The surfactant-coated Candida rugosa lipase was used as catalyst for hydrolysis of olive oil in two-phase system consisting of olive oil and phosphate buffer without organic solvent. For both the coated and native lip...The surfactant-coated Candida rugosa lipase was used as catalyst for hydrolysis of olive oil in two-phase system consisting of olive oil and phosphate buffer without organic solvent. For both the coated and native lipases,the optimal buffer/oil volume ratio of 1.0, aqueous pH 6.8 and reaction temperature 30℃ were determined. The maximum activity of the coated lipase was ca 1.3 times than that of the native lipase. The half-life of the coated lipase in olive oil and the native lipase in phosphate buffer was ca 9 h and 12 h, and the final residual activity was 27% and 20% of their initial values, respectively. The final substrate conversion by the coated lipase was ca 20% higher than that of the native lipase.展开更多
本文通过单因素实验,分别考察超声时间、超声温度、p H、酶添加量对脂肪酸水解率的影响,结合响应面实验优化超声波辅助脂肪酶水解红花籽油的工艺条件,并比较超声波辅助酶解和水浴酶解中米氏常数及表观动力学的变化。结果表明:最佳酶解...本文通过单因素实验,分别考察超声时间、超声温度、p H、酶添加量对脂肪酸水解率的影响,结合响应面实验优化超声波辅助脂肪酶水解红花籽油的工艺条件,并比较超声波辅助酶解和水浴酶解中米氏常数及表观动力学的变化。结果表明:最佳酶解条件为:超声时间10 h,超声温度28℃,p H7.0,米曲霉脂肪酶添加量为280 U/g,水油比1.6∶1(v/v),此时红花籽油的水解率达到94.47%。对其进行表观动力学研究,测得超声波辅助脂肪酶水解红花籽油的米氏常数Km=4.63 m L/g,表观活化能Ea=2.05 k J/mol,比水浴酶解条件下米氏常数Km=14.93 m L/g和表观活化能Ea=2.40 k J/mol均降低,证明超声波辅助脂肪酶能促进红花籽油的水解。展开更多
基金National Natural Science Foundation of China(No.29876031)
文摘The surfactant-coated Candida rugosa lipase was used as catalyst for hydrolysis of olive oil in two-phase system consisting of olive oil and phosphate buffer without organic solvent. For both the coated and native lipases,the optimal buffer/oil volume ratio of 1.0, aqueous pH 6.8 and reaction temperature 30℃ were determined. The maximum activity of the coated lipase was ca 1.3 times than that of the native lipase. The half-life of the coated lipase in olive oil and the native lipase in phosphate buffer was ca 9 h and 12 h, and the final residual activity was 27% and 20% of their initial values, respectively. The final substrate conversion by the coated lipase was ca 20% higher than that of the native lipase.
文摘本文通过单因素实验,分别考察超声时间、超声温度、p H、酶添加量对脂肪酸水解率的影响,结合响应面实验优化超声波辅助脂肪酶水解红花籽油的工艺条件,并比较超声波辅助酶解和水浴酶解中米氏常数及表观动力学的变化。结果表明:最佳酶解条件为:超声时间10 h,超声温度28℃,p H7.0,米曲霉脂肪酶添加量为280 U/g,水油比1.6∶1(v/v),此时红花籽油的水解率达到94.47%。对其进行表观动力学研究,测得超声波辅助脂肪酶水解红花籽油的米氏常数Km=4.63 m L/g,表观活化能Ea=2.05 k J/mol,比水浴酶解条件下米氏常数Km=14.93 m L/g和表观活化能Ea=2.40 k J/mol均降低,证明超声波辅助脂肪酶能促进红花籽油的水解。
