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基于聚倍半硅氧烷微球的亲水/反相混合模式色谱填料的制备与评价 被引量:6
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作者 吕倩楠 《色谱》 CAS CSCD 北大核心 2017年第9期927-933,共7页
亲水/反相混合模式色谱应用广泛,但pH使用范围有限,不利于碱性药物的分离。该工作利用巯基-烯基点击化学合成了单分散多孔的半胱氨酸改性乙烯基功能化聚甲基倍半硅氧烷(C-V-PMSQ)微球。元素分析表明半胱氨酸成功键合在微球表面。C-V-PMS... 亲水/反相混合模式色谱应用广泛,但pH使用范围有限,不利于碱性药物的分离。该工作利用巯基-烯基点击化学合成了单分散多孔的半胱氨酸改性乙烯基功能化聚甲基倍半硅氧烷(C-V-PMSQ)微球。元素分析表明半胱氨酸成功键合在微球表面。C-V-PMSQ微球为介孔结构,单分散性好且具有优良的化学稳定性。以几种常见的核苷和核酸碱基作为测试样品,考察其色谱保留行为,溶质的保留因子随流动相中水相含量的变化呈现典型的U型曲线,表明C-V-PMSQ固定相具有亲水/反相的双重保留特征。使用该固定相可以分离苯的同系物及一系列亲水性与疏水性化合物。另外在高碱性流动相条件下利用亲水和反相模式成功分离了中药苦参中的3种主要活性成分,表明它在分离碱性药物方面具有较大的优势。 展开更多
关键词 亲水/反相 混合模式 色谱填料 聚倍半硅氧烷 碱性化合物
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Challenges in Analysis of Hydrophilic Metabolites Using Chromatography Coupled with Mass Spectrometry 被引量:1
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作者 Qingyu Hu Huiru Tang Yulan Wang 《Journal of Analysis and Testing》 EI 2020年第3期140-162,共23页
Hydrophilic metabolites play important roles in cellular energy metabolism,signal transduction,immunity.However,there are challenges in both identification and quantification of the hydrophilic metabolites due to thei... Hydrophilic metabolites play important roles in cellular energy metabolism,signal transduction,immunity.However,there are challenges in both identification and quantification of the hydrophilic metabolites due to their weak interactions with C18-reversed-phase liquid chromatography(RPLC),leading to poor retention of hydrophilic metabolites on the columns.Many strategies have been put forward to increase the retention behavior of hydrophilic metabolites in the RPLC system.Non-derivatization methods are mainly focused on the development of new chromatographic techniques with different separation mechanisms,such as capillary electrophoresis,ion-pairing RPLC etc.Derivatization methods improve the hydrophobicity of metabolites and can enhance the MS response.This review mainly focused on the illustration of challenges of LCMS in the analysis of hydrophilic metabolomics field,and summarized the non-derivatization and derivatization strategies,with the intention of providing multiple choices for analysis of hydrophilic metabolites. 展开更多
关键词 hydrophilic metabolites hydrophilic interaction chromatography Ion-pairing reversed-phase liquid chromatography Ion chromatography Capillary electrophoresis DERIVATIZATION
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