Objective:To measure serum copper and protein thiols along with lipid profile in hyperlipidemic patients and to establish the relationship between serum copper,protein thiols and lipid profile parameters.Methods: The ...Objective:To measure serum copper and protein thiols along with lipid profile in hyperlipidemic patients and to establish the relationship between serum copper,protein thiols and lipid profile parameters.Methods: The study group consisted of 26 newly diagnosed hyperlipidemic patients not associated with any other systemic diseases and 26 age and sex matched healthy controls.Fasting lipid profile was done by enzymatic method using automated analyzer.Serum copper and protein thiols were measured spectrophotometrically.Results: Serum copper and protein-SH levels were significantly decreased in cases(P<0.01,P<0.01) compared to controls.Serum copper correlated positively with protein thiols(r=0.800,P<0.01) and negatively with LDL cholesterol(r=-0.351 P<0.05).Conclusion: Serum copper and protein thiols were decreased in hyperlipidemia and they correlated positively indicating protective role of copper in preventing homocysteinylation of protein and hence in preventing atherosclerosis.展开更多
The 18 crude drugs in Bofutsushosan (BOF: Pulvis ledebouriellae compositae: 防風通聖散) are separated into 6 groups such as diaphoretic, cathartic, antidote, antipyretic, neutralizer and diuretic groups. The effects o...The 18 crude drugs in Bofutsushosan (BOF: Pulvis ledebouriellae compositae: 防風通聖散) are separated into 6 groups such as diaphoretic, cathartic, antidote, antipyretic, neutralizer and diuretic groups. The effects of single administered BOF and composed crude drugs in 6 groups were investigated on the levels of diabetic parameters (serum glucose, insulin, triglyceride and cholesterol) in streptozotocin-induced diabetic mice. The anti-hyperglycemic action of BOF was depended on Ephedrae Herba, Saposhnikoviae Radix and Schizonepetae Spica in diaphoretic group, Forsythiae Fructus, Saposhnikoviae Radix, Schizonepetae Spica and Cnidii Rhizoma in antidote group, Scutellariae Radix, Gardeniae Fructus and Gypsum Fibrosum in antipyretic group and Paeoniae Radix in neutralizer group. In these crude drugs, Ephedrae Herba, Saposhnikoviae Radix, Schizonepetae Spica, Forsythiae Fructus, Scutellariae Radix, Gypsum Fibrosum and Paeoniae Radix increased serum insulin level, but Cnidii Rhizoma and Gardeniae Fructus did not affect serum insulin level. From these results, it suggested that anti-hyperglycemic action of BOF was through insulin-dependent and insulin independent manners. The lowering effect of BOF on serum triglyceride level was dependent on actions of Platycodi Radix in antidote and diuretic groups and Gardeniae Fructus in antipyretic group. The lowering effect of Gardeniae Fructus was parallel with its anti-hyperglycemic action. The lowering effect of BOF on high serum triglyceride level also included both direct action and indirect action. The reducing effect of BOF on serum cholesterol level was observed together with the actions of Ephedrae Herba and Zingiberis Rhizoma in diaphoretic group, Schizonepetae Spica in diaphoretic and antidote groups and Paeoniae Radix in neutralizer group. The lowering effects of Ephedrae Herba, Schizonepetae Spica and Paeoniae Radix on serum cholesterol level were parallel with their anti-hyperglycemic actions. Zingiberis Rhizoma in diaphoretic group might be direct reducing effect on serum cholesterol level but no serum glucose level. The Ephedrae Herba in diaphoretic group, Schizonepetae Spica in diaphoretic and antidote groups and Paeoniae Radix in neutralizer group might have reduced serum cholesterol level by reducing blood glucose level. From these results, composed crude drugs in 6 groups show various mechanisms in the action of BOF.展开更多
To study the effet of scallop extract from the experimental rats on blood lipid and lipoperoxidation,40 rats(Wislin, The weight of body is about 200 (±20g) had been fed with the high fat diet for 30 days to abtai...To study the effet of scallop extract from the experimental rats on blood lipid and lipoperoxidation,40 rats(Wislin, The weight of body is about 200 (±20g) had been fed with the high fat diet for 30 days to abtain hyperlipidemic rat model.Then the 40 rats were randomly divided into four groups (10 rats /group): Hyperlipidemia group and scallop extract group. Hyperlipidemia group was continuously fed with the high fat diet. Scallop extract group was given scallop extract by gastrointestinal injection at a daily dose when raised with the high fat diet. Moreover the control group (10 rats /group) raised with the basal diet was established. The test had been conducted for 30 days. The blood of rat tail was taken out to determine the levels of TC, TG, LDL C, HDL C, GSH Px, SOD and LPO. The results showed that scallop extract could markedly decrease the contents of TC, TG, LDL C in the serum of the experimental hyperlipidemic rats(P<0.01), and significantly increase the levels of serum HDL C (P<0.01). Levels of blood LPO in groups of scallop extract were much lower than that of in hyperlipidemia group. Meanwhile, the GSH Px and SOD activities of blood in groups of scallop extract were much higher than those of in Hyperlipidemia group. The results revealed that scallop extract had a strong antihyperlipidemic activity and had the effects of enhancing the antioxidation and reducing the lipid peroxidation.展开更多
文摘Objective:To measure serum copper and protein thiols along with lipid profile in hyperlipidemic patients and to establish the relationship between serum copper,protein thiols and lipid profile parameters.Methods: The study group consisted of 26 newly diagnosed hyperlipidemic patients not associated with any other systemic diseases and 26 age and sex matched healthy controls.Fasting lipid profile was done by enzymatic method using automated analyzer.Serum copper and protein thiols were measured spectrophotometrically.Results: Serum copper and protein-SH levels were significantly decreased in cases(P<0.01,P<0.01) compared to controls.Serum copper correlated positively with protein thiols(r=0.800,P<0.01) and negatively with LDL cholesterol(r=-0.351 P<0.05).Conclusion: Serum copper and protein thiols were decreased in hyperlipidemia and they correlated positively indicating protective role of copper in preventing homocysteinylation of protein and hence in preventing atherosclerosis.
文摘The 18 crude drugs in Bofutsushosan (BOF: Pulvis ledebouriellae compositae: 防風通聖散) are separated into 6 groups such as diaphoretic, cathartic, antidote, antipyretic, neutralizer and diuretic groups. The effects of single administered BOF and composed crude drugs in 6 groups were investigated on the levels of diabetic parameters (serum glucose, insulin, triglyceride and cholesterol) in streptozotocin-induced diabetic mice. The anti-hyperglycemic action of BOF was depended on Ephedrae Herba, Saposhnikoviae Radix and Schizonepetae Spica in diaphoretic group, Forsythiae Fructus, Saposhnikoviae Radix, Schizonepetae Spica and Cnidii Rhizoma in antidote group, Scutellariae Radix, Gardeniae Fructus and Gypsum Fibrosum in antipyretic group and Paeoniae Radix in neutralizer group. In these crude drugs, Ephedrae Herba, Saposhnikoviae Radix, Schizonepetae Spica, Forsythiae Fructus, Scutellariae Radix, Gypsum Fibrosum and Paeoniae Radix increased serum insulin level, but Cnidii Rhizoma and Gardeniae Fructus did not affect serum insulin level. From these results, it suggested that anti-hyperglycemic action of BOF was through insulin-dependent and insulin independent manners. The lowering effect of BOF on serum triglyceride level was dependent on actions of Platycodi Radix in antidote and diuretic groups and Gardeniae Fructus in antipyretic group. The lowering effect of Gardeniae Fructus was parallel with its anti-hyperglycemic action. The lowering effect of BOF on high serum triglyceride level also included both direct action and indirect action. The reducing effect of BOF on serum cholesterol level was observed together with the actions of Ephedrae Herba and Zingiberis Rhizoma in diaphoretic group, Schizonepetae Spica in diaphoretic and antidote groups and Paeoniae Radix in neutralizer group. The lowering effects of Ephedrae Herba, Schizonepetae Spica and Paeoniae Radix on serum cholesterol level were parallel with their anti-hyperglycemic actions. Zingiberis Rhizoma in diaphoretic group might be direct reducing effect on serum cholesterol level but no serum glucose level. The Ephedrae Herba in diaphoretic group, Schizonepetae Spica in diaphoretic and antidote groups and Paeoniae Radix in neutralizer group might have reduced serum cholesterol level by reducing blood glucose level. From these results, composed crude drugs in 6 groups show various mechanisms in the action of BOF.
文摘To study the effet of scallop extract from the experimental rats on blood lipid and lipoperoxidation,40 rats(Wislin, The weight of body is about 200 (±20g) had been fed with the high fat diet for 30 days to abtain hyperlipidemic rat model.Then the 40 rats were randomly divided into four groups (10 rats /group): Hyperlipidemia group and scallop extract group. Hyperlipidemia group was continuously fed with the high fat diet. Scallop extract group was given scallop extract by gastrointestinal injection at a daily dose when raised with the high fat diet. Moreover the control group (10 rats /group) raised with the basal diet was established. The test had been conducted for 30 days. The blood of rat tail was taken out to determine the levels of TC, TG, LDL C, HDL C, GSH Px, SOD and LPO. The results showed that scallop extract could markedly decrease the contents of TC, TG, LDL C in the serum of the experimental hyperlipidemic rats(P<0.01), and significantly increase the levels of serum HDL C (P<0.01). Levels of blood LPO in groups of scallop extract were much lower than that of in hyperlipidemia group. Meanwhile, the GSH Px and SOD activities of blood in groups of scallop extract were much higher than those of in Hyperlipidemia group. The results revealed that scallop extract had a strong antihyperlipidemic activity and had the effects of enhancing the antioxidation and reducing the lipid peroxidation.
