Lunar In Situ Large-Scale Construction:Quantitative Evaluation of Regolith Solidification Techniques

Lunar habitat construction is crucial for successful lunar exploration missions.Due to the limitations of transportation conditions,extensive global research has been conducted on lunar in situ material processing techniques in recent years.The aim of this paper is to provide a comprehensive review,precise classification,and quantitative evaluation of these approaches,focusing specifically on four main approaches:reaction solidification(RS),sintering/melting(SM),bonding solidification(BS),and confinement formation(CF).Eight key indicators have been identified for the construction of low-cost and highperformance systems to assess the feasibility of these methods:in situ material ratio,curing temperature,curing time,implementation conditions,compressive strength,tensile strength,curing dimensions,and environmental adaptability.The scoring thresholds are determined by comparing the construction requirements with the actual capabilities.Among the evaluated methods,regolith bagging has emerged as a promising option due to its high in situ material ratio,low time requirement,lack of hightemperature requirements,and minimal shortcomings,with only the compressive strength falling below the neutral score.The compressive strength still maintains a value of 2–3 MPa.The proposed construction scheme utilizing regolith bags offers numerous advantages,including rapid and large-scale construction,ensured tensile strength,and reduced reliance on equipment and energy.In this study,guidelines for evaluating regolith solidification techniques are provided,and directions for improvement are offered.The proposed lunar habitat design based on regolith bags is a practical reference for future research.

Analytical investigations of in situ stress inversion from borehole breakout geometries

This study aims to investigate the feasibility of deriving in situ horizontal stresses from the breakout width and depth using the analytical method.Twenty-three breakout data with different borehole sizes were collected and three failure criteria were studied.Based on the Kirsch equations,relatively accurate major horizontal stress(sH)estimations from known minor horizontal stress(sh)were achieved with percentage errors ranging from 0.33%to 44.08%using the breakout width.The Mogi-Coulomb failure criterion(average error:13.1%)outperformed modified Wiebols-Cook(average error:19.09%)and modified Lade(average error:18.09%)failure criteria.However,none of the tested constitutive models could yield reasonable sh predictions from known sH using the same approach due to the analytical expression of the redistributed stress and the nature of the constitutive models.In consideration of this issue,the horizontal stress ratio(sH/sh)is suggested as an alternative input,which could estimate both sH and sh with the same level of accuracy.Moreover,the estimation accuracies for both large-scale and laboratory-scale breakouts are comparable,suggesting the applicability of this approach across different breakout sizes.For breakout depth,conformal mapping and complex variable method were used to calculate the stress concentration around the breakout tip,allowing the expression of redistributed stresses using binomials composed of sH and sh.Nevertheless,analysis of the breakout depth stabilisation mechanism indicates that additional parameters are required to utilise normalised breakout depth for stress estimation compared to breakout width.These parameters are challenging to obtain,especially under field conditions,meaning utilising normalised breakout depth analytically in practical applications faces significant challenges and remains infeasible at this stage.Nonetheless,the normalised breakout depth should still be considered a critical input for any empirical and statistical stress estimation method given its significant correlation with horizontal stresses.The outcome of this paper is expected to contribute valuable insights into the breakout stabilisation mechanisms and estimation of in situ stress magnitudes based on borehole breakout geometries.

Extended finite element-based cohesive zone method for modeling simultaneous hydraulic fracture height growth in layered reservoirs

In this study,a fully coupled hydromechanical model within the extended finite element method(XFEM)-based cohesive zone method(CZM)is employed to investigate the simultaneous height growth behavior of multi-cluster hydraulic fractures in layered porous reservoirs with modulus contrast.The coupled hydromechanical model is first verified against an analytical solution and a laboratory experiment.Then,the fracture geometry(e.g.height,aperture,and area)and fluid pressure evolutions of multiple hydraulic fractures placed in a porous reservoir interbedded with alternating stiff and soft layers are investigated using the model.The stress and pore pressure distributions within the layered reservoir during fluid injection are also presented.The simulation results reveal that stress umbrellas are easily to form among multiple hydraulic fractures’tips when propagating in soft layers,which impedes the simultaneous height growth.It is also observed that the impediment effect of soft layer is much more significant in the fractures suppressed by the preferential growth of adjoining fractures.After that,the combined effect of in situ stress ratio and fracturing spacing on the multi-fracture height growth is presented,and the results elucidate the influence of in situ stress ratio on the height growth behavior depending on the fracture spacing.Finally,it is found that the inclusion of soft layers changes the aperture distribution of outmost and interior hydraulic fractures.The results obtained from this study may provide some insights on the understanding of hydraulic fracture height containment observed in filed.

Preparation of Al-Al_3Ti in situ composites by direct reaction method

Al-Al3Ti composites were prepared by a direct reaction method, in which Al3Ti was formed by the reaction of Ti and Al in aluminum alloy melt. The morphology of Al3Ti changes apparently from the fine particle, needle-like to large block with the increase of Al3Ti content. The addition of magnesium can markedly change the morphology of Al3Ti and reduce their size. Short rod-like Al3Ti was formed and homogeneous distribution was obtained with the addition of 3 wt.% Mg. The effect of Al3Ti and Mg on the microstructure of Al-Al3Ti composites and the mechanism were also discussed.

Clinical role and importance of fluorescence in situ hybridization method in diagnosis of H pylori infection and determination of clarithromycin resistance in H pylori eradication therapy

H pylori is etiologically associated with gastritis, gas-tric and duodenal ulcers, gastric adenocarcinoma and mucosa-associated lymphoid tissue (MALT) lymphoma. Eradicating H pylori may convert rapidly the outcome of related diseases with the use of more accurate diagnostic molecular tests. Indeed some of the tests cannot give the evidence of current infection; H pylori can be detected by noninvasive and invasive methods, the latter requiring an endoscopy. Eradication failure is a big problem in H pylori infection. Recently, clarithromycin resistance in H pylori strains is increasing and eradicati-on therapy of this bacterium is becoming more difficult. Molecular methods have frequently been applied besides phenotypic methods for susceptibility testing to detect clarithromycin resistance due to mutations in the 2143 and 2144 positions of 23S rRNA gene. Fluorescence in situ hybridization (FISH) method on paraffin embedded tissue is a rapid, accurate and cost-effective method for the detection of H pylori infection and to determine clarithromycin resistance within three hours according to the gold standards as a non-culture method. This method can also be applied to fresh biopsy samples and the isolated colonies from a culture of H pylori, detecting both the culturable bacillary forms and the coccoid forms of H pylori, besides the paraffin embedded tissue secti-ons. This technique is helpful for determining the bac-terial density and the results of treatment where clarith-romycin has been widely used in populations to increase the efficacy of the treatment and to clarify the treatment failure in vitro.

Analysis on method for effective in-situ stress measurement in hot dry rock reservoir

With the rapid increase of energy demand and the increasingly highlighted environmental problems, clean, safe and widely distributed geothermal resources have become a hot spot for renewable resources development. The state of in-situ stress is a major control parameter for multiple links including well location, fracture inspiration and reservoir assessment, so how to determine the accurate state of in-situ stress in the deep thermal reservoir becomes a core problem drawing widely attention and urgent to be solved. Based on features of hot dry rock reservoir in terms of temperature and pressure and the comparison analysis, this article proposes the method of Anelastic Strain Recovery(ASR) as an effective method for determining the state of in-situ stress in the area with HDR resources distributed and explains the availability of ASR method by application examples.

Research of in-situ hydraulic test method by using double packer equipment

Double packer equipment for hydraulic test can be used to measure pressure of test zone directly, and it is frequently used to perform many kinds of hydraulic tests and take groundwater sample from borehole. The test method of this equipment mainly includes the test design, implementation, interpretation and synthetic analysis. By adopting the double packer equipment for hydraulic test, the parameter distribution of rock permeability along borehole can be acquired, as well as the connectivity, water conductivity and water bearing capacity of the disclosed structure and the chemical characteristics of the deep groundwater. It is a necessary method for the research and evaluation of the complex hypotonicity terrace site selection under geological conditions. This method is not only suitable for the geological disposal of high level radioactive waste, but also can be used in the site selection of underground facilities such as storage of petroleum and carbon dioxide. Meanwhile, it has a good application prospect in other hydrogeological investigation fields.

ACTIVATION OF NUCLEOLAR rRNA GENE TRANSCRIPTION IN LYMPHOCYTES BY TUMOR PROMOTERS: APPLIES STUDIES WITH IN SITU HYBRIDIZATION DETECTED BY FLUORESCENT METHOD

The distribution of rDNA was visualized in interphase nuclei of tumor promoter treated human lymphocytes in comparison with the mltogen Phytoheamagglutinin (PHA) effects by using an in situ hybridization fluorescent method. The procedure involves biotinylated rDNA as the probe and FITC-avidin as detection system. Silver (Ag) staining was used to visualize nucleoli. In the interphase nuclei of most of the nonstimulated control lymphocytes, only one small Ag-stained nucleolus could be seen. The in situ hybridization, however, revealed one to several agglomerations of rDNA fluorescent spots. With tumor promoting herb extract WCE (40 μg/ml) or TPA (60 ng/ ml) treatment, the Interphase nucleoli increased slightly in number with the morphology alteration into larger, reticular or compact granular types. Ag-stained particles also increased in number. The number of the in situ hybridization rDNA fluorescent spots and dots increased markedly and largereticulate formations of numerous rDNA spots were seen. This phenomenum resembles to the changes in PHA stimulated lymphocytes. Statistic analysized data showed signifcant difference between control and drug- treated cells. These results indicate that transcriptionally activated rDNA and amplification of total rDNA was induced by the used tumor promoters.

Optimization of construction machinery scheme of cast-in-situ concrete based on the improved scatter degree method

The basic requirement of mechanical construction of cast-in-situ concrete is that it could not only conduct quality qualification and safety production, but also achieve most economic benefits with less investment under the condition to meet the needs of project duration. Therefore, the selection of construction machinery scheme plays an important role. However, in the actual construction, it is usually that operators rely on their own experience and field conditions to determine the mechanics. Such a method is subjective and arbitrary, and it is not conducive to make the construction rationally. Considering the above reasons, an improved weight coefficient method was used to establish an estimation model to estimate the construction machinery scheme of cast-in-situ concrete, so as to make the procedure much rational.

Gene transformation by in situ pistil delivery method in Brassica

Agrobacterium-mediated gene transformation in Brassica crops is a time-con-suming and labor-cost method （tissue culture and plant regeneration） in a genotype-depen-dent way. Here we described a new method 7n situ pistil delivery （ISPDy method with no tissue culture and adaptive to three Brassica species. Briefly this method can be stated as placement of a liquid drop containing binary vector DNA onto the cut surface of emasculated and artificial pollinated Brassica stigma. Transformation rate （Basta-resistant seedlings per 100 pistils） of different species ranged from 0.4% to 16.3%. Factors affecting transforma-tion success included tinning and buffer type. PCR and Southern blot confirmed transgenic events. Reciprocal cross showed reporter gene steadily inherited in nuclear transformed pattern. GUS histochemical assay of transgenic immature seeds showed stained embroys and endosperm in the same seed, indicating DNA delivered through style and integrated into Brassica seed/genome after double fertilization.

In-situ Characterization of Non-aqueous Nano-dispersion Systems by Freeze-etching TEM and Comparative Study with Laser Scattering Method

In-situ characterization of non-aqueous nano-dispersion systems（NANDS） by freeze-etching transmission electron microscope（FETEM） was reported.To improve just-for-once successive rate of specimen preparation and get good characterization results,an improving specimen preparation method of freezing etching was developed.Size,distribution and morphology of NANDS were directly visualized.Some information of particle dispersion feature and particle density can also be obtained.Reproductivity of the FETEM characterization is excellent.Comparing with laser scattering method,which is liable to give positive error especially for small size particle anchoring disperser,FETEM characterization can give more accurate measurement of particle size.Moreover,FETEM can give dispersion feature of nanoparticle in non-aqueous medium.

超深勘察钻孔ASR法地应力测试技术应用

宝灵山隧道是某铁路先开段的重难点控制性工程,其中宝灵山DZ-06勘察钻孔深2118.00 m,是该段深度最大的控制性勘察钻孔。在钻孔的2072.53、2084.27 m深度选取合适岩心,采用ASR法(非弹性应变恢复法)开展了地应力测试,并与该钻孔采用水压致裂法获取的地应力结果进行对比,同时验证了ASR法对于估测超深钻孔的三维地应力大小和方向的实用价值。结果表明:在钻孔2072.53~2084.27 m深度范围,ASR法2个测点获取的最大主应力、中间主应力、最小主应力平均值分别为59.70、54.03、30.74 MPa,最大主应力近水平,方向近SN向,说明该区域以水平构造应力为主;ASR法的最大主应力测试结果均值与水压致裂法测试结果回归值一致性很好,最小主应力测试结果均值与水压致裂法测试结果回归值的一致性处于合理范围,两种方法的水平主应力方向测试结果基本一致。

重复注气压降法煤层渗透率模型与原位测试研究

煤层渗透率作为衡量瓦斯渗流与抽采难易程度的重要指标,对其进行准确测定具有重要意义。针对现有方法计算渗透率测试周期长、结果不稳定、模型不完善等问题,研究煤层渗透率的快速准确测定方法及相应的计算模型。基于煤层中气体径向不稳定流控制方程,结合不同压差下气体在煤层中的体积流量方程,建立可利用全区间压降数据测定煤层渗透率的注气压降计算模型。应用COMSOL数值模拟软件的达西渗流模块对模型进行求解,针对现场工程设计中可对压降曲线产生影响的测压气室长度进行单变量处理,根据模拟结果分析钻孔的测压气室长度可设计为2.0 m。根据数值模拟结果进行现场布置,搭建井下重复注气压降试验系统,结合煤层瓦斯赋存条件和巷道条件施工两组穿层钻孔,对2个测点分别注入两次高于煤层瓦斯压力的补偿气体进行渗透率原位测试,测试周期分别约为6 d和17 d,第2轮测试的注气压力高于第1轮。结合理论推导验证了注气压降过程中煤层瓦斯的雷诺数均处于线性达西渗流段,瓦斯在煤层中的渗流符合达西定律,满足计算模型的假设。与传统煤层渗透率计算方法进行了比较,结果表明:本方法和径向流量法的计算结果基本一致,可以满足实际工程需要。重复注气压降法的测试结果稳定可靠,具备快速测定的优点。

钻孔剪切力学参数表征修正方法及其实验研究

钻孔剪切法是现场快速实测岩石抗剪强度的重要方法,但因测量误差较大导致其应用受限。通过对钻孔剪切原理及研究成果分析,发现了不完全剪切是岩石钻孔剪切试验误差大的原因,提出了基于压、剪不等面积的钻孔剪切法修正方法。设计实验装置开展钻孔剪切模拟试验,研究了不同剪切板参数下砂岩剪切力学参数表征规律,证明了所提方法的有效性和修正效果,最后通过文献数据验证了该方法的适用性。具体结果如下:①岩石钻孔剪切试验误差大的根本原因是受压面积和剪切面积选取失真且不相等,实际剪切破坏面积小于剪切齿齿间面积,且不同剪切齿长度情况下修正受压面积和剪切面积均随法向力的增大线性增大。基于剪切齿参数、受压面积和剪切面积的关系式,提出了钻孔剪切法修正方法。②岩石钻孔剪切的剪应力−剪切位移曲线可分为压密阶段、似弹性变形阶段、塑性变形阶段和峰后阶段。随法向力增大,不同剪切板参数下正应力、剪应力和剪切刚度均增大,达到剪应力峰值时的位移减小。钻孔剪切参数与剪切力学参数表征值之间存在明显规律。随剪切齿齿长的增加,黏聚力线性增大,内摩擦角呈二次函数增大。随剪切板面积的增加,黏聚力线性增大,内摩擦角呈二次函数减小。③剪切齿齿长和剪切板面积增大可在一定程度上减少测量偏差。剪切齿齿长从1.0 mm增大到2.5 mm,黏聚力和内摩擦角测量值的总偏差从62.96%降低到19.91%,总修正提升值从30.8%提高到74.1%;剪切板面积从400 mm^(2)增大到520 mm^(2),黏聚力和内摩擦角测量值的总偏差从64.07%降低到37.69%,总修正提升值从39.56%提高到56.05%。实验和文献数据验证均表明,本修正方法可以使钻孔剪切法测得的黏聚力和内摩擦角准确度分别提升约50%和6%。

雅鲁藏布江断裂带东段现今地应力测量与断层活动性分析

为获取位于雅鲁藏布江断裂带东段加查县新建水电站工程场区的地应力状态,探讨雅鲁藏布江断裂带东段活动性,采用自主研发的新一代水压致裂地应力测试技术,实测获取了工程场区的地应力状态,并在收集雅鲁藏布江断裂带沿线地应力数据基础上,结合摩尔-库仑破裂准则,对其断层活动性进行了探讨。结果表明:(1)在工程厂区内122.75~418.75 m深度,实测最大水平主应力SH介于6.07~37.62MPa之间,最小水平主应力Sh介于3.13~20.33MPa之间,最大水平主应力优势方向为北北东向;(2)主应力关系总体表现为SH>Sh>Sv(Sv为垂向主应力),应力结构有利于逆断层的孕育和活动;(3)实测及收集的大部分数据应力莫尔圆与摩擦系数为0.6的破坏临界线相交,表明整体上雅鲁藏布江断裂带东段发生滑动失稳的风险较高,其中西侧贡嘎至朗县一带风险高于东侧林芝地区。

基于CP-DeepLabv3+的玉米根系图像分割

利用微根管技术可以直接监测植物根系动态生长,并获取清晰根系图像,但土壤环境复杂、颗粒不均匀、细根数量多,图像分割时容易造成根系不连续,将土壤背景误认为根系。针对以上问题,提出了CP-DeepLabv3+算法进行图像分割。该算法引入坐标注意力机制(coordinate attention, CA),更精确地获得分割目标信息,使得分割目标边缘更加连续;在ASPP特征提取模块加入条纹池化(strip pooling,SP)分支,避免在相距较远的位置之间建立不必要的连接,提高图像分割精度。利用CP-DeepLabv3+算法对玉米根系数据集进行测试,结果显示,平均交并比(mean intersection over union,MIoU)值为82.95%,平均像素精确度(mean pixel accuracy,MPA)值为92.47%,相比于原始DeepLabv3+模型分别提高了3.69%、4.44%,表明该算法可有效分割玉米根系,对图像特征提取具有实际意义。

首山一矿深部地应力分布规律及影响因素

为探讨平顶山首山一矿深部地应力分布规律及其影响因素,基于矿井实测地应力数据,运用构造物理学、岩石力学和数值模拟方法,研究了测点地应力类型、大小和方向分布规律,反演了地应力场分布特征,分析了埋深、岩性和地质构造对地应力的影响,确定了影响地应力分布的主控因素。研究表明:多元回归反演分析法获得的地应力计算值与实测值吻合,相对误差小于20%,结果可靠。矿井煤岩体处于三维压应力状态,煤层主应力关系为S_(H)>S_(V)>S_(h),地应力由矿井北部向南部呈现先增大后减小再增大的变化趋势,地应力为28~44 MPa,属于高应力水平,地应力方向为NEE向。埋深影响地应力大小和类型,随埋深增加主应力增大,应力场类型向准静水压力场过渡;岩性与地应力密切相关,从泥岩到砂岩或灰岩,地应力增大,弹性模量越大地应力越大,地层岩性的差异造成地应力大小离散分布、应力方向偏转角不超过10°;矿井地应力与埋深、弹性模量的关系可表示为σ_(H)=0.0350H+0.4681E−8.5513。地质构造是影响首山一矿地应力分布的主控因素,褶皱形态控制地应力的水平应力分布,向斜内弧应力值大于背斜内弧,且褶皱弯曲程度越大,其内弧地应力越大,应力梯度越大;断层带内应力降低、断层尖灭端应力集中,地应力方向沿着断层走向偏转,与断层走向夹角越大,应力方向偏转角越大;断层切割复式褶皱时,断层与向斜构造组合区应力值大于断层与背斜构造组合区,且断褶构造组合造成地应力方向分布紊乱。

生态文明研究方法论:变焦与在场

“生态文明”已经成为一个学术热词,这既是好事也值得警惕。人文学者从事生态文明、生物多样性研究,通常视角较为单一,两手干干净净,只是坐而论道。从方法论上看,对于前者,需要考虑“变焦方法”,即在不同的尺度上考察问题。对于后者,人文学者宜深入实际,先弄脏自己,多进行“在场哲学”研究。中国有一系列值得关注的生态、环境、生物多样性破坏案例,人文学者可对其进行有益的分析。“迁地保护”事件依然值得追踪,它是科学界、园艺界长期惯性思维的一个突出表现。

基于隧道施工阶段地应力实测数据的岩爆判据研究

云南省蔓金高速草果山隧道属于深埋长隧道,隧道围岩坚硬完整且脆性较高,隧道施工阶段面临着岩爆发生的风险。因此,采用应力解除法进行地应力测量,并基于实测数据结合现场岩爆发生情况与国内外不同的强度判据进行对比,并对岩爆判据的适用性进行分析研究。最后,根据岩爆实际发生的情况和位置对岩爆判据进行验证分析,结果表明:岩爆预测标准除应力指标外,还应使用多因素指标进行综合判别;某种岩爆预测方法不能适用所有地下工程,应结合工程的具体情况,进行岩爆危险性评估,为工程提出岩爆防治措施。同时此研究成果为深埋完整硬岩的岩爆灾害分析预测和防控提供了参考依据。