为探究ICE(inducer of CBF expression)转录因子在高丛蓝莓花果发育进程中可能的调控机制,通过对VcICE基因家族进行全基因组鉴定及生物信息学分析,采用实时荧光定量PCR(qRT-PCR)技术分析VcICE基因亚家族成员在蓝莓花芽膨大和果实发育进...为探究ICE(inducer of CBF expression)转录因子在高丛蓝莓花果发育进程中可能的调控机制,通过对VcICE基因家族进行全基因组鉴定及生物信息学分析,采用实时荧光定量PCR(qRT-PCR)技术分析VcICE基因亚家族成员在蓝莓花芽膨大和果实发育进程中的相对表达水平.结果表明:在高丛蓝莓‘Draper’基因组中鉴定出8个VcICE成员,系统进化分析将其划分为2个亚家族,各亚家族成员均包含S-rich,bHLH和ACT等保守结构域;VcICE基因启动子区域富含光响应、植物激素、生长发育及非生物胁迫相关顺式作用元件;VcICE亚家族基因在高丛蓝莓‘O’Neal’和‘Bluerain’花芽膨大与果实发育进程中的相对表达差异显著.研究结果以期为深入了解VcICE转录因子在蓝莓花果发育进程中的功能提供理论参考.展开更多
An Antarctic sea ice identification algorithm on the HY-2A scatterometer（HSCAT） employs backscattering coefficient（σ0） and active polarization ratio（APR） for a preliminary sea ice identification.Then standard d...An Antarctic sea ice identification algorithm on the HY-2A scatterometer（HSCAT） employs backscattering coefficient（σ0） and active polarization ratio（APR） for a preliminary sea ice identification.Then standard deviation（STD） filtering and space filtering are carried out.Finally,it is used to identify sea ice.A process uses a σ0,STD threshold and an APR as sea ice indicators.The sea ice identification results are verified using the sea ice distribution data of the ASMR2 released by the National Snow and Ice Data Center as a reference.The results show very good consistence of sea ice development trends,seasonal changes,area distribution,and sea ice edge distribution of the sea ice identification results obtained by this algorithm relative to the ASMR2 sea ice results.The accuracy of a sea ice coverage is 90.8% versus the ASMR2 sea ice results.This indicates that this algorithm is reliable.展开更多
文摘为探究ICE(inducer of CBF expression)转录因子在高丛蓝莓花果发育进程中可能的调控机制,通过对VcICE基因家族进行全基因组鉴定及生物信息学分析,采用实时荧光定量PCR(qRT-PCR)技术分析VcICE基因亚家族成员在蓝莓花芽膨大和果实发育进程中的相对表达水平.结果表明:在高丛蓝莓‘Draper’基因组中鉴定出8个VcICE成员,系统进化分析将其划分为2个亚家族,各亚家族成员均包含S-rich,bHLH和ACT等保守结构域;VcICE基因启动子区域富含光响应、植物激素、生长发育及非生物胁迫相关顺式作用元件;VcICE亚家族基因在高丛蓝莓‘O’Neal’和‘Bluerain’花芽膨大与果实发育进程中的相对表达差异显著.研究结果以期为深入了解VcICE转录因子在蓝莓花果发育进程中的功能提供理论参考.
基金The National Science and Technology Support Program of China under contract No.2013BAD13B01the National Natural Science Foundation of China under contract No.41106152+3 种基金the National High Technology Research and Development Program(863Program)of China under contract No.2013AA09A505the International Science&Technology Cooperation Program of China under contract No.2011DFA22260the National High Technology Industrialization Project of China under contract No.[2012]2083the Marine Public Projects of China under contract Nos 201105032,201305032 and 201105002-07
文摘An Antarctic sea ice identification algorithm on the HY-2A scatterometer（HSCAT） employs backscattering coefficient（σ0） and active polarization ratio（APR） for a preliminary sea ice identification.Then standard deviation（STD） filtering and space filtering are carried out.Finally,it is used to identify sea ice.A process uses a σ0,STD threshold and an APR as sea ice indicators.The sea ice identification results are verified using the sea ice distribution data of the ASMR2 released by the National Snow and Ice Data Center as a reference.The results show very good consistence of sea ice development trends,seasonal changes,area distribution,and sea ice edge distribution of the sea ice identification results obtained by this algorithm relative to the ASMR2 sea ice results.The accuracy of a sea ice coverage is 90.8% versus the ASMR2 sea ice results.This indicates that this algorithm is reliable.
