Wistar rats were inoculated with purified YWK-I antibody.The anti-idiotypic antibodies were isolated from rat sera by successive passage over affinity chromatography columns of YWK-I mAb and normal mouse Igs.Specifici...Wistar rats were inoculated with purified YWK-I antibody.The anti-idiotypic antibodies were isolated from rat sera by successive passage over affinity chromatography columns of YWK-I mAb and normal mouse Igs.Specificity of anti-Id antibody was established by ELISA.The 84kD protein inhibited the binding of anti-Id to YWK-I mAb,but failed to repress antibody against normal mouse Ig binding to YWK-I mAb.In competitive inhibition assay,84kD protein had shown the ability to compete with anti-Id binding to YWK-I mAb in a dose-dependent manner.Crude sperm extract showed a lower competitive ability.No effect was found with the irrelevant 36kD sperm protein.The antisera from the Balb/C micr immunized with AId contained Ab3 that reacted with 84kD sperm protein.The binding of anti-Id to YWK-I mAb was inhibited by Ab3 in a dose-dependent fashion and Ab3 was shown to be able to induce human sperm agglutination.These results indicate that anti-Id which may mimic an epitope of the 84kD protein could be exploited as an antigen to raise antibodies against sperm protein.展开更多
2D MBenes have been theoretically predicted to possess unique electronic structures and physicochemical properties,and thus shown great promise in various applications.However,the synthesis of individual single-layer ...2D MBenes have been theoretically predicted to possess unique electronic structures and physicochemical properties,and thus shown great promise in various applications.However,the synthesis of individual single-layer MBene remains a grand challenge due to its orthorhombic structure of MAB phases.Recently,scientists from Link?ping University have fabricated 2D monolayer Mo4/3B2-xTzwith ordered metal vacancies.Their results demonstrated the feasibility of top-down approach by chemical exfoliation of laminated compounds and provided the principle for further preparation of a wealth of MBenes.展开更多
文摘Wistar rats were inoculated with purified YWK-I antibody.The anti-idiotypic antibodies were isolated from rat sera by successive passage over affinity chromatography columns of YWK-I mAb and normal mouse Igs.Specificity of anti-Id antibody was established by ELISA.The 84kD protein inhibited the binding of anti-Id to YWK-I mAb,but failed to repress antibody against normal mouse Ig binding to YWK-I mAb.In competitive inhibition assay,84kD protein had shown the ability to compete with anti-Id binding to YWK-I mAb in a dose-dependent manner.Crude sperm extract showed a lower competitive ability.No effect was found with the irrelevant 36kD sperm protein.The antisera from the Balb/C micr immunized with AId contained Ab3 that reacted with 84kD sperm protein.The binding of anti-Id to YWK-I mAb was inhibited by Ab3 in a dose-dependent fashion and Ab3 was shown to be able to induce human sperm agglutination.These results indicate that anti-Id which may mimic an epitope of the 84kD protein could be exploited as an antigen to raise antibodies against sperm protein.
基金supported by the National Natural Science Foundation of China(Nos.52172205,11804166)the China Postdoctoral Science Foundation(No.2018M630587)Tianjin Natural Science Foundation(No.18JCQNJC77100)。
文摘2D MBenes have been theoretically predicted to possess unique electronic structures and physicochemical properties,and thus shown great promise in various applications.However,the synthesis of individual single-layer MBene remains a grand challenge due to its orthorhombic structure of MAB phases.Recently,scientists from Link?ping University have fabricated 2D monolayer Mo4/3B2-xTzwith ordered metal vacancies.Their results demonstrated the feasibility of top-down approach by chemical exfoliation of laminated compounds and provided the principle for further preparation of a wealth of MBenes.
