Nitric oxide（NO）and hydrogen peroxide（H2O2）are essential signaling molecules with key roles in auxin induced adventitious root formation in many plants.However,whether they are the sole determinants for adventitio...Nitric oxide（NO）and hydrogen peroxide（H2O2）are essential signaling molecules with key roles in auxin induced adventitious root formation in many plants.However,whether they are the sole determinants for adventitious root formation is worth further study.In this study,endogenous NO and H2O2 were monitored in tea cutting with or without indole-3-butyric acid（IBA）treatment by using the fluorescent probes diaminofluorescein diacetate（DAF-2DA）and 2＇,7＇-dichlorodihydrofluorescein diacetate（DCF-DA）,respectively.The overproduction of NO and H2O2 was detected in the rooting parts of tea cuttings treated with or without IBA.But little NO and H2O2 was detected before the initiation phase of tea cuttings even with IBA treatment indicating that they might be not directly induced by IBA.Further carbon and nitrogen analysis found that the overproduction of NO and H2O2 were coincident with the consumption of soluble sugars and the assimilation of nitrogen.These results suggest that rooting phases should be taken into consideration with the hypothesis that auxin induces adventitious root formation via NO-and H2O2-dependent pathways and sink establishment might be a prerequisite for NO and H2O2 mediated adventitious root formation.展开更多
[Objective] The study aimed to extract fulvic acid from brown coal using N-Mn-TiO2 as a catalyst and H2O2 or HNO3 as an oxidizer. [Method] The effects of catalyst N-Mn-TiO2 on the yield and structure of fuMc acid were...[Objective] The study aimed to extract fulvic acid from brown coal using N-Mn-TiO2 as a catalyst and H2O2 or HNO3 as an oxidizer. [Method] The effects of catalyst N-Mn-TiO2 on the yield and structure of fuMc acid were studied, and the content of functional groups in fulvic acid was analyzed qualitatively and quantitatively. [ Reselt] Two catalysts could improved the yield of fulvic acid, that is, catalyst 1 （N： Mn： 13 = 16： 0.001：1, roasting temperature was 400 ℃） and catalyst 2 （N： Mn： Ti = 16： 0.001：1, roasting temperature was 100 ℃） increased OFA yield by 10.69% and 32.17% and NFA by 8.61% and 7.49% respectively. After the addition of catalysts, the content of total acid radicals in OFA changed little, and carboxyi content increased slightly, but phenolic hydroxyl content decreased. When HNO3 was used as an oxidizer, the content of total acid radicals and phenolic hydroxyl in NFA decreased. In addition, the structure of OFA was different from that of NFA. [Condusion] The research could provide scientific references for the development and application of brown coal in future.展开更多
基金indebted to the earmarked fund for China Agriculture Research System (CARS-19)the Innovation Project for Agricultural Sciences and Technology from the Chinese Academy of Agricultural Sciences (CAAS-ASTIP2017-TRICAAS) for their financial supports
文摘Nitric oxide（NO）and hydrogen peroxide（H2O2）are essential signaling molecules with key roles in auxin induced adventitious root formation in many plants.However,whether they are the sole determinants for adventitious root formation is worth further study.In this study,endogenous NO and H2O2 were monitored in tea cutting with or without indole-3-butyric acid（IBA）treatment by using the fluorescent probes diaminofluorescein diacetate（DAF-2DA）and 2＇,7＇-dichlorodihydrofluorescein diacetate（DCF-DA）,respectively.The overproduction of NO and H2O2 was detected in the rooting parts of tea cuttings treated with or without IBA.But little NO and H2O2 was detected before the initiation phase of tea cuttings even with IBA treatment indicating that they might be not directly induced by IBA.Further carbon and nitrogen analysis found that the overproduction of NO and H2O2 were coincident with the consumption of soluble sugars and the assimilation of nitrogen.These results suggest that rooting phases should be taken into consideration with the hypothesis that auxin induces adventitious root formation via NO-and H2O2-dependent pathways and sink establishment might be a prerequisite for NO and H2O2 mediated adventitious root formation.
基金Supported by the Bidding Project of Qujing Normal University(2011ZB005)
文摘[Objective] The study aimed to extract fulvic acid from brown coal using N-Mn-TiO2 as a catalyst and H2O2 or HNO3 as an oxidizer. [Method] The effects of catalyst N-Mn-TiO2 on the yield and structure of fuMc acid were studied, and the content of functional groups in fulvic acid was analyzed qualitatively and quantitatively. [ Reselt] Two catalysts could improved the yield of fulvic acid, that is, catalyst 1 （N： Mn： 13 = 16： 0.001：1, roasting temperature was 400 ℃） and catalyst 2 （N： Mn： Ti = 16： 0.001：1, roasting temperature was 100 ℃） increased OFA yield by 10.69% and 32.17% and NFA by 8.61% and 7.49% respectively. After the addition of catalysts, the content of total acid radicals in OFA changed little, and carboxyi content increased slightly, but phenolic hydroxyl content decreased. When HNO3 was used as an oxidizer, the content of total acid radicals and phenolic hydroxyl in NFA decreased. In addition, the structure of OFA was different from that of NFA. [Condusion] The research could provide scientific references for the development and application of brown coal in future.
