Castration of male animals is a common practice in the meat industry aimed at reducing aggressive behavior, preventing unpleasant flavor, and controlling undesirable breeding. For many years, mechanical castration and...Castration of male animals is a common practice in the meat industry aimed at reducing aggressive behavior, preventing unpleasant flavor, and controlling undesirable breeding. For many years, mechanical castration and surgical castration have been practiced to sterilize the animals. However, these castration methods are not humane because of the associated risk of death, pain, and stress. Recently, immunocastration targeting the hypothalamic-pituitary-gonadal axis(HPG) axis has been reported as an animal-friendly approach that circumvents many of the concerns with conventional castration, and suggested by researches as an alternative to surgical castration. However, there is no compilation of updated information on the use of immunocastration in sheep and goats. Therefore, this review aims to summarize the developmental process from traditional surgical castration to immunocastration and to screen the process of immune targets. It also compares the respective advantages and disadvantages of traditional castration technologies and immunocastration, in particular including analyses in the characteristics, features application and welfare benefits of immunocastration in sheep and goats were also analyzed.展开更多
The objective of the present study was to evaluate the effect of an immunogenic maltose-binding protein-gonadotropin releasing hormone （GnRH-6-MBP） using genetic engineering. The synthetic mammalian tandem repeated ...The objective of the present study was to evaluate the effect of an immunogenic maltose-binding protein-gonadotropin releasing hormone （GnRH-6-MBP） using genetic engineering. The synthetic mammalian tandem repeated GnRH hexamer gene was inserted into the expression plasmid pMAL-c2x. Recombinant GnRH-6-MBP protein was over- expressed in E.coli strain BL21. Amylose resin with affinity chromatograph was used to purify target protein. The reactiongenicity of fusion protein was identified by indirect enzyme linked immunosorbent assay （ELISA）, and the antigenicity and biological effects of GnRH-6-MBP were tested in mice. In the experiment, 20 male Kunming white mice of 20 d old were randomly divided into treatment and control group. Ten mice were immunized with 100 μg GnRH-6-MBP administered subcutaneously （s.c.） thrice at 2-week intervals with GnRH-6-MBP. Mice were sacrificed after 3 weeks following the booster injection, the testis was removed, weighed and measured, and the histological structure was observed. The reactiongenicity of fusion protein to GnRH antibody was much higher than the control. Active immunization against GnRH-6-MBP reduced remarkably （P 〈 0.01） the length and weight of the testis, and shortened the girth and width of the testis （P 〈 0.05）, and suppressed testicular spermatogenesis compared to the control mice. These results indicate that the recombinant GnRH-6-MBP acted as a strong immunogen and caused atrophy of the testis.展开更多
基金funded by the National Natural Science Foundation of China(31972997 and 31772598)the China Agriculture Research System of MOF and MARA(CARS-38)。
文摘Castration of male animals is a common practice in the meat industry aimed at reducing aggressive behavior, preventing unpleasant flavor, and controlling undesirable breeding. For many years, mechanical castration and surgical castration have been practiced to sterilize the animals. However, these castration methods are not humane because of the associated risk of death, pain, and stress. Recently, immunocastration targeting the hypothalamic-pituitary-gonadal axis(HPG) axis has been reported as an animal-friendly approach that circumvents many of the concerns with conventional castration, and suggested by researches as an alternative to surgical castration. However, there is no compilation of updated information on the use of immunocastration in sheep and goats. Therefore, this review aims to summarize the developmental process from traditional surgical castration to immunocastration and to screen the process of immune targets. It also compares the respective advantages and disadvantages of traditional castration technologies and immunocastration, in particular including analyses in the characteristics, features application and welfare benefits of immunocastration in sheep and goats were also analyzed.
基金the support from the Natural Science Foundation of Anhui Provincial Education Department, China (KJ2007B175)
文摘The objective of the present study was to evaluate the effect of an immunogenic maltose-binding protein-gonadotropin releasing hormone （GnRH-6-MBP） using genetic engineering. The synthetic mammalian tandem repeated GnRH hexamer gene was inserted into the expression plasmid pMAL-c2x. Recombinant GnRH-6-MBP protein was over- expressed in E.coli strain BL21. Amylose resin with affinity chromatograph was used to purify target protein. The reactiongenicity of fusion protein was identified by indirect enzyme linked immunosorbent assay （ELISA）, and the antigenicity and biological effects of GnRH-6-MBP were tested in mice. In the experiment, 20 male Kunming white mice of 20 d old were randomly divided into treatment and control group. Ten mice were immunized with 100 μg GnRH-6-MBP administered subcutaneously （s.c.） thrice at 2-week intervals with GnRH-6-MBP. Mice were sacrificed after 3 weeks following the booster injection, the testis was removed, weighed and measured, and the histological structure was observed. The reactiongenicity of fusion protein to GnRH antibody was much higher than the control. Active immunization against GnRH-6-MBP reduced remarkably （P 〈 0.01） the length and weight of the testis, and shortened the girth and width of the testis （P 〈 0.05）, and suppressed testicular spermatogenesis compared to the control mice. These results indicate that the recombinant GnRH-6-MBP acted as a strong immunogen and caused atrophy of the testis.
