Prevention and treatment of radiation-induced lung injury by hydroxypiperquin phosphate: a clinical and experimental study

Objective: To evaluate the hydroxypiperquin phosphate (HPQP) as a modifier of radiation-induced injury in human and rat lungs. Methods: Sixty-five patients with lung cancer treated with conventional radiotherapy were divided into 2 groups randomly: Thirty cases were treated with HPQP and the others were in a control group. The changes of X - ray manifestation before, after and during taking drug were compared. An animal model of radiation-induced fibrosis of lungs was also established. Hydroxyproling (HP) content in lung tissue and the pathological changes in rat lungs were checked with microscope and electron microscope after 4 months and 6 months respectively. Results: The changes of lung X-ray manifestation in treatment group were much lighter than that in control group. The HP content and the change of pathology in the lungs of those rats with HPQP treatment were obviously less than that in control group. Conclusion: HPQP plays an important role in prevention and treatment of radiation-induced injury in lungs.

磷酸羟基哌喹对大鼠放射性肺纤维化的防治作用

目的:研究磷酸羟基哌喹(hydroxypiperquin phosphate,HPQP)对大鼠放射性肺纤维化的防治作用。方法:选雄性成年SD大鼠给予0、15 Gy、20 Gy、25 Gy四种剂量60Co γ射线单次半胸照射。各剂量组再分服药(HPQP)组与不服药组(对照组)。照射后4个月和6个月两个时间点对各组的肺组织羟脯氨酸(HP)含量及肺病理形态变化(光镜和电镜)进行研究。结果:照射剂量愈大,肺组织HP含量亦愈高,其中20 Gy、25 Gy组与对照组相比有非常显著差别(P<0.01),而服药组肺HP含量明显低于不服药组(P<0.01)。病理形态变化(肺泡壁增厚、胶原增多、肺泡腔内巨噬细胞和泡沫样细胞出现、肺毛细血管腔狭窄、闭塞等)亦随着照射剂量的加大而愈趋严重,服药组可明显减轻上述改变。结论:证实HPQP可抑制胶原形成和减轻照射所引起的病理变化,对大鼠放射性肺纤维化具有一定的防治作用,并复制了放射性肺纤维化的动物模型。

磷酸羟基哌喹对血液系统辐射损伤保护作用的实验研究

目的研究磷酸羟基哌喹对辐射损伤小鼠血液系统的保护作用。方法40只昆明小鼠,随机分为5组(HPQP低、中、高剂量组,空白对照组和正常组),除正常组外,其余各组给予4.5 Gy60Co-γ射线全身照射,以外周血白细胞、血小板计数观察辐射后小鼠的血液系统变化,初步观察药物对血液系统的影响,并筛选出药物的合适剂量;另取24只小鼠,随机分为3组(用药组、对照组、正常组),以此前确定的磷酸羟基哌喹最适剂量于照射前灌胃给药,予9.0 Gy60Co-γ射线全身照射,照射后第9天处死小鼠,以脾结节法、骨髓有核细胞计数法、骨髓病理观察药物对小鼠造血功能的影响。结果磷酸羟基哌喹可促进辐射后小鼠白细胞恢复(P<0.01),降低死亡率(P<0.01),使脾结节(CFU-S)数、骨髓有核细胞数明显增加(P<0.01)。结论磷酸羟基哌喹对辐射损伤小鼠血液系统有保护作用。