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Observation and Analysis of in vitro Expression of Mouse Heart Nuclear DNA Fragments by AFM
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作者 袁明秀 Hou +6 位作者 Zhonggang Wang Feng Zhang Zhihong Hong Wei 《High Technology Letters》 EI CAS 2001年第2期6-7,共4页
Using AFM,we observed linear chain-like complexes formed by some specific proteins and the multi-mRNAs during the in vitro expression of some active genes on the DNA fragments. The LDH mRNA in the multi-mRNA complex c... Using AFM,we observed linear chain-like complexes formed by some specific proteins and the multi-mRNAs during the in vitro expression of some active genes on the DNA fragments. The LDH mRNA in the multi-mRNA complex can in vitro translate LDH. Via AFM, we also discovered that nmRNA prepared from heart muscles, along with some specific proteins can form linear chain-like nmRNA complexes in which LDH mRNA can also translate LDH in vitro. Our work shows the prospective application of AFM in the research of the biological reaction of the active genes on the DNA fragments. 展开更多
关键词 in vitro expression nmrna linear chain-like complex group effect afm
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