Feasibility of iFISH patterns in hematologic malignancies among Congolese patients at Kinshasa University clinics

Objective: To analyze the feasibility of detecting Ph1 in leukemia patients in the Kinshasa University Clinics in the Democratic Republic of Congo, at KU Leuven, Belgium.Methods: Bone marrow and peripheral blood samples with chronic myeloid leukemia,acute myeloid leukemia or acute leukocytes leukemia were obtained from 32 patients in Kinshasa University clinics in the Democratic Republic of Congo and transferred to KU Leuven in Belgium for i FISH feasibility. Ph^1 was detected by using a remote analysis of interphase fluorescence in situ hybridization(i FISH).Results: Out of the 32 patients involved in this study, 65.6%(n = 21) of the cases were successfully tested, of which 52.4%(n = 11) were i FISH positives for the variant t(9;22)(presence of Ph^1) in chronic myeloid leukemia samples and 47.6%(n = 10) negatives in all subtypes of hematological malignancies. However, there was a female predominance in chronic myeloid leukemia samples Ph^1-positives by i FISH, whereas no sexual influence was observed on acute subtypes of leukemia.Conclusions: i FISH analysis is feasible on samples obtained from remote sites in the Democratic Republic of Congo. However, the optimization of the sample storage is necessary to further improve i FISH's performance.

电子宠物

电子宠物狗“idog”已经给世嘉尝到了甜头，今夏则乘胜出击。“idog”的新款变成了五彩。新品的电子宠物则是“鱼”ifish，除了原有的音乐功能之外，ifish转动时其柔软的身体会像在水中游动一样缓缓飘摆。

不同方法检测乳腺癌循环肿瘤细胞的诊断价值研究

以一年内临床收治的乳腺癌和乳腺纤维瘤患者为研究对象,同期健康者为对照,利用微流控免疫磁珠筛选+免疫荧光检测法(Microfluidic-beads)、差异富集-荧光免疫染色原位杂交技术(SE-iFISH)、RNA原位杂交法(RNA-FISH)3种方法对研究对象外周血循环肿瘤细胞(CTCs)进行检测,比较观察组和对照组CTCs阳性检出率的差异,探究不同检测方法对乳腺癌患者的CTCs检测效果及诊断价值。结果表明:采用SE-iFISH法和RNA-FISH法检测时,观察组患者阳性检出率较高,分别为88%和100%。但采用Microfluidic-beads法时,观察组患者阳性检出率较低,仅为48%。基于CTCs的检测表征乳腺癌方面,SE-iFISH法和RNA-FISH法具有较高的灵敏度及特异性。这一研究对后期乳腺癌患者的临床诊断与治疗具有一定的指导意义。

差相富集-免疫荧光原位杂交技术检测膀胱癌患者循环肿瘤细胞的初步研究

目的利用差相富集-免疫荧光原位杂交技术(subtraction enrichment and immunostainingfluorescence in situ hybridization,SE-iFISH)检测膀胱癌患者外周血循环肿瘤细胞(circulating tumor cells,CTCs),并探究该检测结果与肿瘤临床特征的关系。方法收集2016年4~6月收治入院的16例膀胱癌患者(实验组)临床病理资料,其中男14例,女2例,年龄(66.88±8.97)岁。另取10例非肿瘤患者为对照(对照组),其中男7例,女3例,年龄(61.90±9.13)岁。取外周静脉血各6mL,差相富集法收集外周血CTCs,肿瘤细胞标志物-iFISH检测6mL血中所有CTCs的数量及相关生物学特性,比较CTCs的数量与膀胱癌临床特征(有无血尿、肿瘤大小、数目、是否侵及肌层、原发肿瘤分期、淋巴结转移、肿瘤级别、肿瘤病理学类型等)的关系。结果实验组CTCs阳性检出率为43.75%(7/16),对照组CTCs阳性检出率为0%(0/10),两组阳性检出率差异有统计学意义(P=0.022 7)。实验组CTCs含量为0~29个,中位数为0个,对照组CTCs含量为0个,两组CTCs含量差异有统计学意义(P=0.028 3)。实验组中,有无血尿、肿瘤大小、数目、是否侵及肌层、原发肿瘤分期、淋巴结转移、肿瘤级别、肿瘤病理学类型等亚组之间,CTCs阳性检出率的差异无统计学意义。结论 SE-iFISH检测膀胱癌外周血CTCs可行性好。关于CTCs含量与膀胱癌患者临床特征的关系尚不肯定,需更大样本量予以说明。