Hypercohones A-C,acylphloroglucinol derivatives with homo-adamantane cores from Hypericum cohaerens

Three new homo-adamantanyl type natural products were derived from polyprenylated polycyclic acylphloroglucinol.Hypercohones A-C(1-3),along with five other known hypercohones(4-8),were isolated from the aerial parts of Hypericum cohaerens.The structures of 1-3 were elucidated on the basis of comprehensive spectroscopic analysis.The inhibitory activities of these isolates against five human cancer cell lines in vitro were tested.

Hypercohones D–G,New Polycyclic Polyprenylated Acylphloroglucinol Type Natural Products from Hypericum cohaerens

Four new polycyclic polyprenylated acylphloroglucinol type metabolites,hypercohones D–G(1–4),along with four known analogues(5–8),were isolated from the aerial parts of Hypericum cohaerens.The structures of these isolates were elucidated by extensive spectroscopic methods.The inhibitory activities of these isolates against five human cancer cell lines in vitro were also tested.

尖萼金丝桃中抗人脐静脉内皮细胞高糖损伤活性组分的筛选及其作用机制

目的筛选抗人脐静脉内皮细胞(HUVEC)高糖损伤的活性物质,并研究其机制。方法建立高糖损伤HUVEC模型,筛选提取自尖萼金丝桃的多个样品;检测已知结构活性样品对高糖损伤HUVEC细胞的增殖活性、细胞凋亡和细胞坏死、超氧化物歧化酶(SOD)活性、活性氧(ROS)及肿瘤坏死因子-α(TNF-α)、白细胞介素-4(IL-4)含量的影响。结果获得1个已知结构的活性样品hypercohone G,其可提高HUVEC细胞的增殖活性,减少细胞凋亡,对细胞坏死无影响;能增加SOD的活性,降低ROS、TNF-α、IL-4的含量。结论尖萼金丝桃中hypercohone G能抗高糖所致HUVEC细胞损伤,其机制可能与提高细胞抗氧化能力,减轻炎症反应,减少细胞凋亡有关。