USING a magnetic method which was designed for identification of CaMBPs according to theprinciples of affinity chromatography, we were able to isolate 3 CaMBPs with calmodulin(CaM) binding activity from the cell wall ...USING a magnetic method which was designed for identification of CaMBPs according to theprinciples of affinity chromatography, we were able to isolate 3 CaMBPs with calmodulin(CaM) binding activity from the cell wall fractions of transgenic tobacco. After SDS-PAGEand CaM gel overlay analysis, we found that CaMBPs were retained and the isolation is effi-展开更多
文摘USING a magnetic method which was designed for identification of CaMBPs according to theprinciples of affinity chromatography, we were able to isolate 3 CaMBPs with calmodulin(CaM) binding activity from the cell wall fractions of transgenic tobacco. After SDS-PAGEand CaM gel overlay analysis, we found that CaMBPs were retained and the isolation is effi-