Immune Clone Maximum Likelihood Estimation of Improved Non-homogeneous Poisson Process Model Parameters

Aiming at the solving problem of improved nonhomogeneous Poisson process( NHPP) model in engineering application,the immune clone maximum likelihood estimation( MLE)method for solving model parameters was proposed. The minimum negative log-likelihood function was used as the objective function to optimize instead of using iterative method to solve complex system of equations,and the problem of parameter estimation of improved NHPP model was solved by immune clone algorithm. And the interval estimation of reliability indices was given by using fisher information matrix method and delta method. An example of failure truncated data from multiple numerical control( NC) machine tools was taken to prove the method. and the results show that the algorithm has a higher convergence rate and computational accuracy, which demonstrates the feasibility of the method.

An Improved Immune Clone Selection Algorithm for Parameters Optimization of Marine Electric Power System Stabilizer

In themarine electric power system,the marine generators will be disturbed by the large change of loads or the fault of the power system.The marine generators usually installed power system stabilizers to damp power system oscillations through the excitation control.This paper proposes a novel method to obtain optimal parameter values for Power System Stabilizer(PSS)to suppress low-frequency oscillations in the marine electric power system.In this paper,a newly developed immune clone selection algorithm was improved from the three aspects of the adaptive incentive degree,vaccination,and adaptive mutation strategies.Firstly,the typical PSS implementation type of leader-lag structure was adopted and the objective function was set in the optimization process.The performance of PSS tuned by improved immune clone selection algorithm was compared with PSS tuned by basic immune clone selection algorithm(ICSA)under various operating conditions and disturbances.Then,an improved immune clone selection algorithm(IICSA)optimization technique was implemented on two test systems for test purposes.Based on the simulations,it is found that an improved immune clone selection algorithm demonstrates superiority over the basic immune clone selection algorithm in getting a smaller number of iterations and fast convergence rates to achieve the optimal parameters of the power system stabilizers.Moreover,the proposed approach improves the stability and dynamic performance under various loads conditions and disturbances of the marine electric power system.

Improved algorithms to plan missions for agile earth observation satellites

This study concentrates of the new generation of the agile （AEOS）. AEOS is a key study object on management problems earth observation satellite in many countries because of its many advantages over non-agile satellites. Hence, the mission planning and scheduling of AEOS is a popular research problem. This research investigates AEOS characteristics and establishes a mission planning model based on the working principle and constraints of AEOS as per analysis. To solve the scheduling issue of AEOS, several improved algorithms are developed. Simulation results suggest that these algorithms are effective.

Construction of a recombinant plasmid harbouring the rhoptry protein 1 gene of Toxoplasma gondii and preliminary observations on DNA immunity

Objective To observe the immune responses elicited in BALB/c mice by a DNA vaccine. A gene encoding rhoptry protein 1 (ROP1) from Toxoplasma gondii (T. gondii) was cloned into vector pcDNA3. Methods Amplifyied gene fragments coding for ROP1 from the genomic DNA of T.gondii ZS2 were inserted into cloning vector, pUC18, and sub-cloned into pcDNA3. Mice were injected at a dosage of 100?μg recombinant plasmid DNA by intramuscular injection and boosted after 2 weeks. pcDNA3 and normal saline were used as control. 30, 50 and 70 days after the second immunization, NK cell activity, T lymphocyte proliferation and sub-clusters and serum IgG antibody were assayed.Results The specific gene fragment coding for ROP1 was amplified and a pcROP1 recombinant was constructed. At 30 days after immunization, the spleens of the mice were obviously enlarged evidently. NKC activity and the proliferation of spleen T lymphocytes seen on MTT assay were higher in pcROP1 group than in the controls. The number of CD4+ T cells exhibited no obvious increase compared with that of the control, but CD8+ T cells were obviously increased (P<0.05). At 90 days after vaccination, the titer of IgG antibody in the serum of vaccinated mice was positive (1∶100). Conclusion pcROP1 was constructed and it could elicit both cellular and humoral immune responses in immunized mice.