Objective: To investigate the expression of Smad4 and p21WAF1 in endometrial carcinoma and its clinical significance. Methods: Immunohistochemical method wasused to detect Smad4 and p21WAF1 expression in 56 cases of ...Objective: To investigate the expression of Smad4 and p21WAF1 in endometrial carcinoma and its clinical significance. Methods: Immunohistochemical method wasused to detect Smad4 and p21WAF1 expression in 56 cases of endometrial carcinoma. Results: The positive rate ofSmad4 was 80.36% in endometrial carcinoma. The Samd4 expression was significantly correlated with histologicalgrade (P<0.01) and clinical stage (P<0.05). The positive rate of p21WAF1 was 64.28% in endometrial carcinoma. Theexpression of p21WAF1 was correlated with depth ofmyometrial invasion (P<0.01). There was no correlation between Smad4 and p21WAF1 expression (P>0.05). Conclusion: Smad4 may play an important role in the tumorigenesis, differentiation and progression ofendometrial carcinoma. The expression of p21WAF1 wasassociated with the tumorigenesis of endometrial carcinoma, but the association between p21WAF1 and differentiationand progression of endometrial carcinomas needs to befurther investigated.展开更多
Objective To study the role of p16 and cyclin D 1 in the genesis and development of endometrial carcinoma.Methods 12 cases of normal endometrium,22 cases of proliferative endometrium and 41 cases of endometrial carcin...Objective To study the role of p16 and cyclin D 1 in the genesis and development of endometrial carcinoma.Methods 12 cases of normal endometrium,22 cases of proliferative endometrium and 41 cases of endometrial carcinoma were detected for the expression of p16 and cyclin D 1 by means of immunohistochemical S P. Results In normal endometrium p16 was expressed while cyclin D 1 was almost negative in the proliferative phase,but both of them were negative in the secretory phase.Among the groups of the simple and compound hyperplasia, the atypical hyperplasia and the endometrial carcinoma,the expression of p16 showed a descending tendency, while the expression of cyclin D 1 showed an ascending tendency.In endometrial carcinomas the expression of p16 was significantly lower than that of normal endometrium and proliferative endometrium( P <0.01, P <0.05).However, the expression of cyclin D 1 in proliferate endometrium and endometrial carcinoma was significantly higher than that in normal endometrium ( P<0.05,P<0.01) .The overexpression of cyclin D 1 in the atypical hyperplasia group was obviously different from that in the simple and compound hyperplasia group ( P <0.01).In endometrial carcinoma,the expression of p16 was decreasing with the descending of cell differentiate degree, on the opposite, the expression of cyclin D 1 was increased and there existed a negative correlation between them.It was also observed that the overexpression of cyclin D 1 was significant different between G 1 and G 2,G 3(P<0.01).Conclusion p16 is a negative regulating factor of cell cycle in endometrial carcinoma, while cyclin D 1 is a positive one.Both of them are important in the genesis and development of endometrial carcinoma.The low expression of p16 and the overexpression of cyclin D 1 are related with the malicious biological behaviors of endometrial carcinoma and maybe play an important role in the judgement of prognosis.Overexpression of cyclin D 1 may be an earlier molecular event in the genesis of endometrial carcinoma.展开更多
Objective： To investigate the expressions of caveolin-1, E-cadherin and β-catenin in gastric carcinoma, precancerous gastric and chronic non-atrophic gastritis tissues, and evaluate the correlation of these expressi...Objective： To investigate the expressions of caveolin-1, E-cadherin and β-catenin in gastric carcinoma, precancerous gastric and chronic non-atrophic gastritis tissues, and evaluate the correlation of these expressions with the development of gastric cancer. Methods： The expressions of caveolin-1, E-cadherin and β-catenin were detected by biotin-streptavidinperoxidase （SP） immunohistochemistry on 58 gastric cancer tissues, 40 precancerous gastric tissues and 42 chronic non-atrophic gastritis tissues. The correlation between the expressions of caveolin-1, E-cadherin and β-catenin, and the clinicopathologic parameters of gastric cancer was analyzed retrospectively. Results： The positive rates of caveolin-1 and E-cadherin expressions in gastric carcinoma were significantly lower than precancerous gastric and chronic non-atrophic gastritis tissues （P〈0.01）. An abnormal rate of β-catenin expression in gastric carcinoma was higher than precancerous gastric and chronic non-atrophic gastritis tissues （P〈0.01）. Moreover, low expressions of caveolin-1, E-cadherin and β-catenin correlated with tumor size, depth of invasion, lymph node metastasis and TNM stage （P〈0.05）. The positive rates of caveolin-1 and E-cadherin expressions decreased （P〈0.01）, while an abnormal rate of β-catenin expression increased inversely, with the degree of atypical hyperplasia （P〈0.01）. Caveolin-1 expression correlated positively with E-cadherin （r=0.41, P〈0.05）. Caveolin-1 （r=-0.36, P〈0.05） and E-cadherin （r=-0.45, P〈0.05） expressions negatively correlated with abnormal β-catenin expression. Conclusion： These results suggested that dysregulated expressions of caveolin-1, E-cadherin and β-catenin correlated with the development of gastric cancer and its biological behavior.展开更多
文摘Objective: To investigate the expression of Smad4 and p21WAF1 in endometrial carcinoma and its clinical significance. Methods: Immunohistochemical method wasused to detect Smad4 and p21WAF1 expression in 56 cases of endometrial carcinoma. Results: The positive rate ofSmad4 was 80.36% in endometrial carcinoma. The Samd4 expression was significantly correlated with histologicalgrade (P<0.01) and clinical stage (P<0.05). The positive rate of p21WAF1 was 64.28% in endometrial carcinoma. Theexpression of p21WAF1 was correlated with depth ofmyometrial invasion (P<0.01). There was no correlation between Smad4 and p21WAF1 expression (P>0.05). Conclusion: Smad4 may play an important role in the tumorigenesis, differentiation and progression ofendometrial carcinoma. The expression of p21WAF1 wasassociated with the tumorigenesis of endometrial carcinoma, but the association between p21WAF1 and differentiationand progression of endometrial carcinomas needs to befurther investigated.
文摘Objective To study the role of p16 and cyclin D 1 in the genesis and development of endometrial carcinoma.Methods 12 cases of normal endometrium,22 cases of proliferative endometrium and 41 cases of endometrial carcinoma were detected for the expression of p16 and cyclin D 1 by means of immunohistochemical S P. Results In normal endometrium p16 was expressed while cyclin D 1 was almost negative in the proliferative phase,but both of them were negative in the secretory phase.Among the groups of the simple and compound hyperplasia, the atypical hyperplasia and the endometrial carcinoma,the expression of p16 showed a descending tendency, while the expression of cyclin D 1 showed an ascending tendency.In endometrial carcinomas the expression of p16 was significantly lower than that of normal endometrium and proliferative endometrium( P <0.01, P <0.05).However, the expression of cyclin D 1 in proliferate endometrium and endometrial carcinoma was significantly higher than that in normal endometrium ( P<0.05,P<0.01) .The overexpression of cyclin D 1 in the atypical hyperplasia group was obviously different from that in the simple and compound hyperplasia group ( P <0.01).In endometrial carcinoma,the expression of p16 was decreasing with the descending of cell differentiate degree, on the opposite, the expression of cyclin D 1 was increased and there existed a negative correlation between them.It was also observed that the overexpression of cyclin D 1 was significant different between G 1 and G 2,G 3(P<0.01).Conclusion p16 is a negative regulating factor of cell cycle in endometrial carcinoma, while cyclin D 1 is a positive one.Both of them are important in the genesis and development of endometrial carcinoma.The low expression of p16 and the overexpression of cyclin D 1 are related with the malicious biological behaviors of endometrial carcinoma and maybe play an important role in the judgement of prognosis.Overexpression of cyclin D 1 may be an earlier molecular event in the genesis of endometrial carcinoma.
基金supported by Zhejiang Provincial Natural Science Foundation (No. Y206750)Zhejiang Foundation for Development of Science and Technology, China (No. 2008C33066)Wenzhou Foundation for Development of Science and Technology,China (No. H20060026)
文摘Objective： To investigate the expressions of caveolin-1, E-cadherin and β-catenin in gastric carcinoma, precancerous gastric and chronic non-atrophic gastritis tissues, and evaluate the correlation of these expressions with the development of gastric cancer. Methods： The expressions of caveolin-1, E-cadherin and β-catenin were detected by biotin-streptavidinperoxidase （SP） immunohistochemistry on 58 gastric cancer tissues, 40 precancerous gastric tissues and 42 chronic non-atrophic gastritis tissues. The correlation between the expressions of caveolin-1, E-cadherin and β-catenin, and the clinicopathologic parameters of gastric cancer was analyzed retrospectively. Results： The positive rates of caveolin-1 and E-cadherin expressions in gastric carcinoma were significantly lower than precancerous gastric and chronic non-atrophic gastritis tissues （P〈0.01）. An abnormal rate of β-catenin expression in gastric carcinoma was higher than precancerous gastric and chronic non-atrophic gastritis tissues （P〈0.01）. Moreover, low expressions of caveolin-1, E-cadherin and β-catenin correlated with tumor size, depth of invasion, lymph node metastasis and TNM stage （P〈0.05）. The positive rates of caveolin-1 and E-cadherin expressions decreased （P〈0.01）, while an abnormal rate of β-catenin expression increased inversely, with the degree of atypical hyperplasia （P〈0.01）. Caveolin-1 expression correlated positively with E-cadherin （r=0.41, P〈0.05）. Caveolin-1 （r=-0.36, P〈0.05） and E-cadherin （r=-0.45, P〈0.05） expressions negatively correlated with abnormal β-catenin expression. Conclusion： These results suggested that dysregulated expressions of caveolin-1, E-cadherin and β-catenin correlated with the development of gastric cancer and its biological behavior.
