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A New Voltammetric Enzyme Immunoassay System for the Detection of Alkaline Phosphatase
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作者 Kui JIAO Wei SUN Hai Yu WANG 《Chinese Chemical Letters》 SCIE CAS CSCD 2002年第1期69-70,共2页
A new voltammetric enzyme immunoassay system was investigated based on p-nitrophenyl phosphate (PNPP) as the substrate for alkaline phosphatase (ALP). PNPP is enzymatically hydrolyzed and the product p-nitrophenol (P... A new voltammetric enzyme immunoassay system was investigated based on p-nitrophenyl phosphate (PNPP) as the substrate for alkaline phosphatase (ALP). PNPP is enzymatically hydrolyzed and the product p-nitrophenol (PNP) is detected by differential pulse voltammetry (DPV), which can be oxidized at +1.02 V (vs. Ag/AgCl) on bare glass carbon electrode (GCE). The conditions for enzymatic reaction and electrochemical detection were studied. According to this method, ALP can be detected with a detection limit of 2.8102 mU/L and a linear range of 4.0102 ~ 1.0106 mU/L. 展开更多
关键词 Alkaline phosphatase p-nitrophenyl phosphate P-NITROPHENOL differential pulse voltammetry voltammetric enzyme immunoassay.
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On-site rapid detection of multiple pesticide residues in tea leaves by lateral flow immunoassay
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作者 Junxia Gao Tianyi Zhang +7 位作者 Yihua Fang Ying Zhao Mei Yang Li Zhao Ye Li Jun Huang Guonian Zhu Yirong Guo 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2024年第2期276-283,共8页
The application of pesticides (mostly insecticides and fungicides) during the tea-planting process will undoubtedly increase the dietary risk associated with drinking tea. Thus, it is necessary to ascertain whether pe... The application of pesticides (mostly insecticides and fungicides) during the tea-planting process will undoubtedly increase the dietary risk associated with drinking tea. Thus, it is necessary to ascertain whether pesticide residues in tea products exceed the maximum residue limits. However, the complex matrices present in tea samples comprise a major challenge in the analytical detection of pesticide residues. In this study, nine types of lateral flow immunochromatographic strips (LFICSs) were developed to detect the pesticides of interest (fenpropathrin, chlorpyrifos, imidacloprid, thiamethoxam, acetamiprid, carbendazim, chlorothalonil, pyraclostrobin, and iprodione). To reduce the interference of tea substrates on the assay sensitivity, the pretreatment conditions for tea samples, including the extraction solvent, extraction time, and purification agent, were optimized for the simultaneous detection of these pesticides. The entire testing procedure (including pretreatment and detection) could be completed within 30 min. The detected results of authentic tea samples were confirmed by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), which suggest that the LFICS coupled with sample rapid pretreatment can be used for on-site rapid screening of the target pesticide in tea products prior to their market release. 展开更多
关键词 Lateral flow immunoassay Rapid detection Pesticide multi-residue Tea matrix Sample rapid pretreatment
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Enzyme-catalyzed reaction of OPD-H_2O_2-HRP voltammetric enzyme-linked immunoassay system 被引量:3
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作者 焦奎 孙刚 张书圣 《Science China Chemistry》 SCIE EI CAS 1998年第4期345-352,共8页
Enzyme catalyzed reaction of o phenylenediamine (OPD) H\-2O\-2 horseradish peroxidase (HRP) voltammetric enzyme linked immunoassay system has been studied in detail with electrochemical analysis, high performance liqu... Enzyme catalyzed reaction of o phenylenediamine (OPD) H\-2O\-2 horseradish peroxidase (HRP) voltammetric enzyme linked immunoassay system has been studied in detail with electrochemical analysis, high performance liquid chromatography (HPLC), ultraviolet/visible (UV/Vis) spectroscopy, infrared (IR) spectroscopy and nuclear magnetic resonance (NMR) spectroscopy. The pure product of H\-2O\-2 oxidizing OPD catalyzed by HRP was prepared with chemical method. The experimental results of voltammetry and HPLC indicate that only one product of enzyme catalyzed reaction has been obtained under the selected enzyme catalyzed reaction conditions. Identifications by UV/Vis spectrum, IR spectrum and 13 C NMR spectrum show that the product is 2,3 diaminophenazine. The processes of the enzyme catalyzed reaction and the electroreduction of the product of the enzyme catalyzed reaction are described. 展开更多
关键词 o PHENYLENEDIAMINE VOLTAMMETRY immunoassay enzyme catalyzing HORSERADISH peroxidase.
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Enzyme-catalyzed reaction of voltammetric enzyme-linked immunoassay system based on OAP as substrate
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作者 张书圣 陈洪渊 焦奎 《Science China Chemistry》 SCIE EI CAS 1999年第2期195-203,共9页
Theo-aminophenol (OAP)-H2O2-horseradish peroxidase (HRP) voltammetric enzyme-linked immunoassay new system has extremely high sensitivity. HRP can be measured with a detection limit of 6.0×1010 g/L and a linear r... Theo-aminophenol (OAP)-H2O2-horseradish peroxidase (HRP) voltammetric enzyme-linked immunoassay new system has extremely high sensitivity. HRP can be measured with a detection limit of 6.0×1010 g/L and a linear range of 1.0×10-9-4.0×10-6 g/L. The pure product of H2O2 oxidizing OAP catalyzed by HRP was prepared with chemical method. The enzyme-catalyzed reaction has been investigated with electroanalytical chemistry, UV/Vis spectrum, IR spectrum,13C NMR,1H NMR, mass spectrum, elemental analysis, etc. Under the selected enzyme-catalyzed reaction conditions, the oxidation product of OAP with H2O2 catalyzed by HRP is 2-aminophenoxazine-3-one. The processes of the enzyme-catalyzed reaction and the electroreduction of the product of the enzymecatalyzed reaction have been described. 展开更多
关键词 O-AMINOPHENOL HORSERADISH PEROXIDASE VOLTAMMETRY enzyme-catalysis electrochemical immunoassay.
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Harmonization of SARS-CoV-2 antigen immunoassays:are they measuring the same“thing”?
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作者 Giuseppe Lippi Gian Luca Salvagno +1 位作者 Gianluca Gianfilippi Brandon Michael Henry 《Infectious Diseases Research》 2023年第1期5-9,共5页
Background:This study was planned to assess the accuracy and comparability of two commercially available,laboratory-based SARS-CoV-2(severe acute respiratory syndrome)antigen(Ag)immunoassays.Methods:We studied a cohor... Background:This study was planned to assess the accuracy and comparability of two commercially available,laboratory-based SARS-CoV-2(severe acute respiratory syndrome)antigen(Ag)immunoassays.Methods:We studied a cohort of subjects with acute SARS-CoV-2 infection,from whom a nasopharyngeal swab was taken and tested with a molecular assay(Altona Diagnostics RealStar SARS-CoV-2 RT-PCR Kit)and two laboratory-based,fully automated SARS-CoV-2 Ag immunoassays(Fujirebio Lumipulse G SARS-CoV-2 Ag and Roche Elecsys SARS-CoV-2 Ag).Results:The final population consisted in 93 subjects testing positive for SARS-CoV-2 RNA,34 with cycle threshold(Ct)values<29.5.The results of the two SARS-CoV-2 Ag immunoassays were significantly intercorrelated(r=0.77;P<0.001)in the entire cohort,though such correlation considerably improved in patients with high viral load(cycle threshold values<29.5:r=0.96;P<0.001).The accuracy for identifying samples with high viral load was excellent for both Lumipulse G SARS-CoV-2 Ag(AUC,0.99;P<0.001)and Elecsys SARS-CoV-2 Ag(AUC,0.99;P<0.001),with best cut-offs of 2.03 ng/mL for Lumipulse G SARS-CoV-2 Ag(1.00 sensitivity and 0.88 specificity)and 0.70 COI for Elecsys SARS-CoV-2 Ag(1.00 sensitivity and 0.80 specificity),respectively.Conclusion:The results of this study provide valuable support to usability of fully-automated,rapid,high throughput and accurate SARS-CoV-2 Ag immunoassays for complementing molecular assays. 展开更多
关键词 COVID-19 SARS-CoV-2 laboratory medicine diagnosis immunoassay
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3,6-Bis-β-Dicarbonylsubstituted Carbazoles Bearing N-Spacers and Their Eu(III) Complexes as Immunofluorescent Labelling Agents
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作者 Dmitry E. Pugachev Georgy V. Zatonsky +2 位作者 Tatyana S. Kostryukova Anna G. Shubina Nikolay V. Vasiliev 《International Journal of Organic Chemistry》 2024年第1期20-31,共12页
New reagents for immunofluorescence analysis of carbazole series containing fluorinated β-dicarbonyl fragments and carboxylic substituent groups separated by spacers of different lengths from the light-gathering carb... New reagents for immunofluorescence analysis of carbazole series containing fluorinated β-dicarbonyl fragments and carboxylic substituent groups separated by spacers of different lengths from the light-gathering carbazole scaffold have been developed. The markers in complex with Eu<sup>3+</sup> ions possess stability in the aqueous phase, intense and prolonged luminescence (τ 550 - 570 μs) with characteristic emission maxima in the region of 615 nm and excitation wavelengths in the region of 380 - 390 nm, which distinguishes them from most of the analogs used. In the study of marker conjugation with streptavidin, a reagent containing 4 - 5 europium labeling complexes based on spacer-containing carbazole tetraketone was obtained. The marker-doped silicate nanoparticles exhibit intense and long-lived luminescence in the characteristic region. 展开更多
关键词 Fluorescence immunoassay Fluorinated β-Diketones CARBAZOLE Europium Complexes STREPTAVIDIN Nanodispersions
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The Development of A Fluorescence Polarization Immunoassay for Aflatoxin Detection 被引量:11
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作者 SHENG Ya Jie EREMIN Sergei +3 位作者 MI Tie Jun ZHANG Su Xia SHEN Jian Zhong WANG Zhan Hui 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2014年第2期126-129,共4页
A fluorescence polarization immunoassay (FPIA) was developed for the analysis ofaflatoxins (AFs) using an anti-aflatoxin B1 (AFB1) monoclonal antibody and a novel fluorescein-labeled AFB1 tracer. The FPIA showed... A fluorescence polarization immunoassay (FPIA) was developed for the analysis ofaflatoxins (AFs) using an anti-aflatoxin B1 (AFB1) monoclonal antibody and a novel fluorescein-labeled AFB1 tracer. The FPIA showed an IC50 value of 23.33 ng/mL with a limit of detection of 13.12 ng/mL for AFB1. The cross-reactivities of AFB1, AFB2, AFG1, AFG2, AFM1, and AFM2 with the antibody were 100%, 65.7%, 143%, 23.5%, 111.4%, and 2%, respectively. The group-specificity of anti-AFB1mAb indicated that the FPIA could potentially be used in a screening method for the detection of total AFs, albeit not AFG2 and AFM2. The total time required for analyzing 96 samples in one microplate was less than 5 rain. This study demonstrates the potential usefulness of the FPIA as a rapid and simple technique for monitoring AFs. 展开更多
关键词 FPIA AFB The Development of A Fluorescence Polarization immunoassay for Aflatoxin Detection AFM EDF
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Detection of anti-preS1 antibodies for recovery of hepatitis B patients by immunoassay 被引量:15
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作者 Jun Wei Guang-Di Li Yuan Wang Zu-Chuan Zhang,Institute of Biochemsitry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China Yu-Qin Wang Zhi-Meng Lu,Department of Clinical virology,Rui-Jin Hospital,Shanghai Second Medical University 200025,Shanghai,China 《World Journal of Gastroenterology》 SCIE CAS CSCD 2002年第2期276-281,共6页
AIM: To establish a convenient immunoassay method based on recombinant antigen preS1(21-119aa) to detect anti-preS1 antibodies and evaluate the clinical significance of antibodies in hepatitis B. METHODS: The expressi... AIM: To establish a convenient immunoassay method based on recombinant antigen preS1(21-119aa) to detect anti-preS1 antibodies and evaluate the clinical significance of antibodies in hepatitis B. METHODS: The expression plasmid pET-28a-preS1 was constructed, and a large quantity of preS1(21-119aa) fragment of the large HBsAg protein was obtained. The preS1 fragment purified by Ni(2+)-IDA affinity chromatography was used as coated antigen to establish the indirect ELISA based on streptavidin-biotin system for detection of the anti-preS1 antibodies in sera from HBV-infected patients. For follow-up study, serial sera were collected during the clinical course of 21 HBV-infected patients and anti-preS1 antibodies, preS1 antigen, HBV-DNA and other serological HBV markers were analyzed. RESULTS: preS1(21-119aa) fragment was highly expressed from the plasmid pET-28a-preS1 in a soluble form in E.Coli (30mg.L(-1)), and easily purified to high purity over 90% by one step of Ni(2+)-IDA-sepharose 6B affinity chromatography. The purity and antigenicity of the purified preS1(21-119aa) protein was determined by 150g.L(-1) SDS-PAGE, Western blot and a direct ELISA. Recombinant preS1(21-119aa) protein was successfully applied in the immunoassay which could sensitively detect the anti-preS1 antibodies in serum specimens of acute or chronic hepatitis B patients. Results showed that more than half of 19 acute hepatitis B patients produced anti-preS1 antibodies during recovery of the disease, however, the response was only found in a few of chronic patients. In the clinical follow-up study of 11 patients with anti-preS1 positive serological profile, HBsAg and HBV-DNA clearance occurred in 6 of 10 acute hepatitis B patients in 5-6 months, and seroconversion of HBeAg and disappearance of HBV-DNA occurred in 1 chronic patients treated with lavumidine, a antiviral agent. CONCLUSION: The high-purity preS1(21-119aa) coated antigen was successfully prepared by gene expression and affinity chromatography. Using this antigen, a conveniently detective system of anti-preS1 antibodies in sera was established. Preliminarily clinical trial the occurrence of anti-preS1 antibodies in acute hepatitis B patients suggests the clearance of HBV from serum in a short-term time, and anti-preS1 positive in chronic patients means health improvement or recovery from the disease. 展开更多
关键词 Amino Acid Sequence ANTIBODIES Base Sequence Genetic Vectors Hepatitis B Hepatitis B Surface Antigens Humans immunoassay Molecular Sequence Data Peptide Fragments Protein Precursors Recombinant Fusion Proteins Research Support Non-U.S. Gov't Viral Envelope Proteins
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Detection of hepatitis B virus infection: A systematic review 被引量:9
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作者 Mallika Ghosh Srijita Nandi +1 位作者 Shrinwanti Dutta Malay Kumar Saha 《World Journal of Hepatology》 CAS 2015年第23期2482-2491,共10页
AIM: To review published methods for detection of hepatitis B virus(HBV) infection.METHODS: A thorough search on Medline database was conducted to find original articles describing different methods or techniques of d... AIM: To review published methods for detection of hepatitis B virus(HBV) infection.METHODS: A thorough search on Medline database was conducted to find original articles describing different methods or techniques of detection of HBV, which are published in English in last 10 years. Articles outlining methods of detection of mutants or drug resistance were excluded. Full texts and abstracts(if full text not available) were reviewed thoroughly. Manual search of references of retrieved articles were also done. We extracted data on different samples and techniques of detection of HBV, their sensitivity(Sn), specificity(Sp) and applicability.RESULTS: A total of 72 studies were reviewed. HBV was detected from dried blood/plasma spots, hepatocytes, ovarian tissue, cerumen, saliva, parotid tissue, renal tissue, oocytes and embryos, cholangiocarcinoma tissue, etc. Sensitivity of dried blood spot for detecting HBV was > 90% in all the studies. In case of seronegative patients, HBV DNA or serological markers have been detected from hepatocytes or renal tissue in many instances. Enzyme linked immunosorbent assay and Chemiluminescent immunoassay(CLIA) are most commonly used serological tests for detection. CLIA systems are also used for quantitation. Molecular techniques are used qualitatively as well as for quantitative detection. Among the molecular techniques version 2.0 of the Cobas Ampliprep/Cobas Taq Man assay and Abbott's real time polymerase chain reaction kit were found to be most sensitive with a lower detection limit of only 6.25 IU/m L and 1.48 IU/m L respectively. CONCLUSION: Serological and molecular assays are predominant and reliable methods for HBV detection. Automated systems are highly sensitive and quantify HBV DNA and serological markers for monitoring. 展开更多
关键词 Chemiluminescent immunoassay SEROLOGY AUTOMATED DE
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Chemiluminescence enzyme immunoassay based on magnetic nanoparticles for detection of hepatocellular carcinoma marker glypican-3 被引量:8
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作者 Qian-Yun Zhang Hui Chen +1 位作者 Zhen Lin Jin-Ming Lin 《Journal of Pharmaceutical Analysis》 SCIE CAS 2011年第3期166-174,共9页
Glypican-3 (GPC3) is reported as a great promising tumor marker for hepatocellular carcinoma (HCC) diagnosis. Highly sensitive and accurate analysis of serum GPC3 (sGPC3), in combination with or instead of tradi... Glypican-3 (GPC3) is reported as a great promising tumor marker for hepatocellular carcinoma (HCC) diagnosis. Highly sensitive and accurate analysis of serum GPC3 (sGPC3), in combination with or instead of traditional HCC marker alpha-fetoprotein (AFP), is essential for early diagnosis of I-ICC. Biomaterial-functionalized magnetic particles have been utilized as solid supports with good biological compatibility for sensitive immunoassay. Here, the magnetic nanoparticles (MnPs) and magnetic microparticles (MmPs) with carboxyl groups were further modified with streptavidin, and applied for the development of chemiluminescence enzyme immunoassay (CLEIA). After comparing between MnPs- and MmPs-based CLEIA, MnPs-based CLEIA was proved to be a better method with less assay time, greater sensitivity, better linearity and longer chemiluminescence platform. MnPs-based CLEIA was applied for detection of sGPC3 in normal liver, hepatocirrhosis, secondary liver cancer and HCC serum samples. The results indicated that sGPC3 was effective in diagnosis of HCC with high performance. 展开更多
关键词 Magnetic nanoparticle Magnetic microparticleChemiluminescenceenzyme immunoassay GLYPICAN-3 Hepatocellularcarcinoma
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Hierarchical Nanogold Labels to Improve the Sensitivity of Lateral Flow Immunoassay 被引量:6
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作者 Kseniya Serebrennikova Jeanne Samsonova Alexander Osipov 《Nano-Micro Letters》 SCIE EI CAS 2018年第2期67-74,共8页
Lateral flow immunoassay(LFIA) is a widely used express method and offers advantages such as a short analysis time, simplicity of testing and result evaluation.However, an LFIA based on gold nanospheres lacks the desi... Lateral flow immunoassay(LFIA) is a widely used express method and offers advantages such as a short analysis time, simplicity of testing and result evaluation.However, an LFIA based on gold nanospheres lacks the desired sensitivity, thereby limiting its wide applications.In this study, spherical nanogold labels along with new types of nanogold labels such as gold nanopopcorns and nanostars were prepared, characterized, and applied for LFIA of model protein antigen procalcitonin. It was found that the label with a structure close to spherical provided more uniform distribution of specific antibodies on its surface, indicative of its suitability for this type of analysis.LFIA using gold nanopopcorns as a label allowed procalcitonin detection over a linear range of 0.5–10 ng mL^(-1) with the limit of detection of 0.1 ng mL^(-1), which was fivefold higher than the sensitivity of the assay with gold nanospheres. Another approach to improve the sensitivity of the assay included the silver enhancement method,which was used to compare the amplification of LFIA for procalcitonin detection. The sensitivity of procalcitonin determination by this method was 10 times better the sensitivity of the conventional LFIA with gold nanosphere as a label. The proposed approach of LFIA based on gold nanopopcorns improved the detection sensitivity without additional steps and prevented the increased consumption of specific reagents(antibodies). 展开更多
关键词 Lateral flow immunoassay Gold nanosphere Gold nanopopcorn Gold nanostar Silver enhancement PROCALCITONIN
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Comparison of chemiluminescence enzyme immunoassay based on magnetic microparticles with traditional colorimetric ELISA for the detection of serum α-fetoprotein 被引量:5
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作者 Qian-Yun Zhang a,b,Hui Chen a,Zhen Lin a,Jin-Ming Lin a a Beijing Key Laboratory of Microanalytical Methods and Instrumentation,Department of Chemistry,Tsinghua University,Beijing 100029,China b Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China 《Journal of Pharmaceutical Analysis》 SCIE CAS 2012年第2期130-135,共6页
A chemiluminescence enzyme immunoassay based on magnetic microparticles (MmPs-CLEIA) was developed to evaluate serum a-fetoprotein (AFP) in parallel with traditional colorimetric enzyme-linked immunosorbent assay (ELI... A chemiluminescence enzyme immunoassay based on magnetic microparticles (MmPs-CLEIA) was developed to evaluate serum a-fetoprotein (AFP) in parallel with traditional colorimetric enzyme-linked immunosorbent assay (ELISA).A systematic comparison between the MmPs-CLEIA and colorimetric ELISA concluded that the MPs-CLEIA exhibited fewer dosages of immunoreagents,less total assay time,and better linearity,recovery,precision,sensitivity and validity.AFP was detected in forty human serum samples by the proposed MPs-CLEIA and ELISA,and the results were compared with commercial electrochemiluminescence immunoassay (ECLIA) kit.The correlation coefficient between MPs-CLEIA and ELISA was obtained with R 2 0.6703;however,the correlation between MPs-CLEIA and ECLIA (R 2 0.9582) was obviously better than that between colorimetric ELISA and ECLIA (R 2 0.6866). 展开更多
关键词 a-Fetoprotein Hepatocellular carcinoma Chemiluminescence enzyme immunoassay Magnetic microparticles Colorimetric enzyme-linked immunosorbent assay
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Immunoassay of chemical contaminants in milk:A review 被引量:4
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作者 XU Fei REN Kang +5 位作者 YANG Yu-ze GUO Jiang-peng MA Guang-peng LIU Yi-ming LU Yong-qiang LI Xiu-bo 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2015年第11期2282-2295,共14页
The detection of chemical contaminants is critical to ensure dairy safety. These contaminants include veterinary medicines, antibiotics, pesticides, heavy metals, mycotoxins, and persistent organic pollutants (POPs)... The detection of chemical contaminants is critical to ensure dairy safety. These contaminants include veterinary medicines, antibiotics, pesticides, heavy metals, mycotoxins, and persistent organic pollutants (POPs). Immunoassays have recently been used to detect contaminants in milk because of their simple operation, high speed, and low cost. This article describes the latest developments in the most important component of immunoassays--antibodies, and then reviews the four major substrates used for immunoassays (i.e., microplates, membranes, gels, and chips) as well as their use in the detection of milk contaminants. The paper concludes with prospects for further aDDlications of these immunoassavs. 展开更多
关键词 MILK chemical contaminants immunoassay ANTIBODIES
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A New Hapten for Immunoassay of Aldicarb 被引量:3
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作者 Yan Feng ZHANG Zhi Xian GAO +1 位作者 Qing Min ZHANG Shu Gui DAI 《Chinese Chemical Letters》 SCIE CAS CSCD 2006年第8期1021-1024,共4页
A new hapten, aldicarb oxime succinic ester (AOSE), was synthesized for immunoassay of aldicarb. It was conjugated to proteins by active ester method. Polyclonal antibody was raised against AOSE-BSA (bovine serum a... A new hapten, aldicarb oxime succinic ester (AOSE), was synthesized for immunoassay of aldicarb. It was conjugated to proteins by active ester method. Polyclonal antibody was raised against AOSE-BSA (bovine serum albumin) conjugate. Enzyme-linked immunosorbent assays (ELISAs) showed that this antiserum had high affinity to aldicarb and can be used for sensitive and selective immunoassay of aldicarb. 展开更多
关键词 ALDICARB immunoassay HAPTEN analysis.
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Characterization of Hapten-Protein Conjugates for Immunoassay of Polycyclic Aromatic Hydrocarbons(PAHs) 被引量:3
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作者 ZHANG Yan-feng GAO Zhi-xian +1 位作者 SUN Hong-wen DAI Shu-gui 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2008年第6期697-700,共4页
Preparation and characterization of the hapten-protein conjugates are fundamental to developing environmental immunoassays. As a hapten, 1-pyrenebutyric acid(PBA) was conjugated to the carrier protein of bovine seru... Preparation and characterization of the hapten-protein conjugates are fundamental to developing environmental immunoassays. As a hapten, 1-pyrenebutyric acid(PBA) was conjugated to the carrier protein of bovine serum albumin(BSA) or ovalbumin(OVA) by active ester method. Infrared spectra(IR) showed that PBA-BSA and PBA-OVA conjugates were successfully prepared. The number of the haptens conjugated to the carrier protein was determined by ultraviolet spectra(UV) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF-MS). The calculated average binding ratios of PBA/BSA and PBA/OVA were 18:1 and 10:1 by UV, and 31:1 and 22:1 by MALDI-TOF-MS, respectively. Although there was a discrepancy between the results determined by the two methods, both of them were useful for the characterization of the hapten-protein conjugates. The antibody was produced against the antigen of PBA-BSA, and the affinity was tested by the double agar diffusion method The conjugates and the antibody could be used for developing a sensitive and selective immunoassay of polycyclic aromatic hydrocarbons(PAHs). 展开更多
关键词 Polycyclic aromatic hydrocarbons HAPTEN Conjugate immunoassay CHARACTERIZATION
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Lateral Flow Immunoassay for Quantitative Detection of Ractopamine in Swine Urine 被引量:3
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作者 REN Mei Ling CHEN Xue Lan +4 位作者 LI Chao Hui XU Bo LIU Wen Juan XU Heng Yi XIONG Yong Hua 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2014年第2期134-137,共4页
A strip reader based lateral flow immunoassay (LFIA) was established for the rapid and quantitative detection of ractopamine (RAC) in swine urine. The ratio of the optical densities (ODs) of the test line (AT)... A strip reader based lateral flow immunoassay (LFIA) was established for the rapid and quantitative detection of ractopamine (RAC) in swine urine. The ratio of the optical densities (ODs) of the test line (AT) to that of the control line (Ac) was used to effectively minimize interference among strips and sample variations. The linear range for the quantitative detection of RAC was 0.2 ng/mL to 3.5 ng/mL with a median inhibitory concentration (IC50) of 0.59+0.06 ng/mL. The limit of detection (LOD) of the LFIA was 0.13 ng/mL. The intra-assay recovery rates were 92.97%, 97.25%, and 107.41%, whereas the inter-assay rates were 80.07%, 108.17%, and 93.7%, respectively. 展开更多
关键词 RAC Lateral Flow immunoassay for Quantitative Detection of Ractopamine in Swine Urine FIGURE AT
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Rapid and Sensitive Chemiluminescent Enzyme Immunoassay for the Determination of Neomycin Residues in Milk 被引量:5
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作者 LUO Peng Jie ZHANG Jian Bo +8 位作者 WANG Hua Li CHEN Xia WU Nan ZHAO Yun Feng WANG Xiao Mei ZHANG Hong ZHANG Ji Yue ZHU Lei JIANG Wen Xiao 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2016年第5期374-378,共5页
Immunoassays greatly contribute to veterinary drug residue analysis. However, there are few reports on detecting neomycin residues by immunoassay. Here, a rapid and sensitive chemiluminescent enzyme immunoassay (CLIE... Immunoassays greatly contribute to veterinary drug residue analysis. However, there are few reports on detecting neomycin residues by immunoassay. Here, a rapid and sensitive chemiluminescent enzyme immunoassay (CLIEA) was successfully developed for neomycin residue analysis. CLIEA demonstrated good cross-reactivity for neomycin, and the IC50 value was 2.4 ng/mL in buffer. 展开更多
关键词 CLEIA Rapid and Sensitive Chemiluminescent Enzyme immunoassay for the Determination of Neomycin Residues in Milk
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Determination of theophylline concentration in serum by chemiluminescent immunoassay 被引量:2
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作者 周美霞 管茶英 +2 位作者 陈光 谢鑫友 吴盛海 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2005年第12期1148-1152,共5页
Objective: This study aimed to establish chemiluminescent immunoassay (CLIA) for quantitative determination of theophylline levels in human serum. Methods: To measure the concentration of theophylline (n=122) and eval... Objective: This study aimed to establish chemiluminescent immunoassay (CLIA) for quantitative determination of theophylline levels in human serum. Methods: To measure the concentration of theophylline (n=122) and evaluate the assay.Results: The linear range of the CLIA method was 0.51~40 mg/L (Y=1.02X+0.44, r=0.995). The intra and inter CV (coefficient variance) of CLIA were 3.20% and 3.57%, respectively. The average recovery rate was 102.3%. This method was free from interference by brilirubin (<200 μmol/L), hemoglobin (<10 g/L), and triglycerides (<15 mmol/L). Conclusion: This method is simple, convenient and precise for clinical pharmacokinetics study oftheophylline. 展开更多
关键词 THEOPHYLLINE Chemiluminescent immunoassay (CLIA) Fluorescence polarization immunoassay (FPIA)
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Development of Lateral-flow Immunoassay for WSSV with Polyclonal Antibodies Raised against Recombinant VP (19+28) Fusion Protein 被引量:7
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作者 Qing-yu CHENG Xiao-lin MENG Jin-ping XU Wei LU Jian WANG 《中国病毒学》 CSCD 2007年第1期61-67,共7页
我们为 WSSV 开发了敏感、快速的侧面流动的免疫分析(LFIA ) ,用象指示物的胶体的黄金。熔化蛋白质, VP (19+28 ) ,在 E 被表示。coli,净化并且过去常准备 polyclonal 抗体。净化的 anti-VP (19+28 ) IgG 与胶体的黄金被结合。Uncon... 我们为 WSSV 开发了敏感、快速的侧面流动的免疫分析(LFIA ) ,用象指示物的胶体的黄金。熔化蛋白质, VP (19+28 ) ,在 E 被表示。coli,净化并且过去常准备 polyclonal 抗体。净化的 anti-VP (19+28 ) IgG 与胶体的黄金被结合。Unconjugated anti-VP (19+28 ) IgG 和山羊反兔子 IgG 在硝化纤维素膜上被使不能调动。在汇编以后,虾样品的三个组(在每个组的 5 个单个动物) 被测试它包括了健康、垂死、死了的虾。为每个组,三不同纸巾(身体蜜汁,鳃和 hepatopancreas ) 同时被测试。在平行,所有样品也为比较用 PCR 被分析。从测试的 45 件样品,当 15 作为否定被分类时, 30 作为积极被检测。LFIA 的结果相关,那些由 PCR 分析获得了,显示这二个察觉方法有在在这初步的研究测试的样品的有限数字的一样的功效。关键词侧面流动的免疫分析 - 白点症候群病毒(WSSV )- VP19 - VP28 CLC 数字 S945. 展开更多
关键词 Lateral-flow immunoassay White spot syndrome virus (WSSV) VP19 VP28
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Current molecular methods for the detection of hepatitis C virus in high risk group population:A systematic review 被引量:4
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作者 Rushna Firdaus Kallol Saha +1 位作者 Aritra Biswas Provash Chandra Sadhukhan 《World Journal of Virology》 2015年第1期25-32,共8页
Hepatitis C virus(HCV) is an emerging infection worldwide and the numbers of persons infected are increasing every year. Poor blood transfusion methods along with unsafe injection practices are potential sources for t... Hepatitis C virus(HCV) is an emerging infection worldwide and the numbers of persons infected are increasing every year. Poor blood transfusion methods along with unsafe injection practices are potential sources for the rapid spread of infection. Early detection of HCV is the need of the hour especially in high riskgroup population as these individuals are severely immunocompromised. Enzyme Immunoassays are the most common detection techniques but they provide no evidence of active viremia or identification of infected individuals in the antibody-negative phase and their efficacy is limited in individuals within high risk group population. Molecular virological techniques have an important role in detecting active infection with utmost specificity and sensitivity. Technologies for assessment of HCV antibody and RNA levels have improved remarkably, as well as our understanding of how to best use these tests in patient management. This review aims to give an overview of the different serological and molecular methods employed in detecting HCV infection used nowadays. Additionally, the review gives an insight in the new molecular techniques that are being developed to improve the detection techniques particularly in High Risk Group population who are severely immunocompromised. 展开更多
关键词 Molecular DETECTION Enzyme immunoassay High risk group population Nucleic acid amplification assays POLYMERASE chain reaction
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