The activation and development of primordial follicles is the key to the maturation of female gametes.Premature ovarian insufficiency(POI)patients are unable to complete the primordial follicle activation and developm...The activation and development of primordial follicles is the key to the maturation of female gametes.Premature ovarian insufficiency(POI)patients are unable to complete the primordial follicle activation and development due to follicular dormancy and unbalanced developmental regulation in the body,leading to female infertility.Ovarian tissue in vitro activation(IVA)technology has become a new way to solve the problem of patients who cannot auto-activate primordial follicles to obtain their own mature oocytes.In IVA research,signaling pathways such as PI3K/PTEN/Akt and Hippo have become the focus of current research.This review will describe the relevant research progress and clinical application of the IVA mechanism,and provide a reference for clinical research on ovarian tissue culture and activation in vitro.展开更多
Young female cancer survival rates significantly increased due to the great progress of cancer therapy.In fact,cryostorage and transplantation of ovarian tissue have already resulted in the birth of healthy babies.Fol...Young female cancer survival rates significantly increased due to the great progress of cancer therapy.In fact,cryostorage and transplantation of ovarian tissue have already resulted in the birth of healthy babies.Follicle in vitro growth(IVG)has the great potential of restoring fertility by achieving functional oocytes from the most immature stages to maturation.This is suitable for a wide range of patients,from pubertal to perimenopause women.Notable achievements have been achieved in human follicle IVG in the past decade.Mature oocytes have been successfully collected from long-term sequential follicle IVG.However,it is still a major challenge to establish a stable and efficient follicle IVG system able to generate mature and competent oocytes.Hereby,we review the approaches being taken so far using ovarian tissue to support follicle growth at different stages in vitro.展开更多
Premature ovarian failure(POF) is a refractory disease for clinical treatment with the goal of restoring fertility. In this study,umbilical cord mesenchymal stem cells on a collagen scaffold(collagen/UC-MSCs) can acti...Premature ovarian failure(POF) is a refractory disease for clinical treatment with the goal of restoring fertility. In this study,umbilical cord mesenchymal stem cells on a collagen scaffold(collagen/UC-MSCs) can activate primordial follicles in vitro via phosphorylation of FOXO3 a and FOXO1. Transplantation of collagen/UC-MSCs to the ovaries of POF patients rescued overall ovarian function, evidenced by elevated estradiol concentrations, improved follicular development, and increased number of antral follicles. Successful clinical pregnancy was achieved in women with POF after transplantation of collagen/UC-MSCs or UC-MSCs. In summary, collagen/UC-MSC transplantation may provide an effective treatment for POF.展开更多
基金Major Science and Technology Projects in Hainan Province(ZDKJ2017007)Hainan Natural Science Foundation(2019CXTD408)。
文摘The activation and development of primordial follicles is the key to the maturation of female gametes.Premature ovarian insufficiency(POI)patients are unable to complete the primordial follicle activation and development due to follicular dormancy and unbalanced developmental regulation in the body,leading to female infertility.Ovarian tissue in vitro activation(IVA)technology has become a new way to solve the problem of patients who cannot auto-activate primordial follicles to obtain their own mature oocytes.In IVA research,signaling pathways such as PI3K/PTEN/Akt and Hippo have become the focus of current research.This review will describe the relevant research progress and clinical application of the IVA mechanism,and provide a reference for clinical research on ovarian tissue culture and activation in vitro.
文摘Young female cancer survival rates significantly increased due to the great progress of cancer therapy.In fact,cryostorage and transplantation of ovarian tissue have already resulted in the birth of healthy babies.Follicle in vitro growth(IVG)has the great potential of restoring fertility by achieving functional oocytes from the most immature stages to maturation.This is suitable for a wide range of patients,from pubertal to perimenopause women.Notable achievements have been achieved in human follicle IVG in the past decade.Mature oocytes have been successfully collected from long-term sequential follicle IVG.However,it is still a major challenge to establish a stable and efficient follicle IVG system able to generate mature and competent oocytes.Hereby,we review the approaches being taken so far using ovarian tissue to support follicle growth at different stages in vitro.
基金supported by Strategic Priority Research Program of the Chinese Academy of Sciences (XDA01030501 to Haixiang Sun)National Natural Science Foundation of China (31571189, 81571391, 30900847 to Lijun Ding)+1 种基金Jiangsu Provincial Medical Youth Talent (QNRC2016006)Nanjing Medical Science Development Project (JQX14004, ZKX16042)
文摘Premature ovarian failure(POF) is a refractory disease for clinical treatment with the goal of restoring fertility. In this study,umbilical cord mesenchymal stem cells on a collagen scaffold(collagen/UC-MSCs) can activate primordial follicles in vitro via phosphorylation of FOXO3 a and FOXO1. Transplantation of collagen/UC-MSCs to the ovaries of POF patients rescued overall ovarian function, evidenced by elevated estradiol concentrations, improved follicular development, and increased number of antral follicles. Successful clinical pregnancy was achieved in women with POF after transplantation of collagen/UC-MSCs or UC-MSCs. In summary, collagen/UC-MSC transplantation may provide an effective treatment for POF.
