Objective: To build a cell model of LPS-induced hyper- and hypo-responsiveness in macrophage cells . Methods: Macrophage cell line RAW264. 7 was pre-cultured with or without 10 ng/ml LPS for 18 h, then challenged with...Objective: To build a cell model of LPS-induced hyper- and hypo-responsiveness in macrophage cells . Methods: Macrophage cell line RAW264. 7 was pre-cultured with or without 10 ng/ml LPS for 18 h, then challenged with lipopolysaccharide(LPS) , or MDP, Zymosan, PAF, FMLP, PMA for 24 h. The levels of TNF-α , IL-1 , IL-6, IL-10 , NO and O2-, were measured. Results: LI'S pretreatment markedly inhibited TNF-a NO and IL-6 production, but increased IL-1, IL-10 and O2- release to LPS challenge. LPS pretreatment also altered macrophage responsiveness to the other stimuli. Conclusion: LPS can induce hyper- and hypo-responsiveness simultaneously in the macrophage cell lines. Changes in macrophage responsiveness depend on stimuli and effectors which are measured.展开更多
基金Supported by National Natural Science Foundation of China (No.39730210)
文摘Objective: To build a cell model of LPS-induced hyper- and hypo-responsiveness in macrophage cells . Methods: Macrophage cell line RAW264. 7 was pre-cultured with or without 10 ng/ml LPS for 18 h, then challenged with lipopolysaccharide(LPS) , or MDP, Zymosan, PAF, FMLP, PMA for 24 h. The levels of TNF-α , IL-1 , IL-6, IL-10 , NO and O2-, were measured. Results: LI'S pretreatment markedly inhibited TNF-a NO and IL-6 production, but increased IL-1, IL-10 and O2- release to LPS challenge. LPS pretreatment also altered macrophage responsiveness to the other stimuli. Conclusion: LPS can induce hyper- and hypo-responsiveness simultaneously in the macrophage cell lines. Changes in macrophage responsiveness depend on stimuli and effectors which are measured.
