Fluorescence of Tb-Ibuprofenum-Phen Complex and New Tissue Staining Method

A new complex Tb(IB)_3(phen)·2H_2O that gives off green fluorescence was synthesized. The results show that the strong fluorescence emitting of 490 and 545 nm for Tb 3+ in the Tb(IB)_3(phen)·2H_2O complex is related to the transitions 5D_4-7F_6 and 5D_4-7F_5, respectively. The results indicate that the complex is concentrated on the nuclei regions in the section of onion toot tip tissues and the nuclei are high lighted under fluorescent microscope. It is possible that the Tb(IB)_3(phen)·2H_2O complex can be developed as a fluorescent dye in biological and pathological studies.

Synthesis and Fluorescence of Eu-Ibuprofenum-Phen Complex and Determination of ATP by Fluorescence Quenching of Complex

The complex Eu(IB)_3(phen)·2H_2O was synthesized. The results show that the strong fluorescence emitting of 593 and 615 nm for Eu^(3+) in the Eu(IB)_3(phen) complex is related to the transitions (()~5D_0-()~7F_1) and (()~5D_0-()~7F_2,) respectively. A sensitive method for the determination of adenosine disodium triphosphate (ATP) by fluorescence quenching is proposed. The method is based on the ability of ATP to inhibit the formation of a strong fluorescent complex of Eu(IB)_3(phen). In conditions of pH 6.7 HAc-NaAc buffer, λ_(ex)=273 nm and λ_(em)=615 nm, the linear range of the determination was 0.1～10 μg·ml^(-1) and the detection limit was 0.03 μg·ml^(-1) for ATP. The method has been applied to determine ATP in adenosine disodium triphosphate tablets samples with relative standard deviation of 2.18% and recovery of (98.4%～)104.0%.

高效液相色谱法测定复方美沙芬胶囊中美沙芬与布洛芬含量

目的：建立复方美沙芬胶囊中美沙芬与布洛芬的高效液相色谱测定法。方法：固定相Ｓｈｉｍｐａｃｋ ＣＬＣ－ ＯＤＳ柱，流动相乙腈－ 水（６５∶３５，含０．００７ｍｏｌ·Ｌ－１ 硝酸铵、０．００７ｍｏｌ·Ｌ－ １ ＳＤＳ，用冰醋酸调ｐＨ至３．４），流速１．０ｍｌ·ｍｉｎ－１，在２７０ｎｍ 处测定。结果：美沙芬含量线性范围０ ．０２ ～０．１０ｍｇ·ｍｌ－１ ，回归方程：Ｙ＝２．５１×１０３ ＋１．８４×１０６Ｘ，ｒ ＝０．９９９６ ；布洛芬含量线性范围０ ．２０～１．００ｍｇ·ｍｌ－１ ，Ｙ＝ １．８６×１０３＋ ５．１６×１０５Ｘ，ｒ ＝０．９９９７ 。结论：含量测定方法简便快速，结果准确，可用于该制剂的质量控制。