The hydrogen and oxygen isotopic compositions of hydroxyl in clay mineral from a weathering profile： New proxies for paleo-climate change？

Recent studies suggest that the hydrogen and oxygen isotopic compositions of clay minerals can indicate paleoclimate.Here,we report mineralogy and stable isotopic records(d D and δ^(18)O_(OH))of a weathering profile located in the Fujian Province,aiming to validate whether hydroxyl stable isotopes can indicate paleo-precipitation and paleo-temperature.Our results indicate that the d D and δ^(18)O_(OH)changes in the kaolinite hydroxyl of the weathering profile are basically determined by the isotopic composition of paleo-meteoric water and paleotemperature,respectively.Nevertheless,whether the d D and δ^(18)O_(OH)of kaolinite can quantitatively indicate paleo-precipitation and paleo-temperature needs to be verified further,and especially,the structural oxygen isotopic composition that is the essential element for the kaolinite formation temperature calculation has to be constrained in future work.

Estimating distribution of water uptake with depth of winter wheat by hydrogen and oxygen stable isotopes under different irrigation depths

Crop root system plays an important role in the water cycle of the soil-plant-atmosphere continuum. In this study, com- bined isotope techniques, root length density and root cell activity analysis were used to investigate the root water uptake mechanisms of winter wheat （Triticum aesfivum L.） under different irrigation depths in the North China Plain. Both direct inference approach and multisource linear mixing model were applied to estimate the distribution of water uptake with depth in six growing stages. Results showed that winter wheat under land surface irrigation treatment （Ts） mainly absorbed water from 10-20 cm soil layers in the wintering and green stages （66.9 and 72.0%, respectively）; 0-20 cm （57.0%） in the jointing stage; 0-40 （15.3%） and 80-180 cm （58.1%） in the heading stage; 60-80 （13.2%） and 180-220 cm （35.5%） in the filling stage; and 0-40 （46.8%） and 80-100 cm （31.0%） in the ripening stage. Winter wheat under whole soil layers irrigation treatment （Tw） absorbed more water from deep soil layer than Ts in heading, filling and ripening stages. Moreover, root cell activity and root length density of winter wheat under TW were significantly greater than that of Ts in the three stages. We concluded that distribution of water uptake with depth was affected by the availability of water sources, the root length density and root cell activity. Implementation of the whole soil layers irrigation method can affect root system distribution and thereby increase water use from deeper soil and enhance water use efficiency.

Characteristics of Hydrogen and Oxygen Isotopes and Noble Gas Isotopes in the Groundwater of Weishan, Wudalianchi, Northeast China

According to the hydrochemical characteristics, hydrogen and oxygen isotope characteristics and the ratio of noble gas isotopes of the sandstone aquifer and basalt aquifer, this study calculated the recharge temperature and residence time of groundwater in the Weishan area of Wudalianchi, also calculating the contribution of noble gas components from different sources to the samples. Based on the characteristics of hydrogen and oxygen isotopes and noble gases Xe and Ne, the recharge altitude and recharge temperature of the two aquifers were estimated, and the recharge temperature fitting with the NGT model as verified, the results showing that the main recharge altitude of groundwater in the region was 500–600 m, the recharge temperature being 2–7°C. He_(eq) and He_(ea) of the samples have been simulated using the OD model, the content of radioactive ~4He in the crust being obtained, the groundwater ages under the two conditions(closed condition and open condition) both being simulated. The results show that groundwater from the sandstone layer water is older than groundwater from the basalt layer. Hydrochemical characteristics and noble gas isotope ratios indicate that in the basalt aquifer and sandstone aquifer in the Weishan area, in addition to atmospheric and crustal helium, there is also an input of mantle-derived helium. The fault constitutes the uplift channel for groundwater containings mantle components, which results in the mantle source composition in water samples near the fault being much higher than those form non-fault areas.

Ore genesis of Badi copper deposit, northwest Yunnan Province, China： evidence from geology, fluid inclusions, and sulfur, hydrogen and oxygen isotopes

The Badi copper deposit is located in Shangjiang town, Shangri-La County, Yunnan Province. Tectonically, it belongs to the Sanjiang Block. Vapor-liquid two-phase fluid inclusions, CO2-bearing fluid inclusions, and daugh- ter-beating inclusions were identified in sulfide-rich quartz veins. Microthermometric and Raman spectroscopy studies revealed their types of ore-forming fluids： （1） low-tem- perature, low-salinity fluid; （2） medium-temperature, low salinity CO2-bearing; and （3） high-temperature, Fe-rich, high sulfur fugacity. The δ^18O values of chalcopyrite- bearing quartz ranged from 4.96‰ to 5.86%0, with an average of 5.40%0. The δD values of ore-forming fluid in equilibrium with the sulfide-bearing quartz were from - 87‰ to - 107‰, with an average of - 97.86%0. These isotopic features indicate that the ore-forming fluid is a mixing fluid between magmatic fluid and meteoric water. The δ^34S values of chalcopyrite ranged from 13.3‰ to 15.5‰, with an average of 14.3‰. Sulfur isotope values suggest that the sulfur in the deposit most likely derived from seawater. Various fluid inclusions coexisted in the samples; similar homogenization temperature to different phases suggests that the Badi fluid inclusions might have been captured under a boiling system. Fluid boiling caused by fault activity could be the main reason for the mineral precipitation in the Badi deposit.

Hydrogen and Oxygen Isotopes of Fluid Inclusion in Halite,Northern Shaanxi Salt Basin China

The Ordovician was an important transitional period for global climate and organic evolution,the global was in the flood and glacial,Onganism was extinction(Zhan,2007;Trotter et al.,2008;Axel et al.,2010).Under the influence

Experimental study of relationship between average isotopic fractionation factor and evaporation rate

Isotopic fractionation is the basis of tracing the water cycle using hydrogen and oxygen isotopes. Isotopic fractionation factors in water evaporating from free water bodies are mainly affected by temperature and relative humidity, and vary significantly with these atmospheric factors over the course of a day. The evaporation rate （E） can reveal the effects of atmospheric factors. Therefore, there should be a certain functional relationship between isotopic fractionation factors and E. An average isotopic fractionation factor （ t~＊ ） was defined to describe isotopic differences between vapor and liquid phases in evaporation with time intervals of days. The relationship between or＊ and E based on the isotopic mass balance was investigated through an evaporation pan experiment with no inflow. The experimental results showed that the isotopic compositions of residual water were more enriched with time; tr＊ was affected by air temperature, relative humidity, and other atmospheric factors, and had a strong functional relation with E. The values of 0~＊ can be easily calculated with the known values of E, the initial volume of water in the pan, and isotopic compositions of residual water.

Experimental study on stable isotopic fractionation of evaporating water under varying temperature

The variation of stable isotope ratios in natural waters provides valuable information that can be used to trace water movement. Evaporation plays a crucial role in determining the variation of stable isotopes. In this paper, several evaporation experiments were conducted in order to study the stable isotopic fractionation mechanism of water and analyze the influence of different temperatures on evaporation fractionation. Three group experiments of water evaporation under different temperatures and initial isotopic values were carried out. The results show that fractionation factors of hydrogen and oxygen may increase with temperature, and the average enrichment degree of hydrogen isotope D is 3.432 times that of oxygen isotope 18O. The results also show that the isotopic composition of the initial water has little influence on water evaporation fractionation, which is mainly affected by the state variables in the evaporation process, such as temperature. This research provides experimental data for further understanding the evaporation fractionation mechanism.

Stable isotopes of water as a tracer for revealing spatial and temporal characteristics of groundwater recharge surrounding Qinghai Lake,China

Studying spatial and temporal characteristics of regional groundwater recharge will guide the scientific management and sustainable development of regional water resources.This study investigated stable isotopes(δ^(18)O and δ^(2) H)of precipitation,groundwater,river water and lake water during 2019-2020 in Qinghai Lake Basin to reveal the spatial and temporal characteristics of groundwater recharge.The local meteoric water line was simulated using ordinary least squares regression(δ^(2) H=7.80δ^(18)O+10.60).The local evaporation lines of the river water,lake water and groundwater were simulated asδ^(2) H=6.21δ^(18)O-0.72,δ^(2) H=5.73δ0-3.60 and δ^(2) H=6.59δ0+1.76,respectively.The δ^(2) H and δ^(18)O of river water and groundwater were in more depleted values due to the recharge by precipitation at high altitudes or precipitation effects,and theδ^(2) H andδ^(18)O of the lake water were in more enriched values because of evaporation.The relationship between the δ^(2) H and δ^(18)O of groundwater and river water was not significantly different,indicating a strong hydrological connection between the groundwater and river water surrounding Qinghai Lake.Additionally,the maximum values of δ^(18)O and the minimum values of lc-excess of groundwater in most regions were both in August,and the minimum values of δ^(18)O and the maximum values of lc-excess of groundwater in most regions were both in October.Therefore,the groundwater was recharged by soil water with strong evaporation in August and recharged by precipitation at high altitudes in October.The recharge rate of groundwater was relatively fast in areas with large slopes and large hydraulic gradients(e.g.,south of Qinghai Lake),and in areas with strong hydrological connections between the groundwater and river water(e.g.,the Buha River Valley).Those results can provide data support for protection and utilization of water resources in Qinghai Lake Basin,and provide reference for groundwater research in closed lake basins on the Qinghai-Tibet Plateau.

Water sources of plants and groundwater in typical ecosystems in the lower reaches of the Heihe River Basin

Stable oxygen and hydrogen isotopic compositions （δ18O and δD） of soil water and shallow groundwater of a riparian forest, an artificial shrub forest, and Gobi of the lower reaches of the Heihe River Basin are used to study the recharge water sources of those ecosystems. IsoSource software is used to determine the δ180 values for root water of Populous euphratica and Tamarix ramosissima in the riparian forest ecosystem, Haloxylon ammodendron in the artificial shrub forest, and Reaumuria soongorica in the Gobi, as well as for local soil water and groundwater, and precipitation in the upper reaches of the Heihe River Basin. Our results showed that soil water and shallow groundwater of the riparian forest and the artificial shrub forest were recharged by river water which originated from precipitation in the upper reaches, and strong evaporation occurred in the artificial shrub forest. Soil water of the Gobi was not affected by Heihe River water due to this area being far away from the river channel. The main water sources of Populous euphratica were from 40-60-cm soil water and groundwater, and of Tamarix ramosissima were from 40-80-cm soil water in the riparian forest ecosystem. In the artificial forest, Haloxylon ammodendron used 200-cm saturated-layer soil water and shallow groundwater. The Reaumuria soongorica mainly used soil water from the 175-200-cm depth in the Gobi. Therefore, soil water and groundwater are the main water sources which maintain survival and growth of the plants in the extremely arid regions of the lower reaches of the Heihe River Basin.

Study on Change Rules and Influencing Factors of Soil Moisture in Huaibei Plain

[Objectives]To study the relationship between soil water,groundwater burial depth,and precipitation for summer maize in Huaibei Plain.[Methods]The atmospheric precipitation,soil water and groundwater for the growth period of summer maize in Huaibei Plain were analyzed using the 26-year long series of data from the Wudaogou Hydrological Experimental Station,combined with the hydrogen and oxygen stable isotope tracing method.[Results]The average soil moisture content of summer maize in different growth periods showed a trend of first decreasing,then increasing and then decreasing with the increase of soil depth.The average soil moisture content was the lowest at the surface soil layer.From the characteristic values of hydrogen and oxygen isotopes of atmospheric precipitation,soil water and groundwater,it can be known that the average values ofδ18O andδD of soil water decreased with the increase of soil depth,indicating that soil moisture evaporation leads to the enrichment of soil heavy isotopes,and the degree of enrichment decreased from the surface layer to the deep layer of the soil.The seasonal variation of the stable isotope of hydrogen and oxygen in soil water declined with the increase of soil depth.The soil water changes at 30 cm and 50 cm soil depths were the most obvious.The soil was easily recharged by precipitation,and soil evaporation was relatively strong.[Conclusions]The research results are favorable for in-depth understanding of the regional water cycle process,and are expected to provide a certain scientific basis for realizing the efficient and sustainable use of regional groundwater.

Application of Stable Isotope Tracing Technologies in Identification of Transformation among Waters in Sanjiang Plain,Northeast China

In order to investigate the transformation among the precipitation,groundwater,and surface water in the Sanjiang Plain,Northeast China,precipitation and groundwater samples which were collected at the meteorological station of the Sanjiang Mire Wetland Experimental Station,Chinese Academy of Sciences and the surface water which collected from the Wolulan River were used to identify the transformation of three types of water.The isotope composition of different kinds of water sources were analyzed via stable isotope(deuterium and oxygen-18) investigation of natural water.The results show a clear seasonal difference in the stable isotopes in precipitation.During the cold half-year,the mean stable isotope in precipitation in the Sanjiang Plain reaches its minimum with the minimum temperature.The δ18O and δD values are high in the rainy season.In the Wolulan River,the evaporation is the highest in August and September.The volume of evaporation and the replenishment to the river is mostly same.The groundwater is recharged more by the direct infiltration of precipitation than by the river flow.The results of this study indicate that the water bodies in the Sanjiang Plain have close hydrologic relationships,and that the transformation among each water system frequently occurs.

Hydrochemistry of the Gasikule Salt Lake,Western Qaidam Basin of China

The Qaidam Basin is a large intermontane depression in Qinghai Province,China,which located on the northern margin of the Tibet plateau,and surrounded by the Qilian,Kunlun and Aljun mountains which rise to more than 5000m.Some 27 salt lakes occur within the basin,occupying an area of approximately 1500 km2.Additionally,there are extensive areas of dry playas.Together,the playas and salt lakes cover about one quarter of the total basin area.Whereas the western

Evaporation Stage of Paleo-saline Lake in the Sichuan Basin, China: Insight from Fluid inclusions in Halite

Sichuan Basin is one of the most important marine–salt forming basins in China. The Simian and Triassic have a large number of evaporites. The Triassic strata have found a large amount of polyhalite and potassium-rich brine. However, no soluble potassium salt deposit were found. In this study, the halite in well Changping 3 which is located at the eastern part of the Sichuan basin was studied using the characteristics, hydrogen and oxygen isotopes of the fluid inclusion in halite to reconstruct the paleoenvironment. The salt rocks in well Changping 3 can be divided into two types: grey salt rock and orange salt rock. The result shows that the isotopic composition of the halite fluid inclusion is distinct from the global precipitation line reflecting that the salt formation process is under strong evaporation conditions and the climate is extremely dry. At the same time, compared with the hydrogen and oxygen isotopes of brine in the Sichuan Basin and the hydrous isotope composition of the inclusions in the salt inclusions of other areas in China, it is shown that the evaporation depth of the ancient seawater in the Sichuan Basin was high and reached the precipitation of potassium and magnesium stage.

Stable isotope techniques in plant water sources:a review

The stable hydrogen and oxygen isotopes widely exist in various kinds of natural water.Plants have to cope with various water sources:rainwater,soil water,groundwater,sea water,and mixtures.These are usually characterized by different isotopic signatures (18O/16O and D/H ratios).Because there are relative abundance variations in water,and plant roots do not discriminate against specific water isotopes during water uptake,hydrogen and oxygen stable isotope ratios of water within plants provide new information on water sources,interactions between plant species and water use patterns under natural conditions.At present,the measurement of δD,δ18O composition of various potential water sources and stem water has become significant means to identify plant water sources.Based on previous studies,this review highlights recent advances such as theory basis,methodology,as well as different spatial and temporal scales,and existed questions and prospects.Stable isotope techniques for estimating plant water sources have provided valuable tools for conducting basic and applied research.Future studies emphasize the modification of preparing methods,isotope technique combined with other measurements,and aerial organs of plant water source should be en-couraged.

A Study of Ore-forming Fluids in the Shimensi Tungsten Deposit,Dahutang Tungsten Polymetallic Ore Field,Jiangxi Province,China

The Dahutang tungsten polymetallic ore field is located north of the Nanling W-Sn polymetallic metallogenic belt and south of the Middle—Lower Yangtze River Valley Cu-Mo-Au-Fe porphyry-skarn belt.It is a newly discovered ore field,and probably represents the largest tungsten mineralization district in the world.The Shimensi deposit is one of the mineral deposits in the Dahutang ore field,and is associated with Yanshanian granites intruding into a Neoproterozoic granodiorite batholith.On the basis of geologic studies,this paper presents new petrographic,microthermometric,laser Raman spectroscopic and hydrogen and oxygen isotopic studies of fluid inclusions from the Shimensi deposit.The results show that there are three types of fluid inclusions in quartz from various mineralization stages：liquid-rich two-phase fluid inclusions,vapor-rich two-phase fluid inclusions,and three-phase fluid inclusions containing a solid crystal,with the vast majority being liquid-rich two-phase fluid inclusions.In addition,melt and melt-fluid inclusions were also found in quartz from pegmatoid bodies in the margin of the Yanshanian intrusion.The homogenization temperatures of liquid-rich two-phase fluid inclusions in quartz range from 162 to 363℃ and salinities are 0.5wt%-9.5wt%NaCI equivalent.From the early to late mineralization stages,with the decreasing of the homogenization temperature,the salinity also shows a decreasing trend.The ore-forming fluids can be approximated by a NaCl-H_2O fluid system,with small amounts of volatile components including CO_2,CH_4 and N_2,as suggested by Laser Raman spectroscopic analyses.The hydrogen and oxygen isotope data show that δ5D_（V-smow） values of bulk fluid inclusions in quartz from various mineralization stages vary from-63.8‰ to-108.4‰,and the δ^（18）O_（H2O） values calculated from the δ^（18）O_（V-）smow values of quartz vary from-2.28‰ to 7.21‰.These H-O isotopic data are interpreted to indicate that the ore-forming fluids are mainly composed of magmatic water in the early stage,and meteoric water was added and participated in mineralization in the late stage.Integrating the geological characteristics and analytical data,we propose that the ore-forming fluids of the Shimensi deposit were mainly derived from Yanshanian granitic magma,the evolution of which resulted in highly differentiated melt,as recorded by melt and melt-fluid inclusions in pegmatoid quartz,and high concentrations of metals in the fluids.Cooling of the ore-forming fluids and mixing with meteoric water may be the key factors that led to mineralization in the Dahutang tungsten polymetallic ore field.

Source of Ore-Forming Fluid and Material in the Baiyun Gold Deposit, Liaoning Province, NE China: Constraints from H-O-S-Pb Isotopes and in-situ Analyses of Au-Bearing Pyrites

The Baiyun deposit is a large gold deposit at the western end of the Liaoji rift zone in Liaoning Province, which has produced both auriferous quartz-vein type and altered-rock type mineralization. The ore bodies are mainly hosted in schist from the Gaixian Formation of the Liaohe Group. A detailed field geological survey showed that the quartz-vein type gold ore bodies are distributed in the near EW-trending and occur in the extensional tectonic space of schist in the Gaixian Formation, and the altered-rock type gold ore bodies are distributed in the near EW-trending structural belt and occur near in the Gaixian Formation of biotite schist, biotite granulite, marble and the upper footwall of dike. To further elucidate the source of ore-forming fluid and material in the Baiyun gold deposit, the H-O isotopes for quartz, S and Pb isotopes, in-situ trace elements for sulfides from quartz-vein and altered-rock type mineralization were studied. The H-O isotopic δD_(V-SMOW) and δ^(18)O_(H2O) values of the auriferous quartz range were from-88.8‰ to-82.2‰ and-1.95‰ to 4.85‰, respectively, suggests that the ore-forming fluids were mainly magmatic water with minor meteoric water. The distribution ranges of in-situ S isotopic compositions of Au-bearing pyrite in the quartz-vein type and altered-rock type ores were-8.38‰–-10.47‰(with average values of-7.89‰) and 11.38‰– 17.52‰(with average values of 11.55‰), respectively, indicating that the S isotopic compositions of the two ore types were clearly different. The in-situ Pb isotopic ratios changed almost uniformly, which showed that they had the same lead isotopic source. Based on the analysis of S and Pb isotopic compositions, the metallogenic materials in the Baiyun gold deposit were primarily from deep magma, and some wall rock materials may have been mixed in the metallogenic process. Co/Ni diagram shows that most Au-bearing pyrites have magmatic-hydrothermal or sedimentary alteration properties, and Au/As ratios were between 0.001 and 0.828(the average value was 0.07), indicating that the ore-forming fluid in the Baiyun gold deposit may have been deep magma. Combining the geological, trace element, and isotopic data, as well as data from previous studies, we propose that the Baiyun gold deposit is a magmatic-hydrothermal ore deposit.

Using water isotopes and hydrogeochemical evidences to characterize groundwater age and recharge rate in the Zhangjiakou area,North China

Despite the increasing depletion of the groundwater at the Zhangjiakou aquifer system in the northwest of Beijing-Tianjin-Hebei region,little information is available on the hydrological process of groundwater in this region.In this study,we utilized water isotopes composition(51sO,5D and 3H)of groundwater,river and precipitation to identify the characteristics of hydrochemistry,groundwater age and recharge rates in different watersheds of the Zhangjiakou area.Results showed that the river water and groundwater could be characterized as HCO3-Mg Na,HCO3 CI-Na and HCO3-Mg Na,HCO3 CI-Na,HCO3 CI-Na Mg types,respectively.The 5D and 5180 values in precipitation were linearly correlated,which is similar to the Global Meteorological Water Line(GMWL).Furthermore,the decreasing values of the 6D and 5180 from precipitation to surface water and groundwater indicate that groundwater is mainly recharged by atmospheric precipitation.In addition,the variation of 3H concentration with depth suggests that groundwater shallower than around 100 m is generally modern water.In contrast,groundwater deeper around 100 m is a mixture of modern and old waters,which has longer residence times.Groundwater showed a relatively low tritium concentration in the confined aquifers,indicating the groundwater recharged might be relatively old groundwater of over 60 years.The flow velocity of the groundwater in the study area varied from 1.10 to 2.26 m/a,and the recharge rates ranged from 0.034 to 0.203 m/a.The obtained findings provide important insights into understanding the groundwater recharge sources and hydrochemistry in the Zhangjiakou area,in turn developing a sustainable groundwater management plan.

Movement of lateral hyporheic flow between stream and groundwater

The hyporbeic zone plays an important role in groundwater and stream water quality protection. To investigate the stream-groundwater interaction mechanisms in the lateral hyporheic zone, this study examined Ma＇an Creek in Chongqing during the dry season from December 2015 to April 2016. The water level, water temperature, pH and CF concentration in the hyporheic zone and groundwater were monitored in situ. The sediment permeability coefficient, stable isotopes of hydrogen and oxygen and concentration of DOC were analyzed. The results show that the water level changes of hyporheic zone and the movement of hyporheic flow were influenced significantly by the permeability coefficient of sediment. The hyporheic flow approximately 10 cm from the stream bank was clearly affected by precipitation infiltration and evapotranspiration. During the study period, the groundwater recharged the stream, and the impact of groundwater on the hyporheic flow gradually decreased with the flow path. The hyporheic flow approximately 30 cm from the stream bank was still mainly affected by groundwater. Approximately 10-30 cm from the stream bank, the mixing of groundwater with precipitation and stream water intensified. Due to the sediment properties, moisture accumulated approximately 10 cm from the stream bank and drained into the stream via hyporheic flow, with potential impacts on stream water quality.

Glacier meltwater runoff process analysis using δD and δ^(18)O isotope and chemistry at the remote Laohugou glacier basin in western Qilian Mountains, China

Stable hydrogen and oxygen isotope has important implication on water and mois- ture transportation tracing research. Based on stable hydrogen （6D） and oxygen （6180） isotope using a Picarro Ll102-i and water chemistry （e.g. major ions, pH, EC and TDS） meas- urement, this study discussed the temporal variation and characteristics of stable hydrogen and oxygen isotope, chemistry （e.g. TDS, pH, EC, Ca^2^, Mg2＋, Na^＋ and CI） in various water bodies including glacier meltwater runoff, ice and snow, and precipitation at the Laohugou g^acier basin during June 2012 to September 2013. Results showed that 6D and δ18O in the meltwater runoff varied obviously with the temporal change from June to September, showing firstly increasing trend and then decreasing trend, with the highest values in July with high air temperature and strong glacier melting, which could indicate the temporal change of glacier melting process and extent. Variations of 6D and δ18O in the runoff were similar with that of snow and ice on the glacier, and the values were also above the GMWL, which probably im- plied that the glacier runoff was mainly originated from glacier melting and precipitation supply The glacier meltwater chemical type at the Laohugou glacier basin were mainly composed by Ca-Na-HCO3-SO4 and Ca-Mg-HCO3-SO4, which also varied evidently with the glacier melting process in summer. By analyzing the temporal change of stable hydrogen and oxygen isotope and chemistry in the melting period, we find it is easy to separate the components of the snow and ice, atmospheric precipitation and melt-runoff in the river, which could reflect the change process of glacier melting during the melting period, and thus this work can contribute to the glacier runoff change study of large-scale region by stable isotope and geochemical method in future.

Water sources for typical desert vegetation in the Ebinur Lake basin

In arid and semi-arid environments,desert vegetation plays an important role in preventing soil erosion by wind and helps maintain the stability of desert and oasis ecosystems.Four types of typical desert vegetation,namely Populus euphratica,Haloxylon ammodendron,Nitraria sibirica,and Halostachs caspica,corresponding to different habitats(i.e.,river bank,sand dune,desert,and salt marsh)were chosen as the model vegetation in this research.Theδ^(2)H andδ^(18)O for rainwater,soil water,and plant water were applied to identify the water sources and quantify the proportions of different water sources used over the entire plant growth period(from March to October).The results showed that the precipitationδ^(2)H andδ^(18)O in the Ebinur Lake basin varied from-142.5‰to-0.6‰and from-20.16‰to 1.20‰,respectively.The largestδ^(2)H andδ^(18)O values occurred in summer and the smallest in winter.The soil waterδ^(2)H andδ^(18)O of the four habitats decreased gradually with increasing depth.Theδ^(2)H andδ^(18)O values of water extracted from the stems of the four plants had similar variation trends,that is,the maximum was observed in spring and the minimum in summer.Among the four plants,H.caspica had the highest stable isotopic values in the stem water,followed by N.sibirica,H.ammodendron,and P.euphratica.The water sources and utilization ratios of desert vegetation varied across different growth stages.Throughout the growing period,H.ammodendron mainly used groundwater,whereas the water source proportions used by N.sibirica varied greatly throughout the growing season.In spring,plants mainly relied on surface soil water,with a contribution rate of 80%-94%.However,in summer,the proportion of deep soil water used was 31%-36%;and in autumn,the proportion of middle soil water used was 33%-36%.H.caspica mainly relied on topsoil water in spring and autumn,and the proportion of soil water in the middle layer slightly increased to 20%-36%in summer.P.euphratica mainly used intermediate soil water in spring with a utilization rate of 53%-54%.In summer,groundwater was the main source,with a utilization rate of 72%-88%,and only 2%-5%came from river water,whereas in autumn,the river water utilization rate rose to 11%-21%.The results indicated that there were significant differences in water use sources during the growing period for desert vegetation in arid areas.This research provides a theoretical basis for understanding water use mechanisms,water adaptation strategies,and vegetation restoration and management in arid areas.