For screening out salt tolerance germplasm and providing materials for ge- netic research of peanut, based on the indexes including relative germination poten- tial, relative germination rate, relative germination ind...For screening out salt tolerance germplasm and providing materials for ge- netic research of peanut, based on the indexes including relative germination poten- tial, relative germination rate, relative germination index and salt-injury rate, 128 peanut germplasms were selected for salt-tolerant identification and estimation under 2.5% NaCI. We found significant but various depressing levels of germination rate under salt stress among different germplasms, and only 5% of 128 germplasms were highly resistant to salinity. We also found that the relative germination index was a useful evaluation index for salt tolerance besides salt damage rate and rela- tive germination rate. After all, we comprehensively screened out 7 materials (JS011, JS024, JS125, JS491, JS523, JS524 and JS525) as salt tolerance germplasms for further research. Key展开更多
[Objective] The aim was to propose a new entropy weight fuzzy compre- hensive evaluation method for assessing cotton salt tolerance, realizing the objective, accurate and comprehensive evaluation of salt tolerance of ...[Objective] The aim was to propose a new entropy weight fuzzy compre- hensive evaluation method for assessing cotton salt tolerance, realizing the objective, accurate and comprehensive evaluation of salt tolerance of cotton. [Method] A sand culture experiment under salt stress of 150 mmol/L of NaCI was designed. The in- dicator weight was determined with the entropy weight fuzzy comprehensive evalu- ation method, based on the salt injury index of indicators. The salt tolerance of cotton was evaluated comprehensively. [Result] At the germination stage, the entropy and weight of salt injury index of germination energy, vigor index, hypocotyl length and fresh weight were highest, followed by germination rate and germination index, and of root length were lowest. At the seedling stage, the entropy and weight of salt injury index of plasma membrane permeability, root vigor and leaf expansion rate were highest, followed by plant height and net photosynthetic rate, and of shoot dry weight and root dry weight were lowest. The salt tolerance of cotton differed a- mong growth stages and cultivars. Among the 11 cultivars, CCRI-44 and CCRI-75 were steadily salt-tolerant at both germination and seedling stages; CCRI-17, Sumi- an 22, Sumian 15 and Dexiamianl had a stable moderate salt tolerance; while Sumian 12 and Simian 3 were steadily salt-sensitive. [Conclusion] The evaluated result was objective and exact, which indicated that this method could be used in comprehensive evaluation of salt tolerance of cotton.展开更多
The selection and application of salt tolerance varieties in peanut is impor- tant for the exploitation of saline soil. In this study, 41 peanut varieties were select- ed for the evaluation of salt tolerance in both g...The selection and application of salt tolerance varieties in peanut is impor- tant for the exploitation of saline soil. In this study, 41 peanut varieties were select- ed for the evaluation of salt tolerance in both germination and young seedling stage. The results showed the germination and growth of peanut were significantly inhibited by salt stress, which were enhanced by salt concentration increasing. The 0.5% NaCI was selected as the most suitable concentration for further identification, and the characters in germination stage (including the relative germination potential, relative germination rate and germination index) and young seedling stage (including relative main root length, seedling height, fresh weight, dry weight, et aL) were measured. Cluster analysis showed the varieties could be divided into different groups, and 9 and 3 salt-tolerant varieties were identified in the two stages, respec- tively, including two varieties performed well in both two stages, which could be useful for the breeding of new salt tolerance varieties and the study of salt toler- ance mechanism.展开更多
[Objectives] Salt stress is an important abiotic stress affecting soybean yield. This study aimed to screen salt-tolerant soybean varieties to improve the utilization efficiency of saline-alkali land. [Methods]Under t...[Objectives] Salt stress is an important abiotic stress affecting soybean yield. This study aimed to screen salt-tolerant soybean varieties to improve the utilization efficiency of saline-alkali land. [Methods]Under three concentrations of salt stress( 5,10 and 15 g/L),salt tolerance of nine Shandong soybean cultivars was identified at the germination stage. The number of germinated seeds,relative germination rate,plant height,radicle length and number of fibrous roots were recorded to analyze the relative salt damage index and evaluate the salt tolerance of different soybean cultivars. [Results]With the increase of salt concentration,the number of germinated seeds,plant height and number of fibrous roots showed a decreasing trend with significant differences among different soybean cultivars. Under three concentrations of salt stress,Shengdou 10,Lindou 10 and Hedou 28 exhibit relatively strong salt tolerance,indicating that these cultivars are relatively salt-tolerant and highly salt-tolerant cultivars; Hedou 12,Gaofeng 1,Weidou 9 and Qihuang 34 are moderately salt-tolerant cultivars; Qihuang 30 and Qihuang 31 are relatively sensitive to salt solution under 15 g/L NaC l stress. [Conclusions] This study provided high-quality germplasm materials for the improvement of salt tolerance of soybean varieties.展开更多
Soil salinity is one of the major environmental constraints that limits crop yield and nearly 7%of the total area worldwide is affected by salinity.Salinity-induced oxidative stress causes membrane damage during germi...Soil salinity is one of the major environmental constraints that limits crop yield and nearly 7%of the total area worldwide is affected by salinity.Salinity-induced oxidative stress causes membrane damage during germination and seedling growth.Indian mustard is a major oilseed crop in India and its production and productivity are severely affected by salt stress.Breeding Brassica cultivars for salinity tolerance by conventional means is very difficult and time-consuming.Therefore,understanding the molecular components associated with salt tolerance is needed to facilitate breeding for salt tolerance in Brassica.In this investigation,quantitative trait loci(QTLs)associated with salt tolerance were identified using F_(2:3)mapping population developed from a cross between CS52(salinity tolerant)and RH30(salinity sensitive).Parents and F_(2:3)were evaluated under controlled and salinity stress conditions for 14 morpho-physiological traits for two consecutive generations(F2 and F_(2:3)),explaining proportion of the phenotypic variance under control condition.Simple sequence repeat(SSR)markers were used for mapping studies.A genetic linkage map based on 42 simple sequence repeats(SSRs)markers was constructed covering 2298.5 cM(Haldane)to identify the loci associated with salt tolerance in Brassica juncea.Forty-one SSRs showing polymorphism in the parents(CS52 and RH30)were mapped on 8 linkage groups(C1–C8).One marker(nga 129)did not map to any of the linkage group and was excluded from mapping.Linkage group 5(C5;317.9 cM)was longest and linkage group 1(C1,255.0 cM)was shortest.Further,we identified 15 QTLs controlling 8 traits using F_(2:3)population.These QTLs explained 12.44–60.63%of the phenotypic variation with a LOD score range of 3.62–5.97.Out of these QTLs,QMI4.1 related to membrane injury showed 51.28%phenotypic variance with a LOD score of 3.34.QTL QBYP8.1 related to biological yield per plant showed 60.63%phenotypic variance at a LOD score of 3.62.The highest LOD score of 5.97 was recorded for QTL related to seed yield per plant(QSYP4.1).Major QTLs were QTL for biological yield per plant(QBYP8.1),QTL for siliquae per plant(QSP4.1),QTL for primary branches(QPB4.1),QTLs for seed per siliqua(QSS4.1,QSS4.2),QTL for seed yield per plant(QSYP4.1),and QTL for membrane injury(QMI8.1)which showed more than 50%phenotypic variance.These QTLs identified in our study need to be confirmed in other populations as well so that these can be used in marker-assisted selection and breeding to enhance salt tolerance in Brassica juncea.展开更多
土壤盐渍化是影响蚕豆生长的重要非生物胁迫之一。蚕豆耐盐碱性种质鉴定为挖掘耐盐碱基因和耐盐碱品种选育奠定基础,对利用盐碱地具有重要意义。本研究对155份国内外蚕豆种质全生育期利用基质+混合盐碱(9 g L^(-1) NaCl+Na_(2)CO_(3)+Na...土壤盐渍化是影响蚕豆生长的重要非生物胁迫之一。蚕豆耐盐碱性种质鉴定为挖掘耐盐碱基因和耐盐碱品种选育奠定基础,对利用盐碱地具有重要意义。本研究对155份国内外蚕豆种质全生育期利用基质+混合盐碱(9 g L^(-1) NaCl+Na_(2)CO_(3)+Na_(2)SO_(4),pH 10.5)进行胁迫处理。测定了成苗率、盐害指数、株高、鲜重、干重、叶绿素含量和氮含量7个指标,采用相关性、主成分、隶属函数和系统聚类分析,对各种质的耐盐碱性进行了综合评价和归类,并采用逐步回归分析建立了耐盐碱性预测回归方程。结果表明,(1)筛选出耐盐碱(20≤盐害指数<40)蚕豆种质3份,占1.94%;中耐盐碱(40≤盐害指数<60)种质8份,占5.16%;未发现高耐盐碱(盐害指数<20)蚕豆种质。(2)盐害指数与株高、鲜重、干重、叶绿素含量和氮含量呈极显著负相关(P<0.01)。(3)鲜重、株高、盐害指数、叶绿素含量和成苗率5个指标可以作为蚕豆全生育期耐盐碱鉴定评价指标。(4)155份蚕豆种质可分为2大类群,其中耐盐碱种质类群具有较高的成苗率、生物量、含氮量和叶绿素含量。研究结果可为蚕豆耐盐碱机理研究、耐盐碱基因挖掘和耐盐碱品种的选育提供可靠的材料。展开更多
基金Supported by National Peanut Industry Technology System"Nanjing Comprehensive Experimental Station"(CARS-14)the Fund of Independent Innovation of Agricultural Science and Technology in Jiangsu Province"On the Collection,Identification and Shared Service of Germplasm Resources of Main Field Crops"[CX(14)2001]~~
文摘For screening out salt tolerance germplasm and providing materials for ge- netic research of peanut, based on the indexes including relative germination poten- tial, relative germination rate, relative germination index and salt-injury rate, 128 peanut germplasms were selected for salt-tolerant identification and estimation under 2.5% NaCI. We found significant but various depressing levels of germination rate under salt stress among different germplasms, and only 5% of 128 germplasms were highly resistant to salinity. We also found that the relative germination index was a useful evaluation index for salt tolerance besides salt damage rate and rela- tive germination rate. After all, we comprehensively screened out 7 materials (JS011, JS024, JS125, JS491, JS523, JS524 and JS525) as salt tolerance germplasms for further research. Key
基金Supported by Jiangsu Agricultural Science and Technology Innovation Fund(CX(12)5035)Jiangsu Agricultural "Three New Engineering" Project(SXGC[2014]299)~~
文摘[Objective] The aim was to propose a new entropy weight fuzzy compre- hensive evaluation method for assessing cotton salt tolerance, realizing the objective, accurate and comprehensive evaluation of salt tolerance of cotton. [Method] A sand culture experiment under salt stress of 150 mmol/L of NaCI was designed. The in- dicator weight was determined with the entropy weight fuzzy comprehensive evalu- ation method, based on the salt injury index of indicators. The salt tolerance of cotton was evaluated comprehensively. [Result] At the germination stage, the entropy and weight of salt injury index of germination energy, vigor index, hypocotyl length and fresh weight were highest, followed by germination rate and germination index, and of root length were lowest. At the seedling stage, the entropy and weight of salt injury index of plasma membrane permeability, root vigor and leaf expansion rate were highest, followed by plant height and net photosynthetic rate, and of shoot dry weight and root dry weight were lowest. The salt tolerance of cotton differed a- mong growth stages and cultivars. Among the 11 cultivars, CCRI-44 and CCRI-75 were steadily salt-tolerant at both germination and seedling stages; CCRI-17, Sumi- an 22, Sumian 15 and Dexiamianl had a stable moderate salt tolerance; while Sumian 12 and Simian 3 were steadily salt-sensitive. [Conclusion] The evaluated result was objective and exact, which indicated that this method could be used in comprehensive evaluation of salt tolerance of cotton.
文摘The selection and application of salt tolerance varieties in peanut is impor- tant for the exploitation of saline soil. In this study, 41 peanut varieties were select- ed for the evaluation of salt tolerance in both germination and young seedling stage. The results showed the germination and growth of peanut were significantly inhibited by salt stress, which were enhanced by salt concentration increasing. The 0.5% NaCI was selected as the most suitable concentration for further identification, and the characters in germination stage (including the relative germination potential, relative germination rate and germination index) and young seedling stage (including relative main root length, seedling height, fresh weight, dry weight, et aL) were measured. Cluster analysis showed the varieties could be divided into different groups, and 9 and 3 salt-tolerant varieties were identified in the two stages, respec- tively, including two varieties performed well in both two stages, which could be useful for the breeding of new salt tolerance varieties and the study of salt toler- ance mechanism.
基金Supported by Agricultural Science and Technology Innovation Program of Shandong Academy of Agricultural Sciences(CXGC2016A02)Special Fund for Construction of Grain Industry Innovation Team of Modern Agricultural Industry Technology System of Shandong Province(SDARS-16-01)
文摘[Objectives] Salt stress is an important abiotic stress affecting soybean yield. This study aimed to screen salt-tolerant soybean varieties to improve the utilization efficiency of saline-alkali land. [Methods]Under three concentrations of salt stress( 5,10 and 15 g/L),salt tolerance of nine Shandong soybean cultivars was identified at the germination stage. The number of germinated seeds,relative germination rate,plant height,radicle length and number of fibrous roots were recorded to analyze the relative salt damage index and evaluate the salt tolerance of different soybean cultivars. [Results]With the increase of salt concentration,the number of germinated seeds,plant height and number of fibrous roots showed a decreasing trend with significant differences among different soybean cultivars. Under three concentrations of salt stress,Shengdou 10,Lindou 10 and Hedou 28 exhibit relatively strong salt tolerance,indicating that these cultivars are relatively salt-tolerant and highly salt-tolerant cultivars; Hedou 12,Gaofeng 1,Weidou 9 and Qihuang 34 are moderately salt-tolerant cultivars; Qihuang 30 and Qihuang 31 are relatively sensitive to salt solution under 15 g/L NaC l stress. [Conclusions] This study provided high-quality germplasm materials for the improvement of salt tolerance of soybean varieties.
文摘Soil salinity is one of the major environmental constraints that limits crop yield and nearly 7%of the total area worldwide is affected by salinity.Salinity-induced oxidative stress causes membrane damage during germination and seedling growth.Indian mustard is a major oilseed crop in India and its production and productivity are severely affected by salt stress.Breeding Brassica cultivars for salinity tolerance by conventional means is very difficult and time-consuming.Therefore,understanding the molecular components associated with salt tolerance is needed to facilitate breeding for salt tolerance in Brassica.In this investigation,quantitative trait loci(QTLs)associated with salt tolerance were identified using F_(2:3)mapping population developed from a cross between CS52(salinity tolerant)and RH30(salinity sensitive).Parents and F_(2:3)were evaluated under controlled and salinity stress conditions for 14 morpho-physiological traits for two consecutive generations(F2 and F_(2:3)),explaining proportion of the phenotypic variance under control condition.Simple sequence repeat(SSR)markers were used for mapping studies.A genetic linkage map based on 42 simple sequence repeats(SSRs)markers was constructed covering 2298.5 cM(Haldane)to identify the loci associated with salt tolerance in Brassica juncea.Forty-one SSRs showing polymorphism in the parents(CS52 and RH30)were mapped on 8 linkage groups(C1–C8).One marker(nga 129)did not map to any of the linkage group and was excluded from mapping.Linkage group 5(C5;317.9 cM)was longest and linkage group 1(C1,255.0 cM)was shortest.Further,we identified 15 QTLs controlling 8 traits using F_(2:3)population.These QTLs explained 12.44–60.63%of the phenotypic variation with a LOD score range of 3.62–5.97.Out of these QTLs,QMI4.1 related to membrane injury showed 51.28%phenotypic variance with a LOD score of 3.34.QTL QBYP8.1 related to biological yield per plant showed 60.63%phenotypic variance at a LOD score of 3.62.The highest LOD score of 5.97 was recorded for QTL related to seed yield per plant(QSYP4.1).Major QTLs were QTL for biological yield per plant(QBYP8.1),QTL for siliquae per plant(QSP4.1),QTL for primary branches(QPB4.1),QTLs for seed per siliqua(QSS4.1,QSS4.2),QTL for seed yield per plant(QSYP4.1),and QTL for membrane injury(QMI8.1)which showed more than 50%phenotypic variance.These QTLs identified in our study need to be confirmed in other populations as well so that these can be used in marker-assisted selection and breeding to enhance salt tolerance in Brassica juncea.
文摘土壤盐渍化是影响蚕豆生长的重要非生物胁迫之一。蚕豆耐盐碱性种质鉴定为挖掘耐盐碱基因和耐盐碱品种选育奠定基础,对利用盐碱地具有重要意义。本研究对155份国内外蚕豆种质全生育期利用基质+混合盐碱(9 g L^(-1) NaCl+Na_(2)CO_(3)+Na_(2)SO_(4),pH 10.5)进行胁迫处理。测定了成苗率、盐害指数、株高、鲜重、干重、叶绿素含量和氮含量7个指标,采用相关性、主成分、隶属函数和系统聚类分析,对各种质的耐盐碱性进行了综合评价和归类,并采用逐步回归分析建立了耐盐碱性预测回归方程。结果表明,(1)筛选出耐盐碱(20≤盐害指数<40)蚕豆种质3份,占1.94%;中耐盐碱(40≤盐害指数<60)种质8份,占5.16%;未发现高耐盐碱(盐害指数<20)蚕豆种质。(2)盐害指数与株高、鲜重、干重、叶绿素含量和氮含量呈极显著负相关(P<0.01)。(3)鲜重、株高、盐害指数、叶绿素含量和成苗率5个指标可以作为蚕豆全生育期耐盐碱鉴定评价指标。(4)155份蚕豆种质可分为2大类群,其中耐盐碱种质类群具有较高的成苗率、生物量、含氮量和叶绿素含量。研究结果可为蚕豆耐盐碱机理研究、耐盐碱基因挖掘和耐盐碱品种的选育提供可靠的材料。