BACKGROUND Hepatitis B virus(HBV)infection is a major factor responsible for HBV+hepatocellular carcinoma(HCC).AIM An immunological classification of HBV+HCC may provide both biological insights and clinical implicati...BACKGROUND Hepatitis B virus(HBV)infection is a major factor responsible for HBV+hepatocellular carcinoma(HCC).AIM An immunological classification of HBV+HCC may provide both biological insights and clinical implications for this disease.METHODS Based on the enrichment of 23 immune signatures,we identified two immunespecific subtypes(Imm-H and Imm-L)of HBV+HCC by unsupervised clustering.We showed that this subtyping method was reproducible and predictable by analyzing three different datasets.RESULTS Compared to Imm-L,Imm-H displayed stronger immunity,more stromal components,lower tumor purity,lower stemness and intratumor heterogeneity,lower-level copy number alterations,higher global methylation level,and better overall and disease-free survival prognosis.Besides immune-related pathways,stromal pathways(ECM receptor interaction,focal adhesion,and regulation of actin cytoskeleton)and neuro-related pathways(neuroactive ligand-receptor interaction,and prion diseases)were more highly enriched in Imm-H than in Imm-L.We identified nine proteins differentially expressed between Imm-H and Imm-L,of which MYH11,PDCD4,Dvl3,and Syk were upregulated in Imm-H,while PCNA,Acetyl-a-Tubulin-Lys40,ER-α_pS118,Cyclin E2,andβ-Catenin were upregulated in Imm-L.CONCLUSION Our data suggest that“hot”tumors have a better prognosis than“cold”tumors in HBV+HCC and that“hot”tumors respond better to immunotherapy.展开更多
Analysis of 2547 cases of multiple myeloma (MM)reported in China in 1980s sbowed that the clinical manifestations are characterized by multipliey. High misdiagnosis rate (69%) and multiple complications.while the mono...Analysis of 2547 cases of multiple myeloma (MM)reported in China in 1980s sbowed that the clinical manifestations are characterized by multipliey. High misdiagnosis rate (69%) and multiple complications.while the monoclonal protein had more than 25immunological types, IgG myeloma was the commonest (43.1%). Light chain subgroup trended to have a higher intidence of renal demage (76.9%). Plasma cell leukemia evenetually developed in 30 cases. In order to improve diagnosis and avoid misdiagnosis, the key points are 1, to better the recognition of clinical features of MM. 2.Patient should reteive urine Bence-Jones Protein,immunoglobulins, immunoelectropbresis, bone X-ray and multiple site bone marrow puncture whenever one of such manifestations as unexplained anemia, skeletal pain,proteinuria, elevation of ESR, hyperviscosity syndrome,hypercalcemia, hyperuricemia, clevatiou of alkaline phosphatase, pathological fractures and diffuse osteoporosis, 3. Immuno-binding clectrophoresis and immunofluorence antibody detection should be done for suspected cases with normal immunoglobulin level.展开更多
文摘BACKGROUND Hepatitis B virus(HBV)infection is a major factor responsible for HBV+hepatocellular carcinoma(HCC).AIM An immunological classification of HBV+HCC may provide both biological insights and clinical implications for this disease.METHODS Based on the enrichment of 23 immune signatures,we identified two immunespecific subtypes(Imm-H and Imm-L)of HBV+HCC by unsupervised clustering.We showed that this subtyping method was reproducible and predictable by analyzing three different datasets.RESULTS Compared to Imm-L,Imm-H displayed stronger immunity,more stromal components,lower tumor purity,lower stemness and intratumor heterogeneity,lower-level copy number alterations,higher global methylation level,and better overall and disease-free survival prognosis.Besides immune-related pathways,stromal pathways(ECM receptor interaction,focal adhesion,and regulation of actin cytoskeleton)and neuro-related pathways(neuroactive ligand-receptor interaction,and prion diseases)were more highly enriched in Imm-H than in Imm-L.We identified nine proteins differentially expressed between Imm-H and Imm-L,of which MYH11,PDCD4,Dvl3,and Syk were upregulated in Imm-H,while PCNA,Acetyl-a-Tubulin-Lys40,ER-α_pS118,Cyclin E2,andβ-Catenin were upregulated in Imm-L.CONCLUSION Our data suggest that“hot”tumors have a better prognosis than“cold”tumors in HBV+HCC and that“hot”tumors respond better to immunotherapy.
文摘Analysis of 2547 cases of multiple myeloma (MM)reported in China in 1980s sbowed that the clinical manifestations are characterized by multipliey. High misdiagnosis rate (69%) and multiple complications.while the monoclonal protein had more than 25immunological types, IgG myeloma was the commonest (43.1%). Light chain subgroup trended to have a higher intidence of renal demage (76.9%). Plasma cell leukemia evenetually developed in 30 cases. In order to improve diagnosis and avoid misdiagnosis, the key points are 1, to better the recognition of clinical features of MM. 2.Patient should reteive urine Bence-Jones Protein,immunoglobulins, immunoelectropbresis, bone X-ray and multiple site bone marrow puncture whenever one of such manifestations as unexplained anemia, skeletal pain,proteinuria, elevation of ESR, hyperviscosity syndrome,hypercalcemia, hyperuricemia, clevatiou of alkaline phosphatase, pathological fractures and diffuse osteoporosis, 3. Immuno-binding clectrophoresis and immunofluorence antibody detection should be done for suspected cases with normal immunoglobulin level.
