野黄瓜(Cucum is hystrix,2n=24)为短日照植物,因其与栽培黄瓜(C.sativus,2n=14)花期不遇等杂交障碍的存在而使种间杂交难以顺利进行。对野黄瓜短光照处理40 d以上,使得两者花期相遇,成功地进行了野黄瓜与3种不同基因型栽培黄瓜(“二早...野黄瓜(Cucum is hystrix,2n=24)为短日照植物,因其与栽培黄瓜(C.sativus,2n=14)花期不遇等杂交障碍的存在而使种间杂交难以顺利进行。对野黄瓜短光照处理40 d以上,使得两者花期相遇,成功地进行了野黄瓜与3种不同基因型栽培黄瓜(“二早子”、“长春密刺”和“NC4406”)的正反杂交,从中获得了3种杂交组合的幼胚。采用改良的方法对这3种基因型杂种胚进行胚胎拯救,再生频率达80%以上,其杂种的真实性通过植物学性状观察、体细胞染色体计数和天冬氨酸转氨酶(AAT)同工酶分析得到了证实。研究中还发现不同基因型杂种F1的育性存在明显差异,其中以C.hystrixדNC4406”育性最高,花粉可染率达23.3%,是在二倍体水平上进行育种利用的良好材料。展开更多
Interspecific hybridization and allopolyploidization contribute to the improvement of many important crops. Recently, we successfully developed an amphidiploid from an interspecific cross between cucumber(Cucumis sati...Interspecific hybridization and allopolyploidization contribute to the improvement of many important crops. Recently, we successfully developed an amphidiploid from an interspecific cross between cucumber(Cucumis sativus, 2n = 2x = 14) and its relative C. hystrix(2n = 2x = 24) followed by chemical induction of chromosome doubling. The resulting allotetraploid plant was self-pollinated for three generations. The fertility and seed set of the amphidiploid plants were very low. In this study, we investigated the meiotic chromosome behavior in pollen mother cells with the aid of fluorescence in situ hybridization, aiming to identify the reasons for the low fertility and seed set in the amphidiploid plants. Homologous chromosome pairing appeared normal, but chromosome laggards were common, owing primarily to asynchronous meiosis of chromosomes from the two donor genomes. We suggest that asynchronous meiotic rhythm between the two parental genomes is the main reason for the low fertility and low seed set of the C. hystrix–cucumber amphidiploid plants.展开更多
Objective:To observe the combination effect of doxorubicin and Citrus hystrix(kaffir lime's)peel ethanolic extract(ChEE)on blood serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)activity and c...Objective:To observe the combination effect of doxorubicin and Citrus hystrix(kaffir lime's)peel ethanolic extract(ChEE)on blood serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)activity and cardio-hepato-histopathology of female Sprague Dawley rats.Methods:Doxorubicin and ChEE(5 rats per group)were administered in five groups of 3 rats each for 11 d.Group I:doxorubicin(dox)4.67 mg/kg body weight;Group II:dox+ChEE 500 mg/kg body weight;Group HI:dox+ChEE 1000 mg/kg body weight;Group IV:ChEE 1000 mg/kg body weight;Group V:untreated(control).Results:ChEE repaired cardiohistopathology profile of doxorubicin induced cardiotoxicity and hepatotoxicity rats,but did not repair neither hepatohistopathology profile nor reduce serum activity of ALT and AST.Conclusion:ChEE has potency to be developed as cardioprotector agent in chemotherapy.展开更多
Both the stoichiometric characteristics and leaf functional traits can reflect the adaptability of plants to changes in the external living environment.In particular,for varying aged forest plants,the study of stoichi...Both the stoichiometric characteristics and leaf functional traits can reflect the adaptability of plants to changes in the external living environment.In particular,for varying aged forest plants,the study of stoichiometric characteristics and leaf functional traits can reflect the plant’s life history strategy and its resource investment and allocation methods.In this paper,the 1.5-year-old,5-year-old,15-year-old and 20-year-old Castanopsis hystrix were selected as the research objects to investigate the trends of stoichiometry and leaf functional traits,and their synergistic changes were verified.The results showed that with the increase of age,the stability of C.hystrix stoichiometry gradually increased,and the functional traits also tended to invest in security.However,there was no synergistic change between the two,which might be attributed to the different sensitivity of the C.hystrix leaf to the environment at the life history level of 1.5-year-old to 20-year-old.展开更多
An interspecific hybrid F1 of Cucumis hystrix Chakr. x Cucumis sativus L. （NC4406） was used to establish the developmental sequence and to characterize the male and female gametophytes at cytological level for furth...An interspecific hybrid F1 of Cucumis hystrix Chakr. x Cucumis sativus L. （NC4406） was used to establish the developmental sequence and to characterize the male and female gametophytes at cytological level for further understanding of the phylogenic relationship and the mechanism of fertility or sterility in the interspecific hybrid F1 The development of male and female gametophytes was studied through meiotic analysis and paraffin section observation technique, respectively. Meanwhile, the fertility level was assessed through hybrid F1 backcrossing to cultivated cucumber 4406. Variable chromosome configurations were observed in the pollen mother cells （PMCs） of hybrid F1 at metaphase Ⅰ , e.g., univalents, bivalents, trivalents, quadravalents, etc. At anaphase Ⅰ and Ⅱ, chromosome lagging and bridges were frequently observed as well, which led to the formation of polyads and only a partial number of microspores could develop into fertile pollen grains （about 23.3%）. Observations of the paraffin sections showed numerous degenerated and abnormal embryo sacs during the development of female gametophytes, and only 40% of the female gametophytes could develop into normal eight-nuclear megaspore. On an average, 22.8 and 6.3 seeds per fruit could be obtained from the reciprocal backcross. The interspecific hybrid F1 of C. hystrix x NC4406 was partially fertile; however, the meiotic behaviors of hybrid F1 showed a high level of intergenomic recombination between C. hystrix and C. sativus chromosomes, which indicated that it plays an important role for introgression of useful traits from C. hystrix into C. sativus.展开更多
Nutrient compositions including proximate, fatty acids, amino acids, minerals and vitamins in tissues of the wild spot-fin porcupinefish(Diodon hystrix) were analyzed in detail to assess their nutrient value and to fu...Nutrient compositions including proximate, fatty acids, amino acids, minerals and vitamins in tissues of the wild spot-fin porcupinefish(Diodon hystrix) were analyzed in detail to assess their nutrient value and to further explore the dietary requirements of this species farmed. Diodon hystrix individuals with similar body weight(284.6-358.5 g) and body length(15.9-21.8 cm) were collected from the South China Sea from Aug., 2015 to Aug., 2016. In general, skin and muscle possessed negligible fat contents(0.3-0.4 g/100 g wet weight) and low energy value(317.4-497.3 k J/100 g), while proteins(17.8-25.4 g/100 g wet weight) were found to be the richest component. The dominant amino acids in both muscle and skin of D. hystrix included histidine, cysteine, tyrosine, isoleucine, leucine and lysine, while the major mineral elements were calcium, magnesium, potassium,sodium, and phosphorus. It was apparent that D. hystrix had high content of amino acids, and the rate of essentia amino acids was accorded with FAO/WHO standard. This study illustrated that the wild D. hystrix would be a valuable natural source of protein, with high commercial potential, and especially, skin and muscle were more valuable. Moreover, the basic data from the present study would supply the fundamental reference for formulated diet and culture of this species.展开更多
Background: Over the last few years sunscreen products have been suspected to be harmful to corals, especially because of their putative negative impact on symbiotic microalgae housed by these cnidarians. Previous pub...Background: Over the last few years sunscreen products have been suspected to be harmful to corals, especially because of their putative negative impact on symbiotic microalgae housed by these cnidarians. Previous publications reported that minerals or chemical UV filters could induce the release of microalgae from corals inducing their bleaching. The study of the ecotoxicity of finished cosmetic products containing these filters is important. Objectives: We sought to assess ex vivo the toxicity of five emulsions containing UV-filters on coral cuttings of Seriatopora hystrix. Materials and Methods: Coral cuttings were put in contact with 5 different emulsions containing UV-filters. The toxicity readout was the ability to induce polyp retraction and/or fragment bleaching of the coral cuttings of Seriatopora hystrix. Results: In our experimental conditions, none of the five tested formulas neither induced any significant polyp retraction nor triggered fragment bleaching of the coral. Conclusions: The five tested emulsions containing UV-filters did not modify coral cuttings. In vivo, larger tests are necessary to verify the results of this ex vivo pilot study.展开更多
TheÇorakyerler fossil locality in Anatolia reveals unique faunal elements.This study introduces Hystrix kayae sp.nov.,a new Late Miocene porcupine initially classified as Hystrix sp.Yavuz et al.,2018.This finding...TheÇorakyerler fossil locality in Anatolia reveals unique faunal elements.This study introduces Hystrix kayae sp.nov.,a new Late Miocene porcupine initially classified as Hystrix sp.Yavuz et al.,2018.This finding expands our knowledge of Late Miocene Anatolian porcupines,bringing the total number of known species to three.H.kayae sp.nov.is larger than Hystrix aryanensis and exhibits greater upper cheek tooth crown height.Its cheek tooth morphology differs from Hystrix depereti,and it possesses distinctive U-shaped choanae unlike Hystrix primigenia.TheÇorakyerler locality may predate sites with H.aryanensis and H.primigenia but aligns temporally with Hystrix parvae localities.This study enhances our understanding of Late Miocene porcupine diversity in Anatolia,emphasizing the importance ofÇorakyerler in unraveling the evolutionary history of these fascinating mammals.展开更多
This research study evaluated the effect of different additives on the bait consumption by Indian crested porcupine,a serious forest and agricultural pest,under field conditions.Different additives(saccharin,common sa...This research study evaluated the effect of different additives on the bait consumption by Indian crested porcupine,a serious forest and agricultural pest,under field conditions.Different additives(saccharin,common salt,bone meal,fish meal,peanut butter,egg yolk,egg shell powder,yeast powder,mineral oil and coconut oil)at 2 and 5%each were tested for their relative preference,using groundnut-maize(1:1)as basic bait.All the additives were tested under a no-choice test pattern.For control tests,no additive was mixed with the basic bait.Saccharin at 5%concentration significantly enhanced the consumption of bait over the basic bait,while 2%saccharin supplemented bait resulted in a non-significant bait consumption.All other additives did not enhance the consumption of the bait material;rather,these worked as repellents.However,the repellency was lowest with the common salt,followed by egg yolk,egg shell powder,bone meal,peanut butter,mineral oil,fish meal and yeast powder,while coconut remained the most repellent compound.The present study suggested that groundnut-maize(1:1)supplemented with 5%saccharin was the preferred bait combination,and can be used with different rodenticides for the management of Indian crested porcupine.展开更多
文摘野黄瓜(Cucum is hystrix,2n=24)为短日照植物,因其与栽培黄瓜(C.sativus,2n=14)花期不遇等杂交障碍的存在而使种间杂交难以顺利进行。对野黄瓜短光照处理40 d以上,使得两者花期相遇,成功地进行了野黄瓜与3种不同基因型栽培黄瓜(“二早子”、“长春密刺”和“NC4406”)的正反杂交,从中获得了3种杂交组合的幼胚。采用改良的方法对这3种基因型杂种胚进行胚胎拯救,再生频率达80%以上,其杂种的真实性通过植物学性状观察、体细胞染色体计数和天冬氨酸转氨酶(AAT)同工酶分析得到了证实。研究中还发现不同基因型杂种F1的育性存在明显差异,其中以C.hystrixדNC4406”育性最高,花粉可染率达23.3%,是在二倍体水平上进行育种利用的良好材料。
基金supported by Agriculture and Food Research Initiative Competitive Grant 2013-67013-21105 from the U.S. Department of Agriculture National Institute of Food to YWthe National Natural Science Foundation of China to YH (No. 31271350)
文摘Interspecific hybridization and allopolyploidization contribute to the improvement of many important crops. Recently, we successfully developed an amphidiploid from an interspecific cross between cucumber(Cucumis sativus, 2n = 2x = 14) and its relative C. hystrix(2n = 2x = 24) followed by chemical induction of chromosome doubling. The resulting allotetraploid plant was self-pollinated for three generations. The fertility and seed set of the amphidiploid plants were very low. In this study, we investigated the meiotic chromosome behavior in pollen mother cells with the aid of fluorescence in situ hybridization, aiming to identify the reasons for the low fertility and seed set in the amphidiploid plants. Homologous chromosome pairing appeared normal, but chromosome laggards were common, owing primarily to asynchronous meiosis of chromosomes from the two donor genomes. We suggest that asynchronous meiotic rhythm between the two parental genomes is the main reason for the low fertility and low seed set of the C. hystrix–cucumber amphidiploid plants.
基金The authors would like to thank Dr.T.Sivakumar,Principal,Nandha College of Pharmacy and Research Institute,Erode,TN,India for necessary permission to carry out the study and Dr.S.Sengottuvelu,Head,Department of Pharmacology,and Mrs.V.Lalitha,Department of Pharmacology,Nandha College of Pharmacy and Research Institute for their support and suggestions.
基金Supported by DP2M DIKT1,Ministry of Education,Republic of Indoncsia through PKMP 2012(Grant No.0071/F.5.3/KPM/2012/5184)
文摘Objective:To observe the combination effect of doxorubicin and Citrus hystrix(kaffir lime's)peel ethanolic extract(ChEE)on blood serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)activity and cardio-hepato-histopathology of female Sprague Dawley rats.Methods:Doxorubicin and ChEE(5 rats per group)were administered in five groups of 3 rats each for 11 d.Group I:doxorubicin(dox)4.67 mg/kg body weight;Group II:dox+ChEE 500 mg/kg body weight;Group HI:dox+ChEE 1000 mg/kg body weight;Group IV:ChEE 1000 mg/kg body weight;Group V:untreated(control).Results:ChEE repaired cardiohistopathology profile of doxorubicin induced cardiotoxicity and hepatotoxicity rats,but did not repair neither hepatohistopathology profile nor reduce serum activity of ALT and AST.Conclusion:ChEE has potency to be developed as cardioprotector agent in chemotherapy.
基金Provincial Science and Technology Research Project of Guangdong(2014KJcx022).
文摘Both the stoichiometric characteristics and leaf functional traits can reflect the adaptability of plants to changes in the external living environment.In particular,for varying aged forest plants,the study of stoichiometric characteristics and leaf functional traits can reflect the plant’s life history strategy and its resource investment and allocation methods.In this paper,the 1.5-year-old,5-year-old,15-year-old and 20-year-old Castanopsis hystrix were selected as the research objects to investigate the trends of stoichiometry and leaf functional traits,and their synergistic changes were verified.The results showed that with the increase of age,the stability of C.hystrix stoichiometry gradually increased,and the functional traits also tended to invest in security.However,there was no synergistic change between the two,which might be attributed to the different sensitivity of the C.hystrix leaf to the environment at the life history level of 1.5-year-old to 20-year-old.
基金This paper is translated from its Chinese version in Scientia Agricultura Sinica.This research was partially supported by the Transcentury Training Program Foundation for the Talents by the Ministry of Education of China to Dr.Chen Jinfeng(30470120)by the National Natural Science Foundation of China(30671419)+2 种基金the National Hi-Tech R&D Program(2004AA241120)the Tang Foundation Cornell-China Scholar Programthe Pickle Seed Research Foundation of Pickle Packers International.The authors sincerely thank Dr.Zhai Huqu,the President of the Chinese Academy of Agricultural Sciences for his support in this research.
文摘An interspecific hybrid F1 of Cucumis hystrix Chakr. x Cucumis sativus L. （NC4406） was used to establish the developmental sequence and to characterize the male and female gametophytes at cytological level for further understanding of the phylogenic relationship and the mechanism of fertility or sterility in the interspecific hybrid F1 The development of male and female gametophytes was studied through meiotic analysis and paraffin section observation technique, respectively. Meanwhile, the fertility level was assessed through hybrid F1 backcrossing to cultivated cucumber 4406. Variable chromosome configurations were observed in the pollen mother cells （PMCs） of hybrid F1 at metaphase Ⅰ , e.g., univalents, bivalents, trivalents, quadravalents, etc. At anaphase Ⅰ and Ⅱ, chromosome lagging and bridges were frequently observed as well, which led to the formation of polyads and only a partial number of microspores could develop into fertile pollen grains （about 23.3%）. Observations of the paraffin sections showed numerous degenerated and abnormal embryo sacs during the development of female gametophytes, and only 40% of the female gametophytes could develop into normal eight-nuclear megaspore. On an average, 22.8 and 6.3 seeds per fruit could be obtained from the reciprocal backcross. The interspecific hybrid F1 of C. hystrix x NC4406 was partially fertile; however, the meiotic behaviors of hybrid F1 showed a high level of intergenomic recombination between C. hystrix and C. sativus chromosomes, which indicated that it plays an important role for introgression of useful traits from C. hystrix into C. sativus.
基金Supported by Major Science and Technology Projects from Science and Technology Department of Hainan Province,China(ZDKJ2016009ZDKJ2016013)+1 种基金Natural Science Foundation of Hainan Province(20163074)Key laboratory of Seawater Aquaculture Research of Sanya City(L1507)
文摘Nutrient compositions including proximate, fatty acids, amino acids, minerals and vitamins in tissues of the wild spot-fin porcupinefish(Diodon hystrix) were analyzed in detail to assess their nutrient value and to further explore the dietary requirements of this species farmed. Diodon hystrix individuals with similar body weight(284.6-358.5 g) and body length(15.9-21.8 cm) were collected from the South China Sea from Aug., 2015 to Aug., 2016. In general, skin and muscle possessed negligible fat contents(0.3-0.4 g/100 g wet weight) and low energy value(317.4-497.3 k J/100 g), while proteins(17.8-25.4 g/100 g wet weight) were found to be the richest component. The dominant amino acids in both muscle and skin of D. hystrix included histidine, cysteine, tyrosine, isoleucine, leucine and lysine, while the major mineral elements were calcium, magnesium, potassium,sodium, and phosphorus. It was apparent that D. hystrix had high content of amino acids, and the rate of essentia amino acids was accorded with FAO/WHO standard. This study illustrated that the wild D. hystrix would be a valuable natural source of protein, with high commercial potential, and especially, skin and muscle were more valuable. Moreover, the basic data from the present study would supply the fundamental reference for formulated diet and culture of this species.
文摘Background: Over the last few years sunscreen products have been suspected to be harmful to corals, especially because of their putative negative impact on symbiotic microalgae housed by these cnidarians. Previous publications reported that minerals or chemical UV filters could induce the release of microalgae from corals inducing their bleaching. The study of the ecotoxicity of finished cosmetic products containing these filters is important. Objectives: We sought to assess ex vivo the toxicity of five emulsions containing UV-filters on coral cuttings of Seriatopora hystrix. Materials and Methods: Coral cuttings were put in contact with 5 different emulsions containing UV-filters. The toxicity readout was the ability to induce polyp retraction and/or fragment bleaching of the coral cuttings of Seriatopora hystrix. Results: In our experimental conditions, none of the five tested formulas neither induced any significant polyp retraction nor triggered fragment bleaching of the coral. Conclusions: The five tested emulsions containing UV-filters did not modify coral cuttings. In vivo, larger tests are necessary to verify the results of this ex vivo pilot study.
文摘TheÇorakyerler fossil locality in Anatolia reveals unique faunal elements.This study introduces Hystrix kayae sp.nov.,a new Late Miocene porcupine initially classified as Hystrix sp.Yavuz et al.,2018.This finding expands our knowledge of Late Miocene Anatolian porcupines,bringing the total number of known species to three.H.kayae sp.nov.is larger than Hystrix aryanensis and exhibits greater upper cheek tooth crown height.Its cheek tooth morphology differs from Hystrix depereti,and it possesses distinctive U-shaped choanae unlike Hystrix primigenia.TheÇorakyerler locality may predate sites with H.aryanensis and H.primigenia but aligns temporally with Hystrix parvae localities.This study enhances our understanding of Late Miocene porcupine diversity in Anatolia,emphasizing the importance ofÇorakyerler in unraveling the evolutionary history of these fascinating mammals.
基金supported by Higher Education Commission of Pakistanthrough a financial support provided under its Indigenous PhD Fellowship Programme to Muhammad Mushtaq.
文摘This research study evaluated the effect of different additives on the bait consumption by Indian crested porcupine,a serious forest and agricultural pest,under field conditions.Different additives(saccharin,common salt,bone meal,fish meal,peanut butter,egg yolk,egg shell powder,yeast powder,mineral oil and coconut oil)at 2 and 5%each were tested for their relative preference,using groundnut-maize(1:1)as basic bait.All the additives were tested under a no-choice test pattern.For control tests,no additive was mixed with the basic bait.Saccharin at 5%concentration significantly enhanced the consumption of bait over the basic bait,while 2%saccharin supplemented bait resulted in a non-significant bait consumption.All other additives did not enhance the consumption of the bait material;rather,these worked as repellents.However,the repellency was lowest with the common salt,followed by egg yolk,egg shell powder,bone meal,peanut butter,mineral oil,fish meal and yeast powder,while coconut remained the most repellent compound.The present study suggested that groundnut-maize(1:1)supplemented with 5%saccharin was the preferred bait combination,and can be used with different rodenticides for the management of Indian crested porcupine.
