Summary We have identified hpm91, a Chlamydomonas mutant lacking Proton Gradient Regulation5 （PGRS） capable of producing hydrogen （H2） for 25 days with more than 3o-fold yield increase compared to wild type. Thus,...Summary We have identified hpm91, a Chlamydomonas mutant lacking Proton Gradient Regulation5 （PGRS） capable of producing hydrogen （H2） for 25 days with more than 3o-fold yield increase compared to wild type. Thus, hpm91 displays a higher capacity of H2 production than a previously characterized pgr5 mutant. Physiological and biochemical characterization of hpm91 reveal that the prolonged H2 production is due to enhanced stability of PSII, which correlates with increased reactive oxygen species （ROS） scavenging capacity during sulfur depriva- tion. This anti-ROS response appears to protect the photosynthetic electron transport chain from photo- oxidative damage and thereby ensures electron supply to the hydrogenase.展开更多
基金supported by grants from the National Natural Science Foundation of China (No. 31470340 and No. 31270288)National Basic Research Program of China (973Program, No. 2015CB150100)+1 种基金CAS XDB (No. 17030300)the Foreign Expert Program (J-D.R.)
文摘Summary We have identified hpm91, a Chlamydomonas mutant lacking Proton Gradient Regulation5 （PGRS） capable of producing hydrogen （H2） for 25 days with more than 3o-fold yield increase compared to wild type. Thus, hpm91 displays a higher capacity of H2 production than a previously characterized pgr5 mutant. Physiological and biochemical characterization of hpm91 reveal that the prolonged H2 production is due to enhanced stability of PSII, which correlates with increased reactive oxygen species （ROS） scavenging capacity during sulfur depriva- tion. This anti-ROS response appears to protect the photosynthetic electron transport chain from photo- oxidative damage and thereby ensures electron supply to the hydrogenase.
