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胃酸减少导致不同鼠龄大鼠胃内细菌过度生长及逆向定植 被引量:1
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作者 朱鸣 吴本俨 +1 位作者 宫媛 王昌正 《胃肠病学和肝病学杂志》 CAS 2010年第2期134-137,共4页
目的研究大鼠胃内pH值的变化与胃及肺部细菌数量变化的关系,观察是否存在大鼠胃内细菌到肺部的逆向定植。方法3月龄Wister大鼠20只,22月龄Wister大鼠12只,各分为2组,老龄、非老龄奥美拉唑组,老龄、非老龄对照组。奥美拉唑组以奥美拉唑灌... 目的研究大鼠胃内pH值的变化与胃及肺部细菌数量变化的关系,观察是否存在大鼠胃内细菌到肺部的逆向定植。方法3月龄Wister大鼠20只,22月龄Wister大鼠12只,各分为2组,老龄、非老龄奥美拉唑组,老龄、非老龄对照组。奥美拉唑组以奥美拉唑灌胃(30mg.kg-1.d-1),对照组以等量的生理盐水灌胃。第4d开始,连续10d给大鼠灌饲含质粒pGEX-4T-1-EGFP的大肠杆菌DH5α(10mL/kg,1次/d)。第14d测胃内pH值,取胃及肺组织做细菌培养。应用荧光显微镜和质粒酶切电泳鉴定细菌的来源。结果荧光显微镜下观察大鼠的胃及肺部分离的大肠杆菌,可见明确的绿色荧光。胃和肺分离出的细菌经质粒酶切电泳鉴定,可见目的条带。非老龄奥美拉唑组大鼠胃内pH值和胃内大肠杆菌数均高于非老龄对照组(P<0.01),肺部大肠杆菌数无统计学差异(P>0.05)。老龄奥美拉唑组大鼠胃内pH值(P<0.01)及胃(P<0.01)和肺部大肠杆菌数(P<0.05)均高于老龄对照组。老龄奥美拉唑组大鼠胃内pH值(P>0.05)及胃内大肠杆菌数(P>0.05)与非老龄奥美拉唑组相比无统计学差异,但肺内大肠杆菌数(P<0.01)明显高于非老龄奥美拉唑组。结论随胃内pH值升高,大鼠胃内大肠杆菌数量增加,推测大鼠胃内含pGEX-4T-1-EGFP的大肠杆菌可以转移到肺部。随胃内pH值升高,老龄大鼠肺部的大肠杆菌数增高,且高于非老龄大鼠。 展开更多
关键词 胃酸减少 细菌过度生长 细菌逆向定植 衰老
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Hepatic abscess associated with Salmonella serotype B in a chronic alcoholic patient 被引量:1
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作者 Sidhartha S Tulachan Charles E Wilkins +2 位作者 Anthony F Cutrona David Gemmel Thomas P Marnejon 《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS 2013年第4期440-442,共3页
BACKGROUND: Hepatic abscess secondary to Salmonella is extremely rare in the United States and other Western countries. METHODS: A 43-year-old Caucasian man, with a history of chronic alcohol abuse, was admitted to th... BACKGROUND: Hepatic abscess secondary to Salmonella is extremely rare in the United States and other Western countries. METHODS: A 43-year-old Caucasian man, with a history of chronic alcohol abuse, was admitted to the hospital for intermittent abdominal pain, fever and diarrhea. Clinical radiological and laboratory results were analyzed. Medical literature in PubMed pertaining to similar cases was reviewed. RESULTS: Stool culture was positive for Salmonella serotype B and a CT scan of the abdomen with contrast was consistent with a solitary hepatic abscess. Appropriate intravenous antibiotics followed by oral maintenance therapy for six weeks resulted in a complete clinical recovery and radiographic resolution. CONCLUSIONS: The cause of Salmonella hepatic abscess in our patient was most likely associated with decreased mucosal resistance to the bacteria, seeding of infection via transient portal bacteremia and loss of host immunity. Our case highlights the fact that appropriate antibiotic alone is sufficient in the management of a solitary hepatic abscess less than 3-5 cm. 展开更多
关键词 hepatic abscess SALMONELLA ALCOHOLIC hypochlorhydria ANTIBIOTICS
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