paracrine ability.Our results showed that ADSCs remarkably enhanced the proliferation and migration of Schwann cells and endothelial cells,and tube formation.Mechanistically,ADSCs could regulate Nrf2/HO-1,NF-κB and PI3K/AKT/mTOR signaling pathways,showing multiple functions in reducing oxidative stress and inflammation,and regulating cell metabolism,growth,survival,proliferation,angiogenesis,differentiation of Schwann cell and myelin formation.In current study,novel graphene foam(GF)/hydrogel-based scaffold was developed to deliver ADSCs for treatment of DPNI.GF/hydrogel scaffold exhibited excellent mechanical strength,suitable porous network,superior electrical conductivity,and good biocompatibility.In vitro results revealed that GF/hydrogel scaffold could obviously accelerate proliferation of Schwann cells.Moreover,in vivo experiments demonstrated that ADSCs-loaded GF/hydrogel scaffold significantly promoted the recovery of DPNI and inhibited the atrophy of targeted muscles,thus providing a novel and attractive therapeutic approach for DPNI patients.展开更多
At present there is a growing need for tissue engineering products,including the products of scaffold-technologies.Biopolymer hydrogel scaffolds have a number of advantages and are increasingly being used to provide m...At present there is a growing need for tissue engineering products,including the products of scaffold-technologies.Biopolymer hydrogel scaffolds have a number of advantages and are increasingly being used to provide means of cell transfer for therapeutic treatments and for inducing tissue regeneration.This work presents original hydrogel biopolymer scaffolds based on a blood plasma cryoprecipitate and collagen and formed under conditions of enzymatic hydrolysis.Two differently originated collagens were used for the scaffold formation.During this work the structural and mechanical characteristics of the scaffold were studied.It was found that,depending on the origin of collagen,scaffolds possess differences in their structural and mechanical characteristics.Both types of hydrogel scaffolds have good biocompatibility and provide conditions that maintain the three-dimensional growth of adipose tissue stem cells.Hence,scaffolds based on such a blood plasma cryoprecipitate and collagen have good prospects as cell carriers and can be widely used in regenerative medicine.展开更多
Spinal cord injury(SCI)is a serious traumatic disease of the central nervous system,which can give rise to the loss of motor and sensory function.Due to its complex pathological mechanism,the treatment of this disease...Spinal cord injury(SCI)is a serious traumatic disease of the central nervous system,which can give rise to the loss of motor and sensory function.Due to its complex pathological mechanism,the treatment of this disease still faces a huge challenge.Hydrogels with good biocompatibility and biodegradability can well imitate the extracellular matrix in the microenvironment of spinal cord.Hydrogels have been regarded as promising SCI repair material in recent years and continuous studies have confirmed that hydrogel-based therapy can effectively eliminate inflammation and promote spinal cord repair and regeneration to improve SCI.In this review,hydrogel-based multimodal therapeutic strategies to repair SCI are provided,and a combination of hydrogel scaffolds and other therapeutic modalities are discussed,with particular emphasis on the repair mechanism of SCI.展开更多
A variety of skin substitutes are used in the treatment of full-thickness burns. Substitutes made from skin can harbor latent viruses, and artificial skin grafts can heal with extensive scarring, failing to regenerate...A variety of skin substitutes are used in the treatment of full-thickness burns. Substitutes made from skin can harbor latent viruses, and artificial skin grafts can heal with extensive scarring, failing to regenerate structures such as glands, nerves, and hair follicles. Biodegradable and biocompatible hydrogels, however, rarely mimic the strength of the epidermis. Therefore, novel and practical skin scaffold materials remain to be developed. Polysaccharides form hydrogels with predicted inherent biocompatibility. This paper describes the preparation and biocompatibility of unique hydrogel skin scaffolds from plant-extracted polysaccharide mixtures of specific sources, types, and molecular weight fractions. These hydrogels have a range of mechanical and degradation properties with the potential to fulfill the multiple, diverse functions of artificial skin, including protection, compatibility with different cell types, biodegradation, and release of needed signals for cell growth and wound healing.展开更多
Tissue engineering technologies offer new treatment strategies for the repair of peripheral nerve injury, hut cell loss between seeding and adhesion to the scaffold remains inevitable. A thermosensitive collagen hydro...Tissue engineering technologies offer new treatment strategies for the repair of peripheral nerve injury, hut cell loss between seeding and adhesion to the scaffold remains inevitable. A thermosensitive collagen hydrogel was used as an extracellular matrix in this study and combined with bone marrow mesenchymal stem cells to construct tissue-engineered peripheral nerve composites in vitro. Dynamic culture was performed at an oscillating frequency of 0.5 Hz and 35° swing angle above and below the horizontal plane. The results demonstrated that bone marrow mesenchymal stem cells formed membrane-like structures around the poly-L-lactic acid scaffolds and exhibited regular alignment on the composite surface. Collagen was used to fill in the pores, and seeded cells adhered onto the poly-L-lactic acid fibers. The DNA content of the bone marrow mesenchymal stem cells was higher in the composites constructed with a thermosensitive collagen hydrogel compared with that in collagen I scaffold controls. The cellular DNA content was also higher in the thermosensitive collagen hydrogel composites constructed with the thermosensitive collagen hydrogel in dynamic culture than that in static culture. These results indicate that tissue-engineered composites formed with thermosensitive collagen hydrogel in dynamic culture can maintain larger numbers of seeded cells by avoiding cell loss during the initial adhe-sion stage. Moreover, seeded cells were distributed throughout the material.展开更多
背景:由于关节软骨的解剖、生理特点,其自我修复能力有限,故而如何修复大面积的软骨缺损(直径>4 mm)成为医学界备受瞩目的问题之一。伴随生物材料和组织工程学科的发展,通过支架技术尤其具有模拟细胞外基质微环境的水凝胶支架研究的...背景:由于关节软骨的解剖、生理特点,其自我修复能力有限,故而如何修复大面积的软骨缺损(直径>4 mm)成为医学界备受瞩目的问题之一。伴随生物材料和组织工程学科的发展,通过支架技术尤其具有模拟细胞外基质微环境的水凝胶支架研究的深入发展,为软骨损伤修复提供了新的治疗思路。目的:就海藻酸盐的性质、海藻酸盐水凝胶支架的制备以及在软骨损伤修复中的研究进展进行综述。方法:利用计算机检索Web of Science、PubMed、万方和中国知网数据库,中文检索词为“海藻酸盐水凝胶、软骨组织工程或软骨、骨软骨、支架”,英文检索词为“alginate hydrogel,cartilage tissue engineering or chondro*,osteochondral,scaffold”,检索文献时间范围2016年1月至2020年12月。最终按入组标准筛选后纳入60篇文献进行综述分析。结果与结论:①海藻酸盐作为带负电荷的天然亲水性多糖,可通过修饰技术赋予传统海藻酸盐水凝胶更优良的机械性能、黏附性、生物降解性以及生物相容性等。②修饰后的海藻酸盐复合水凝胶支架利于维持种子细胞的正常形态、合成相应的细胞外基质,促进成软骨相关基因的表达,表现出优良的成软骨能力。③有报道证实海藻酸盐复合水凝胶支架可在动物体内形成与周边正常软骨相似的软骨组织,有效修复缺损部位。④因此,海藻酸盐复合水凝胶支架具备良好的促软骨修复能力,为软骨组织工程提供新的治疗思路,但该材料未来还需更完善的临床前试验数据的支撑,以推进其临床转化进程。展开更多
基金This study is financially supported by the National Natural Science Foundation of China(Nos.81971758,51890892,81971712,81870346,and 81700432)the Natural Science Foundation of Shanghai Science and Technology Committee(No.20ZR1431600)+7 种基金This research is also supported by the National Natural Science Foundation of China(No.11761161004)Z.L.acknowledge supports by the National Natural Science Foundation of China-Research Grants Council Joint Research Scheme(Nos.11761161004 and N_HKUST607/17)the IER foundation(No.HT-JD-CXY-201907)“International science and technology cooperation projects”of Science and Technological Bureau of Guangzhou Huangpu District(No.2019GH06)Guangdong Science and Technology Department(No.2020A0505090003)Research Fund of Guangdong-Hong Kong-Macao Joint Laboratory for Intelligent Micro-Nano Optoelectronic Technology(No.2020B1212030010)Technical assistance from the Materials Characterization and Preparation Facilities of The Hong Kong University Of Science And Technology is greatly appreciatedWe also acknowledge the support of Guangdong Provincial Key Laboratory Program(No.2021B1212040001)from the Department of Science and Technology of Guangdong Province.
文摘The functional recovery of peripheral nerve injury(PNI)is unsatisfactory,whereas diabetes mellitus(DM)and its related complications further attenuate the restoration of diabetic PNI(DPNI).Adipose-derived stem cells(ADSCs)are promising candidates for treatment of DPNI due to their abundant source,excellent differentiation and paracrine ability.Our results showed that ADSCs remarkably enhanced the proliferation and migration of Schwann cells and endothelial cells,and tube formation.Mechanistically,ADSCs could regulate Nrf2/HO-1,NF-κB and PI3K/AKT/mTOR signaling pathways,showing multiple functions in reducing oxidative stress and inflammation,and regulating cell metabolism,growth,survival,proliferation,angiogenesis,differentiation of Schwann cell and myelin formation.In current study,novel graphene foam(GF)/hydrogel-based scaffold was developed to deliver ADSCs for treatment of DPNI.GF/hydrogel scaffold exhibited excellent mechanical strength,suitable porous network,superior electrical conductivity,and good biocompatibility.In vitro results revealed that GF/hydrogel scaffold could obviously accelerate proliferation of Schwann cells.Moreover,in vivo experiments demonstrated that ADSCs-loaded GF/hydrogel scaffold significantly promoted the recovery of DPNI and inhibited the atrophy of targeted muscles,thus providing a novel and attractive therapeutic approach for DPNI patients.
文摘At present there is a growing need for tissue engineering products,including the products of scaffold-technologies.Biopolymer hydrogel scaffolds have a number of advantages and are increasingly being used to provide means of cell transfer for therapeutic treatments and for inducing tissue regeneration.This work presents original hydrogel biopolymer scaffolds based on a blood plasma cryoprecipitate and collagen and formed under conditions of enzymatic hydrolysis.Two differently originated collagens were used for the scaffold formation.During this work the structural and mechanical characteristics of the scaffold were studied.It was found that,depending on the origin of collagen,scaffolds possess differences in their structural and mechanical characteristics.Both types of hydrogel scaffolds have good biocompatibility and provide conditions that maintain the three-dimensional growth of adipose tissue stem cells.Hence,scaffolds based on such a blood plasma cryoprecipitate and collagen have good prospects as cell carriers and can be widely used in regenerative medicine.
文摘Spinal cord injury(SCI)is a serious traumatic disease of the central nervous system,which can give rise to the loss of motor and sensory function.Due to its complex pathological mechanism,the treatment of this disease still faces a huge challenge.Hydrogels with good biocompatibility and biodegradability can well imitate the extracellular matrix in the microenvironment of spinal cord.Hydrogels have been regarded as promising SCI repair material in recent years and continuous studies have confirmed that hydrogel-based therapy can effectively eliminate inflammation and promote spinal cord repair and regeneration to improve SCI.In this review,hydrogel-based multimodal therapeutic strategies to repair SCI are provided,and a combination of hydrogel scaffolds and other therapeutic modalities are discussed,with particular emphasis on the repair mechanism of SCI.
文摘A variety of skin substitutes are used in the treatment of full-thickness burns. Substitutes made from skin can harbor latent viruses, and artificial skin grafts can heal with extensive scarring, failing to regenerate structures such as glands, nerves, and hair follicles. Biodegradable and biocompatible hydrogels, however, rarely mimic the strength of the epidermis. Therefore, novel and practical skin scaffold materials remain to be developed. Polysaccharides form hydrogels with predicted inherent biocompatibility. This paper describes the preparation and biocompatibility of unique hydrogel skin scaffolds from plant-extracted polysaccharide mixtures of specific sources, types, and molecular weight fractions. These hydrogels have a range of mechanical and degradation properties with the potential to fulfill the multiple, diverse functions of artificial skin, including protection, compatibility with different cell types, biodegradation, and release of needed signals for cell growth and wound healing.
基金supported by the National Natural Science Foundation of China,No.31071222Jilin Province Science and Technology Development Project in China,No.20080738the Frontier Interdiscipline Program of Norman Bethune Health Science Center of Jilin University in China,No.2013106023
文摘Tissue engineering technologies offer new treatment strategies for the repair of peripheral nerve injury, hut cell loss between seeding and adhesion to the scaffold remains inevitable. A thermosensitive collagen hydrogel was used as an extracellular matrix in this study and combined with bone marrow mesenchymal stem cells to construct tissue-engineered peripheral nerve composites in vitro. Dynamic culture was performed at an oscillating frequency of 0.5 Hz and 35° swing angle above and below the horizontal plane. The results demonstrated that bone marrow mesenchymal stem cells formed membrane-like structures around the poly-L-lactic acid scaffolds and exhibited regular alignment on the composite surface. Collagen was used to fill in the pores, and seeded cells adhered onto the poly-L-lactic acid fibers. The DNA content of the bone marrow mesenchymal stem cells was higher in the composites constructed with a thermosensitive collagen hydrogel compared with that in collagen I scaffold controls. The cellular DNA content was also higher in the thermosensitive collagen hydrogel composites constructed with the thermosensitive collagen hydrogel in dynamic culture than that in static culture. These results indicate that tissue-engineered composites formed with thermosensitive collagen hydrogel in dynamic culture can maintain larger numbers of seeded cells by avoiding cell loss during the initial adhe-sion stage. Moreover, seeded cells were distributed throughout the material.
文摘背景:由于关节软骨的解剖、生理特点,其自我修复能力有限,故而如何修复大面积的软骨缺损(直径>4 mm)成为医学界备受瞩目的问题之一。伴随生物材料和组织工程学科的发展,通过支架技术尤其具有模拟细胞外基质微环境的水凝胶支架研究的深入发展,为软骨损伤修复提供了新的治疗思路。目的:就海藻酸盐的性质、海藻酸盐水凝胶支架的制备以及在软骨损伤修复中的研究进展进行综述。方法:利用计算机检索Web of Science、PubMed、万方和中国知网数据库,中文检索词为“海藻酸盐水凝胶、软骨组织工程或软骨、骨软骨、支架”,英文检索词为“alginate hydrogel,cartilage tissue engineering or chondro*,osteochondral,scaffold”,检索文献时间范围2016年1月至2020年12月。最终按入组标准筛选后纳入60篇文献进行综述分析。结果与结论:①海藻酸盐作为带负电荷的天然亲水性多糖,可通过修饰技术赋予传统海藻酸盐水凝胶更优良的机械性能、黏附性、生物降解性以及生物相容性等。②修饰后的海藻酸盐复合水凝胶支架利于维持种子细胞的正常形态、合成相应的细胞外基质,促进成软骨相关基因的表达,表现出优良的成软骨能力。③有报道证实海藻酸盐复合水凝胶支架可在动物体内形成与周边正常软骨相似的软骨组织,有效修复缺损部位。④因此,海藻酸盐复合水凝胶支架具备良好的促软骨修复能力,为软骨组织工程提供新的治疗思路,但该材料未来还需更完善的临床前试验数据的支撑,以推进其临床转化进程。
