The most common methods for three-dimensional reconstruction of peripheral nerve fascicles include histological and radiology techniques. Histological techniques have many drawbacks including an enormous manual worklo...The most common methods for three-dimensional reconstruction of peripheral nerve fascicles include histological and radiology techniques. Histological techniques have many drawbacks including an enormous manual workload and poor image registration. Micro-magnetic resonance imaging（Micro-MRI）, an emerging radiology technique, has been used to report results in the brain, liver and tumor tissues. However, micro-MRI usage for obtaining intraneural structures has not been reported. The aim of this study was to present a new imaging method for three-dimensional reconstruction of peripheral nerve fascicles by ~1T micro-MRI. Freshly harvested sciatic nerve samples from an amputated limb were divided into four groups. Two different scanning conditions（Mannerist Solution/GD-DTPA contrast agent, distilled water） were selected, and both T1 and T2 phases programmed for each scanning condition. Three clinical surgeons evaluated the quality of the images via a standardized scale. Moreover, to analyze deformation of the two-dimensional image, the nerve diameter and total area of the micro-MRI images were compared after hematoxylin-eosin staining. The results show that rapid micro-MRI imaging method can be used for three-dimensional reconstruction of the fascicle structure. Nerve sample immersed in contrast agent（Mannerist Solution/GD-DTPA） and scanned in the T1 phase was the best. Moreover, the nerve sample was scanned freshly and can be recycled for other procedures. MRI images show better stability and smaller deformation compared with histological images. In conclusion, micro-MRI provides a feasible and rapid method for three-dimensional reconstruction of peripheral nerve fascicles, which can clearly show the internal structure of the peripheral nerve.展开更多
A method based on the XYZLMS interim connection space is proposed to accurately acquire the multi-spectral images by digital still cameras. The XYZLMS values are firstly predicted from RGB values by polynomial model w...A method based on the XYZLMS interim connection space is proposed to accurately acquire the multi-spectral images by digital still cameras. The XYZLMS values are firstly predicted from RGB values by polynomial model with local training samples and then spectral reflectance is constructed from XYZLMS values by pseudo-inverse method. An experiment is implemented for multi-spectral image acquisition based on a commercial digital still camera. The results indicate that multi-spectral images can be accurately acquired except the very dark colors.展开更多
基金supported by grants from the National Key Research and Development Plan of China,No.31670986(to QTZ)the Science and Technology Project of Guangdong Province of China,No.2014B020227001,2017A050501017(to QTZ)the Science and Technology Project of Guangzhou of China,No.201807010082(to QTZ),201704030041(to JQ)
文摘The most common methods for three-dimensional reconstruction of peripheral nerve fascicles include histological and radiology techniques. Histological techniques have many drawbacks including an enormous manual workload and poor image registration. Micro-magnetic resonance imaging（Micro-MRI）, an emerging radiology technique, has been used to report results in the brain, liver and tumor tissues. However, micro-MRI usage for obtaining intraneural structures has not been reported. The aim of this study was to present a new imaging method for three-dimensional reconstruction of peripheral nerve fascicles by ~1T micro-MRI. Freshly harvested sciatic nerve samples from an amputated limb were divided into four groups. Two different scanning conditions（Mannerist Solution/GD-DTPA contrast agent, distilled water） were selected, and both T1 and T2 phases programmed for each scanning condition. Three clinical surgeons evaluated the quality of the images via a standardized scale. Moreover, to analyze deformation of the two-dimensional image, the nerve diameter and total area of the micro-MRI images were compared after hematoxylin-eosin staining. The results show that rapid micro-MRI imaging method can be used for three-dimensional reconstruction of the fascicle structure. Nerve sample immersed in contrast agent（Mannerist Solution/GD-DTPA） and scanned in the T1 phase was the best. Moreover, the nerve sample was scanned freshly and can be recycled for other procedures. MRI images show better stability and smaller deformation compared with histological images. In conclusion, micro-MRI provides a feasible and rapid method for three-dimensional reconstruction of peripheral nerve fascicles, which can clearly show the internal structure of the peripheral nerve.
基金supported by the National Natural Science Foundation of China(No.61205168)the National Science and Technology Support Program of China(No.2012BAH91F03)
文摘A method based on the XYZLMS interim connection space is proposed to accurately acquire the multi-spectral images by digital still cameras. The XYZLMS values are firstly predicted from RGB values by polynomial model with local training samples and then spectral reflectance is constructed from XYZLMS values by pseudo-inverse method. An experiment is implemented for multi-spectral image acquisition based on a commercial digital still camera. The results indicate that multi-spectral images can be accurately acquired except the very dark colors.
