BACKGROUND Gastric glomus tumor(GGT)is rare submucosal mesenchymal tumor that lacks specific clinical manifestations and is usually treated mainly by traditional surgical resection.This paper presents a case of a GGT,...BACKGROUND Gastric glomus tumor(GGT)is rare submucosal mesenchymal tumor that lacks specific clinical manifestations and is usually treated mainly by traditional surgical resection.This paper presents a case of a GGT,exhibited both intraluminally and extraluminally growth that was removed by laparoscopy-gastroscopy cooperative surgery.CASE SUMMARY A 52-year-old male presented with epigastric discomfort accompanied by a sense of fullness for 3 mo.Upper gastrointestinal endoscopy identified a submucosal lump located in the gastric antrum.Endoscopic ultrasonography identified a 2.4 cm×1.8 cm lump located in the gastric antrum.It originated from the muscularis propria and exhibited both intraluminally and extraluminally growth,with hypoechoicity on the periphery,hyperechoicity in the middle,and unclear boundaries.Computed tomography showed nodular thickening of 3.0 cm×2.2 cm in the gastric wall of the gastric antrum,and after enhancement,the lesion exhibited obvious enhancement We suspected that it was a gastrointestinal stromal tumor(glomus tumor and schwannoma were not excluded)and planned to perform laparoscopy-gastroscopy cooperative surgery.Immunohistochemical staining after the operation revealed that spinal muscular atrophy(+),hcaldesmon(+),cluster of differentiation 34(CD34)(+),2%Ki-67-positive rate,CD56,melanoma antigen,CD117,discovered on GIST-1,leukocyte common antigen,caudal type homeobox 2,cytokeratin,and S-100 were all negative.The tumor was finally diagnosed as a GGT.CONCLUSION GGTs are rare submucosal tumors of the stomach and should be considered in the differential diagnosis of gastric submucosal tumors.Laparoscopy-gastroscopy cooperative surgery is less invasive and more precise and could be an effective method for the treatment of GGTs.展开更多
BACKGROUND Schwannomas,also known as neurinomas,are benign tumors derived from Schwann cells.Gastrointestinal schwannomas are rare and are most frequently reported in the stomach.They are usually asymptomatic and are ...BACKGROUND Schwannomas,also known as neurinomas,are benign tumors derived from Schwann cells.Gastrointestinal schwannomas are rare and are most frequently reported in the stomach.They are usually asymptomatic and are difficult to diagnose preoperatively;however,endoscopy and imaging modalities can provide beneficial preliminary diagnostic data.There are various surgical options for management.Here,we present a case of a large gastric schwannoma(GS)managed by combined laparoscopic and endoscopic surgery.CASE SUMMARY A 28-year-old woman presented with a 2-mo history of epigastric discomfort and a feeling of abdominal fullness.On upper gastrointestinal endoscopy and endoscopic ultrasonography,a hypoechogenic submucosal mass was detected in the gastric antrum:It emerged from the muscularis propria and projected intraluminally.Computed tomography showed a nodular lesion(4 cm×3.5 cm),which exhibited uniform enhancement,on the gastric antrum wall.Based on these findings,a preliminary diagnosis of gastrointestinal stromal tumor was established,with schwannoma as a differential.Considering the large tumor size,we planned to perform endoscopic resection and to convert to laparoscopic treatment,if necessary.Eventually,the patient underwent combined laparoscopic and gastroscopic surgery.Immunohistochemically,the resected specimen showed positivity for S-100 and negativity for desmin,DOG-1,α-smooth muscle actin,CD34,CD117,and p53.The Ki-67 index was 3%,and a final diagnosis of GS was established.CONCLUSION Combined laparoscopic and endoscopic surgery is a minimally invasive and effective treatment option for large GSs.展开更多
Objective:To evaluate the expression and correlation of hypoxia inducible factor 1α(HIF-1α)and vascular endothelial growth factor(VEGF)and Survivin proteins in biopsy specimens of esophageal squamous cell carcinoma(...Objective:To evaluate the expression and correlation of hypoxia inducible factor 1α(HIF-1α)and vascular endothelial growth factor(VEGF)and Survivin proteins in biopsy specimens of esophageal squamous cell carcinoma(ESCC), and then determine whether the levels of expression of these proteins could predict the clinical effectiveness of radiotherapy in individual cancers.Methods:The expressions of HIF-1α,VEGF and Survivin were shown by S-P immunohistochemical stainingmethodinbiopsyspecimensofESCC,whichwereobtainedendoscopicallyfrom50patientsbeforeradiotherapy,and 10 cases of normal esophageal tissue.Results:The positive expression rates of HIF-1α,VEGF and Survivin were 68%,74% and72%inESCCrespectively.However,thethreetumormarkershadnegativeexpressionsinnormalesophagealtissue.The positive rate of HIF-1αwas positively correlated with VEGF and Survivin proteins.The positive rates of HIF-1αand Survivin were closely related to the clinical stage,radiotherapy effectiveness and survival,otherwise,the expression of HIF-1αwas closely related to distant metastasis;both of them were no correlation with the differentiation degree of tumor.The effective rates of radiotherapy and mean survival periods of those cases with positive and negative expressions of HIF-1αwere 8.8%, 10 months and 81.25%,25 months,respectively.The one,two,and three years survival rates of patients with positive and negative expressions of HIF-1αwere 38.2%,5.9%,2.9%,and 81.3%,54.2%,15.8%,respectively(P=0.001).Patients with HIF-1αpositive expression obviously survived less than those with negative expression and the difference was significant. The expression of VEGF was only related to the distant metastasis.Conclusion:Over expressions of HIF-1α,VEGF and Survivin were found in ESCC.The positive rate of HIF-1αwas positively correlated with VEGF and Survivin proteins.The expression of HIF-1αmay serve as an important parameter in evaluating response for radiotherapy and prognosis of ESCC. It may play an important role by up-regulating the transcription of VEGF and Survivin genes.展开更多
[Objectives]To explore the expression of interleukin 34(IL-34)in gastric adenocarcinoma tissues and its relationship with apoptosis of gastric adenocarcinoma cells.[Methods]60 cases of surgically resected human gastri...[Objectives]To explore the expression of interleukin 34(IL-34)in gastric adenocarcinoma tissues and its relationship with apoptosis of gastric adenocarcinoma cells.[Methods]60 cases of surgically resected human gastric adenocarcinoma tissue specimen and 50 cases of adjacent normal gastric mucosa tissue specimen were collected,and the expression of IL-34 protein was determined by immunohistochemical streptavidin-perosidase(SP)method.Cell apoptosis in tissue specimen was detected by TUNEL staining method,and the relationship between IL-34 protein expression and apoptosis of gastric adenocarcinoma cells was analyzed.[Results]Immunohistochemical SP experiment indicated that the expression of IL-34 protein in gastric adenocarcinoma was higher than that in adjacent normal gastric mucosa(P<0.05);its positive expression was related to histological differentiation,TNM stage,invasion depth,and lymph node metastasis of gastric adenocarcinoma(P<0.05),but not to gender and age(P>0.05).TUNEL experiment showed that compared with the adjacent normal gastric mucosa group,the apoptosis index(AI)of gastric adenocarcinoma group was significantly lower(P<0.05);the AI was related to histological differentiation,TNM stage,tumor invasion depth and lymph node metastasis of gastric adenocarcinoma(P<0.05),but not to gender and age(P>0.05).In gastric adenocarcinoma,the AI of IL-34 positive expression group was lower than that of IL-34 negative expression group,and the results were statistically significant(P<0.05).[Conclusions]IL-34 has high expression in gastric adenocarcinoma tissues and is negatively correlated with cancer cell apoptosis.Abnormal expression of IL-34 is involved in the occurrence and development of gastric adenocarcinoma.This provides a new idea for the pathogenesis research and clinical treatment of gastric adenocarcinoma.展开更多
基金Key Program of Shandong Provincial Natural Science Foundation,No.ZR2020KE018The Project of Shandong Province Higher Educational Science and Technology Program,No.J17KA253+1 种基金The Medical Science and Technology Development Project of Shandong,No.2018WS062Weifang Science and Technology Development Plan,No.2020YX040.
文摘BACKGROUND Gastric glomus tumor(GGT)is rare submucosal mesenchymal tumor that lacks specific clinical manifestations and is usually treated mainly by traditional surgical resection.This paper presents a case of a GGT,exhibited both intraluminally and extraluminally growth that was removed by laparoscopy-gastroscopy cooperative surgery.CASE SUMMARY A 52-year-old male presented with epigastric discomfort accompanied by a sense of fullness for 3 mo.Upper gastrointestinal endoscopy identified a submucosal lump located in the gastric antrum.Endoscopic ultrasonography identified a 2.4 cm×1.8 cm lump located in the gastric antrum.It originated from the muscularis propria and exhibited both intraluminally and extraluminally growth,with hypoechoicity on the periphery,hyperechoicity in the middle,and unclear boundaries.Computed tomography showed nodular thickening of 3.0 cm×2.2 cm in the gastric wall of the gastric antrum,and after enhancement,the lesion exhibited obvious enhancement We suspected that it was a gastrointestinal stromal tumor(glomus tumor and schwannoma were not excluded)and planned to perform laparoscopy-gastroscopy cooperative surgery.Immunohistochemical staining after the operation revealed that spinal muscular atrophy(+),hcaldesmon(+),cluster of differentiation 34(CD34)(+),2%Ki-67-positive rate,CD56,melanoma antigen,CD117,discovered on GIST-1,leukocyte common antigen,caudal type homeobox 2,cytokeratin,and S-100 were all negative.The tumor was finally diagnosed as a GGT.CONCLUSION GGTs are rare submucosal tumors of the stomach and should be considered in the differential diagnosis of gastric submucosal tumors.Laparoscopy-gastroscopy cooperative surgery is less invasive and more precise and could be an effective method for the treatment of GGTs.
基金Supported by Zhejiang Provincial Natural Science Foundation of China,No. LGF18H160036
文摘BACKGROUND Schwannomas,also known as neurinomas,are benign tumors derived from Schwann cells.Gastrointestinal schwannomas are rare and are most frequently reported in the stomach.They are usually asymptomatic and are difficult to diagnose preoperatively;however,endoscopy and imaging modalities can provide beneficial preliminary diagnostic data.There are various surgical options for management.Here,we present a case of a large gastric schwannoma(GS)managed by combined laparoscopic and endoscopic surgery.CASE SUMMARY A 28-year-old woman presented with a 2-mo history of epigastric discomfort and a feeling of abdominal fullness.On upper gastrointestinal endoscopy and endoscopic ultrasonography,a hypoechogenic submucosal mass was detected in the gastric antrum:It emerged from the muscularis propria and projected intraluminally.Computed tomography showed a nodular lesion(4 cm×3.5 cm),which exhibited uniform enhancement,on the gastric antrum wall.Based on these findings,a preliminary diagnosis of gastrointestinal stromal tumor was established,with schwannoma as a differential.Considering the large tumor size,we planned to perform endoscopic resection and to convert to laparoscopic treatment,if necessary.Eventually,the patient underwent combined laparoscopic and gastroscopic surgery.Immunohistochemically,the resected specimen showed positivity for S-100 and negativity for desmin,DOG-1,α-smooth muscle actin,CD34,CD117,and p53.The Ki-67 index was 3%,and a final diagnosis of GS was established.CONCLUSION Combined laparoscopic and endoscopic surgery is a minimally invasive and effective treatment option for large GSs.
基金a grant from the tackle key problems in science andtechnology of Hebei Province(No.052761764).
文摘Objective:To evaluate the expression and correlation of hypoxia inducible factor 1α(HIF-1α)and vascular endothelial growth factor(VEGF)and Survivin proteins in biopsy specimens of esophageal squamous cell carcinoma(ESCC), and then determine whether the levels of expression of these proteins could predict the clinical effectiveness of radiotherapy in individual cancers.Methods:The expressions of HIF-1α,VEGF and Survivin were shown by S-P immunohistochemical stainingmethodinbiopsyspecimensofESCC,whichwereobtainedendoscopicallyfrom50patientsbeforeradiotherapy,and 10 cases of normal esophageal tissue.Results:The positive expression rates of HIF-1α,VEGF and Survivin were 68%,74% and72%inESCCrespectively.However,thethreetumormarkershadnegativeexpressionsinnormalesophagealtissue.The positive rate of HIF-1αwas positively correlated with VEGF and Survivin proteins.The positive rates of HIF-1αand Survivin were closely related to the clinical stage,radiotherapy effectiveness and survival,otherwise,the expression of HIF-1αwas closely related to distant metastasis;both of them were no correlation with the differentiation degree of tumor.The effective rates of radiotherapy and mean survival periods of those cases with positive and negative expressions of HIF-1αwere 8.8%, 10 months and 81.25%,25 months,respectively.The one,two,and three years survival rates of patients with positive and negative expressions of HIF-1αwere 38.2%,5.9%,2.9%,and 81.3%,54.2%,15.8%,respectively(P=0.001).Patients with HIF-1αpositive expression obviously survived less than those with negative expression and the difference was significant. The expression of VEGF was only related to the distant metastasis.Conclusion:Over expressions of HIF-1α,VEGF and Survivin were found in ESCC.The positive rate of HIF-1αwas positively correlated with VEGF and Survivin proteins.The expression of HIF-1αmay serve as an important parameter in evaluating response for radiotherapy and prognosis of ESCC. It may play an important role by up-regulating the transcription of VEGF and Survivin genes.
基金Supported by Cultivation Fund of National Natural Science Foundation of China Project(202114)Major Program at School Level(201711).
文摘[Objectives]To explore the expression of interleukin 34(IL-34)in gastric adenocarcinoma tissues and its relationship with apoptosis of gastric adenocarcinoma cells.[Methods]60 cases of surgically resected human gastric adenocarcinoma tissue specimen and 50 cases of adjacent normal gastric mucosa tissue specimen were collected,and the expression of IL-34 protein was determined by immunohistochemical streptavidin-perosidase(SP)method.Cell apoptosis in tissue specimen was detected by TUNEL staining method,and the relationship between IL-34 protein expression and apoptosis of gastric adenocarcinoma cells was analyzed.[Results]Immunohistochemical SP experiment indicated that the expression of IL-34 protein in gastric adenocarcinoma was higher than that in adjacent normal gastric mucosa(P<0.05);its positive expression was related to histological differentiation,TNM stage,invasion depth,and lymph node metastasis of gastric adenocarcinoma(P<0.05),but not to gender and age(P>0.05).TUNEL experiment showed that compared with the adjacent normal gastric mucosa group,the apoptosis index(AI)of gastric adenocarcinoma group was significantly lower(P<0.05);the AI was related to histological differentiation,TNM stage,tumor invasion depth and lymph node metastasis of gastric adenocarcinoma(P<0.05),but not to gender and age(P>0.05).In gastric adenocarcinoma,the AI of IL-34 positive expression group was lower than that of IL-34 negative expression group,and the results were statistically significant(P<0.05).[Conclusions]IL-34 has high expression in gastric adenocarcinoma tissues and is negatively correlated with cancer cell apoptosis.Abnormal expression of IL-34 is involved in the occurrence and development of gastric adenocarcinoma.This provides a new idea for the pathogenesis research and clinical treatment of gastric adenocarcinoma.
