Optical microscopy is an essential tool for exploring the structures and activities of cells and tissues.To break the limit of resolution caused by diffraction,researchers have made continuous advances and innovations...Optical microscopy is an essential tool for exploring the structures and activities of cells and tissues.To break the limit of resolution caused by diffraction,researchers have made continuous advances and innovations to improve the resolution of optical microscopy since the 1990s.These contributions,however,still make sub-10nm imaging an obstacle.Here,we name a series of technologies as modulated illumination localization microscopy(MILM),which makes ultra-high-resolution imaging practical.Besides,we review the recent progress since 2017 when MINFLUX was proposed and became the inspiration and foundation for the follow-up devel-opment of MILM.This review divides MILM into two types:point-scanning and wide-field.The schematics,principles and future research directions of MILM are discussed elaborately.展开更多
Digital holographic microscopy(DHM),which combines digital holography with optical microscopy,is a wide field,minimally invasive quantitative phase microscopy(QPM)approach for measuring the 3D shape or the inner struc...Digital holographic microscopy(DHM),which combines digital holography with optical microscopy,is a wide field,minimally invasive quantitative phase microscopy(QPM)approach for measuring the 3D shape or the inner structure of transparent and translucent samples.However,limited by diffraction,the spatial resolution of conventional DHM is relatively low and incompatible with a wide field of view(FOV)owing to the spatial bandwidth product(SBP)limit of the imaging systems.During the past decades,many efforts have been made to enhance the spatial resolution of DHM while preserving a large FOV by trading with unused degrees of freedom.Illumination modulation techniques,such as oblique illumination,structured illumination,and speckle illumination,can enhance the resolution by adding more high-frequency information to the recording system.Resolution enhancement is also achieved by extrapolation of a hologram or by synthesizing a larger hologram by scanning the sample,the camera,or inserting a diffraction grating between the sample and the camera.For on-chip DHM,spatial resolution is achieved using pixel super-resolution techniques.In this paper,we review various resolution enhancement approaches in DHM and discuss the advantages and disadvantages of these approaches.It is our hope that this review will contribute to advancements in DHM and its practical applications in many fields.展开更多
基金This work was financially sponsored by National Natural Science Foundation of China(61735017,61827825)Major Program of the Natural Science Foundation of Zhejiang Province(LD21F050002)+1 种基金Key Research and Development Program of Zhejiang Province(2020C01116)Fundamental Research Funds for the Central Universities(K20200132),Zhejiang Lab(2020MC0AE01)and Zhejiang Provincial Ten Thousand Plan for Young Top Talents(2020R52001).Y.S.and L.Y.contributed equally to this work.
文摘Optical microscopy is an essential tool for exploring the structures and activities of cells and tissues.To break the limit of resolution caused by diffraction,researchers have made continuous advances and innovations to improve the resolution of optical microscopy since the 1990s.These contributions,however,still make sub-10nm imaging an obstacle.Here,we name a series of technologies as modulated illumination localization microscopy(MILM),which makes ultra-high-resolution imaging practical.Besides,we review the recent progress since 2017 when MINFLUX was proposed and became the inspiration and foundation for the follow-up devel-opment of MILM.This review divides MILM into two types:point-scanning and wide-field.The schematics,principles and future research directions of MILM are discussed elaborately.
基金the National Key Research and Development Program of China(2021YFF0700300)the National Natural Science Foundation of China(NSFC 62075177,62175112)+1 种基金the Natural Science Foundation of Shaanxi Province(2020JM-193 and 2020JQ-324)the Fundamental Research Funds for the Central Universities(XJS210503,XJS210504,JC2112,and JB210513).
文摘Digital holographic microscopy(DHM),which combines digital holography with optical microscopy,is a wide field,minimally invasive quantitative phase microscopy(QPM)approach for measuring the 3D shape or the inner structure of transparent and translucent samples.However,limited by diffraction,the spatial resolution of conventional DHM is relatively low and incompatible with a wide field of view(FOV)owing to the spatial bandwidth product(SBP)limit of the imaging systems.During the past decades,many efforts have been made to enhance the spatial resolution of DHM while preserving a large FOV by trading with unused degrees of freedom.Illumination modulation techniques,such as oblique illumination,structured illumination,and speckle illumination,can enhance the resolution by adding more high-frequency information to the recording system.Resolution enhancement is also achieved by extrapolation of a hologram or by synthesizing a larger hologram by scanning the sample,the camera,or inserting a diffraction grating between the sample and the camera.For on-chip DHM,spatial resolution is achieved using pixel super-resolution techniques.In this paper,we review various resolution enhancement approaches in DHM and discuss the advantages and disadvantages of these approaches.It is our hope that this review will contribute to advancements in DHM and its practical applications in many fields.
