Objective To investigate clinical outcomes in patients who were at more precise criteria risks for fertilization failure and were treated with selective, short-term fertilization(oocytes and sperm co-incubated for 4 h...Objective To investigate clinical outcomes in patients who were at more precise criteria risks for fertilization failure and were treated with selective, short-term fertilization(oocytes and sperm co-incubated for 4 h) and early rescue intracytoplasmic sperm injection(ICSI).Methods A retrospective analysis was performed on 2 023 women undergoing assisted reproductive technology(ART). They were assigned to 4 groups: short-term in vitro fertilization(short-term IVF, group A, n=217), regular IVF(oocytes and sperm coincubated overnight, group B, n=1 475), short-term IVF and early rescue ICSI(shortterm ICSI, group C, n=94), and regular ICSI(group D, n=237).Results In group A, 69.8%(217/311) achieved normal fertilization rates, and the complete fertilization failure rate(fertilization rate was 0%) was 12.9%(40/311). But all of the fertilization failure oocytes got rescue ICSI. In group B, the complete fertilization failure rate was 1.1%(19/1 692). The fertilization rate, 2 PN(pronucleus)rate, and 1 PN rate were significantly lower in group A than those in group B(70.9%vs 80.8%, 57.8% vs 66.3%, and 3.5% vs 6.2%, respectively). No significant differences were observed in clinical pregnancy rates and birth defect rates between groups A and B. The fertilization rates in groups C and D did not significantly differ(77.9% vs76.2%), which was also true for birth defect rates. The clinical pregnancy rate of group C was higher than that of group D(51.2% vs 42.3%), but this difference was not significant(P>0.05).Conclusion These results suggested that selective, short-term fertilization can result in effective outcomes for patients who were at high risk for fertilization failure.展开更多
目的:探讨200 IU hCG在控制性卵巢刺激(COS)过程的晚卵泡期替代hMG对COS的疗效。方法:回顾性分析行体外受精/单精子卵胞质内注射-冻融胚胎移植(IVF/ICSI-FET)患者资料共154例,进行154个COS周期,根据晚卵泡期是否应用200 IU hCG分为:A组,...目的:探讨200 IU hCG在控制性卵巢刺激(COS)过程的晚卵泡期替代hMG对COS的疗效。方法:回顾性分析行体外受精/单精子卵胞质内注射-冻融胚胎移植(IVF/ICSI-FET)患者资料共154例,进行154个COS周期,根据晚卵泡期是否应用200 IU hCG分为:A组,COS完全应用hMG(65个周期);B组,COS的早卵泡期应用hMG,晚卵泡期则应用hCG(200 IU/d)替代hMG(89个周期)。后续166个周期进行FET,其中,A组70个周期,B组96个周期。统计分析COS周期的用药情况、IVF/ICSI-FET结局。结果:B组的hMG用药剂量和用药时间分别显著少于A组(1 361.0±494.6 IU vs 1 782.7±475.2 IU,P<0.05;7.3±2.3 d vs 9.5±2.0 d,P<0.05);B组的成熟卵母细胞数显著多于A组(15.2±6.6 vs 11.6±5.7,P<0.05);冻融胚胎移植中A、B组的临床妊娠率(64.29%vs 64.58%,P>0.05)及活产率(80.00%vs 79.03%,P>0.05)比较无统计学差异。结论:200 IU hCG能够在COS的晚卵泡期替代hMG,能安全、有效地维持卵泡生长发育,并且减少Gn的用量,避免卵巢过度刺激综合征(OHSS)发生。展开更多
基金supported by Science and Information Technology of Guangzhou(2012Y2-00022)
文摘Objective To investigate clinical outcomes in patients who were at more precise criteria risks for fertilization failure and were treated with selective, short-term fertilization(oocytes and sperm co-incubated for 4 h) and early rescue intracytoplasmic sperm injection(ICSI).Methods A retrospective analysis was performed on 2 023 women undergoing assisted reproductive technology(ART). They were assigned to 4 groups: short-term in vitro fertilization(short-term IVF, group A, n=217), regular IVF(oocytes and sperm coincubated overnight, group B, n=1 475), short-term IVF and early rescue ICSI(shortterm ICSI, group C, n=94), and regular ICSI(group D, n=237).Results In group A, 69.8%(217/311) achieved normal fertilization rates, and the complete fertilization failure rate(fertilization rate was 0%) was 12.9%(40/311). But all of the fertilization failure oocytes got rescue ICSI. In group B, the complete fertilization failure rate was 1.1%(19/1 692). The fertilization rate, 2 PN(pronucleus)rate, and 1 PN rate were significantly lower in group A than those in group B(70.9%vs 80.8%, 57.8% vs 66.3%, and 3.5% vs 6.2%, respectively). No significant differences were observed in clinical pregnancy rates and birth defect rates between groups A and B. The fertilization rates in groups C and D did not significantly differ(77.9% vs76.2%), which was also true for birth defect rates. The clinical pregnancy rate of group C was higher than that of group D(51.2% vs 42.3%), but this difference was not significant(P>0.05).Conclusion These results suggested that selective, short-term fertilization can result in effective outcomes for patients who were at high risk for fertilization failure.
文摘目的:探讨200 IU hCG在控制性卵巢刺激(COS)过程的晚卵泡期替代hMG对COS的疗效。方法:回顾性分析行体外受精/单精子卵胞质内注射-冻融胚胎移植(IVF/ICSI-FET)患者资料共154例,进行154个COS周期,根据晚卵泡期是否应用200 IU hCG分为:A组,COS完全应用hMG(65个周期);B组,COS的早卵泡期应用hMG,晚卵泡期则应用hCG(200 IU/d)替代hMG(89个周期)。后续166个周期进行FET,其中,A组70个周期,B组96个周期。统计分析COS周期的用药情况、IVF/ICSI-FET结局。结果:B组的hMG用药剂量和用药时间分别显著少于A组(1 361.0±494.6 IU vs 1 782.7±475.2 IU,P<0.05;7.3±2.3 d vs 9.5±2.0 d,P<0.05);B组的成熟卵母细胞数显著多于A组(15.2±6.6 vs 11.6±5.7,P<0.05);冻融胚胎移植中A、B组的临床妊娠率(64.29%vs 64.58%,P>0.05)及活产率(80.00%vs 79.03%,P>0.05)比较无统计学差异。结论:200 IU hCG能够在COS的晚卵泡期替代hMG,能安全、有效地维持卵泡生长发育,并且减少Gn的用量,避免卵巢过度刺激综合征(OHSS)发生。
