Genetic investigation of the ubiquitin-protein ligase E3A gene as putative target in Angelman syndrome

BACKGROUND Angelman syndrome(AS)is caused by maternal chromosomal deletions,imprinting defects,paternal uniparental disomy involving chromosome 15 and the ubiquitin-protein ligase UBE3A gene mutations.However the genetic basis remains unclear for several patients.AIM To investigate the involvement of UBE3A gene in AS and identifying new potential genes using exome sequencing.METHODS We established a cohort study in 50 patients referred to Farhat Hached University Hospital between 2006 and 2021,with a strong suspicion of AS and absence of chromosomal aberrations.The UBE3A gene was screened for mutation detection.Two unrelated patients issued from consanguineous families were subjected to exome analysis.RESULTS We describe seven UBE3A variants among them 3 none previously described including intronic variants c.2220+14T>C(intron14),c.2507+43T>A(Exon15)and insertion in Exon7:c.30-47_30-46.The exome sequencing revealed 22 potential genes that could be involved in AS-like syndromes that should be investigated further.CONCLUSION Screening for UBE3A mutations in AS patients has been proven to be useful to confirm the diagnosis.Our exome findings could rise to new potential alternative target genes for genetic counseling.

The function of T-lymphocyte subtypes of blood in patients with hyper-IgE syndrome

Objective: To study the function in cellular immunity of patients with hyper-IgE syndrome (HIE). Methods: T-lymphocyte subtypes of the peripheral blood and cutaneous delayed-type hypersensitivity (DTH) to two recall antigens, tetanus toxoid (TT) and purified protein derivative(PPD), were measured in 5 patients with HIE and 15 healthy controls, respectively. Results: The CD4 + cell counts in HIE group were significantly lower than those in the control group (P<0.01). In contrast, CD8 + cells were significantly higher in HIE group than those in the controls. The induration sizes of DTH to two recall antigens were smaller in HIE group than those in controls (P<0.01). Conclusion: There is an immunologic dysfunction of T lymphocytes in the patients with HIE and T cells play an important role in the pathogenesis.

Analysis of T-lymphocyte subtypes of the peripheral blood and skindelayed-type hypersensitivity in patients with hyper-IgE syndrome

Objective: To study the function of ce ll ular immunity in patients with hyper-IgE syndrome (HIE). Methods: T-lymphocyte subtypes of the peripheral blood and cutaneous delayed-typ e hypersensitivity (DTH) response to two recall antigens, tetanus toxoid (TT) an d purified protein derivative(PPD), were measured in five patients with HIE and 15 healthy controls, respectively. Results: The CD4+ cell cou nts in HIE group were significantly lower than those in control group (P<0. 01). In contrast, CD8+ cells were significantly higher than those in the contro l (P<0.01). The induration sizes of DTH response to two recall antigens wer e smaller in HIE group than those in the control group (P<0.01). Co nclusion: There was an immunologic dysfunction of T lymphocytes in the p atients with HIE.

Association of Apolipoprotein E Gene Polymorphism with Lipid Profile in Patients with Acute Coronary Syndrome in Han Chinese: A Critical Review

This review paper focuses on the genetic contribution, in particular, the association of Apolipoprotein E gene polymorphism to lipid abnormality and subsequent acute coronary syndrome in Han Chinese of China. Many researches have been published pertaining the influence of ApoE gene polymorphism on coronary artery disease, dyslipidemia and the response of statin in Han Chinese. Most of the studies in Han Chinese like other populations demonstrated that ApoE 4 allele genetically predisposes coronary artery disease, acute coronary syndrome, severity of occlusion of coronary artery and higher incidence of major adverse cardiovascular events (In Han Chinese, ApoE allele carriers demonstrated 85% increase in major adverse cardiovascular events (MACE) in six months follow up). In addition, ApoE4 allele carrier also showed both increased in LDL level and decrease response to statin therapy in dyslipidemic Han Chinese. On the other hand, ApoE2 carrier is scavenger of cholesterol and triglyceride from the blood;?thus it is cardiovascular-protective. Despite positive relationship between ApoE gene polymorphism and cardiovascular pathologies, prognostic outcome and resistance to intervention, this area of research still requires?extensive investigation in Han Chinese. Because, several other studies revealed either negative effect or showed no effect by ApoE gene polymorphism on cardiovascular disease. Some of the causes of such debatable results could be explained by factors such as diminutive frequency allele and expression of ApoE gene in coronary heart disease. This part of the research yet requires extensive study with bulkier sample size and retrospective in nature, in order to ascertain the influence of ApoE genotype on lipid, anti-hyperlipidemic agent and coronary heart disease. Such studies could assist us to confirm whether to test healthier subjects to predict genetic risk of coronary heart disease in Han Chinese population. The aim of this review paper is to critically analyze the effect of ApoE gene on the occurrence of coronary heart disease in Han Chinese.

Guillain-Barrésyndrome following hepatitis E

Guillain-Barrésyndrome(GBS)is often triggered by a preceding bacterial or viral infection.Occasionally,it has been observed in association with acute hepatitis A,B and C,and three cases have been previously described in India in which GBS was associated with acute hepatitis E.A molecular mimicry mechanism is supposed to be involved in the pathogenesis of GBS triggered by infectious agents,although the nature of the shared epitopes has not been characterized in most instances,including that in the case of hepatotropic viruses.We report a case of GBS following acute hepatitis E in a European individual.The presence of antiganglioside GM2 antibodies in this patient suggested molecular mimicry involving ganglioside GM2 in the pathogenesis of GBS associated with hepatitis E.

Effects of Soy Isoflavones on the Sex Hormone Level and Pathological Changes in the Perimenopausal Syndrome Animal Model

[Objectives] To observe the effect of soy isoflavones on the perimenopausal syndrome model of female rats,and to find its mechanism,in order to provide experimental evidence for the clinical treatment of menopausal syndrome. [Methods]50 female SD rats were randomly divided into five groups,with 10 rats in each group,respectively,for the normal control group,sham operation group,model group,diethylstilbestrol group,soy isoflavone treated group,except the normal control group and sham operation group,the other groups were ovariectomized rats,the formation model of perimenopausal rats treated with normal saline,diethylstilbestrol,soy isoflavone by gavage,normal control group and sham operation group were given the same dose of normal saline and soy isoflavone by gavage. After 6 weeks of continuous treatment,abdominal aorta was taken blood,the serum was isolated,and radioimmunoassay method to measure E_2( Eestradiol),P( Progesterone),T( Testosterone). And removal of the pituitary gland,uterine and adrenal prepared pathological section,experimental effect was observed by light microscopy,using immunohistochemical method to observe the brain pituitary FSH( Follicle-Stimulating Hormone) and LH( Luteinizing Hormone) levels. [Results] Radiation immune analysis results showed that soy isoflavone could significantly improve serum E_2 level in ovariectomized rats,compared with the model group,the statistical analysis showed significant difference( P < 0. 05),but the serum P and T level had little effect. The pathological results showed that( i) uterus: the uterine wall and endometrial thickness of soy isoflavone group was between that of model group and sham operation group,the surface epithelium was columnar,glands increased;( ii) pituitary gland: soy isoflavone ovariectomized group cells were between model group and normal group;( iii) adrenal gland: soy isoflavone group adrenal tissue did not change significantly. Immunohistochemical results showed that soy isoflavone group pituitary tissues of FSH and LH cells compared with the model group decreased significantly. [Conclusions] The soy isoflavones could make the uterus wall and endometria of perimenopausal syndrome animal model thicken,improve the serum level of E_2 in ovariectomized rats,and decrease the level of FSH and LH,but had little effect on the adrenal tissues,level of T and P.

Assessment of antioxidant status of women with polycystic ovarian syndrome

Objective:To determine the antioxidant status of females with polycystic ovarian syndrome.Methods:Blood samples of 85 females(45 newly diagnosed polycystic ovarian syndrome patients and 40 apparently healthy subjects)between 25 and 45 years of age were obtained from Ekiti State University Teaching Hospital,Ado Ekiti,Ekiti State,Nigeria.Spectrophotometry was used to evaluate the plasma concentrations of malondialdehyde,vitamin A,C and E,reduced glutathione and activities of glutathione peroxidase,catalase and superoxide dismutase.Results:The concentrations of malondialdehyde and glutathione peroxidase activity significantly increased(P<0.05)in patients with polycystic ovarian syndrome compared with the healthy controls,while concentrations of reduced glutathione,vitamins A,C,E and activities of catalase and superoxide dismutase of patients with polycystic ovarian syndrome showed significant decrease(P<0.05)compared to the healthy controls.This study showed that oxidative stress may assume a role in the pathogenesis of polycystic ovarian syndrome.There were significant negative correlations between malondialdehyde and superoxide dismutase,catalase(P<0.01)and vitamin A(P<0.05),while there was a significant positive correlation(P<0.01)between malondialdehyde and glutathione peroxidase.In addition,vitamins A(P<0.05),C(P<0.01)and E(P<0.01)showed significant positive correlations with catalase antioxidant enzyme.However,vitamins C and E showed significant positive correlation(P<0.05)among each other.Conclusions:The involvement of antioxidants in the management of polycystic ovarian syndrome may be helpful as secondary therapy to prevent oxidative damage and may be used as a potential approach to overcome metabolic as well as reproductive disorders associated with infertility in polycystic ovarian syndrome.

The Study of Apolipoprotein E4 Allele Distribution in Parents of Down’s Syndrome Children as a Risk Factor in Khorasan Razavi Province, Iran

Backgrounds: Down syndrome (DS) is the most common chromosomal abnormality. The most important factor in DS is increased maternal age so after the age of 35, the risk of Down syndrome in pregnancy increases. Down syndrome can be diagnosed during pregnancy by prenatal screening. Nondisjunction in cell divisions is the main cause of the DS. Apo lipoprotein E is a 317 amino acid glycoprotein that plays an essential role in metabolism and cholesterol transport. Alzheimer’s disease (AD) is one of the symptoms of adults with DS. The apoE allele e4 has been identified as a risk factor for AD and also, played a main role in nondisjunction. An increased risk of AD in mothers of adults with DS has been reported. We hypothesized that young mothers of DS children (Methods: In this case-control study, 33 DS children and their parents were compared in case of age with 90 families without any history of DS. Genotyping was performed by ARMS-PCR technique. Statistical analysis was performed by SPSS v.21 software. Results: It indicated that there is a significant difference in allele distribution between case and control groups. The C allele for 112 codon of APOE gene and the C allele for 158 codon of APOE gene may associate with nondisjunction. In 112 codon of APOE gene, it seems having T allele reduces the risk of nondisjunction and in contrast C allele may be a risk factor in happening of nondisjunction. (p-value = 0.000006, OR = 2.66, 95% CI = 1.74 - 4.06). In 158 codon of APOE gene, it seems having T allele reduces the risk of nondisjunction and in contrast C allele may be a risk factor in happening of nondisjunction. (p-value = 0.0000, OR = 3.89, 95% CI = 2.38 - 6.34). E4 allele frequency in mothers of DS is about 14% more than those in control group. According to results of this study the C allele in 158 codon of APOE gene and the C allele in 112 codon of APOE gene could be considered as susceptibility genetic factors for nondisjunction in Northeast of Iran.

维生素E对围绝经期和绝经后妇女健康的影响

随着年龄的增长,卵巢功能由旺盛期到逐渐减退,直至衰竭,女性也将从生殖期逐渐进入老年期。这不仅会对月经周期和生育能力有影响,还会伴随着一系列身体和心理上的变化,严重时会影响生活和工作。研究发现卵巢卵泡的发育以及绝经前后出现的泌尿生殖功能异常、骨质疏松、神经认知功能障碍及心血管病变等疾病的发生发展可能与氧化应激反应有着密不可分的关系。维生素E具有抗氧化特性和稳定细胞的能力,已经被证实可在代谢综合征、骨质疏松、心脑血管、神经系统等疾病的预防和治疗方面发挥着重要作用,并且发现维生素E在一定程度上还具有模拟雌激素的作用。因此,补充维生素E对围绝经期和绝经后妇女的健康具有潜在的益处。综述维生素E对围绝经期卵巢卵泡发育、雌激素的影响和绝经综合征的预防和治疗作用,为维生素E作为改善围绝经期和绝经后妇女身心健康的辅助或替代治疗方案提供参考依据。

载脂蛋白E基因多态性与急性冠状动脉综合征关系的研究进展

急性冠状动脉综合征(ACS)是由不稳定的冠状动脉粥样硬化斑块(AP)破裂或糜烂导致血栓形成而引发的一系列缺血性疾病,其病理基础为AP形成,这一过程受到脂质代谢紊乱的驱动。血脂水平是ACS预后的关键因素,载脂蛋白E(ApoE)作为脂质代谢的关键调控因子,其多态性与血脂异常密切相关。而ApoE4基因型患者ACS后强化他汀治疗效果不佳是ACS的独立危险因素。未来明确ApoE基因多态性与ACS的关系,有助于为ACS患者制订个体化降脂方案提供更为精准、安全的策略。

柴郁清肝汤治疗非酒精性脂肪性肝病肝郁脾虚证患者的疗效观察及对Th17/Treg和相关炎症因子的影响

目的:观察柴郁清肝汤治疗非酒精性脂肪性肝病(NAFLD)肝郁脾虚证患者的临床疗效及对外周血Th17/Treg和相关炎症因子的影响。方法:将60例NAFLD患者随机分为观察组(30例)和对照组(30例),其中观察组给予柴郁清肝汤治疗,对照组给予维生素E软胶囊治疗,治疗8周评价疗效;分别观察2组患者治疗前后肝功、血脂、外周血T淋巴细胞Th17、Treg的百分比及其相关炎症因子(IL-17、IL-22、IL-10)的变化。结果:治疗8周,观察组显效16例,有效12例,对照组显效8例,有效13例,2组比较差异具有统计学意义(P<0.05)。与治疗前比较,2组患者ALT、AST、GGT、ALP、TC、TG、LDL-C、Th17、IL-17和IL-22明显降低,Treg明显升高,差异具有统计学意义(P<0.05),2组间比较观察组均明显优于对照组(P<0.05)。结论:柴郁清肝汤能够有效治疗NAFLD患者,改善肝功能和血脂,调节Th17/Treg平衡及其相关炎性因子分泌是其作用机制之一。

Current Approach to Treatment of Juvenile Idiopathic Arthritis: Case Report of Hiperimmunglobulin E Syndrome Developed Juvenile Idiopathic Arthritis

Introduction: Juvenile idiopathic arthritis (JIA) represents a heterogeneous group of childhood chronic arthritic conditions. The pathogenesis of JIA remains incompletely understood. This disease can lead to a significant morbidity including joint deformity, growth impairment and a persistence of active arthritis into adulthood. The past two decades have witnessed significant advances in treatment and improved outcomes for affected children. With the current use of biologics, more target-specific, better tolerated, safer and more effective treatments have become possible. However, continuing, comprehensive follow-up is needed to characterize the long-term effects of such treatments. Hyperimmunoglobulin E syndrome (hyper-IgE, or Job’s syndrome) is a rare immune deficiency characterized by high IgE levels, atopic chronic eczema, tendency towards re-current pyogenic infection, neutrophil chemotaxis disorder and varying T-cell function impairment. Case Report: The case of a 17-year-old male patient with hyper-IgE who develops the oligoarticular subtype of JIA over a period of four years is discussed. The course of JIA is unfavorable, causing severe deformity of numerous joints (left elbow, right 3rd metacarpophalangeal, left knee, right ankle) and a fungal infection scar on the left eye. Blood tests show an ESR of 89 mm/h, rheumatoid factor (RF) 8.3 IU/mL (0 - 20) and positive antinuclear antibody (ANA). To improve gait, corrective surgery is performed on the right ankle, followed by rehabilitation and physical therapy. Conclusion: Developments in the near future will be crucial for understanding JIA pathophysiology and improving treatment.

血清微小RNA-199a-3p、E盒结合锌指蛋白1水平对急性冠状动脉综合征患者经皮冠状动脉介入术后预后的预测价值

目的探讨微小RNA-199a-3p(miR-199a-3p)、E盒结合锌指蛋白1(ZEB1)对急性冠状动脉综合征(ACS)患者经皮冠状动脉介入(PCI)术后预后的预测价值。方法选取2021年5月至2022年5月秦皇岛市第二医院接收的行PCI术治疗的113例ACS患者为观察组,根据观察组患者PCI术后1年内是否发生主要不良心血管事件(MACE),分为MACE组26例和非MACE组87例,同期健康体检者106例为对照组。检测血清miR-199a-3p、ZEB1水平,比较MACE组和非MACE组临床资料,Pearson分析患者血清miR-199a-3p和ZEB1水平相关性,多因素Logistic分析影响ACS患者PCI术后发生MACE的影响因素,ROC曲线分析血清miR-199a-3p、ZEB1对ACS患者PCI术后发生MACE的预测价值。结果与对照组相比,观察组血清miR-199a-3p水平较低,ZEB1水平较高(t=13.709、25.641,P<0.05);在ACS患者中miR-199a-3p与ZEB1呈负相关(r=-0.421,P<0.05);ACS患者PCI术后较PCI术前血清miR-199a-3p水平高,ZEB1水平低(t=4.820、6.040,P<0.05);MACE组与非MACE组高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、左心室射血分数、miR-199a-3p、ZEB1比较,差异有统计学意义(t=3.310、2.717、3.947、7.068、6.544,P<0.05);LDL-C、ZEB1水平是ACS患者PCI术后MACE的危险因素,左心室射血分数、HDL-C、miR-199a-3p水平是ACS患者PCI术后MACE的保护因素;miR-199a-3p、ZEB1联合预测ACS患者PCI术后MACE的曲线下面积为0.947,优于单独预测(Z=1.970、2.791,P<0.05)。结论ACS患者血清中miR-199a-3p、ZEB1水平呈负相关,两者均对ACS患者PCI术后MACE有预测价值,两者联合的预测价值更高。

载脂蛋白E基因多态性与原发性高脂血症中医辨证的相关性研究

目的探讨载脂蛋白E(ApoE)基因多态性与原发性高脂血症中医辨证的相关关系。方法采用基因测序法检测102例高脂血症患者的ApoE基因型。结果共检出5种基因型,分别是E2/2、E3/3、E4/4、E2/3、E3/4;肝肾阴虚证E3/4+E4/4基因型频率和ε4等位基因频率明显高于脾肾阳虚证和痰浊阻遏证(P<0·05,P<0·01),气滞血瘀证E3/4+E4/4基因型频率和ε4等位基因频率也明显高于痰浊阻遏证(P<0·05)。结论ApoE基因多态性与原发性高脂血症中医辨证具有一定的相关性。

前列腺素E1对原发性肾病综合征作用初探(英文)

Objective:To investigate the effect of prostaglandin E1 (PGE1) (Alprostadii injection) on patients with primary nephrotic syndrome(PNS). Methods: 37 patients with PNS were recruited to study the effect of prostaglandin E1 on platelet aggregation function [ PAG (5,) PAG( m ) ], serum total protein (TP) , albumin (Al),blood urea nitrogen(BUN) ,serum creatinine(Scr) ,cholesterol(CHO), triglyceride(TG), protein in 24-hour urine (Pr/24h) and platelet account (PLT). Results: TP, Al, CHO, TG, BUN, Scr, Pr/24h, PAG(5) and PAG(m) in PNS group before treatment were significantly different from those in control group(P＜0.05, P＜0.01) while no significant difference was found for PLT. When treated with PGE1 , TP,Al,CHO, TG, Pr/24h, ADP- induced PAG(5) ,and Adr- induced PAG(5) and PAG(m) were significantly different from those before treatment (P＜0.05). Adr- induced PAG(5) and PAG(m) were significantly different. Adr- induced PAG(5) was xsitively correlated with BUN and Scr in PNS(P＜0.01). Similar correlation was found between ADP-induced PAG(5) and Al ,BUN,Scr,Pr/24h(P＜0.05), AD- induced PAG(m) and TP,CHO(P＜0.05). Conclusions: PGE1 may be an effective drug for the treatment for hypercoagulation in patients with PNS.

载脂蛋白E基因多态性与高脂血症痰瘀证患者血脂水平关系的研究

目的:研究载脂蛋白E(ApoE)基因多态性对高脂血症痰瘀证患者血脂水平的影响。方法:运用PCR-RFLP法检测210例高脂血症痰瘀证患者ApoE基因分型,分析ApoE基因多态性与血脂总胆固醇(TC)、甘油三酯(TG)、低密度胆固醇(LDL-C)、高密度胆固醇(HDL-C)水平的关系,结果:高脂血症痰瘀证患者不同ApoE基因型,血脂LDL-C水平,按照E3/4﹥E3/3﹥E2/3的规律逐渐降低,经比较具有显著性差异,P<0.01(P=0.0046),以E3/4为最高,E2/3为最低,而TC、TG、HDL-C水平无明显差异。结论:ApoE基因多态性影响高脂血症血脂水平,E4基因型痰瘀证个体血脂LDL-C水平明显增高,推测E4基因型可能是高脂血症痰瘀证候主要易感基因之一。

前列腺素E_1对内毒素致家兔急性肺损伤的治疗效应

本实验以一次性静注大肠杆菌内毒素（ｅｎｄｏｔｏｘｉｎ，ＥＴ，７０μｇ／ｋｇ）方法复制家兔急性肺损伤模型，在实验过程中观察动物血压、血气变化及肺组织病理学改变，结果前列腺索Ｅ＿１（ｐｒｏｓｔａ－ｇａｎｄｉｎＥ＿１，ＰＧＥ＿１）治疗组轻于发病组，采用ＰＧＥ＿１治疗内毒素所致的急性肺损伤有一定疗效。

冠心病痰瘀证与载脂蛋白E基因多态性关系的研究

目的:探讨冠心病(coronary heart disease,CHD)不同痰瘀证候与载脂蛋白E(apolipoprotein E,ApoE)基因第4外显子多态性的关系。方法:选择符合条件的CHD痰证、瘀证、痰瘀互阻证和非痰非瘀证(其他证型)患者200例,另选100名健康志愿者作为对照。常规检测所有样品的血脂水平。提取全血DNA,用聚合酶链反应-限制性片段长度多态性(polymerase chain reaction-restriction fragment length poly-morphism,PCR-RFLP)技术检测ApoE基因型。采用SAS软件对基因多态性和痰瘀证候的辨证类型进行相关性分析。结果:(1)冠心病患者中ε4等位基因频率(19.5%)明显高于健康对照组(9.5%),具有统计学意义(P<0.05),其中尤以E 3/4基因型更为多见(P<0.01)。(2)携带ε4等位基因者的总胆固醇(totalcholesterol,TC)、总甘油三酯(total triglycerides,TG)和低密度脂蛋白胆固醇(low-density lipoproteincholesterol,LDL-C)水平明显高于非携带者(P<0.01)。(3)痰证特别是E3/4基因型患者中E3/4型和ε4等位基因的频率明显高于血瘀证患者(P<0.05)。结论:ApoEε4等位基因是冠心病的危险因素,携带此基因的患者与痰证关系较为密切,推测其可能是CHD痰证的主要易感基因之一。

日粮添加V_E和V_C对肺动脉高压综合征患鸡自由基代谢的影响

将 38 0只 AA商品肉鸡随机分为 A组 10 0只 ,B、C、D和 E组各 70只 ,14日龄前常规饲养。14日龄后 ,B、C、D和E组舍温按每日 1～ 2℃由 2 5℃逐步降至 12℃ ,同时日粮中按 1.5 mg/ kg的剂量添加 T3以诱发肺动脉高压综合征(PHS) ;C、D组在日粮中分别按 5 0 0、10 0 mg/ kg的剂量添加维生素 C和 E;而 E组同时添加维生素 C和 E,A组仍常规饲养 ,至试验结束。记录每周各组鸡群的 PHS发病数、平均体质量和采食量 ,并每周每组取 10只鸡采血和扑杀 ,测定其红细胞压积 (PCV)、血浆、肺和肝组织的超氧化物歧化酶 (SOD)及脂质过氧化物的降解产物丙二醛 (MDA )浓度 ;取心脏测定其右心室和全心室质量比 (RV/ TV)。结果显示 ,环境低温和日粮添加 T3极显著增加了肉鸡 PHS的发病率 (P<0 .0 1)。 C、E组的肉鸡 PHS发病率以及血浆、肺和肝组织的 MDA值均极显著降低 (P<0 .0 1) ,血浆、肺和肝组织的 SOD值均极显著增加 (P<0 .0 1) ,但增重、饲料转换率、血液 PCV值和心脏指数 RV/ TV值未发生改变 ;D组 5周龄后的血浆 MDA值则极显著降低 (P<0 .0 1)以及血浆、肺和肝组的 SOD值极显著增加 (P<0 .0 1) ,而肺和肝组织的 MDA值和肉鸡 PHS发病率未发生改变。由此表明 ,日粮添加维生素 C明显阻断了低温和 T3条件下肉鸡体内脂质过氧?

胃癌及慢性胃病患者脾证分型与cyclinE表达的关系

目的探索胃癌、慢性胃病患者脾证分型与 cyclin E 表达的关系.方法在胃癌和慢性胃病患者中,选取具有中医脾虚表现的脾气(阳)虚、脾阴虚患者,和具有脾实表现的脾胃湿热、寒湿困脾证患者,以及按慢性浅表性胃炎、肠上皮化生、不典型增生、胃癌分组的患者,进行胃粘膜组织 cyclin E 免疫组化染色;对各证型的 cyclin E 表达进行比较,并分析 cyclin E 在常见胄粘膜病变中表达的差异.结果 cyclin E 在上述四种脾证分型的表达有显著性差异,阳性百分率分别为7.9%,27.3%,31.4%和14.3%.在慢性浅表性胄炎、肠上皮化生、不典型增生和胃癌的表达也有显著性差异,阳性率分别为7.5%,28.6%,37.9%和42.6%,后两者已较接近.结论胃癌和慢性胃病患者中,不同的中医脾证其 cyclin E 表达明显不同,在脾胃湿热和睥阴虚证型中较高,在寒湿困脾和脾气(阳)虚证型中较低.cyclin E 表达随病变由慢性浅表性胃炎、癌前病变到癌而逐渐增高,其与胃癌的发生发展密切相关.