Atherosclerosis and the role of immune cells

Atherosclerosis is a chronic inflammatory disease arising from lipids, specifically low-density lipoproteins, and leukocytes. Following the activation of endothelium with the expression of adhesion molecules and monocytes, inflammatory cytokines from macrophages, and plasmacytoid dendritic cells, high levels of interferon(IFN)-α and β are generated upon the activation of tolllike receptor-9, and T-cells, especially the ones with Th1 profile, produce pro-inflammatory mediators such as IFN-γ and upregulate macrophages to adhere to the endothelium and migrate into the intima. This review presents an exhaustive account for the role of immunecells in the atherosclerosis.

Epidermal growth factor receptor-targeted immune magnetic liposomes capture circulating colorectal tumor cells efficiently

AIM To compare the capacity of newly developed epidermal growth factor receptor(EGFR)-targeted immune magnetic liposomes(EILs) vs epithelial cell adhesion molecule(Ep CAM) immunomagnetic beads to capture colorectal circulating tumor cells(CTCs).METHODS EILs were prepared using a two-step method, and the magnetic and surface characteristics were confirmed. The efficiency of capturing colorectal CTCs as well as the specificity were compared between EILs and Ep CAM magnetic beads. RESULTS The obtained EILs had a lipid nanoparticle structure similar to cell membrane. Improved binding with cancer cells was seen in EILs compared with the method of coupling nano/microspheres with antibody. The binding increased as the contact time extended. Compared with Ep CAM immunomagnetic beads, EILs captured more CTCs in peripheral blood from colorectal cancer patients. The captured cells showed consistency with clinical diagnosis and pathology. Mutation analysis showed same results between captured CTCs and cancer tissues. CONCLUSION EGFR antibody-coated magnetic liposomes show high efficiency and specificity in capturing colorectal CTCs.

Review of copper and copper alloys as immune and antibacterial element

This review discussed the relationship among copper,human,and bacteria.Copper plays an important role in human immunity.Copper can boost human immune defense reactions at recommended intake level.The content mainly focused on copper antibacterial activity and copper antibacterial mechanisms.Conclusions stated that copper antibacterial activity is affected by copper homeostasis mechanisms in bacteria,adhesion,humidity,strain specificity,and manufacturing methods of antibacterial agents.For the preparation of particle antibacterial agents and surface antibacterial agents,this review discussed several manufacturing methods,such as sol−gel,cold spray,and biosynthesis belonging to chemical synthesis,physical synthesis,and biological synthesis,respectively.Sol−gel method contributes to the preparation of particle agents and surface agents.Cold spray technique is utilized in synthesis of surface copper agent.Biosynthesis is a novel technology which can be applied in nanoparticle agent preparation.

Circulating immune cell activation and diet: A review on human trials

Protein energy malnutrition is the main cause of immunodeficiency and, secondarily, of several infections. However, immune cell activation is involved in several pathophysiological processes that play a crucial role in the appearance of cardiovascular disease(CVD) or cancer. The aim of this review is to update the knowledge of the modulation of immune cell activation by different dietary patterns and its components focusing on CVD or cancer. While a westernized high-saturated fat highcarbohydrate diet is positively associated with lowgrade inflammation, vegetable- and fruit-based diets rich in monounsaturated fatty acids, polyunsaturated fatty acids and polyphenols, key nutrients of Mediterranean diet, decrease the levels of cellular and circulating inflammatory biomarkers thereby reducing the risk of related chronic diseases.

Correlation of body mass index with Th1/Th2 balance, adhesion molecules and insulin signal transduction in infertile patients

Objective: To study the correlation of body mass index with Th1/Th2 balance, adhesion molecules and insulin signal transduction in infertile patients. Methods: A total of 132 patients who received diagnostic curettage due to infertility in Tangshan Maternal and Child Health Hospital between June 2015 and March 2016 were selected as the research subjects and divided into the normal group with BMI<25 kg/m2, the overweight group with BMI 25-30 kg/m2 and the obesity group with BMI > 30 kg/m2 according to BMI, and the levels of Th1/Th2 cytokines in serum as well as the expression of Th1/Th2 transcription factors, adhesion molecules and insulin signal pathway molecules in endometrial tissue were detected. Results:IFN-γ and TNF-α levels in serum of obesity group and overweight group were significantly higher than those of control group while IL-4, IL-5 and IL-13 levels in serum as well as CD44V6, N-cadherin, FAK, ICAM-1, GLUT-4, IRS-1, PI3K and AKT mRNA expression in endometrial tissue were significantly lower than those of control group;IFN-γ and TNF-α levels in serum of obesity group were significantly higher than those of overweight group while IL-4, IL-5 and IL-13 levels in serum as well as CD44V6, N-cadherin, FAK, ICAM-1, GLUT-4, IRS-1, PI3K and AKT mRNA expression in endometrial tissue were significantly lower than those of overweight group. Conclusion: Weight gain can aggravate the Th1/Th2 disorder, reduce the adhesion molecule expression and hinder the insulin signal transduction in infertile patients.

重组贻贝粘蛋白联合他克莫司软膏与抗组胺药治疗面部糖皮质激素依赖性 皮炎的疗效及对皮肤屏障指标的影响

目的:研究重组贻贝粘蛋白联合他克莫司软膏、抗组胺药治疗面部糖皮质激素依赖性皮炎(简称面部皮炎)及对皮肤屏障指标的影响。方法:选取2020年4月-2022年12月笔者医院门诊面部皮炎患者131例,采用随机数字表法分为对照组(n=65)与研究组(n=66)。两组均对面部皮炎进行常规处理,对照组给予他克莫司与氯雷他定治疗,研究组在对照组基础上联合重组贻贝粘蛋白治疗,持续治疗4周后,比较两组皮肤屏障指标(表皮皮脂含量、角质层含水量,经表皮水分丢失量)、自觉症状与客观体征积分、疗效、不良反应。结果:治疗后两组表皮皮脂含量、角质层含水量上升,经表皮水分丢失量下降,且研究组表皮皮脂含量、角质层含水量高于对照组,经表皮水分丢失量低于对照组(P<0.05)。治疗4周后两组自觉症状与客观体征积分下降,且研究组积分低于对照组(P<0.05)。研究组总有效率93.94%高于对照组81.54%(P<0.05)。两组总不良反应发生率比较,差异无统计学意义(P>0.05)。结论:重组贻贝粘蛋白联合他克莫司软膏、抗组胺药能有效改善面部皮炎患者临床症状,促进恢复皮肤屏障功能,且无明显严重不良反应发生。

绵羊肺炎支原体致病机制研究进展

绵羊肺炎支原体是引起羊传染性胸膜肺炎的重要病原之一,是一种以咳嗽、喘气、渐进性消瘦以及肺间质增生性炎为特征的慢性呼吸道疾病,给羊养殖业造成了巨大的经济损失和动物生产力的下降。综合近期国内外关于绵羊肺炎支原体致病机制的文献报道,本文对Mo的黏附、免疫损伤机制进行了阐述,以期为我国绵羊支原体性肺炎的研究和防治提供参考。

人乳低聚糖与肠道菌群互作及其调节婴儿免疫功能的研究进展

人乳低聚糖(human milk oligosaccharides,HMOs)是母乳中的一类重要组分,迄今为止已经确定了200多种结构不同的HMOs。HMOs能在肠道微生物的作用下产生短链脂肪酸,对婴儿健康产生有益影响,这是母乳与配方粉的主要区别之一。研究表明,不同种类的肠道微生物利用不同机制来识别和消化不同结构的HMOs,维持肠道菌群平衡。同时,HMOs还具有缓解过敏症状,预防坏死性小肠结肠炎等多种功能。本文对HMOs的结构、种类与含量、与婴儿肠道菌群的互作及其免疫调节功能进行了综述。HMOs可促进婴儿肠道菌群的定植与生长,并通过多种方式直接或间接调节婴儿免疫功能。本文为HMOs在婴幼儿食品中的应用提供理论支持,对其未来研发方向提供新思路。

黏附性G蛋白偶联受体F1在胰腺导管腺癌中的表达及其促进癌症进展的机制研究

目的·分析黏附性G蛋白偶联受体F1(adhesion G protein-coupled receptor F1,ADGRF1)在胰腺导管腺癌(pancreatic ductal adenocarcinoma,PDAC)发生及发展过程中的表达变化,探究ADGRF1对PDAC细胞增殖的影响以及促进PDAC进展的潜在分子机制。方法·基于癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库和基因表达综合(Gene Expression Omnibus,GEO)数据库分析ADGRF1在正常胰腺组织及PDAC组织中的mRNA水平表达。利用实时荧光定量PCR(quantitative real-time PCR,qPCR)和蛋白质印迹法(Western blotting)检测ADGRF1在正常胰腺导管上皮细胞hTERT-HPNE及多种PDAC细胞中的表达情况。利用免疫组织化学染色(immunohistochemistry staining,IHC)检测PDAC患者的癌组织及癌旁组织中ADGRF1的表达差异。转染小干扰RNA(small interfering RNA,siRNA)敲低ADGRF1后,通过CCK8和平板克隆形成实验检测PDAC细胞AsPC-1、SW1990增殖能力的变化。构建稳定过表达ADGRF1的Patu8988细胞,通过CCK8实验检测过表达ADGRF1引起的PDAC细胞增殖变化。利用RNA测序(RNA-sequence,RNA-seq)、基因集富集分析(gene set enrichment analysis,GSEA)和免疫浸润分析预测与ADGRF1促进PDAC癌症进展相关的信号通路。结果·TCGA数据库和GEO数据库的分析结果显示ADGRF1 mRNA在PDAC组织中的表达高于正常胰腺组织(均P=0.000)。qPCR和Western blotting结果显示,与hTERT-HPNE细胞相比,多种PDAC细胞中ADGRF1的mRNA和蛋白水平均有所上调(均P<0.05)。IHC结果显示ADGRF1在PDAC患者癌组织中的表达也高于癌旁组织。此外,下调ADGRF1能够抑制PDAC细胞AsPC-1、SW1990的增殖能力;而过表达ADGRF1则促进Patu8988细胞的增殖能力(均P<0.05)。RNAseq、GSEA富集分析和免疫浸润的结果显示,ADGRF1的表达与干扰素α(interferon-α,IFN-α)、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)和核因子κB(nuclear factorκB,NF-κB)等信号通路有关。结论·ADGRF1在PDAC细胞和组织中高表达,促进PDAC细胞的增殖,其机制可能与多个免疫相关信号通路有关。

Role of adhesion molecules in cancer and targeted therapy

Adhesion molecules mediate cell-to-cell and cell-to-extracellular matrix interactions and transmit mechanical and chemical signals among them.Various mechanisms deregulate adhesion molecules in cancer,enabling tumor cells to proliferate without restraint,invade through tissue boundaries,escape from immune surveillance,and survive in the tumor microenvironment.Recent studies have revealed that adhesion molecules also drive angiogenesis,reshape metabolism,and are involved in stem cell self-renewal.In this review,we summarize the functions and mechanisms of adhesion molecules in cancer and the tumor microenvironment,as well as the therapeutic strategies targeting adhesion molecules.These studies have implications for furthering our understanding of adhesion molecules in cancer and providing a paradigm for exploring novel therapeutic approaches.

槐杞黄颗粒联合西药治疗小儿紫癜性肾炎临床研究

目的:观察槐杞黄颗粒联合西药治疗小儿紫癜性肾炎的临床疗效。方法:纳入74例气阴两虚型紫癜性肾炎患儿,采用随机数字表法分成治疗组和对照组各37例。2组均给予醋酸泼尼松龙片、硫唑嘌呤片治疗,治疗组联合槐杞黄颗粒治疗,连续治疗3个月。治疗前及治疗3个月后检测尿沉渣红细胞计数、24 h尿蛋白定量、免疫功能指标[CD3^(+)、CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+)比值]、纤维蛋白原(FIB)及血管细胞黏附分子-1(VCAM-1)。观察治疗期间2组患儿的不良反应发生情况。比较2组的临床疗效。结果:治疗3个月,治疗组临床疗效总有效率高于对照组(P<0.05)。2组CD3^(+)、CD4^(+)水平及CD4^(+)/CD8^(+)比值与治疗前相比均升高(P<0.05),CD8^(+)水平与治疗前相比均下降(P<0.05);治疗组CD3^(+)、CD4^(+)水平及CD4^(+)/CD8^(+)比值均高于对照组(P<0.05),CD8^(+)水平低于对照组(P<0.05)。2组尿沉渣红细胞计数、24 h尿蛋白定量及FIB、VCAM-1水平与治疗前相比均降低(P<0.05),治疗组尿沉渣红细胞计数、24 h尿蛋白定量及FIB、VCAM-1水平均低于对照组(P<0.05)。用药期间2组患儿均未见明显不良反应。结论:槐杞黄颗粒联合醋酸泼尼松龙片、硫唑嘌呤片治疗气阴两虚型小儿紫癜性肾炎临床疗效确切,可显著提高患儿的免疫力,减轻炎症损伤,改善体内血液高凝状态,进一步保护肾脏,且未见明显不良反应。

Recent progress in application of nanovaccines for enhancing mucosal immune responses

Mucosal vaccines that stimulate both mucosal and systemic immune responses are desirable,as they could prevent the invading pathogens at their initial infection sites in a convenient and userfriendly way. Nanovaccines are receiving increasing attention for mucosal vaccination due to their merits in overcoming mucosal immune barriers and in enhancing immunogenicity of the encapsulated antigens.Herein, we summarized several nanovaccine strategies that have been reported for enhancing mucosal immune responses, including designing nanovaccines that have superior mucoadhesion and mucus penetration capacity, designing nanovaccines with better targeting efficiency to M cells or antigen-presenting cells, and co-delivering adjuvants by using nanovaccines. The reported applications of mucosal nanovaccines were also briefly discussed, including prevention of infectious diseases, and treatment of tumors and autoimmune diseases. Future research progresses in mucosal nanovaccines may promote the clinical translation and application of mucosal vaccines.

PAMs免疫粘附受体CR 1-like对PRRSV感染的影响

[目的]本试验旨在探讨猪肺泡巨噬细胞(Porcine alveolar macrophages,PAMs)表面天然免疫粘附受体—猪I型补体受体(Porcine complement receptor 1⁃like,CR1⁃like)对猪繁殖与呼吸综合征病毒(Porcine reproductive and respi⁃ratory syndrome virus,PRRSV)感染PAMs的影响,为阐述CR1⁃like在PRRSV免疫应答中的生物学功能提供理论数据。[方法]采取PRRSV减毒活疫苗免疫接种PRRSV抗体为阴性的长白仔猪20~28 d后,提取分离抗病毒血清并检测抗PRRSV血清中和效价;将抗PRRSV血清进行不同倍比稀释,制备各类PRRSV抗原抗体复合物并接种于PAMs上,建立抗体依赖性增强(Antibody⁃dependent enhancement,ADE)模型,qRT⁃PCR技术检测各时间点PRRSV N基因拷贝数变化;PRRSV感染PAMs 9 h后,将抗血清和猪新鲜血清共处理细胞,运用ELISA测定不同时间点细胞上清液中猪新鲜血清C3b、C4b、CH50总活性;将PAMs分为试验组(PRRSV感染PAMs 9 h后同时加入抗血清和猪新鲜血清)和6个对照组,采用qRT⁃PCR技术检测各组不同时间点胞内和上清中PRRSV N基因的拷贝数,确定猪新鲜血清对PRRSV感染PAMs的作用;采用免疫阻断技术将PAMs分为FcR McAb阻断组、CR1⁃like McAb阻断组、共阻断组及3个对照组,qRT⁃PCR技术检测PRRSV N基因拷贝数变化,确定免疫粘附受体CR1⁃like对PRRSV感染PAMs的影响。[结果]抗PRRSV血清在PAMs上的中和抗体效价为1∶5.37;qRT⁃PCR结果成功筛选出1:20稀释度的抗PRRSV血清对病毒的复制具有促进作用;ELISA检测猪新鲜血清C3b、C4b和CH50浓度随PRRSV感染时间延长呈降低趋势;qRT⁃PCR结果显示,猪新鲜血清可以与1∶20稀释度抗PRRSV血清协同促进PRRSV的复制;qRT⁃PCR、免疫阻断技术得出1∶20稀释度抗PRRSV血清、猪新鲜血清协同促进PRRSV感染增强不只依赖于FcR,还与CR1⁃like有关。[结论]体外条件下,PAMs表面的CR1⁃like是介导PRRSV感染PAMs过程中产生ADE效应的分子之一,可与FcR协同促进PRRSV感染PAMs。

FOXL2NB在结直肠癌中的表达和临床意义

目的分析叉头框L2旁基因(FOXL2NB)在结直肠癌(CRC)中的表达及临床意义,探讨FOXL2NB对CRC细胞的作用。方法RT-qPCR和蛋白免疫印迹分析CRC组织和细胞中FOXL2NB的表达;Transwell和CCK-8实验探讨FOXL2NB在SW620细胞增殖和迁移的作用;Kaplan-Meier法和多因素Cox回归分析评估FOXL2NB对CRC预后的影响;基因集富集分析比较高、低风险组间分子信号通路差异,CIBERSORT R包计算CRC组织中FOXL2NB表达与免疫细胞浸润的关系,limma R包计算与FOXL2NB表达显著相关的分子。结果FOXL2NB在CRC组织和细胞中的表达水平显著升高(P<0.05);敲低FOXL2NB可抑制CRC细胞的迁移及增殖;高水平的FOXL2NB与CRC患者不良预后相关(P<0.05),可作为CRC预后的独立风险因素(HR=2.27,95%CI=1.78~2.9,P<0.001);FOXL2NB高表达的CRC中细胞黏附分子和肿瘤免疫相关通路被显著富集,CD8+T细胞和活化树突状细胞显著增多,M0巨噬细胞显著减少。FOXL2NB基因表达与SCRT2、MGAT5B、SELENOV、FOXB1、KCNA4、LRRC14B和SLC32A1基因表达正相关。结论FOXL2NB在CRC中高表达,可能预示CRC患者不良预后。FOXL2NB可能促进结直肠癌细胞增殖和迁移。

免疫浸润相关基因mRNA在宫颈癌与宫颈癌前病变中的表达及其与预后的关系

目的探讨免疫浸润相关基因mRNA在宫颈癌与宫颈癌前病变中的表达水平及其与预后的关系。方法选取延安大学附属医院2017年1月至2019年1月宫颈癌患者74例作为宫颈癌变组,宫颈癌前病变患者74例作为宫颈癌前病变组,另选取同期因子宫肌瘤行子宫切除术患者74例作为宫颈正常组,比较3组免疫浸润相关基因[人类白细胞抗原G(HLA-G)、叉状头螺旋转录因子3(FOXP3)、T细胞免疫球蛋白粘连蛋白-3(TIM-3)]、恶性生物学行为相关基因[侵袭基因p21-活化的激酶6(PAK-6)、zeste 2多梳抑制复合物2亚基增强子(EZH2)、增殖基因血管生成素样蛋白4(ANGPTL4)、同源盒基因B7(HOXB7)]的mRNA相对表达量,分析免疫浸润相关基因与宫颈癌发生及宫颈癌恶性生物学行为的关系,并统计对比不同免疫浸润相关基因表达水平患者3年生存率。结果HLA-G、FOXP3、TIM-3 mRNA相对表达量为宫颈癌变组>宫颈癌前病变组>宫颈正常组(P<0.05);不同临床分期、分化程度,以及有无淋巴结转移、有无脉管浸润患者的HLA-G、FOXP3、TIM-3、PAK-6、EZH2、ANGPTL4、HOXB7 mRNA相对表达量差异有统计学意义(P<0.05);HLA-G、FOXP3、TIM-3的mRNA高表达患者宫颈癌前病变进展至宫颈癌的风险分别是低表达患者的3.005倍、4.654倍、3.343倍;宫颈癌患者HLA-G、FOXP3、TIM-3 mRNA与侵袭相关基因PAK-6、EZH2 mRNA、增殖相关基因ANGPTL4、HOXB7的mRNA表达呈正相关(P<0.05);随访3年,失访2例,72例宫颈癌患者3年生存率为68.06%(49/72),HLA-G、FOXP3、TIM-3的mRNA高表达患者3年生存率均低于低表达患者(P<0.05)。结论免疫浸润相关基因HLA-G、FOXP3、TIM-3的mRNA在宫颈癌与宫颈癌前病变中呈上调表达,会显著增加宫颈癌发病风险,且与宫颈癌恶性生物学行为密切相关,还可能对生存预后产生重要影响。

嗜热链球菌S131对巨噬细胞的免疫调节作用研究

该实验旨在研究嗜热链球菌(Streptococcus thermophilus)S131调节免疫力的功能,并对其提升免疫力的作用机制进行解析。首先,对菌株定殖肠道和产胞外多糖的能力进行评价;然后,通过细胞实验检测菌株对巨噬细胞RAW264.7(小鼠单核巨噬细胞白血病细胞)的增殖、吞噬及对其分泌细胞因子的影响。结果表明,嗜热链球菌S131具有较好的黏附能力,可以高产胞外多糖。其处理RAW264.7细胞后其相对增殖率和吞噬率分别为131.11%和115.13%,且可促细胞分泌肿瘤坏死因子(tumor necrosis factor,TNF-α)、白细胞介素-10(interleukin-10,IL-10)和IL-12细胞因子。综上所述,嗜热链球菌S131可黏附于肠道,可高产胞外多糖,同时可激活巨噬细胞,引起生理性炎症反应,提高机体免疫,能够用于提升免疫力功能食品的开发。

SKAP1通过调控ICAM-1对宫颈癌模型小鼠肿瘤生长、转移及免疫调节机制研究

目的 探讨SKAP1通过调控细胞间黏附分子-1(ICAM-1)对宫颈癌模型小鼠肿瘤生长、转移及免疫调节的机制。方法 培养人宫颈癌细胞He La,构建SKAP1-mi RNA慢病毒干扰质粒和阴性对照Neg-mi RNA空白慢病毒干扰质粒,分别感染He La细胞,随机分为对照组(细胞未做处理)、空质粒组(Neg-mi RNA组)和SKAP1基因沉默组(SKAP1-mi RNA组),每组10只。将稳定表达的3组细胞配制成单细胞悬液分别接种于对应组BALB/c裸鼠右前腋窝中部外侧皮下,记录各组肿瘤生长情况,30 d后采用活体荧光成像技术检测肿瘤转移情况。处死裸鼠,取肿瘤组织,采用RealTime PCR法检测各组裸鼠肿瘤组织SKAP1、ICAM-1 m RNA相对表达量,免疫组化法检测SKAP1、ICAM-1蛋白表达情况,Western blot法检测SKAP1、ICAM蛋白相对表达量,流式细胞术检测免疫细胞CD3+、CD4+、CD8+水平,ELISA法检测Th1和Th2细胞因子水平。结果 细胞接种后饲养期间内,对照组和空质粒组裸鼠各死亡1只,SKAP1基因沉默组死亡2只。各组瘤体体积均随时间延长而增大(P<0.05);与对照组、空质粒组比较,SKAP1基因沉默组各时间段瘤体体积减小、肿瘤细胞转移面积减小、荧光信号强度降低、肿瘤组织SKAP1和ICAM-1 m RNA及其蛋白相对表达量均降低,差异有统计学意义(P<0.05);与对照组、空质粒组比较,SKAP1基因沉默组肿瘤组织免疫细胞CD3+、CD4+、CD4+/CD8+及Th1细胞因子IL-2、IFN-γ水平均升高,CD8+及Th2细胞因子IL-4、IL-10水平均降低(P<0.05)。结论 沉默SKAP1基因可以抑制He La细胞宫颈癌裸鼠移植瘤的生长及转移,逆转小鼠Th1向Th2漂移,恢复Th1表达优势,这一作用可能是通过下调ICAM-1表达实现的。

淋巴细胞功能相关抗原1/细胞间黏附分子在眼干燥症中的发病机制及免疫抑制相关药物的研究进展

淋巴细胞功能相关抗原1(LFA-1)与细胞间黏附分子(ICAM-1)是同源配体,LFA-1/ICAM-1的相互作用是免疫细胞增殖和浸润的关键。眼干燥症的发病有传入臂、传出臂两条通路。传入臂即抗原提呈细胞受眼表刺激后转移至淋巴结;传出臂即激活的淋巴细胞从淋巴结转移至眼表。LFA-1/ICAM-1在传入臂与传出臂上都发挥重要作用,对免疫稳态破坏相关疾病的诊断和治疗有重要意义。从免疫病理角度治疗眼干燥症的药物Lifitegrast、皮质类固醇与环孢素A在临床已见成效。本文就LFA-1/ICAM-1在眼干燥症中的发病机制及免疫抑制相关药物的研究进展做一综述。

血清sICAM-1和TGF-β1水平、RBC-ICR率及淋巴细胞比例与剖宫产术后发生产褥感染的关系

目的 分析血清可溶性细胞间黏附分子1(sICAM-1)和转化生长因子β1(TGF-β1)水平、红细胞免疫复合物花环(RBC-ICR)率、淋巴细胞比例与剖宫产术后发生产褥感染的关系。方法 选取108例剖宫产术后发生产褥感染的产妇作为研究组,依据感染程度将其分为轻度组(41例)、中度组(35例)与重度组(32例)。另选取剖宫产术后未发生产褥感染的60例产妇作为对照组。比较研究组与对照组之间,以及轻度组、中度组与重度组之间产前血清sICAM-1和TGF-β1水平、 RBC-ICR率、淋巴细胞比例。采用多因素Logistic回归模型分析影响剖宫产术后发生产褥感染的因素。结果 研究组的产前血清sICAM-1和TGF-β1水平、 RBC-ICR率、淋巴细胞比例高于对照组(P<0.05)。轻度组、中度组、重度组的产前血清sICAM-1和TGF-β1水平、 RBC-ICR率及淋巴细胞比例依次升高(P<0.05)。多因素Logistic回归分析结果显示,校正混杂因素后,产前血清TGF-β1水平、RBC-ICR率、淋巴细胞比例是剖宫产术后发生产褥感染的独立影响因素(P<0.05)。结论 剖宫产术后发生产褥感染的产妇产前血清sICAM-1和TGF-β1水平、 RBC-ICR率、淋巴细胞比例升高,且四项指标均随着感染程度的加重而升高。产前血清TGF-β1水平、RBC-ICR率、淋巴细胞比例升高的产妇,其剖宫产术后发生产褥感染的风险增加。

2种仙人掌多糖对S180小鼠红细胞免疫功能影响的研究

目的:研究仙人掌多糖对S180小鼠红细胞免疫功能的影响。方法:应用药理方法及化学试剂盒进行测定。结果:仙人掌多糖可增加荷瘤小鼠肿瘤红细胞花环率,提高红细胞C3b受体花环促进率,降低红细胞C3b受体花环抑制率,提高红细胞表面唾液酸含量。药用仙人掌多糖中剂量组及食用仙人掌多糖高剂量组效果显著(P<0.01)。结论:仙人掌多糖可以改善荷瘤小鼠红细胞免疫功能,可能是其抗肿瘤作用的机制之一。