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国槐DNA导入花生栽培品种选育抗叶斑病新种质的鉴定 被引量:12
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作者 刘风珍 万勇善 薛其勤 《华北农学报》 CSCD 北大核心 2010年第6期113-117,共5页
为了培育抗花生叶斑病的新种质,利用花粉管通道技术将国槐DNA导入花生栽培品种79266,在变异后代筛选抗叶斑病材料,对选出的5个种质系进行叶斑病抗性和农艺性状的田间鉴定,并进行抗病种质系与受体DNA差异的SSR多态性分析。获得的主要结... 为了培育抗花生叶斑病的新种质,利用花粉管通道技术将国槐DNA导入花生栽培品种79266,在变异后代筛选抗叶斑病材料,对选出的5个种质系进行叶斑病抗性和农艺性状的田间鉴定,并进行抗病种质系与受体DNA差异的SSR多态性分析。获得的主要结果如下:受体品种79266感染叶斑病(包含花生褐斑病、黑斑病和网斑病)病程曲线的线下面积(AUDPC值)为205.3,5个抗病种质系的AUDPC值极显著低于受体品种,其中05D1128的AUDPC值最小,为112.0,感病最轻;收获前7 d调查花生褐斑病和网斑病的发病程度,05D1148对花生褐斑病的抗性最强,其病级和病情指数均极显著低于受体品种,抗性比受体品种提高17.56%。05D1128对网斑病的抗性最强,其病级和病情指数均极显著低于受体品种,抗性比受体品种提高31.83%;叶斑病抗性最强的05D1128与受体品种相比,百果重和百仁重明显提高,但荚果产量和籽仁产量极显著降低。褐斑病抗性最强的05D1148与受体品种相比,二者所考察农艺性状间无显著差异;采用40对SSR引物进行PCR扩增,在05D106、05D1128、05D1144、05D1148、05D1172 5个新种质系与受体间检测到的多态性位点数分别是9,8,5,6和7。因此,利用国槐DNA导入花生栽培品种提高其对叶斑病的抗性的育种方法是有效的,所选育种质系中05D1148对叶斑病的抗性和农艺性状的综合表现最好,可以作为新的花生抗叶斑病种质材料加以利用。 展开更多
关键词 花生(Arachis hypogea L) 叶斑病 抗性鉴定 DNA导入技术
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极端嗜热菌Thermotoga hypogea细胞抽提液中醇脱氢酶和氢化酶的活性检测 被引量:1
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作者 王颖 吕巡贤 K.Ma 《新疆农业大学学报》 CAS 2003年第3期75-78,共4页
 极端嗜热菌Thermotogahypogea(T.h.)是一株来自中非油田井下的,能够生长在90℃高温下的短杆状厌氧嗜热菌,经研究发现,在T.h.菌体细胞抽提液中存在一种特殊的NADP依赖性醇脱氢酶(ADH)。该酶在T.h.菌的醇醛代谢途径中起着关键性的催化作...  极端嗜热菌Thermotogahypogea(T.h.)是一株来自中非油田井下的,能够生长在90℃高温下的短杆状厌氧嗜热菌,经研究发现,在T.h.菌体细胞抽提液中存在一种特殊的NADP依赖性醇脱氢酶(ADH)。该酶在T.h.菌的醇醛代谢途径中起着关键性的催化作用,可催化多种醇醛底物的脱氢反应。同时,当以二氯联卞吡啶为作用底物时,T.h.菌的细胞抽提物显示很高的氢化活性(Km=0.29mmol·L-1,Vmax=41.69)。 展开更多
关键词 极端嗜热菌 THERMOTOGA hypogea 醇脱氢酶 氢化酶
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Inoculation of <i>Rhizobiums sp</i>Strains to Improve Soil Fertility: A Peanut Trial in Covèand Ouessè(Benin)
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作者 Alida Adjile Oscar Didagbe +3 位作者 Carrel Ayelo Frechno Boko Pascal Houngnandan Roch Mongbo 《Agricultural Sciences》 2020年第4期448-463,共16页
Thought the increasing demand Arachis hypogaea L. (groundnut), its yields remain low with increasingly using chemical fertilizers. To reduce the costs for chemical fertilizers inquisition and their long-term toxic eff... Thought the increasing demand Arachis hypogaea L. (groundnut), its yields remain low with increasingly using chemical fertilizers. To reduce the costs for chemical fertilizers inquisition and their long-term toxic effects on soils, microbial bio-fertilizers could be an accessible alternative to peanut farms. Thus, the aim of this study was to assess the performance of rhizobia strains on peanut varieties production. The experiments were conducted in two agro-ecological zones of Benin, in a peasant environment peasant-researcher control or under peasant and researcher control. The experimental device used was a complete random block with nine repetitions and two factors namely inoculation (with Rhizobium sp and without Rhizobium sp) and mineral fertilizer (with N15P15K15 and without N15P15K15). The effects of these factors divided into four treatments were evaluated on the plants vegetative, symbiotic and production parameters. In addition, an evaluation of each treatments’ comparative advantages was carried out. The results showed that the association Rhizobium sp and N15P15K15 induced groundnut plants best vegetative and productive parameters. The best comparative advantages in economic terms were also recorded with the same combination (Rhizobium sp + N15P15K15). Considering the technical performance, the recorded treatments effects can be classified as follows: Control Rhizobium sp 15P15K15 Rhizobium sp + N15P15K15. Thus, the association Rhizobium sp + N15P15K15 induced both the best plants vegetative and productive parameters and the best comparative advantage from an economic point of view. The results also showed that the plants’ response to inoculation, the application of manure and their combination was more marked in the bar soil zone (Covè) than in the cotton zone (Ouessè). Considering the negative effects linked to the use of chemical fertilizers, the use of Rhizobium sp could be an interesting path to increase the groundnut production. 展开更多
关键词 ARACHIS hypogeae L. RHIZOBIUM SP Bio Fertilizer Soil Fertility BENIN
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Effect of Gamma Irradiation on Morpho-Agronomic Characteristics of Groundnut (<i>Arachis hypogaea</i>L.) 被引量:3
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作者 L. Tshilenge-Lukanda A. Kalonji-Mbuyi +1 位作者 K. K. C. Nkongolo R. V. Kizungu 《American Journal of Plant Sciences》 2013年第11期2186-2192,共7页
Induced mutation in plant improvement has been used in several crops to generate new sources of genetic variations. A study was conducted to determine the effect of different doses of gamma irradiation on different mo... Induced mutation in plant improvement has been used in several crops to generate new sources of genetic variations. A study was conducted to determine the effect of different doses of gamma irradiation on different morpho-agronomic characteristics. Agronomic traits that were analyzed included: grain yield, number of pods/plant, number of seeds/plant and weight of 100 seeds and numbers of days to 50% flowering. Morphometric characterisation of the descriptive data included plant height, stem diameter, number of leaves/plant, leaflet length, leaflet width and number of ramification/ plant. Groundnut seeds were treated with various doses of gamma rays (100, 200, 400 and 600 Gy). Among the various dose treatments, gamma rays treatment at 100 Gy resulted in a higher increase of grain yield and other morpho-agronomic parameters especially for the JL24 variety. In fact the gamma irradiation at 100 Gy increased significantly grain yield by 14% for JL24, and 4 % for JL12. The number of pods per plant was increased by 2% for JL12 and 37% for JL24. For the number of seeds per plant, there was a significant increase of 8% for JL12, and 62% for JL24 at 100 Gy. A similar trend was observed for the JL24 at 200 Gy dose. Higher doses of gamma rays (400 and 600 Gy) reduced significantly plant growth and grain yield. The usefulness of the mutants identified in a groundnut breeding program is discussed. 展开更多
关键词 Gamma RAY Radiation GROUNDNUT ARACHIS hypogea Grain Yield DR-Congo
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Transferability of Sorghum Genic Microsatellite Markers to Peanut 被引量:3
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作者 Siddanna B. Savadi Bashasab Fakrudin +1 位作者 H. L. Nadaf M. V. C. Gowda 《American Journal of Plant Sciences》 2012年第9期1169-1180,共12页
Currently development of new marker types has shifted from anonymous DNA fragments to gene-based markers. Simple Sequence Repeats (SSRs) are useful DNA markers in plant genetic research including in peanut. However, d... Currently development of new marker types has shifted from anonymous DNA fragments to gene-based markers. Simple Sequence Repeats (SSRs) are useful DNA markers in plant genetic research including in peanut. However, de novo development of SSRs is expensive and time consuming. Gene-based DNA markers are transferable among related species owing to the conserved nature of genes. In this study transferability of sorghum EST-SSR (SbEST-SSR) markers to peanut was prospected. A set of 411 SbEST-SSR primer pairs were used to amplify peanut genomic DNA extracted from cultivated peanut where 39% of them successfully amplified. A comparison of amplification patterns between sorghum and peanut showed similar banding pattern with majority of transferable SbEST-SSRs. Among these transferable SSR markers, 14% have detected polymorphism among 4 resistant and 4 susceptible peanut lines for rust and late leaf spot diseases. These transferable markers will benefit peanut genome research by not only providing additional DNA markers for population genetic analyses, but also allowing comparative mapping to be possible between peanut and sorghum—a possible monocot-dicot comparison. 展开更多
关键词 ARACHIS Hypogea Transferability EST-SSR POLYMORPHISM
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Near-Infrared Analysis of Peanut Seed Skins for Catechins 被引量:2
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作者 Ronald A. Holser 《American Journal of Analytical Chemistry》 2014年第6期378-383,共6页
Peanuts (Arachis hypogea) produce several phenolic compounds such as catechins that may be isolated as co-products from peanut seed testae or skins during peanut processing. Peanut skins are currently underutilized al... Peanuts (Arachis hypogea) produce several phenolic compounds such as catechins that may be isolated as co-products from peanut seed testae or skins during peanut processing. Peanut skins are currently underutilized although they are a potential source of bioactive compounds for use in functional foods and animal feeds. The phenolic compounds in the skins can degrade during processing and lose bioactivity. Extracted peanut skins were analyzed by near-infrared (NIR) spectroscopy to detect phenolic compounds. Spectra were collected from 10000 - 4000 cm﹣1 and evaluated by principal component analysis (PCA) to compare differences in peanut skins before and after alcohol extraction. PCA of derivative spectra showed clear separation into two groups. These results support the development of predictive models to measure catechins in blanched peanut skins. 展开更多
关键词 ANTHOCYANIDINS ARACHIS hypogea NEAR-INFRARED Phenolics SPECTROSCOPY
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Phytochemical Constituents and Determination of Resveratrol from the Roots of <i>Arachis hypogea</i>L. 被引量:1
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作者 Tuyen Pham Nguyen Kim Nga Vo Thi +4 位作者 Phuong Tran Van Phuong Quach Ngo Diem Duong Ngo Thi Thuy Quang Ton That Phung Nguyen Kim Phi 《American Journal of Plant Sciences》 2013年第12期2351-2358,共8页
Two triterpenoid saponins, 6, 7, together with five known compounds were isolated from the roots of Arachis hypogea L. collected in Vietnam. Their structures were established by spectroscopic analysis (1D, 2D NMR and ... Two triterpenoid saponins, 6, 7, together with five known compounds were isolated from the roots of Arachis hypogea L. collected in Vietnam. Their structures were established by spectroscopic analysis (1D, 2D NMR and HRMS) as well as by comparison with those reported in the literature. Furthermore, the high content of resveratrol in the roots opens a new potential resource for pharmaceutical companies to prepare medicines from the wasted peanut roots. 展开更多
关键词 ARACHIS hypogea L. Peanut RESVERATROL TRITERPENOID GLYCOSIDE
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Genetic Diversity of Peanut (Arachis hypogea L.) Cultivars as Revealed by RAPD Markers 被引量:1
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作者 Mohannad G. Al-Saghir Abdel-Salam G. Abdel-Salam 《American Journal of Plant Sciences》 2015年第14期2303-2308,共6页
The objectives of this study were to evaluate the genetic diversity of the peanut accessions using Random Amplified Polymorphic DNA (RAPD) molecular marker and to evaluate RAPD markers to be used in peanut as genetic ... The objectives of this study were to evaluate the genetic diversity of the peanut accessions using Random Amplified Polymorphic DNA (RAPD) molecular marker and to evaluate RAPD markers to be used in peanut as genetic markers and improve such techniques as suitable strategies for peanut germplasm characterization. Twenty peanut accessions were included in this study and were subjected to RAPD molecular markers analysis. Twenty-seven RAPD primers produced 210 amplification products of which 80 (36.4%) were polymorphic. In conclusion, this study reported a successful fingerprinting of peanut accessions using RAPD markers and demonstrated the usefulness of these markers in estimating the extent of genetic variation in peanut germplasm. 展开更多
关键词 RAPD PEANUT ARACHIS hypogea Genetic Diversity
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Low Ca^2+ Content and Ca^2+/Na^+ Ratio in Leaf Tissues Determine Salinity Tolerance in Spanish Type Groundnut (Arachis hypogeal L.)
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作者 Mohammad Akram Hossain Chowdhury Mohammad Shahidur Rashid Bhuyia +1 位作者 Mohammad Shah-e-Alam Mohammad Abul Kalam Azad 《Journal of Agricultural Science and Technology(B)》 2018年第2期71-84,共14页
The study was performed with seven groundnut varieties/genotypes and F1s derived from crossing in all possible combinations without reciprocal among the mentioned varieties/genotypes. The objective was to assess wheth... The study was performed with seven groundnut varieties/genotypes and F1s derived from crossing in all possible combinations without reciprocal among the mentioned varieties/genotypes. The objective was to assess whether low Ca2+ content and Ca2+/Na+ ratio of leaf tissue or stem tissue determine salinity tolerance in terms of economic yield (kernel yield) in groundnut. It revealed that the varieties, “Binachinabadam-6”, “Binachinabadam-5” and the F1 G2 × G3 were most tolerant based on kernel yield under 8 dS/m and 10 dS/m salinity stresses. These two tolerant varieties and the F1 also showed lower Ca2+ and Ca2+/Na+ ratios in leaf tissue, which indicated lower Ca2+ and Ca2+/Na+ ratio of leaf tissue determined salinity tolerance in terms of kernel yield in Spanish type groundnut. These findings could be applied in future plant breeding applications for screening salt tolerant Spanish type groundnut genotypes. 展开更多
关键词 Salinity tolerance GROUNDNUT Arachis hypogea Ca2+ content in leaf tissues Ca2+/Na+ ratio in leaf tissue.
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Influence of <i>Pterocarpus erinaceus</i>Poir. on the Growth and Yields of Groundnut (<i>Arachis hypogea</i>L.) in Agroforestry Parks in the Districts of Tenghory and Kataba 1 (Bignona Department, Lower Casamance)
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作者 Abdou Goudiaby Ismaïla Coly +2 位作者 Aliou Badji Mamadou Kane Daouda Ngom 《American Journal of Plant Sciences》 2021年第10期1515-1527,共13页
In Lower Casamance, <i>Pterocarpus erinaceus</i> occupies a very important place in agroforestry parks. It is often grown in association with the most important cash crop of the area, groundnut <i>(A... In Lower Casamance, <i>Pterocarpus erinaceus</i> occupies a very important place in agroforestry parks. It is often grown in association with the most important cash crop of the area, groundnut <i>(Arachis hypogea L.).</i> Thus, the objective of this study is to contribute to a better understanding of the interactions between trees and groundnut cultivation in agroforestry parks in Lower Casamance. To this end, in each of the two districts (Tenghory and Kataba 1) three <i>Pterocarpus erinaceus</i> trees were selected in three separate communes. For each <i>P. erinaceus</i> tree selected and following each of the four cardinal directions, three situations were defined: at R/2, at 1.5 R and at 2 R from the trunk, with R the radius of the crown. Thus, 12 yield squares were installed around each selected tree, for a total of 72 yield squares installed in the two districts. The parameters measured were the height of the peanut plants, the number of pods produced per plant, and the yield in flakes and pods. The results show that geographical orientation significantly influenced plant height (p = 0.004) but not the number of pods/plant (p = 0.6). As for the distance to the trunk of <i>Pterocarpus erinaceus</i>, it induced a significant variation in the number of pods/plant (p = 0.009) but did not induce a significant effect on the other parameters. Yields varied between 1.56 Tons M S /ha and 2.02 Tons M S /ha for the hay, and between 1.03 Tons M S /ha and 1.45 Tons M S/ha for the pods. The results of this study constitute a useful source of information for a better management of the productivity of the agroforestry parks of the department of Bignona. 展开更多
关键词 Arachis hypogea Pterocarpus erinaceus Yield Tenghory and Kataba 1
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不同株距及穴播粒数对花生(Arachis hypogea L.)光合日变化的影响 被引量:5
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作者 朱冰兵 陈晶晶 +2 位作者 徐惠风 金桥 高华援 《分子植物育种》 CAS CSCD 北大核心 2017年第11期4759-4764,共6页
本试验在大田环境中对不同株距及穴播粒数花生的光合生理指标的日变化进行研究。使用LCpro+型便携式光合仪对花生光合生理指标进行测定。结果表明,密度对花生A(净光合速率)和Gs(气孔导度)具有显著影响,密度越大,A(净光合速率)和Gs(气孔... 本试验在大田环境中对不同株距及穴播粒数花生的光合生理指标的日变化进行研究。使用LCpro+型便携式光合仪对花生光合生理指标进行测定。结果表明,密度对花生A(净光合速率)和Gs(气孔导度)具有显著影响,密度越大,A(净光合速率)和Gs(气孔导度)越低。Ci(胞间CO2浓度)与Gs(气孔导度)呈显著正相关。在株距为25 cm,穴播粒数为2的处理下,Ci(胞间二氧化碳浓度)及Gs(气孔导度)皆与A(光合速率)呈现出显著相关性。通过相关分析探讨不同株距及穴播粒数对花生光合生理指标的影响,以及各光合生理指标间的相互关系,为吉林省的花生高产栽培在理论上提供参考。 展开更多
关键词 花生(Arachis hypogea L.) 株距 穴播粒数 光合速率 日变化
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