In vitro androgenesis is an important component of plant biotechnology when the pollen grains are forced to switch from their normal pollen developmental pathway towards an embryogenic route. Haploid and doubled haplo...In vitro androgenesis is an important component of plant biotechnology when the pollen grains are forced to switch from their normal pollen developmental pathway towards an embryogenic route. Haploid and doubled haploid produced through androgenesis have long been recognized as a valuable tool in plant breeding as it can shorten the breeding cycle, fix agronomic characters in homozygous state and enhance the selection efficiency of useful recessive agronomic traits. Recently, doubled haploids have been largely recognized as an important component of crop improvement through genome mapping, quantitative trait locus analysis, and genetic mutation, and as targets for genetic transformation programs. Thus, this review is focused mainly on various facets of doubled haploid in the chief staple food crop rice and sights its recent applications in plant breeding, genetics and genomics.展开更多
Sorghum[Sorghum bicolor(L.)Moench]can benefit from accelerated breeding and release of improved varieties through doubled haploid technology.The technology has been used in speeding up the breeding of other major cere...Sorghum[Sorghum bicolor(L.)Moench]can benefit from accelerated breeding and release of improved varieties through doubled haploid technology.The technology has been used in speeding up the breeding of other major cereals such as wheat,maize and rice,for which generally widely applied optimised protocols exist.A reproducible protocol for the crop,that can overcome genotype dependency and other species-specific challenges such as phenolic exudation is however lacking.This study aimed at sorghum doubled haploids production thereby contributing to the development of an improved protocol.From the 28 hybrid genotypes,both F1 registered-and experimental hybrids involved,this study successfully produced haploids from five genotypes and subsequently,four confirmed doubled-haploid lines on W14mf medium or its modification with 1.0 gl^(-1)L-proline,1.0 gl^(-1)L-asparagine and 1.0 gl^(-1)KH_(2)PO_(4).Medium 190-2Cu was used for regeneration and rooting,which occurred successfully,if the calli were transferred on to it less than 7 days after induction,and temperature was maintained at 25°C under light condition.Genotype dependency was not wholly overcome;however,sorghum’s high tillering ability and abiotic stress tolerance were observed to contribute to attainment of haploid plantlets.Spontaneous diploids producing seeds at rates of upto 80.5%were obtained,therefore eliminating the need for colchicine duplication.展开更多
文摘In vitro androgenesis is an important component of plant biotechnology when the pollen grains are forced to switch from their normal pollen developmental pathway towards an embryogenic route. Haploid and doubled haploid produced through androgenesis have long been recognized as a valuable tool in plant breeding as it can shorten the breeding cycle, fix agronomic characters in homozygous state and enhance the selection efficiency of useful recessive agronomic traits. Recently, doubled haploids have been largely recognized as an important component of crop improvement through genome mapping, quantitative trait locus analysis, and genetic mutation, and as targets for genetic transformation programs. Thus, this review is focused mainly on various facets of doubled haploid in the chief staple food crop rice and sights its recent applications in plant breeding, genetics and genomics.
基金This work was supported through the Stipendium Hungaricum Scholarship to the first author,János Bolyai Research Scholarship of the Hungarian Academy of Sciences,as well as research grants(TUDFO/51757/2019-ITM and K_16-K119835).
文摘Sorghum[Sorghum bicolor(L.)Moench]can benefit from accelerated breeding and release of improved varieties through doubled haploid technology.The technology has been used in speeding up the breeding of other major cereals such as wheat,maize and rice,for which generally widely applied optimised protocols exist.A reproducible protocol for the crop,that can overcome genotype dependency and other species-specific challenges such as phenolic exudation is however lacking.This study aimed at sorghum doubled haploids production thereby contributing to the development of an improved protocol.From the 28 hybrid genotypes,both F1 registered-and experimental hybrids involved,this study successfully produced haploids from five genotypes and subsequently,four confirmed doubled-haploid lines on W14mf medium or its modification with 1.0 gl^(-1)L-proline,1.0 gl^(-1)L-asparagine and 1.0 gl^(-1)KH_(2)PO_(4).Medium 190-2Cu was used for regeneration and rooting,which occurred successfully,if the calli were transferred on to it less than 7 days after induction,and temperature was maintained at 25°C under light condition.Genotype dependency was not wholly overcome;however,sorghum’s high tillering ability and abiotic stress tolerance were observed to contribute to attainment of haploid plantlets.Spontaneous diploids producing seeds at rates of upto 80.5%were obtained,therefore eliminating the need for colchicine duplication.
