目的采用非对称采集与迭代最小二乘估算法迭代水脂分离(iteraterative decomposition of water and fat with echo asymmetry and least-squares estimation quantitation,IDEAL-IQ)方法定量评估冈上肌腱损伤的严重程度与肩袖肌群脂肪...目的采用非对称采集与迭代最小二乘估算法迭代水脂分离(iteraterative decomposition of water and fat with echo asymmetry and least-squares estimation quantitation,IDEAL-IQ)方法定量评估冈上肌腱损伤的严重程度与肩袖肌群脂肪浸润程度及受试者特征之间的关系。材料与方法回顾性分析2022年8月至2024年6月本院经肩关节镜证实的33例冈上肌腱部分撕裂患者及89例完全撕裂患者,均进行了常规MRI扫描及IDEAL-IQ序列扫描。由两名放射科医生分别对所有受试者的MRI图像进行独立评估,根据常规MRI图像的冈上肌腱损伤表现,将完全撕裂组的冈上肌腱按照Patte分型分为Patte 1型(Ⅱ级)、Patte 2型(Ⅲ级)、Patte 3型(Ⅳ级),将部分撕裂组定义为Ⅰ级。同时在斜矢状位上进行Goutallier分级及Thomazeau萎缩分级,并通过GE ADW 4.7工作站后处理软件在IDEAL-IQ序列生成的脂肪分数图像上测量冈上肌、冈下肌、肩胛下肌及小圆肌脂肪分数(fat fraction,FF)。用组内相关系数(intra-class correlation coefficient,ICC)及Kappa一致性检验评估观察者间及观察者内的一致性。采用Kruskal-Wallis H检验、单因素ANOVA检验分析FF值在不同分组之间的差异,组间两两比较用Bonferroni检验。采用Pear_(s)on相关性分析肩袖肌肉FF值与年龄、症状持续时间的相关性(相关系数r),Spearman相关性分析冈上肌腱损伤分级与肩袖肌群FF值、Goutallier分级及Thomazeau萎缩分级之间的相关性(相关系数r_(s))。结果(1)冈上肌、冈下肌、肩胛下肌的FF值在冈上肌腱损伤Ⅳ级中显著高于Ⅲ级,高于Ⅱ级和Ⅰ级,差异有统计学意义(P值分别为<0.001、<0.001、0.005);小圆肌的FF值在不同分级之间差异无统计学意义(P=0.073)。组内比较Ⅰ级和Ⅱ级的冈上肌、冈下肌、肩胛下肌、小圆肌FF值差异无统计学意义(P值分别为0.026、0.102);Ⅲ级和Ⅳ级的FF值差异有统计学意义(P<0.001)。(2)冈上肌、冈下肌、小圆肌的FF值与年龄呈中等相关(r值分别为0.381、0.339、0.349,P均<0.001),肩胛下肌的FF值与年龄呈弱相关(r=0.216,P=0.017);冈上肌、冈下肌、肩胛下肌FF值与症状持续时间呈中等程度相关(r分别为0.442、0.412、0.314,P均<0.001),小圆肌的FF值与症状持续时间呈弱相关(r=0.277,P=0.002);冈上肌腱损伤程度与冈上肌FF值呈显著相关(r_(s)=0.740,P<0.001),与冈下肌的FF值呈强相关性(r_(s)=0.596,P<0.001),与肩胛下肌、小圆肌的FF值呈弱相关(r_(s)分别为0.257、0.212,P值分别为0.004、0.019);冈上肌损伤程度分级与Goutallier分级、Thomazeau分级之间呈显著正相关(r_(s)分别为0.757、0.737,P均<0.001),且冈上肌FF值在Goutallier和Thomazeau的分级中差异具有统计学意义(P均<0.001)。结论3.0 T MR IDEAL-IQ序列能量化和客观评估肩袖肌群脂肪浸润程度,肩袖肌群脂肪浸润程度与冈上肌腱损伤分级呈正相关,与年龄、症状持续时间呈正相关。展开更多
目的 采用定量非对称回波的最小二乘估算法迭代水脂分离序列(iterative decomposition of water and fat with echo asymmetry and least-squares estimation quantitation sequence, IDEAL-IQ)联合计算机显微断层扫描(micro computed t...目的 采用定量非对称回波的最小二乘估算法迭代水脂分离序列(iterative decomposition of water and fat with echo asymmetry and least-squares estimation quantitation sequence, IDEAL-IQ)联合计算机显微断层扫描(micro computed tomography, Micro-CT)技术评估兔1型糖尿病腰椎骨髓脂肪含量和骨小梁微结构相关性。材料与方法 将16只雄性日本大耳白兔随机分为糖尿病组(n=8)和对照组(n=8),糖尿病组采用四氧嘧啶造模。在造模成功后第0、4、8、12、16周通过IDEAL-IQ定量评估兔腰椎骨髓脂肪含量。在第16周采用Micro-CT及组织病理学检查对兔腰椎骨小梁微结构进行定量评估。不同时间点腰椎IDEAL-IQ脂肪分数差异比较采用重复测量方差分析;同一时间点组间脂肪分数、Micro-CT骨小梁形态计量学参数、组织病理学参数差异比较采用独立样t检验或Mann-Whitney U检验;采用Pearson相关系数评估骨髓脂肪分数与骨小梁形态计量学参数、组织病理学参数相关性。结果 糖尿病组骨髓脂肪含量随时间变化呈上升趋势,且差异存在统计学意义(P<0.05),而对照组各时间点脂肪含量差异无统计学意义(P>0.05);糖尿病组与对照组腰椎骨髓脂肪分数在第16周差异有统计学意义(59.987±4.859 vs. 51.015±8.469,P<0.05);第16周糖尿病组骨体积、总表面积、骨表面积、骨表面积总体积比、骨小梁数量均低于对照组,而骨小梁分离度高于对照组,差异有统计学意义(P<0.05);组织病理学结果显示,与对照组相比,第16周糖尿病兔腰椎骨髓脂肪细胞数量(32.875±11.051 vs. 71.667±13.125, P<0.01)增加,而骨小梁数量(11.375±1.506vs.4.333±1.211, P<0.01)与骨小梁面积(0.927±0.071vs.0.312±0.100, P<0.01)明显减少;IDEAL-IQ脂肪分数与组织病理学脂肪细胞计数呈正相关关系(r=0.539 95%CI:0.012~0.832),与骨小梁模式因子、骨小梁结构模型指数、骨小梁分离度呈轻中度正相关关系,与骨矿物质密度、总体积、骨体积、骨体积分数、总表面积、骨表面积、骨表面积体积比、骨表面积总体积比、骨小梁数量呈轻至中度负相关关系。结论 1型糖尿病腰椎骨髓脂肪含量与骨小梁微结构变化存在相关性,IDEAL-IQ联合Micro-CT的多模态定量影像技术有望为1型糖尿病提供全面的骨髓微环境评估策略。展开更多
IQ67-domain(IQD)proteins function in plant defense and in organ development.The mechanisms by which they influence cotton fiber development are unknown.In the present study,GhIQD10 was expressed mainly in the transiti...IQ67-domain(IQD)proteins function in plant defense and in organ development.The mechanisms by which they influence cotton fiber development are unknown.In the present study,GhIQD10 was expressed mainly in the transition period of cotton fiber development,and GhIQD10-overexpression lines showed shorter fibers.GhIQD10 interacted with GhCaM7 and the interaction was inhibited by Ca^(2+).In in vitro ovule culture,Ca^(2+)rescued the shorter-fiber phenotype of GhIQD10-overexpression lines,which were insensitive to the Ca^(2+)channel inhibitor verapamil and the Ca^(2+)pool release channel blocker 2-aminoethoxydiphenyl borate.We conclude that GhIQD10 affects cotton fiber elongation via Ca^(2+)signaling by interacting with GhCaM7.Brassinosteroid(BR)biosynthesis and signaling genes were up-regulated in GhIQD10-overexpression lines.Fiber development in these lines was not affected by epibrassinolide or the BR biosynthesis inhibitor brassinozole,indicating that the influence of GhIQD10 on fiber elongation was not associated with BR.展开更多
文摘目的采用非对称采集与迭代最小二乘估算法迭代水脂分离(iteraterative decomposition of water and fat with echo asymmetry and least-squares estimation quantitation,IDEAL-IQ)方法定量评估冈上肌腱损伤的严重程度与肩袖肌群脂肪浸润程度及受试者特征之间的关系。材料与方法回顾性分析2022年8月至2024年6月本院经肩关节镜证实的33例冈上肌腱部分撕裂患者及89例完全撕裂患者,均进行了常规MRI扫描及IDEAL-IQ序列扫描。由两名放射科医生分别对所有受试者的MRI图像进行独立评估,根据常规MRI图像的冈上肌腱损伤表现,将完全撕裂组的冈上肌腱按照Patte分型分为Patte 1型(Ⅱ级)、Patte 2型(Ⅲ级)、Patte 3型(Ⅳ级),将部分撕裂组定义为Ⅰ级。同时在斜矢状位上进行Goutallier分级及Thomazeau萎缩分级,并通过GE ADW 4.7工作站后处理软件在IDEAL-IQ序列生成的脂肪分数图像上测量冈上肌、冈下肌、肩胛下肌及小圆肌脂肪分数(fat fraction,FF)。用组内相关系数(intra-class correlation coefficient,ICC)及Kappa一致性检验评估观察者间及观察者内的一致性。采用Kruskal-Wallis H检验、单因素ANOVA检验分析FF值在不同分组之间的差异,组间两两比较用Bonferroni检验。采用Pear_(s)on相关性分析肩袖肌肉FF值与年龄、症状持续时间的相关性(相关系数r),Spearman相关性分析冈上肌腱损伤分级与肩袖肌群FF值、Goutallier分级及Thomazeau萎缩分级之间的相关性(相关系数r_(s))。结果(1)冈上肌、冈下肌、肩胛下肌的FF值在冈上肌腱损伤Ⅳ级中显著高于Ⅲ级,高于Ⅱ级和Ⅰ级,差异有统计学意义(P值分别为<0.001、<0.001、0.005);小圆肌的FF值在不同分级之间差异无统计学意义(P=0.073)。组内比较Ⅰ级和Ⅱ级的冈上肌、冈下肌、肩胛下肌、小圆肌FF值差异无统计学意义(P值分别为0.026、0.102);Ⅲ级和Ⅳ级的FF值差异有统计学意义(P<0.001)。(2)冈上肌、冈下肌、小圆肌的FF值与年龄呈中等相关(r值分别为0.381、0.339、0.349,P均<0.001),肩胛下肌的FF值与年龄呈弱相关(r=0.216,P=0.017);冈上肌、冈下肌、肩胛下肌FF值与症状持续时间呈中等程度相关(r分别为0.442、0.412、0.314,P均<0.001),小圆肌的FF值与症状持续时间呈弱相关(r=0.277,P=0.002);冈上肌腱损伤程度与冈上肌FF值呈显著相关(r_(s)=0.740,P<0.001),与冈下肌的FF值呈强相关性(r_(s)=0.596,P<0.001),与肩胛下肌、小圆肌的FF值呈弱相关(r_(s)分别为0.257、0.212,P值分别为0.004、0.019);冈上肌损伤程度分级与Goutallier分级、Thomazeau分级之间呈显著正相关(r_(s)分别为0.757、0.737,P均<0.001),且冈上肌FF值在Goutallier和Thomazeau的分级中差异具有统计学意义(P均<0.001)。结论3.0 T MR IDEAL-IQ序列能量化和客观评估肩袖肌群脂肪浸润程度,肩袖肌群脂肪浸润程度与冈上肌腱损伤分级呈正相关,与年龄、症状持续时间呈正相关。
文摘目的 采用定量非对称回波的最小二乘估算法迭代水脂分离序列(iterative decomposition of water and fat with echo asymmetry and least-squares estimation quantitation sequence, IDEAL-IQ)联合计算机显微断层扫描(micro computed tomography, Micro-CT)技术评估兔1型糖尿病腰椎骨髓脂肪含量和骨小梁微结构相关性。材料与方法 将16只雄性日本大耳白兔随机分为糖尿病组(n=8)和对照组(n=8),糖尿病组采用四氧嘧啶造模。在造模成功后第0、4、8、12、16周通过IDEAL-IQ定量评估兔腰椎骨髓脂肪含量。在第16周采用Micro-CT及组织病理学检查对兔腰椎骨小梁微结构进行定量评估。不同时间点腰椎IDEAL-IQ脂肪分数差异比较采用重复测量方差分析;同一时间点组间脂肪分数、Micro-CT骨小梁形态计量学参数、组织病理学参数差异比较采用独立样t检验或Mann-Whitney U检验;采用Pearson相关系数评估骨髓脂肪分数与骨小梁形态计量学参数、组织病理学参数相关性。结果 糖尿病组骨髓脂肪含量随时间变化呈上升趋势,且差异存在统计学意义(P<0.05),而对照组各时间点脂肪含量差异无统计学意义(P>0.05);糖尿病组与对照组腰椎骨髓脂肪分数在第16周差异有统计学意义(59.987±4.859 vs. 51.015±8.469,P<0.05);第16周糖尿病组骨体积、总表面积、骨表面积、骨表面积总体积比、骨小梁数量均低于对照组,而骨小梁分离度高于对照组,差异有统计学意义(P<0.05);组织病理学结果显示,与对照组相比,第16周糖尿病兔腰椎骨髓脂肪细胞数量(32.875±11.051 vs. 71.667±13.125, P<0.01)增加,而骨小梁数量(11.375±1.506vs.4.333±1.211, P<0.01)与骨小梁面积(0.927±0.071vs.0.312±0.100, P<0.01)明显减少;IDEAL-IQ脂肪分数与组织病理学脂肪细胞计数呈正相关关系(r=0.539 95%CI:0.012~0.832),与骨小梁模式因子、骨小梁结构模型指数、骨小梁分离度呈轻中度正相关关系,与骨矿物质密度、总体积、骨体积、骨体积分数、总表面积、骨表面积、骨表面积体积比、骨表面积总体积比、骨小梁数量呈轻至中度负相关关系。结论 1型糖尿病腰椎骨髓脂肪含量与骨小梁微结构变化存在相关性,IDEAL-IQ联合Micro-CT的多模态定量影像技术有望为1型糖尿病提供全面的骨髓微环境评估策略。
基金funded by the National Natural Science Foundation of China(31571722 and 31971984).
文摘IQ67-domain(IQD)proteins function in plant defense and in organ development.The mechanisms by which they influence cotton fiber development are unknown.In the present study,GhIQD10 was expressed mainly in the transition period of cotton fiber development,and GhIQD10-overexpression lines showed shorter fibers.GhIQD10 interacted with GhCaM7 and the interaction was inhibited by Ca^(2+).In in vitro ovule culture,Ca^(2+)rescued the shorter-fiber phenotype of GhIQD10-overexpression lines,which were insensitive to the Ca^(2+)channel inhibitor verapamil and the Ca^(2+)pool release channel blocker 2-aminoethoxydiphenyl borate.We conclude that GhIQD10 affects cotton fiber elongation via Ca^(2+)signaling by interacting with GhCaM7.Brassinosteroid(BR)biosynthesis and signaling genes were up-regulated in GhIQD10-overexpression lines.Fiber development in these lines was not affected by epibrassinolide or the BR biosynthesis inhibitor brassinozole,indicating that the influence of GhIQD10 on fiber elongation was not associated with BR.
