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Isolation of extracellular CaM-binding proteins from transgenic tobacco seedlings with a magnetic method
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作者 GU Xuesong, CHEN Zhangliang and ZHU YuxianThe National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China 《Chinese Science Bulletin》 SCIE EI CAS 1997年第24期2081-2084,共4页
USING a magnetic method which was designed for identification of CaMBPs according to theprinciples of affinity chromatography, we were able to isolate 3 CaMBPs with calmodulin(CaM) binding activity from the cell wall ... USING a magnetic method which was designed for identification of CaMBPs according to theprinciples of affinity chromatography, we were able to isolate 3 CaMBPs with calmodulin(CaM) binding activity from the cell wall fractions of transgenic tobacco. After SDS-PAGEand CaM gel overlay analysis, we found that CaMBPs were retained and the isolation is effi- 展开更多
关键词 EXTRACELLULAR ISOLATION and identincation CaM-binding protein.
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