Relationship between hyperemesis gravidarum and Helicobacter pylori seropositivity

Early pregnancy is commonly characterized by the disturbance of digestive function, characteristically manifested by nausea and vomiting. These symptoms during early pregnancy usually disappear spontaneously after about 12 weeks. In a minority of pregnant women, these symptoms are very severe, and are associated with weight loss, dehydration, acidosis from starvation,

Accuracy of Helicobacter pylori serology in two peptic ulcer populations and in healthy controls

AIM: To estimate the test characteristics of Heli- cobacter pylori (Hpylori) serology and of C14-urea breath test (C14-UBT) in two different peptic ulcer populations and in community controls. Second, the aim was to explore the association between the level of H pylori IgG antibodies and severity of inflammation as to active peptic ulceration in the same populations. METHODS: Vagotomized (n= 83), medically treated peptic ulcer patients (n=73) and one reference group of community controls (n=88) were gastroscoped. H pylori status was determined by histology, bacterial growth, C14-UBT and serology. Based on the updated Sydney System, cumulative scores from biopsies from the prepyloruos, incisura angularis, corpus and fundus were calculated. RESULTS: The prevalence of H pylori infection varied from 70% to 79%. The C14-UBT had high accuracycompared to the serology test. The sensitivity of the serology test was good, but the specificity was low (41%-71%). The association between H pylori IgG antibodies and scores of gastric mucosal inflammation and current or previous peptic ulcer were weak. CONCLUSION: The accuracy of C14-UBT to diagnose H pylori infection was good, and the clinical utility of a negative H pylori serology test was substantial, while the gain in clinical information of a positive test was meagre. Positive H pylori titres could not distinguish between subjects with or those without active peptic ulceration.

Helicobacter pylori infection in pregnant women:Gastrointestinal symptoms and pregnancy-related disorders

Helicobacter pylori(H.Pylori)is a gram-negative,flagellated and spiral-shaped bacterial pathogen that impacts approximately 46%among pregnant women globally and has been associated with various maternal-fetal complications.Iron deficiency anemia,fetal growth restriction,cardiovascular diseases,and insufficient nutrient absorption can be observed in pregnant women,as well as miscarriages and pregnancy-specific hypertensive disease,such as pre-eclampsia.Thus,the evidence supports the influence of H.pylori infection on fetal implantation/placentation failure,and positive strains of the cytotoxin-associated gene A of H.Pylori were reported as the most prevalent in these conditions.However,current knowledge indicates a relationship between this infection and the occurrence of hyperemesis gravidarum,characterized by frequent nausea and vomiting.Regarding the diagnosis of this bacterial infection,non-invasive approaches such as stool antigen test,urea breath test,and serological tests are more accepted during pregnancy,as they are easy to carry out and cost-effective.Finally,the bacteria eradication therapy should consider the risks and benefits for the pregnant woman and her child,with pharmacological intervention depending on the clinical presentation.

Helicobacter pylori and pregnancy-related disorders

Helicobacter pylori(H.pylori)infection is investigated in gastric diseases even during pregnancy.In particular,this Gram-negative bacterium seems to be associated with hyperemesis gravidarum,a severe form of nausea and vomiting during pregnancy.During the last decade,the relationship among H.pylori and several extra-gastric diseases strongly emerged in literature.The correlation among H.pylori infection and pregnancy-related disorders was mainly focused on iron deficiency anemia,thrombocytopenia,fetal malformations,miscarriage,pre-eclampsia and fetal growth restriction.H.pylori infection may have a role in the pathogenesis of various pregnancy-related disorders through different mechanisms:depletion of micronutrients(iron and vitamin B12)in maternal anemia and fetal neural tube defects;local or systemic induction of pro-inflammatory cytokines release and oxidative stress in gastrointestinal disorders and pre-eclampsia;cross-reaction between specific anti-H.pylori antibodies and antigens localized in placental tissue and endothelial cells(preeclampsia,fetal growth restriction,miscarriage).Since H.pylori infection is most likely acquired before pregnancy,it is widely believed that hormonal and immunological changes occurring during pregnancy could activate latent H.pylori with a negative impact not only on maternal health(nutritional deficiency,organ injury,death),but also on the fetus(insufficient growth,malformation,death)and sometime consequences can be observed later in life.Another important issue addressed by investigators was to determine whether it is possible to transmit H.pylori infection from mother to child and whether maternal anti-H.pylori antibodies could prevent infant’s infection.Studies on novel diagnostic and therapeutic methods for H.pylori are no less important,since these are particularly sensitive topics in pregnancy conditions.It could be interesting to study the possible correlation between H.pylori infection and other pregnancy-related diseases of unknown etiology,such as gestational diabetes mellitus,obstetric cholestasis and spontaneous preterm delivery.Since H.pylori infection is treatable,the demonstration of its causative role in pregnancy-related disorders will have important social-economic implications.

Helicobacter pylori infection- recent developments in diagnosis

Considering the recommended indications for Helicobacter pylori(H.pylori)eradication therapy and the broad spectrum of available diagnostic methods,a reliable diagnosis is mandatory both before and after eradication therapy.Only highly accurate tests should be used in clinical practice,and the sensitivity and specificity of an adequate test should exceed 90%.The choice of tests should take into account clinical circumstances,the likelihood ratio of positive and negative tests,the cost-effectiveness of the testing strategy and the availability of the tests.This review concerns some of the most recent developments in diagnostic methods of H.pylori infection,namely the contribution of novel endoscopic evaluation methodologies for the diagnosis of H.pylori infection,such as magnifying endoscopy techniques and chromoendoscopy.In addition,the diagnostic contribution of histology and the urea breath test was explored recently in specific clinical settings and patient groups.Recent studies recommend enhancing the number of biopsy fragments for the rapid urease test.Bacterial culture from the gastric biopsy is the gold standard technique,and is recommended for antibiotic susceptibility test.Serology is used for initial screening and the stool antigen test is particularly used when the urea breath test is not available,while molecular methods have gained attention mostly for detecting antibiotic resistance.

Detection of anti-Helicobacter pyloriantibodies in serum and duodenal fluid in peptic gastroduodenal disease

AIM: To study the diagnosis of He/icobacterpy/ori(H pylori) infection through the determination of serum levels of anti-H pylori IgG and IgA antibodies, and the levels of anti- H pylori IgA antibodies in duodenal fluid. METHODS: Data were collected from 93 patients submitted to upper digestive endoscopy due to dyspeptic symptoms. The patients were either negative (group A) or positive (group B) to Hpyloriby means of both histological detection and urease tests. Before endoscopy, peripheral blood was collected for the investigation of anti-H pylori IgG and IgA antibodies. To perform the urease test, biopsies were obtained from the gastric antrum. For the histological evaluation, biopsies were collected from the gastric antrum (greater and lesser curvatures) and the gastric body. Following this, duodenal fluid was collected from the first and second portions of the duodenum. For the serological assaying of anti-Hpy/oriIgG and IgA, and anti-Hpy/oriIgA in duodenal fluids, the ELISA method was utilized. RESULTS: The concentration of serum IgG showed sensitivity of 64.0%, specificity of 83.7%, positive predictive value of 82.0%, negative predictive value of 66.6% and accuracy of 73.1% for the diagnosis of H pyloriinfection. For the same purpose, serum IgA showed sensitivity of 72.0%, specificity of 65.9%, positive predictive value of 72.0%, negative predictive value of 67.4% and accuracy of 69.8%. If the serological tests were considered together, i.e. when both were positive or negative, the accuracy was 80.0%, sensitivity was 86.6%, specificity was 74.2%, positive predictive value was 74.2% and negative predictive value was 86.6%. When values obtained in the test for detecting IgA in the duodenal fluid were analyzed, no significant difference (P=0.43) was observed between the values obtained from patients with or without Hpyloriinfection. CONCLUSION: The results of serum IgG and IgA tests for H pylori detection when used simultaneously, are more efficient in accuracy, sensitivity and negative predictive value, than those when used alone. The concentration of IgA antibodies in duodenal fluid is not useful in identifying patients with or without H pylori.

幽门螺杆菌CagA抗体检测的间接ELISA法建立及评价

目的制备高纯度、具有生物活性的CagA蛋白,建立间接ELISA法检测CagA抗体,并对该方法进行评估。方法构建重组表达质粒CagA-PET28a,转化至大肠杆菌BL21(DE3)中诱导表达,利用蛋白纯化液相色谱仪进行镍亲和层析纯化后再进行离子交换层析纯化。利用验证后的蛋白建立了间接ELISA法,通过与市售试剂盒进行比对,对间接ELISA法进行评价。结果成功表达并纯化出了高纯度的CagA蛋白,分子量为130 kD,纯度为99.87%。利用该抗原建立了间接ELISA法,并对204例待测血清进行检测。与Mikrogen公司血清学检测试剂盒的结果比较,阳性符合率为86.5%,阴性符合率为91%,总符合率为88.7%。对伯劳特公司提供的参考品进行检测,表明该间接ELISA法具有较好的重复性且最低检测限检测结果符合预期。结论利用重组CagA蛋白建立的检测患者血清中CagA抗体的方法具有较好的检测性能,且重复性较好,最低检测限与市售试剂盒相当。

商用血清学试剂盒诊断幽门螺杆菌现症感染的应用评估

背景:寻找操作简便的非侵入性检测方法对于幽门螺杆菌(H.pylori)现症感染的诊断非常重要。目的:评估应用胶体金免疫层析技术的商用血清学试剂盒在诊断H.pylori现症感染中的应用价值。方法:167名健康志愿者纳入本研究。采集血标本,以Assure^(?)H.pylori快速检测试剂盒行免疫层析。同时行^(13)C-尿素呼气试验(^(13)C-UBT)和内镜活检组织病理学检查,两项检查均阳性定义为H.pylori感染,两项检查均阴性定义为H.pylori阴性。结果:根据诊断标准,147例纳入统计分析者的H.pylori感染率为65.3%,Assure^(?)H.pylori快速检测的现症感染阳性率为68.7%,其诊断敏感性、特异性、阳性预测值、阴性预测值和准确性分别为96.9%、84.3%、92.1%、93.5%和92.5%。结论:Assure^(?) H.pylori快速检测是一种简单、快速、可靠的非侵入性H.pylori现症感染诊断方法,在流行病学调查中有很好的应用前景。

血清学检测在快速尿素酶试验假阴性出血性溃疡患者中的诊断价值

目的观察快速尿素酶试验（RUT）在出血性消化性溃疡患者中敏感性,探讨血清学检测（免疫印迹法,IBT）能否提高RUT假阴性消化性溃疡并出血患者H.pylori感染检出率。方法采用RUT、组织学（W-S染色法）和IBT三种方法检测2009年1月~8月经胃镜确诊的39例出血性消化性溃疡（出血组）住院患者H.pylori感染情况与33例非出血性消化性溃疡（非出血组）门诊患者进行对照研究。以最少两种诊断方法阳性为H.pylori感染诊断参照标准。结果RUT在出血组H.pylori阳性率43.6%低于非出血组81.8%（P=0.001）,差异有统计学意义。出血组IBT阳性率87.2%明显高于RUT阳性率43.6%（P〈0.001）;也高于W-S染色法阳性率64.1%,差异有统计学意义（P=0.012）。出血组RUT灵敏度65.4%显著低于非出血组96.3%（P=0.005）。出血组RUT阴性患者22例,依参照标准IBT可检出RUT假阴性患者9例,在出血性消化性溃疡RUT阴性患者中H.pylori检出率为40.9%。结论RUT在出血性消化性溃疡患者中灵敏度降低,假阴性患者增加。血清学（IBT）可作为H.pylori感染出血性消化性溃疡RUT阴性患者的补救措施或可选方法之一,有助于提高消化性溃疡并出血时H.pylori感染检出率。

幽门螺杆菌感染三种诊断方法的分析评价

目的 :对幽门螺杆菌 (Hp)感染的三种临床诊断方法——病理组织学、血清学、快速尿素酶试验的敏感性、特异性、有效性、阳性预测值及阴性预测值进行分析评价。方法 :对 186例因上腹不适的病人进行胃镜检查并取胃粘膜组织作快速尿素酶试验及病理组织学检查 Hp,同时抽血做血清抗幽门螺杆菌抗体测定 ,其中二项阳性认为有 Hp感染。结果 :病理组织学、血清学、快速尿素酶试验的敏感性分别为 81.8% ,93.5 % ,84.6 % ;特异性分别为 96 .7% ,83.7% ,81.4% ;有效性分别为 85 .5 % ,90 .9% ,84.9% ;阳性预测值分别为 98.2 % ,94.1% ,92 .9% ;阴性预测值分别为 6 5 .3% ,82 .0 % ,6 7.8%。结论 :三种方法均有较高的敏感性、特异性及有效性 ,尤以病理组织学特异性最高。对于 Hp的诊断参考 ,在一般临床工作中可任选其中一种。

规范幽门螺杆菌感染诊断,提高诊断准确性

根除幽门螺杆菌(Hp)是防治慢性胃炎、消化性溃疡和胃癌的重要措施,我国Hp感染处理共识已将“证实有Hp感染”作为根除指征。世界胃肠病学组织指南指出,“治疗所有Hp阳性者,如无意治疗就不要检测”。准确诊断Hp感染是规范化根除治疗的前提。Hp感染的诊断方法多样,各有其优缺点,不同方法适用于不同疾病和患者,每种方法对试剂、设备、检测者和患者都有严格的要求。因此,提高临床医师和检测者对规范诊断的认知,对提高Hp感染诊断的准确性至关重要。

幽门螺杆菌与妊娠剧吐的相关性影响分析

目的分析影响妊娠剧吐患者幽门螺旋杆菌的重要因素,并提示幽门螺旋杆菌感染与妊娠剧吐间的关系。方法回顾分析我院于2019年2月至2020年3月收治的妊娠剧吐且合并油门螺旋杆菌感染的患者76例为观察组,并选取同期我院收治的妊娠剧吐患者76例为对照组。选定患者后,搜集患者的基本情况,对患者资料进行分析。首先统计患者的基础信息情况,其后统计患者生理检测情况。最后,对统计信息进行Logistic回归分析,明确肋骨骨折后引发血胸的独立危险因素。结果观察组年龄因素、身高因素、体重因素、孕周因素、B超胎儿质量及心理问题(焦虑、抑郁)评分情况无对照组相比无显著差异,无统计学意义(P>0.05);观察组HCG显著升高比率显著高于对照组;观察组消化不良比率显著高于对照组;观察组糖皮质激素过高比率、盐皮质激素过高比率及性腺激素过高比率显著高于对照组;观察组机体酸碱度偏酸比率显著高于对照组,差异均具有统计学意义(P=0.001);HCG显著升高、消化不良、糖皮质激素过高、盐皮质激素过高、性腺激素过高及机体酸碱度等因素均是影响素妊娠剧吐合并幽门螺旋杆菌的重要因素。差异均具有统计学意义(P=0.001)。妊娠剧吐与发生幽门螺旋杆菌感染之间存在密切关系。结论妊娠剧吐与发生幽门螺旋杆菌感染之间存在密切关系,但关系并对绝对,当孕妇发生HCG显著升高、消化不良、糖皮质激素过高、盐皮质激素过高、性腺激素过高及机体酸碱度偏酸等情况时,则容易发生幽门螺旋杆菌感染。

不同幽门螺杆菌感染检测方法的评价

目的 :评价适合临床使用的检测幽门螺杆菌 (Helicobacterpylori,Hp)的方法。方法 :332例入选患者进行快速尿素酶 ,病理组织学 ,现症感染血清学抗体快速检测法 (CIM -GLD’sHpRT) ,3项中 2项阳性认为有Hp感染。结果 :CIM -GLD’sHpRT ,病理组织学 ,快速尿素酶法敏感性分别为 95 .77%、91.0 1%、81.4 8% ;特异性分别为 95 .10 %、96 .5 0 %、93.0 1% ;阳性预测值分别为 96 .2 2 %、97.18%、93.90 % ;阴性预测值分别为 94 .37%、89.0 3%、79.17%。结论 :CIM -GLD’sHpRT具有较高的准确诊断HP感染的价值。

幽门螺杆菌感染诊断的研究进展

内镜检查技术正在不断完善,除了窄带成像技术外,蓝激光成像、联动成像以及结合人工智能的卷积神经网络等技术,都可运用于观察幽门螺杆菌(Hp)感染。当慢性活动性胃炎患者经标准染色法未检测到Hp感染,或存在罕见、特殊细菌感染时,临床建议加做免疫组化。以实时聚合酶链式反应(PCR)为基础的分子方法在检测Hp感染和耐药Hp中均显示出很高的准确性;最可靠的非侵入性方法仍然是尿素呼气试验;大便抗原检测敏感性与尿素呼气试验可相媲美,并且可能是后者的良好替代方法。本文将Hp感染诊断的相关方法分为侵入性和非侵入性两大类,就Hp感染诊断的相关方法作一综述。

应用血清学及^(13)C-尿素呼吸试验对幽门螺杆菌家庭感染状况的研究

目的 研究一级亲属幽门螺杆菌 (helicobacterpylori,HP)感染状况以及其对儿童HP感染的影响。方法 应用血清学及13 C -尿素呼吸试验 (13 C -ureabreathtest,13 C -UBT)测定反复腹痛儿童及父母的HP感染情况。结果 13 C -UBT及血清HP -IgG测定家庭的HP感染率分别为 41 .6 %和 76 .5 % ,显著高于腹痛儿童的HP感染率 2 9.5 %和 5 4.8% (P <0 .0 5和P <0 .0 0 5 )。儿童13 C -UBT及血清学阳性时 ,其家庭HP感染率分别为83.7%和 85 .7% ,显著高于其儿童阴性时的家庭HP感染率 2 3.9%和 6 5 .3% ;家庭13 C -UBT及血清学阳性时 ,其儿童HP感染率分别为 5 9.4%和 6 1 .4% ,也显著高于其家庭阴性的儿童HP感染率 8.3%和 33.3% ,P <0 .0 0 5。血清学检查的敏感性为 94.2 % ,特异性 36 .1 %。结论 一级亲属有HP带菌的家庭中儿童HP症状性感染率高 ,HP感染有明显的家庭聚集性。血清HP -IgG检测敏感性好 ,特异性差 ,13 C -UBT比血清学检测更能反应HP家庭实时感染状况。

安徽淮南地区160例幽门螺杆菌培养结果分析及药敏试验指导临床治疗的效果研究

目的 了解安徽淮南地区幽门螺杆菌(Helicobacter Pylori, HP)培养及耐药状况,为临床诊治HP感染提供实验室依据。方法 选择2018年7月—2021年12月于淮南东方医院集团总医院就诊的160例消化性溃疡患者为研究对象,取胃窦黏膜病灶组织进行HP培养及药物敏感性(药敏)试验,并分析结果。按照随机数字表法,将HP培养阳性患者分为对照组和观察组。其中对照组采用常规/经验四联疗法治疗方案(阿莫西林胶囊、克拉霉素、奥美拉唑、枸橼酸铋钾胶囊),观察组在对照组基础上根据药敏试验选择耐药率较低的抗菌药物四联疗法方案(甲硝唑、呋喃唑酮、奥美拉唑、枸橼酸铋钾胶囊)指导临床治疗,2组患者均完成2周药物治疗,比较2组血清胃泌素-17(gastrin-17, G-17)、胃蛋白酶原I(pepsinogen I,PGI)、胃蛋白酶原II(pepsinogenII,PGII)水平,HP根除率及不良反应情况。结果 160例患者完成HP培养,其中56例患者阳性,阳性率为35.00%(56/160)。药敏试验结果表明,耐药率排在前2位的抗菌药物分别为左氧氟沙星(96.43%,54/56)和四环素(92.86%,52/56)。对照组和观察组治疗前血清G-17、PGI及PGII水平比较差异均无统计意义(P均> 0.05),治疗2周后,血清G-17、PGI及PGII水平均低于治疗前(P均<0.05)。观察组治疗2周后血清G-17、PGI及PGII水平下降幅度均高于对照组(P均<0.05)。观察组疗程完毕后,随访4、8、12个月时HP根除率均高于对照组(P均<0.05)。2组用药期间不良反应发生率比较差异无统计意义(P> 0.05)。结论 安徽淮南地区HP感染后培养阳性率较高,且对左氧氟沙星、四环素等抗菌药物耐药率较高。HP培养阳性的消化性溃疡患者应根据药敏试验结果合理选择应用抗菌药物,不仅有助于改善血清学水平,提高HP根除率,而且用药安全性较高。

幽门螺杆菌分型与妊娠剧吐相关性研究

目的探讨幽门螺杆菌分型与妊娠剧吐的相关性。方法选择60例妊娠剧吐孕妇为研究组,65例正常孕妇为对照组,用免疫印迹法进行幽门螺杆菌分型检测,观察两组孕妇不同类型幽门螺杆菌感染构成比的差异。结果妊娠剧吐孕妇Ⅰ型(毒力型)幽门螺杆菌感染的比率显著高于对照组(2χ=12.67,P<0.01)。结论Ⅰ型幽门螺杆菌感染与妊娠剧吐有显著的相关性。

血清H.pylori抗体抗Hp细胞毒素相关基因A及13C-尿素呼气实验结果与湖州地区孕妇妊娠剧吐的相关性分析

目的分析血清H.pylori抗体、抗Hp细胞毒素相关基因A(CagA)及13C-尿素呼气实验结果与湖州地区孕妇妊娠剧吐的相关性。方法选取2019年7月—2020年6月于湖州市妇幼保健院就诊的妊娠剧吐(观察组)与无妊娠呕吐(对照组)的孕妇各100例进行研究,并将观察组按病情严重程度分为重度组与轻度组,入组后检测血清H.pylori抗体、抗Hp细胞毒素相关基因A(CagA)及进行13C-尿素呼气实验,比较各组检测结果、孕前及孕早期体质量、孕妇并发症及围生儿不良妊娠结局。结果观察组孕妇Hp、CagA及13C-尿素呼气阳性率均高于对照组,差异均有统计学意义(χ^(2)=26.299,44.907,28.926,均P<0.05)。将观察组孕妇按病情严重程度分组,重度组孕妇Hp及13C-尿素呼气阳性率均高于轻度组(χ^(2)=4.005,5.654,均P<0.05)。CagA阳性率组间比较差异无统计学意义(χ^(2)=3.287,P>0.05)。孕早期观察组体质量较孕前减轻,对照组较孕前增重,差异有统计学意义(t=0.279,P<0.05)。孕早期观察组体质量较对照组轻,差异有统计学意义(t=3.357,P<0.05)。两组孕妇并发症发生率比较差异无统计学意义(P<0.05)。观察组小于胎龄儿、低出生体质量发生率及不良妊娠结局总发生率高于对照组(P<0.05)。结论Hp感染与妊娠剧吐密切相关,妊娠剧吐可导致孕妇体质量减轻、不良妊娠结局发生率增高。

血清胃蛋白酶原、幽门螺杆菌联合内镜下木村-竹本分类在早期胃癌诊断中的价值

目的探讨血清胃蛋白酶原(PG)、幽门螺杆菌(Hp)联合内镜下木村-竹本分类在早期胃癌诊断中的应用价值。方法选取2022年1-6月在湖州市第一人民医院消化内科就诊的胃癌患者60例(胃癌组),另选取同期该院收治的胃良性病变患者60例(胃良性病变组)和胃癌前病变患者60例(胃癌前病变组),均进行血清PG检测、Hp检测及内镜下木村-竹本分类,评估其在早期胃癌诊断中的应用价值。结果与胃良性病变组和胃癌前病变组患者比较,胃癌组患者血清PGⅠ/PGⅡ比值(PGR)均明显降低,而PGⅡ水平和Hp感染率[71.67%(43/60)]均明显升高(F=108.14、71.75、38.43,χ^(2)=6.89,均P<0.05)。与胃良性病变组和胃癌前病变组比较,胃癌组的木村-竹本分型明显加重(H=38.91,P<0.05)。胃癌组血清PGⅠ和PGR随病理分期的增高而降低,而PGⅡ水平随病理分期的增高而上升(F=65.79、5.66、53.32,均P<0.01);但各期患者的Hp感染率差异无统计学意义(P>0.05)。胃癌组各期患者的木村-竹本分型差异均无统计学意义(均P>0.05)。受试者工作特征曲线分析显示,PGⅠ、PGⅡ、PGR诊断胃癌的曲线下面积分别为0.865、0.664、0.881。结论血清PG、Hp联合内镜下木村-竹本分类能够提高早期胃癌的诊断率,木村-竹本分类法有助于胃癌患者在内镜筛查中进行风险分层,并与血清PG水平存在一致性,具有较高的应用价值。

儿童幽门螺杆菌感染与贫血、铁缺乏症的关系

目的了解武汉地区2～6岁儿童幽门螺杆菌(Hp)感染与贫血、铁缺乏症的流行病学特征,探讨Hp感染与儿童贫血、铁缺乏症之间的关系。方法随机选取95例在本院体检的儿童作为研究对象,采用2种方法对所有儿童进行Hp感染检测。应用ELISA方法测定血清IgG水平,以评估Hp既往感染;采用14C尿素呼气试验(14C-UBT)对所有儿童进行检测,以评估Hp目前活动型感染。清晨采集空腹静脉血3mL,分离血清检测Hp-IgG、血清铁蛋白,采用血红蛋白计检测其Hb水平,通过测定血清CRP水平评估近期炎性反应及感染情况。记录每名儿童年龄、性别、近几个月内抗生素使用情况。将研究对象分为Hp感染组和非Hp感染组。采用Logistic回归方法分析Hp感染对儿童贫血、铁缺乏症的影响效应,并对Hp感染的2种检验方法行Kappa一致性分析。结果95名儿童中,18.9%有贫血,36.8%存在铁缺乏症,40.0%Hp-IgG阳性,74.7%UBT阳性。在对人口统计学因素、近期感染、近期抗生素使用情况进行调整后分析显示:Hp-IgG阳性是儿童贫血、铁缺乏症的重要危险因素。结论使用不同方法检测Hp可反映感染的不同阶段。UBT阳性反映Hp感染的早期阶段;血清Hp抗体阳性则提示Hp感染处于后期阶段,并与儿童贫血、铁缺乏症密切相关。