The miniaturized femtosecond laser in near infrared-Ⅱregion is the core equipment of threephoton microscopy.In this paper,we design a compact and robust illumination source that emits dual-color linearly polarized li...The miniaturized femtosecond laser in near infrared-Ⅱregion is the core equipment of threephoton microscopy.In this paper,we design a compact and robust illumination source that emits dual-color linearly polarized light for three-photon microscopy.Based on an all-polarizationmaintaining passive mode-locked fiber laser,we shift the center wavelength of the pulses to the 1.7m band utilizing cascade Raman effect,thereby generate dual-wavelength pulses.To enhance clarity,the two wavelengths are separated through the graded-index multimode fiber.Then we obtain the dual-pulse sequences with 1639.4 nm and 1683.7 nm wavelengths,920 fs pulse duration,and 23.75 MHz pulse repetition rate.The average power of the signal is 53.64mW,corresponding to a single pulse energy of 2.25 nJ.This illumination source can be further amplified and compressed for three-photon fluorescence imaging,especially dual-color three-photon fluorescence imaging,making it an ideal option for biomedical applications.展开更多
To date,fluorescence imaging systems have all relied on at least one beam splitter(BS)to ensure the separation of excitation light and fluorescence.Here,we reported SiO2=TiO2 multi-layer long pass filter integrated Ga...To date,fluorescence imaging systems have all relied on at least one beam splitter(BS)to ensure the separation of excitation light and fluorescence.Here,we reported SiO2=TiO2 multi-layer long pass filter integrated GaN LED.It is considered as the potential source for imaging systems.Experimental results indicate that the GaN LED shows blue emission peaked at 470.3 nm and can be used to excite dye materials.Integrating with a long pass filter(550 nm),the light source can be used to establish a real-time fluorescence detection for dyes that emit light above 550 nm.More interestingly,with this source,a real-time imaging system with signature words written with the dyes,such as‘NJUPT’,can be converted into CCD images.This work may lead to a new strategy for integrating light sources and BS mirrors to build mini and smart fluorescence imaging systems.展开更多
基金supported by the Fundamental Re-search Funds for the Central Universities(HYGJXM202309).
文摘The miniaturized femtosecond laser in near infrared-Ⅱregion is the core equipment of threephoton microscopy.In this paper,we design a compact and robust illumination source that emits dual-color linearly polarized light for three-photon microscopy.Based on an all-polarizationmaintaining passive mode-locked fiber laser,we shift the center wavelength of the pulses to the 1.7m band utilizing cascade Raman effect,thereby generate dual-wavelength pulses.To enhance clarity,the two wavelengths are separated through the graded-index multimode fiber.Then we obtain the dual-pulse sequences with 1639.4 nm and 1683.7 nm wavelengths,920 fs pulse duration,and 23.75 MHz pulse repetition rate.The average power of the signal is 53.64mW,corresponding to a single pulse energy of 2.25 nJ.This illumination source can be further amplified and compressed for three-photon fluorescence imaging,especially dual-color three-photon fluorescence imaging,making it an ideal option for biomedical applications.
基金supported by the National Natural Science Foundation of China(Nos.62004103,61827804,and 62005130)the Natural Science Foundation of Jiangsu Province(Nos.BK20200755,BK20210593,and BK20200743)+4 种基金the“111”Project(No.D17018)the NUPTSF(No.NY220118)the STCSM(No.SKLSFO2020-04)the Foundation of Jiangsu Provincial Double-Innovation Doctor Program(No.30644)the Start-up Fund(Nos.NY219147 and NY220181).
文摘To date,fluorescence imaging systems have all relied on at least one beam splitter(BS)to ensure the separation of excitation light and fluorescence.Here,we reported SiO2=TiO2 multi-layer long pass filter integrated GaN LED.It is considered as the potential source for imaging systems.Experimental results indicate that the GaN LED shows blue emission peaked at 470.3 nm and can be used to excite dye materials.Integrating with a long pass filter(550 nm),the light source can be used to establish a real-time fluorescence detection for dyes that emit light above 550 nm.More interestingly,with this source,a real-time imaging system with signature words written with the dyes,such as‘NJUPT’,can be converted into CCD images.This work may lead to a new strategy for integrating light sources and BS mirrors to build mini and smart fluorescence imaging systems.
