目的探讨Survivin、胰岛素样生长因子ⅡmRNA结合蛋白3(insulin-like growth factorⅡmRNA-binding protein 3,IMP3)及Claudin1在浆膜腔积液细胞蜡块中的表达及临床意义。方法收集我院病理科2017年2月~2021年11月行免疫组化检查且临床资...目的探讨Survivin、胰岛素样生长因子ⅡmRNA结合蛋白3(insulin-like growth factorⅡmRNA-binding protein 3,IMP3)及Claudin1在浆膜腔积液细胞蜡块中的表达及临床意义。方法收集我院病理科2017年2月~2021年11月行免疫组化检查且临床资料齐全的浆膜腔积液细胞块95例,其中恶性84例,良性11例(对照组)。应用免疫组化方法检测浆膜腔积液细胞蜡块中Survivin、IMP3及Claudin1蛋白的表达情况,并对比分析其在良恶性浆膜腔积液以及不同恶性肿瘤中的差异。结果恶性胸膜腔积液病例中胸腔积液53例,腹腔积液31例。肺腺癌41例,消化系统腺癌25例,卵巢恶性肿瘤9例,其他恶性肿瘤9例。与对照组相比,肺腺癌组及消化系统腺癌组IMP3的表达差异具有统计学意义(P<0.05),消化系统腺癌组Claudin1的表达差异具有统计学意义(P<0.05),其他组别Survivin、IMP3及Claudin1的表达与对照组相比差异均无统计学意义(P>0.05)。结论IMP3及Claudin1在恶性浆膜腔积液判断是否为消化系统腺癌来源时具有鉴别意义,IMP3在肺腺癌来源具有鉴别意义。展开更多
Objective: Class B carbapenemases are bacterial enzymes that catalyze the hydrolysis of β-lactam core antibiotics, except for monobactams. The objective of this study was to identify the carbapenemase gene bla<sub...Objective: Class B carbapenemases are bacterial enzymes that catalyze the hydrolysis of β-lactam core antibiotics, except for monobactams. The objective of this study was to identify the carbapenemase gene bla<sub>IMP</sub> in the genus Klebsiella at the Charles De Gaulle Pediatric University Hospital (CHUP-CDG) of Ouagadougou, Burkina Faso. Methods: The study involved 17 bacterial strains responsible for human infection and isolated from various biological samples during the period from 2009 to 2013. The strains were tested for antimicrobial susceptibility to cefotaxime, ceftazidime and imipenem using the Mueller-Hinton agar diffusion method. The carbapenemases resistance genes were detected by conventional PCR using specific primers at the molecular biology laboratory of CERBA/LABIOGENE, Ouagadougou, Burkina Faso. Results: The antibiotic susceptibility test showed high resistance of the 17 Klebsiella isolates tested to cephalosporins. A high cefotaxime-resistance rate (82.35%) and ceftazidime-resistance rate (88.23%) was found among the strains tested against 11.76% resistance rate for imipenem. Analysis of PCR products by gel electrophoresis revealed 4 strains (23.53%) with bla<sub>IMP</sub>-type gene. Conclusion: Klebsiella is a well-known bacterium in clinical practice. The present study demonstrated the bla<sub>IMP</sub>-type gene in cephalosporin-resistant strains of Klebsiella at CHUP-CDG. More effective monitoring and treatment solutions are needed to prevent the spread of these resistant strains.展开更多
目的:探讨胰岛素样生长因子Ⅱm RNA结合蛋白3(IMP3)及胰岛素样生长因子-2(IGF2)在不同类型胶质瘤中的表达及意义。方法:采用免疫组化S-P法观察98例胶质瘤标本及20例非瘤脑组织中IMP3和IGF2蛋白的表达情况,分析二者的表达与不同临床病理...目的:探讨胰岛素样生长因子Ⅱm RNA结合蛋白3(IMP3)及胰岛素样生长因子-2(IGF2)在不同类型胶质瘤中的表达及意义。方法:采用免疫组化S-P法观察98例胶质瘤标本及20例非瘤脑组织中IMP3和IGF2蛋白的表达情况,分析二者的表达与不同临床病理特征的关系;并随机选取IMP3及IGF2不同程度阳性表达病例行IMP3和IGF2 m RNA原位杂交检测。结果:在不同类型胶质瘤组织中IMP3蛋白、IGF2蛋白及IMP3 m RNA均呈高表达,除少突胶质细胞瘤外,二者在Ⅲ级肿瘤组织中的表达水平均明显高于Ⅱ级(P<0.05);在胶质母细胞瘤中二者的表达水平最高。IGF2 m RNA在不同类胶质瘤中均有表达,但在Ⅱ级、Ⅲ级肿瘤组织及胶质母细胞瘤之间表达差异无统计学意义(P>0.05)。IMP3、IGF2蛋白的阳性表达水平与患者年龄、肿瘤病理级别及术后生存时间关系密切(P<0.05)。结论:IMP3和IGF2对胶质瘤的恶性进展、患者预后存在一定影响,两者可能成为脑胶质瘤诊断和治疗的特异靶点。展开更多
目的探讨胰岛素样生长因子IIm RNA结合蛋白3(insulin-like growth factor-II m RNA-binding protein 3,IMP3)及胰岛素样生长因子-2(Insulin-like growth factor-2,IGF2)在胶质瘤中的表达及对临床预后的影响。方法采用免疫组化S-P法检测...目的探讨胰岛素样生长因子IIm RNA结合蛋白3(insulin-like growth factor-II m RNA-binding protein 3,IMP3)及胰岛素样生长因子-2(Insulin-like growth factor-2,IGF2)在胶质瘤中的表达及对临床预后的影响。方法采用免疫组化S-P法检测171例脑胶质瘤组织及20例非瘤脑组织中IMP3、IGF2及Ki-67的表达情况;分析IMP3、IGF2及Ki-67表达强度间的相关性;Kaplan-Meier生存分析及多变量Cox比例风险回归模型分析胶质瘤患者生存状况的影响因素。结果非瘤脑组织、胶质瘤组织中IMP3、IGF2和Ki-67的表达水平差异均有统计学意义(P均<0.05)。且胶质瘤组织中IMP3、IGF2蛋白及Ki-67表达均随肿瘤病理级别升高而增高(P均<0.05)。Spearman相关分析显示,IMP3、IGF2与Ki-67表达水平皆呈正相关,且IMP3蛋白的表达与IGF2蛋白的表达水平呈正相关(P均<0.05)。Kaplan-Meier分析结果显示IMP3、IGF2的表达与临床预后密切相关(P<0.05)。Cox比例风险回归模型分析示,肿瘤病理分级、IMP3、IGF2及Ki-67均为胶质瘤患者预后的独立危险因素(P均<0.05)。结论 IMP3和IGF2蛋白的表达与胶质瘤的组织病理分级、肿瘤细胞增殖及患者临床预后密切相关,针对二者的靶点治疗有望成为胶质瘤的有效治疗方案。展开更多
文摘目的探讨Survivin、胰岛素样生长因子ⅡmRNA结合蛋白3(insulin-like growth factorⅡmRNA-binding protein 3,IMP3)及Claudin1在浆膜腔积液细胞蜡块中的表达及临床意义。方法收集我院病理科2017年2月~2021年11月行免疫组化检查且临床资料齐全的浆膜腔积液细胞块95例,其中恶性84例,良性11例(对照组)。应用免疫组化方法检测浆膜腔积液细胞蜡块中Survivin、IMP3及Claudin1蛋白的表达情况,并对比分析其在良恶性浆膜腔积液以及不同恶性肿瘤中的差异。结果恶性胸膜腔积液病例中胸腔积液53例,腹腔积液31例。肺腺癌41例,消化系统腺癌25例,卵巢恶性肿瘤9例,其他恶性肿瘤9例。与对照组相比,肺腺癌组及消化系统腺癌组IMP3的表达差异具有统计学意义(P<0.05),消化系统腺癌组Claudin1的表达差异具有统计学意义(P<0.05),其他组别Survivin、IMP3及Claudin1的表达与对照组相比差异均无统计学意义(P>0.05)。结论IMP3及Claudin1在恶性浆膜腔积液判断是否为消化系统腺癌来源时具有鉴别意义,IMP3在肺腺癌来源具有鉴别意义。
文摘Objective: Class B carbapenemases are bacterial enzymes that catalyze the hydrolysis of β-lactam core antibiotics, except for monobactams. The objective of this study was to identify the carbapenemase gene bla<sub>IMP</sub> in the genus Klebsiella at the Charles De Gaulle Pediatric University Hospital (CHUP-CDG) of Ouagadougou, Burkina Faso. Methods: The study involved 17 bacterial strains responsible for human infection and isolated from various biological samples during the period from 2009 to 2013. The strains were tested for antimicrobial susceptibility to cefotaxime, ceftazidime and imipenem using the Mueller-Hinton agar diffusion method. The carbapenemases resistance genes were detected by conventional PCR using specific primers at the molecular biology laboratory of CERBA/LABIOGENE, Ouagadougou, Burkina Faso. Results: The antibiotic susceptibility test showed high resistance of the 17 Klebsiella isolates tested to cephalosporins. A high cefotaxime-resistance rate (82.35%) and ceftazidime-resistance rate (88.23%) was found among the strains tested against 11.76% resistance rate for imipenem. Analysis of PCR products by gel electrophoresis revealed 4 strains (23.53%) with bla<sub>IMP</sub>-type gene. Conclusion: Klebsiella is a well-known bacterium in clinical practice. The present study demonstrated the bla<sub>IMP</sub>-type gene in cephalosporin-resistant strains of Klebsiella at CHUP-CDG. More effective monitoring and treatment solutions are needed to prevent the spread of these resistant strains.
文摘目的:探讨胰岛素样生长因子Ⅱm RNA结合蛋白3(IMP3)及胰岛素样生长因子-2(IGF2)在不同类型胶质瘤中的表达及意义。方法:采用免疫组化S-P法观察98例胶质瘤标本及20例非瘤脑组织中IMP3和IGF2蛋白的表达情况,分析二者的表达与不同临床病理特征的关系;并随机选取IMP3及IGF2不同程度阳性表达病例行IMP3和IGF2 m RNA原位杂交检测。结果:在不同类型胶质瘤组织中IMP3蛋白、IGF2蛋白及IMP3 m RNA均呈高表达,除少突胶质细胞瘤外,二者在Ⅲ级肿瘤组织中的表达水平均明显高于Ⅱ级(P<0.05);在胶质母细胞瘤中二者的表达水平最高。IGF2 m RNA在不同类胶质瘤中均有表达,但在Ⅱ级、Ⅲ级肿瘤组织及胶质母细胞瘤之间表达差异无统计学意义(P>0.05)。IMP3、IGF2蛋白的阳性表达水平与患者年龄、肿瘤病理级别及术后生存时间关系密切(P<0.05)。结论:IMP3和IGF2对胶质瘤的恶性进展、患者预后存在一定影响,两者可能成为脑胶质瘤诊断和治疗的特异靶点。
