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Market Disease Pathogens Detection of Imported Fruits in Shanghai 被引量:5
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作者 MA Teng-fei YANG Bo +9 位作者 YU Yue WANG Yi-wen LIU Yi XU Zhen LIU Yan ZHU Pin-kuan ZHANG Wei ZHANG Zai-bao Toyoda Hideyoshi XU Ling 《Agricultural Sciences in China》 CAS CSCD 2009年第9期1087-1096,共10页
A tremendous amount of imported fresh fruits has been delivered to Shanghai markets, increasing the risk of invasion by harmful plant pathogens. Therefore, it is important to establish an effective detection and super... A tremendous amount of imported fresh fruits has been delivered to Shanghai markets, increasing the risk of invasion by harmful plant pathogens. Therefore, it is important to establish an effective detection and supervision system to survey the outbreak of the market diseases of the imported fruits during marketing. The samples were regularly surveyed in different markets to examine varieties, prices, localities, selling conditions, and diseases of the imported fruits from 2004 to 2008. The survey showed that 58 species of 30 different fruits were imported to Shanghai from 16 countries with more expensive price. The larger products were bananas, grapes, apples, and oranges. During the investigation, we found that the imported fruits frequently brought about the relatively serious market diseases. On the basis of morphology and the nuclear internal transcribed spacer (ITS) of ribosomal DNA (rDNA) analysis, 151 isolates of 15 fungi genera, which shown to be pathogenic after the inoculation assay, were finally identified. Among the identified fungi, Alternaria was the most frequent one with the highest detection rate (47.68%), followed by Penicillium (14.57%) and Fusarium (11.92%), respectively. Additionally, Pestalotiopsis microspora (detected in grapes Red-Globe coming from the USA) and Botrytis sp. (detected in black-plums coming from the USA) were first reported in China market. The present study summarized the selling situation of the imported fruits in Shanghai markets and constructed a library of the pathogens detected in the imported fruits during the selling period. The results obtained are useful to offer technical parameters for Chinese quarantine in order to prevent an invasion of the foreign harmful micro-organisms. 展开更多
关键词 imported fruits market research market diseases pathogens
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上海市进口火龙果软腐病病害分析 被引量:20
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作者 崔志婧 王奕文 +1 位作者 于岳 许玲 《微生物学通报》 CAS CSCD 北大核心 2011年第10期1499-1506,共8页
应用病原形态学和真菌rDNA-ITS分子标记及Fusarium oxysporum种特异性序列分析,对引起上海市水果市场销售的进口火龙果软腐的市场病害进行了分离与鉴定,明确了两种主要的致病真菌为尖孢镰刀菌(Fusarium oxysporum)和单隔镰刀菌(Fusarium... 应用病原形态学和真菌rDNA-ITS分子标记及Fusarium oxysporum种特异性序列分析,对引起上海市水果市场销售的进口火龙果软腐的市场病害进行了分离与鉴定,明确了两种主要的致病真菌为尖孢镰刀菌(Fusarium oxysporum)和单隔镰刀菌(Fusarium dimerum)。其中,F.oxysporum为引起进口火龙果采后软腐的最主要病原真菌,该菌最适生长温度和致病温度均为25°C,光照条件下的致病性强,在15°C和35°C条件下致病性明显降低,5°C和45°C条件下该菌无法正常生长和致病,并且该菌还能够引起香蕉、番茄、葡萄等多种水果腐烂。系统研究引起进口火龙果采后软腐病病原真菌的生物学特性,为进口火龙果采后病原真菌有效控制方法的制定提供了有益的参考。 展开更多
关键词 进口水果 市场病害 镰刀菌 分子标记 生物学特性
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