The reductions of cytochrome c by a series of derivatives of Fe(EDTA)2- complex have been studied by a stopped-flow technique. The reactions of cytochrome c with Fe(EDTA)2-, Fe (CDTA)2- and Fe(IDA)22- present typical ...The reductions of cytochrome c by a series of derivatives of Fe(EDTA)2- complex have been studied by a stopped-flow technique. The reactions of cytochrome c with Fe(EDTA)2-, Fe (CDTA)2- and Fe(IDA)22- present typical outer sphere mechanism, meanwhile the cytochrome c(III) and Fe(NTA)- system shows abnormal kinetic behavior, including the rate saturation, big negative entropy and lower overall charge and binding site charge calculated from the dependence of electron transfer rate on ionic strength. On the basis of these observations a semi-inner-sphere mechanism is proposed to illustrate the kinetics.展开更多
基金Project supported by the National Natural Science Foundation of China
文摘The reductions of cytochrome c by a series of derivatives of Fe(EDTA)2- complex have been studied by a stopped-flow technique. The reactions of cytochrome c with Fe(EDTA)2-, Fe (CDTA)2- and Fe(IDA)22- present typical outer sphere mechanism, meanwhile the cytochrome c(III) and Fe(NTA)- system shows abnormal kinetic behavior, including the rate saturation, big negative entropy and lower overall charge and binding site charge calculated from the dependence of electron transfer rate on ionic strength. On the basis of these observations a semi-inner-sphere mechanism is proposed to illustrate the kinetics.
